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Citations for Millipore Sigma :
9851 - 9900 of 10000+ citations for Recombinant Human SERPINF1 Protein since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2023Quote: ... each sample with equal amounts of protein was subjected to SDS–PAGE and transferred to a PVDF membrane (Millipore, Burlington, MA). The membranes were incubated with the primary antibody at 4 ℃ overnight ...
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bioRxiv - Molecular Biology 2023Quote: ... The purified protein was then polished by a size exclusion chromatography step using a Superdex ® 200 Increase 10/300 GL (Sigma) in storage buffer (10 mM Tris-HCl pH 7.4 ...
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bioRxiv - Physiology 2023Quote: ... For protein extraction the tissue was homogenised in RIPA buffer with a cocktail of phosphatase inhibitors (Sigma-Aldrich, Merck, Gillingham, Dorset), using a sonicator three times for 30s each time ...
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bioRxiv - Plant Biology 2023Quote: ... Forty µg of total protein from each sample was resolved in 12% SDS gel and blotted onto a PVDF membrane (Millipore, USA). The blot was blocked with 5% non-fat milk in TBST buffer (50 mM Tris–HCl ...
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bioRxiv - Molecular Biology 2023Quote: LX-2 cells were lysed and proteins were extracted using radioimmunoprecipitation assay buffer supplemented with complete EDTA-free protease inhibitor cocktail (Sigma-Aldrich). After centrifugation of cell lysates at 12,000 x g for 10 min ...
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bioRxiv - Cell Biology 2023Quote: ... Protein content was quantified by Bradford assay54 and 20 μg of protein of each cell line were separated by SDS-PAGE and electrotransferred to PVDF membranes (Millipore Sigma). The primary antibodies anti-ZIP11 and anti-GAPDH as a loading control ...
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bioRxiv - Neuroscience 2023Quote: ... HPC and PFC samples were homogenized in tissue protein extraction reagent (TPER) [10 mL/g] and protease inhibitor cocktail (Millipore Sigma) [10 µL/mL] ...
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bioRxiv - Immunology 2023Quote: ... were formed by dissociating and maintaining PSCs in ESC media supplemented with 10 μM Rho-associated protein kinase (ROCK) inhibitor (Sigma, Y0503) while being kept on an orbital shaker at 100 rpm for 6 days to allow for spontaneous formation of EBs with hematopoietic potential ...
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bioRxiv - Biochemistry 2023Quote: ... and the bound proteins were eluted with buffer H containing 0.1 M NaCl and 100 μg/mL of FLAG peptide (Sigma Aldrich, #F3290). Peak fractions were pooled and loaded onto a MonoQ 5/50GL column (Cytiva ...
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bioRxiv - Biophysics 2023Quote: ... purified in the form of fusion proteins by Ni-NTA affinity purification and further digested by using the SUMO Protease (Sigma-Aldrich) according to the protocols provided by the manufacturer ...
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bioRxiv - Cell Biology 2023Quote: ... Equivalent amounts of denatured protein homogenate were resolved by SDS-PAGE on 10% polyacrylamide gels and transferred to a polyvinylidene difluoride membrane (Millipore, USA). After blocking at room temperature for 2 h ...
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bioRxiv - Immunology 2023Quote: ... Protein was further purified by size exclusion chromatography using gel-filtration column (Superose 6 increase 10/300 GL, Cytiva, Sigma-Aldrich) in a buffer containing 50 mM Tris-HCl pH 8.0 ...
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bioRxiv - Microbiology 2023Quote: ... The upper part was used for protein detection using a specific monoclonal antibody against Cas9 (1:1000 dilution, #MAC133, Sigma-Aldrich) and Alexa555 secondary antibody for fluorescence detection (iBright ...
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bioRxiv - Biochemistry 2023Quote: ... the supernatant proteins were denatured at 95℃ for 10 min and then alkylated with 40 mM IAA (final concentration, Sigma-Alrich) in dark at room temperature for 30 min ...
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bioRxiv - Neuroscience 2023Quote: ... Pull down of Ago proteins (Ago 1-4) was performed by incubating 500 μg of Flag-GST-T6B peptide 111 (Sigma #M8823) with 80 μl of anti-Flag M2 Magnetic beads (Sigma #M8823 ...
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bioRxiv - Biophysics 2023Quote: ... Aggregation of mutant was carried out with a protein concentration of 100 µM in 10 mM phosphate buffer (Sigma-Aldrich, USA), pH 7.4 at 37ºC without agitation.
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bioRxiv - Cell Biology 2022Quote: ... Selected fraction with pure fusion protein was concentrated and buffer exchanged using an Amicon® Ultra Centrifugal Filter Unit 50MWCO (Millipore) into Extract Compatible Buffer (10 mM HEPES pH7.8 ...
