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Citations for Millipore Sigma :
9751 - 9800 of 10000+ citations for Recombinant Human SERPINF1 Protein since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2020Quote: ... Equivalent amounts of total protein were loaded into a 12% SDS-PAGE for electrophoresis and then transferred into a PVDF membrane (Millipore, USA). After blocking nonspecific binding with 5% skimmed milk in TBST ...
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bioRxiv - Cell Biology 2021Quote: ... the TEV cleavage step was done overnight at 4°C and the cleaved proteins were concentrated using 100K MWCO concentrator (EMD Millipore). Cleaved proteins were filtered by centrifuging with Ultrafree-MC VV filter (EMD Millipore ...
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bioRxiv - Microbiology 2019Quote: Interactions between the SRB1 and NS1 proteins from DENV and ZIKV in Huh-7 and C6/36 cells were detected using the commercial kit Duolink (Sigma-Aldrich) based on in situ proximity ligation assay (PLA ...
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bioRxiv - Immunology 2021Quote: ... an amount of 15 μg of proteins was loaded on to a 96-well plate with a polyvinylidene fluoride (PVDF) membrane at the bottom (Millipore-Sigma), which had been previously activated and primed ...
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bioRxiv - Immunology 2021Quote: ... RNA was isolated from cells using Trizol® and protein was isolated from cell lysates with RIPA buffer (Sigma-Aldrich®) containing Triton X100 1% and SDS 0.1% ...
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bioRxiv - Molecular Biology 2021Quote: ... The purity of the protein after each step was assessed by 12% SDS-PAGE stained with Coomassie Brilliant Blue R 250 (Sigma-Aldrich). First ...
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bioRxiv - Molecular Biology 2020Quote: ... 10 μg of protein was reduced with 10 mM dithiothreitol (DTT; FUJIFILM Wako) and alkylated with 40 mM 2-chloroacetamide (CAA; Sigma-Aldrich). Digestion was performed according to the PTS protocol (Chang et al. ...
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bioRxiv - Microbiology 2021Quote: ... The FLAG-tagged protein-DNA complex was purified via affinity chromatography using anti-FLAG M2 affinity gel (Sigma-Aldrich, Taufkirchen, Germany). The purified protein-DNA complexes and the control were incubated at 95 °C for 40 min for reversal of the crosslink ...
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bioRxiv - Bioengineering 2021Quote: ... Equal amounts of protein were subject to SDS-PAGE and blotted with anti-Rho antibody (3 μg/mL, #05-778, EMD Millipore) or anti-GAPDH antibody (1:1000 dilution ...
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bioRxiv - Cell Biology 2021Quote: ... The covalently fluorescently labelled nucleosome arrays used for microinjection were labeled at an 8% fluorophore density (8 in 100 histone2B proteins labeled with a fluorophore) and dialyzed against TE (10 mM Tris-HCl (Sigma-Aldrich), pH 7.4 (Sigma-Aldrich) ...
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bioRxiv - Genetics 2020Quote: ... proteins were eluted by boiling in 1XSDS-PAGE loading buffer followed by immunoblotting analysis using anti-FLAG antiboy (Sigma; Cat# A8592).
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bioRxiv - Microbiology 2020Quote: ... The temperature was subsequently reduced to 18°C and protein production was induced with 0.5 mM Isopropyl-β-D-thiogalactoside (IPTG, Sigma Aldrich) overnight ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... Samples were centrifuged and protein concentration measured using a bovine serum albumin standard curve and a bicinchoninic acid (BCA) assay (Sigma-Aldrich). The WES capillary electrophoresis system (ProteinSimple ...
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bioRxiv - Biochemistry 2020Quote: The effect of the mutations on translational control in response to stress was assessed by measuring the incorporation of puromycin into newly synthesized proteins by immunoblotting lysates of untreated and thapsigargin (Sigma, T9033) (200 nM ...
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bioRxiv - Cell Biology 2021Quote: ... 500 µg of lysate proteins were incubated with 2 µl of anti-Flag monoclonal antibody pre-adsorbed on protein A-Sepharose beads (cat. no. A2220, Sigma-Aldrich) overnight at 4°C.
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bioRxiv - Neuroscience 2022Quote: Hippocampal tissues from P40 WT mice were collected and lysed with IP lysis buffer in EZ-Magna RIP™ RNA-Binding Protein Immunoprecipitation Kit (Merck-Millipore). YTHDF2 (Proteintech ...
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bioRxiv - Developmental Biology 2022Quote: ... FLAG-tagged constructs were then immunoprecipitated with Dynabeads Protein G beads coupled to mouse anti-FLAG M2 mAb (Sigma #F3165, RRID:AB_259529) for 3 hours at 4°C with rotation ...