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bioRxiv - Cell Biology 2022Quote: ... Hxk2-3V5 and associated proteins were immunoprecipitated from glass bead extracts (250 μg total protein) using 20 μL of agarose conjugated anti-V5 antibody (Sigma; A7345). The extract and beads were incubated at 4°C overnight ...
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bioRxiv - Cell Biology 2022Quote: ... The proteins were eluted from the beads by 25 µL 1x NuPAGE LDS sample buffer supplemented to 100 mM DTT (Sigma-Aldrich) and heated for 10 min at 70 °C ...
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bioRxiv - Cancer Biology 2023Quote: ... An equal amount of protein (10μg) per sample was separated on 10% SDS polyacrylamide gels and then transferred onto PVDF membranes (Millipore, MA, USA). The membrane was blocked with 4% non-fat milk in TBST buffer at room temperature for 2 hours ...
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bioRxiv - Cancer Biology 2023Quote: ... Protein extracts were separated using a 10% sodium dodecyl sulfate polyacrylamide gel and transferred to immobilon polyvinylidene difluoride membranes (EMD Millipore). After blocking for one hour at room temperature in odyssey blocking buffer (LI-COR Biosciences) ...
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bioRxiv - Cancer Biology 2023Quote: ... Thirty μg of protein lysates were separated using SDS–polyacrylamide gel electrophoresis and transferred to a PVDF membrane (Immobilion-P, Millipore, IPVH00010). After blocking the membranes in 5% BSA in TBST (1× TBS (Cell Signalling) ...
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bioRxiv - Cancer Biology 2023Quote: ... Approximately 1.5 mg of proteins from each sample was reduced with TCEP (5 mM, room temperature for 10 min, Cat# 580561, Millipore Sigma) then alkylated with chloroacetamide (20 mM ...
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bioRxiv - Cell Biology 2023Quote: ... elegans ChIP reaction or from 50% of a control extract (1 mg protein) were de-crosslinked at 65 °C for at least 4 h with 150 μg/ml Proteinase K (Sigma, #3115887001). DNA from each ChIP reaction or from control extract was isolated using the Qiagen PCR purification kit and diluted to a final volume of 200 μl with (10 mM Tris-HCl ...
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bioRxiv - Cell Biology 2023Quote: ... The samples for mass spectrometry (MS) analysis were incubated with Dynabeads protein G that were precoupled with the M2-Flag-antibody (Sigma, F1804) at 4°C overnight ...
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bioRxiv - Cell Biology 2023Quote: ... MBP- and GST-tagged proteins were purified by gel filtration on a Superdex 200 column (Sigma-Aldrich Cat# GE17-5175-01) and concentrated using a 30K Amicon filter.
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bioRxiv - Genomics 2023Quote: ... total cellular ERAP2 protein was enriched by immunoprecipitation and incubated with L-Arginine-7-amido-4-methylcoumarin hydrochloride (R-AMC, A2027, Sigma-Aldrich). Briefly ...
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bioRxiv - Cell Biology 2023Quote: ... the column was washed with 20 column volumes of the lysis buffer and the protein was eluted with 3 mM desthiobiotin (Sigma, D1411).
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bioRxiv - Biochemistry 2023Quote: ... One half was used for protein extraction and the other half used for UA quantification using a UA Assay Kit (MAK077, Sigma-Aldrich). Cells were disrupted mechanically ...
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bioRxiv - Physiology 2023Quote: Proteins of interest were labeled with well-validated custom and commercial antibodies: Connexin43 (Cx43; mouse monoclonal antibody, Cat. #: MAB3067, EMD Millipore Corp. ...
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bioRxiv - Cancer Biology 2022Quote: Polyacrylamide gel slices containing bEV and sEV protein lysates were resolved in tris (2-carboxyethyl) phosphine (10 × 10-1 M; Sigma- Aldrich) with ammonium bicarbonate (50 × 10-1 M ...
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bioRxiv - Microbiology 2023Quote: ... and the amount of total protein in each sample was calculated using a 1 mg/ml standard sample of bovine serum albumin (Sigma-Aldrich). To separate the soluble and aggregated proteins ...
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bioRxiv - Neuroscience 2023Quote: ... Protein was filtered with a 0.22 µm filter and concentrated using Amicon Ultra-15 centrifugal filter units (Millipore Sigma Cat#UFC901008). Protein was then loaded onto a Superdex 200 column and 5 mL fractions were collected ...
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bioRxiv - Cancer Biology 2023Quote: ... 80 µg of equivalent proteins from sample were treated with 5mM tris (2-carboxyethyl) phosphine hydrochloride (Sigma-Aldrich Cat No: C4706) for 15 minutes at 37°C to reduce the disulfide bonds ...
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bioRxiv - Immunology 2023Quote: ... The capture matrix was washed three times with PBS and incubated over night with 1:5 diluted plasma samples in a low protein binding MultiScreen® filter plate (Millipore). Depleted plasma was recovered by centrifugation of the filter plate ...
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bioRxiv - Cell Biology 2023Quote: ... Equal amounts of protein were separated by SDS-PAGE and transferred to polyvinylidene difluoride (PVDF) membrane (Immobilion-P, Millipore, Schwalbach, Germany) by semi-dry blotting ...
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bioRxiv - Molecular Biology 2023Quote: ... The FLAG-tagged protein was detected by incubating the milk powder-blocked membrane with mouse monoclonal anti-FLAG primary antibody (Sigma-Aldrich) diluted at 1:1000 ...
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bioRxiv - Cell Biology 2023Quote: ... and SKR-2-containing protein complexes were eluted overnight in 100 µl of the lysis buffer containing 2 mg/mL of 3xFlag peptide (Sigma F4799). Eluted protein samples were digested with trypsin and analyzed by MudPIT in the Mass Spectrometry and Proteomics Facility at Johns Hopkins School of Medicine.
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bioRxiv - Plant Biology 2023Quote: ... protein thiol groups were blocked by a final concentration of freshly balanced 20 mM N-ethylmaleimide (NEM, Sigma 128-53-0) directly added to the lysis buffer ...
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bioRxiv - Biophysics 2023Quote: ... Proteins were concentrated by centrifugation in 4 mL of Amicon filter concentrators with a 10 kDa cutoff (Millipore Sigma; Burlington, MA). TCEP (1 mM ...
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bioRxiv - Neuroscience 2023Quote: ... Sections were then blocked and permeabilized in protein-blocking buffer (DPBS with 2% BSA, 10% fish gelatin (cat#: G7041, Sigma-Aldrich), 0.2% Triton-X (cat# T9284 ...
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bioRxiv - Neuroscience 2023Quote: ... Then 10 mg of protein per sample was separated on 12% SDS-PAGE gels (Sangon Biotech) and subsequently transferred to PVDF membrane (Millipore, Temecula) using sodium dodecyl sulfate-polyacrylamide gel electrophoresis and Western blotting techniques ...
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bioRxiv - Molecular Biology 2023Quote: ... was diluted to 0.01 mg/mL concentration in 100 µL of 25 mM Tris/HCl buffer containing various concentrations of SAHS proteins or BSA (Sigma-Aldrich). 50 µL of each sample was stored at 4°C while the other 50 µL was dried using a Savant SPD131DDA SpeedVac (Thermo Scientific ...
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bioRxiv - Genomics 2023Quote: ... the remaining chromatin was divided amongst separate tubes containing protein A dynabeads pre-incubated with either 2μl anti-H3K4me3 (Millipore 07-473), 10μl anti-H3K27me3 (CST 9733 ...
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bioRxiv - Microbiology 2023Quote: ... and the flow-through containing cleaved protein was collected and concentrated to 2 mL by ultrafiltration (Amicon Ultra-15, EMD Millipore), then passed over a size exclusion column (HiLoad Superdex 200 PG ...
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bioRxiv - Neuroscience 2023Quote: ... solubilized GluA2 or GluA2-γ2 (1 nmol) and membrane scaffold proteins (50 μL, final concentration 1 mg/mL) (MSP1E3D1, Sigma‒ Aldrich) were added ...
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bioRxiv - Biochemistry 2023Quote: ... The protein was incubated for 1h at 4 °C in the presence or absence of 20 or 100 µM FTY720 (Sigma-Aldrich) or fumonisin B1 (Sigma-Aldrich) ...
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bioRxiv - Biochemistry 2023Quote: Studies of SAMHD1-controled dynamic equilibria of dNTP concentrations (Fig. 5) were performed using a 10 mL stirred-cell pressurized protein concentrator (Amicon; EMD Millipore) assembled with a 10 kDa MWCO nitrocellulose membrane (Millipore) ...
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bioRxiv - Biochemistry 2023Quote: Clicked and dissolved protein samples were diluted to 4 M urea with 50 mM ammonium bicarbonate (pH 8, AmBic, Sigma-Aldrich). The proteins were reduced with 4 mM DTT (Sigma-Aldrich ...
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bioRxiv - Bioengineering 2023Quote: To generate emulsions, 1 mL of fluorinated oil (Novec 7500, ACOTA) and 2 mL of protein solution (β-lactoglobulin; >90%, Sigma, from bovine milk ...