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bioRxiv - Cell Biology 2022Quote: ... Conditional degradation of mAID tagged EWSR1 protein in the AUX treated OsTIR7-19 cells was confirmed by Immunocytochemistry and Western blot analysis using anti-FLAG (Sigma, #F7425) and anti-EWSR1 (Santa Cruz ...
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bioRxiv - Cancer Biology 2022Quote: ... purified the protein using Ni- resin and exchanged the bound nucleotide for non-hydrolysable GTP analogue GMPPCP using alkaline phosphatase beads (Sigma-Aldrich) and following the protocol of John et.al ...
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bioRxiv - Cancer Biology 2022Quote: ... Proteins were fractionated by 10-15% SDS gels and electro-transferred to polyvinylidene difluoride transfer membranes (#IPVH00010, Millipore, Billerica, MA, USA). The membrane was incubated overnight with primary antibodies and then with secondary antibodies conjugated with horseradish peroxidase (#7074S and #7076S ...
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bioRxiv - Biochemistry 2022Quote: ... and the major fractions containing the target protein were pooled and then concentrated with an Amicon® Ultra-15 Centrifugal Filter Unit (10 kDa cutoff, Millipore) to ~500 mL ...
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bioRxiv - Bioengineering 2022Quote: ... hexane-defatting was performed before extraction by adding 10 g of protein meal to 100 mL of hexane (Sigma #270504-1L) and incubating the mixtures at room temperature on a stir-plate for one hour ...
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bioRxiv - Cancer Biology 2022Quote: Thrombin (human plasma, high activity) and trypsin (porcine pancreas, Type IX-S, 13000-20000 BAEE units/mg protein) was purchased from Millipore Sigma. PARs activating peptides TFLLR-NH2 (PAR1) ...
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bioRxiv - Cell Biology 2022Quote: ... and GST proteins were eluted in GST elution buffer (50 mM Tris-HCl pH 8, 10 mM glutathione (Sigma-Aldrich; G4251). The supernatant was separated from the resin to a fresh tube ...
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bioRxiv - Cell Biology 2022Quote: The assay was performed using the Click-iT™ HPG Alexa Fluor™ 488 Protein Synthesis Assay Kit (# C10428, Sigma Aldrich) according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2022Quote: ... the beads were removed and protein of interest was concentrated using a 100 kDa molecular weight cut-off (MWCO) concentrator (Millipore, #Z648043) to 1 mL and flash-frozen in liquid nitrogen.
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bioRxiv - Developmental Biology 2022Quote: ... Activity of the TCF3-VP16GR chimeric protein was induced by incubating the embryos for 24 hours in 4 μg/ml dexamethasone (DEX, Sigma-Aldrich) from stage 37/38 to stage 41.
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bioRxiv - Immunology 2022Quote: Reduced cell extracts (20 µg of protein) or immunoprecipitates were resolved by SDS-PAGE and transferred to PVDF membranes (Merck-Millipore, #10344661) using a Trans-Blot transfer cell (BioRad) ...
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bioRxiv - Genetics 2022Quote: ... TAP-tagged Rrp45 protein was detected with peroxidase anti-peroxidase (PAP) soluble complex antibody produced in rabbit (1:5000, Sigma-Aldrich). The 3-phosphoglycerate kinase (Pgk1 ...
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bioRxiv - Plant Biology 2022Quote: ... The membranes were washed with Tris buffered saline containing 0.1% Tween-20 and probed again for protein loading control using a 1:10000 dilution of mouse monoclonal anti-actin (plant) primary antibody (Sigma, A0480) and a 1:3000 dilution of goat anti-mouse IgG HRP conjugate (Biorad).
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TOR acts as metabolic gatekeeper for auxin-dependent lateral root initiation in Arabidopsis thalianabioRxiv - Plant Biology 2022Quote: ... 20 µl protein extract were separated on a 12% SDS gel and transferred to Nitrocellulose membrane (Sigma-Aldrich, St. Louis, MO). Anti-S6K1 (phospho T449 ...
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bioRxiv - Microbiology 2022Quote: ... diluted 1:5000 to detect RVFV and anti-Gc protein antibody (4C12A1) diluted 1:5000 to detect LACV with FFA staining buffer (1X PBS, 1mg/ml saponin (Sigma: 47036)) as a primary detection antibody overnight at 4°C ...
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bioRxiv - Microbiology 2022Quote: ... ∼2×107 parasites were collected by centrifugation (1,200 x g for 10 min) and pull-downs were performed using the Magna RIP RNA Binding Protein Immunoprecipitation Kit (Sigma-Aldrich). Unenriched ‘input’ samples were generated by reserving back 10% of the starting lysate ...
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bioRxiv - Molecular Biology 2022Quote: ... cells were washed twice with cold phosphate-buffer saline (PBS) and total proteins were precipitated in 5% trichloroacetic acid (TCA) with 1 mM Methionine (Met) (Sigma-Aldrich) for 15 minutes on ice ...
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bioRxiv - Neuroscience 2022Quote: ... The concentration of each GalNAcT-FLAG enzyme was measured by performing immunoblot analysis together with standard FLAG-BAP fusion protein (Sigma-Aldrich) using the M2 antibody ...
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bioRxiv - Immunology 2022Quote: ... The sections were then incubated overnight with primary antibodies directed against SARS Nucleocapsid protein (1/500; mouse monoclonal; clone 1C7C7; Sigma-Aldrich), ionised calcium-binding adapter molecule 1 (Iba1 ...
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bioRxiv - Microbiology 2022Quote: ... Chromatin extracts containing DNA fragments with an average size of 500 bp were immunoprecipitated with the indicated antibodies or rabbit preimmune sera complexed with Protein A agarose beads (Sigma-Aldrich). Immunoprecipitated complexes were sequentially washed with Wash Buffer A ...
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bioRxiv - Microbiology 2022Quote: ... Debris were later removed by centrifugation and 20 μl of the cleared protein sample were spiked-in with 10 μl of UPS2 spike-in (Sigma-Aldrich) diluted in 250 μl of 1.25x protein loading buffer ...
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bioRxiv - Microbiology 2022Quote: ... Bound rVSVs were detected by incubation with a streptavidin-HRP conjugate (PeirceTM, and sEC1-4 protein was detected by incubation with an anti-Flag m2 mAb-HRP conjugate (Sigma-Aldrich) (1 h at 37°C) ...
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bioRxiv - Systems Biology 2022Quote: Caco-2 cell lysates expressing FLAG-KRAS proteins were immunoprecipitated from 800 μg of cell lysate using anti-FLAG-M2 magnetic beads (Sigma, M8823) by using the KingFisher DuoPrime purification system (ThermoFisher) ...
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bioRxiv - Molecular Biology 2023Quote: ... Reagents for protein chemistry (e.g., triethylammonium bicarbonate buffer, iodoacetamide, dithiothreitol, and formic acid) were purchased from Sigma Aldrich (St. Louis, MO). Sequencing grade trypsin was from Promega (Madison WI) ...
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bioRxiv - Molecular Biology 2023Quote: ... V5-tagged protein was precipitated with 20 μl anti-V5 beads (Anti-V5 Agarose Affinity Gel, Cat.- No. A7345, Sigma-Aldrich) rotating on a wheel for 2 h at 4 °C ...
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bioRxiv - Microbiology 2023Quote: ... The interactions between the two labeled proteins were detected using the Duolink in Situ Red Starter Kit Mouse/Rabbit (#DUO92101-1kit, Sigma-Aldrich) according to the manufacturer’s instructions.
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bioRxiv - Physiology 2023Quote: ... 200μg protein was incubated with anti–c-Myc affinity gel (EZview™ Red Anti-c-Myc Affinity Gel, #E6654, Sigma-Aldrich) overnight ...
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bioRxiv - Neuroscience 2022Quote: ... 600 μg protein equivalents of exosomes pelleted by ultracentrifugation were resuspended in 500 μl Diluent-C for membrane labeling (Sigma CGLDIL) and then mixed 1:1 with 500 μl Diluent-C containing 2 μl CellVue Claret Far-Red Fluorescent Membrane Linker (Sigma MINCLARET) ...
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bioRxiv - Molecular Biology 2022Quote: Protein samples dissolved in Laemmli buffer were separated on SDS-PAGE gel and transferred to Immobilon-P PVDF membrane (Millipore, IPVH00010). Transferred protein samples were detected using following primary antibodies ...
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bioRxiv - Molecular Biology 2022Quote: ... Soluble proteins were hatched for 10 min at 55°C with tris (2-carboxyethyl) phosphine (Sigma-Aldrich, St. Louis, MO, USA), then alkylated for 15 min at RT in the dark with 10 mg/mL iodoacetamide (Sigma-Aldrich ...
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bioRxiv - Molecular Biology 2022Quote: ... the accumulated lysates were incubated with protein A/G magnetic beads (Thermo-Fisher) pre-coupled with anti-Flag antibody (M2 monoclonal antibody, Sigma-Aldrich) for 2 h at 4°C with rotation ...
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bioRxiv - Microbiology 2022Quote: ... One mg of total protein lysate was incubated with 40ul of Ezview Red ANTI-FLAG M2 Affinity Gel (Cat # F2426-1ML, Sigma-Aldrich) overnight at 4°C ...
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bioRxiv - Developmental Biology 2022Quote: ... Specific protein detection was performed using diluted primary antibodies as follows: anti-NDRG1 @ 1:5000 (Sigma-Aldrich, catalog# HPA006881, rabbit polyclonal); anti-GAPDH @1:5000 (GeneTex ...