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Citations for Millipore Sigma :
9451 - 9500 of 10000+ citations for Recombinant Human SERPINF1 Protein since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Physiology 2023Quote: ... The proteins were collected using 700 μl elution buffer (20 mM Na2PO4, 300 mM NaCl, 300 mM imidazole) (Sigma Aldrich) and dialyzed against MOPS buffer (30 mM MOPS ...
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bioRxiv - Immunology 2023Quote: ... Non-antigen specific IgG and RBD-specific IgG were isolated from serum and eluted RBD-specific antibodies respectively by protein G beads (Millipore). Purified IgG was denatured and treated with PNGase enzyme (New England Biolabs ...
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bioRxiv - Developmental Biology 2023Quote: ... 20-40 µg of total protein was separated on 7-10% SDS-PAGE polyacrylamide gels and transferred onto Immobilon PVDF membranes (Millipore). Membranes were blocked using 5% non-fat dry milk in TBS supplemented with 0.1% Tween-20 for 1 h at room temperature ...
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bioRxiv - Immunology 2023Quote: ... membrane at the bottom (Multiscreen HTS 0.45 μm Hydrophobic High Protein Binding Membrane 96-well Filtration plate (Product No. MSIPS4510; Millipore-Sigma) (65 ...
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bioRxiv - Immunology 2023Quote: ... Protein fractions containing pure Cbl-b protein as confirmed by SDS-PAGE were pooled and concentrated using 10 kDa cutoff spin columns (Millipore), and the final protein concentration determined using the NanoDrop UV-Vis spectrophotometer (Thermo Scientific) ...
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bioRxiv - Neuroscience 2023Quote: ... Protein quantification was normalized to the total protein for each sample determined after transfer to the membrane using Ponceau S solution (Sigma).
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bioRxiv - Neuroscience 2023Quote: ... Sections were processed with a free-floating section staining protocol for immunofluorescence using unconjugated primary antibodies (βIV-Tubulin, T7941, Sigma‒Aldrich, dilution 1:400; GFAP, glial fibrillary acidic protein, G-A-5, Sigma‒Aldrich ...
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bioRxiv - Cell Biology 2023Quote: ... anti-mNG (Vanderbilt Antibody and Protein Resource Core, VU527) (1:1000) or anti-ɑ-tubulin (Sigma-Aldrich Cat# T5168, RRID:AB_477579) (1:5000 ...
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bioRxiv - Biophysics 2023Quote: The LAF-1 protein coding sequence was ordered as a gBlock Gene Fragment (TM) and inserted into a pET28a(+) vector from Novagen. The LAF-1 mutants E398A (DAAD ...
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bioRxiv - Molecular Biology 2023Quote: Primary neurons were infected for 24 h then protein was extracted by sonication in RIPA buffer supplemented with Protease Inhibitor Cocktail (Sigma) and Phosphatase Inhibitor Cocktail 2 (Sigma) ...
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bioRxiv - Biophysics 2023Quote: ... The lysozyme protein solution was filtered by a 0.22 μm hydrophilic polyethersulfone (PES) membrane filter (Cat#SLGP033NS, Merck Millipore, USA). The filtered sample was stored in 1.0 mL aliquots at −45 °C until crystallization experiments were performed ...
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bioRxiv - Biochemistry 2023Quote: ... Proteins were eluted with 100 µL of elution buffer supplemented with 200 µg/mL 3× Flag-peptide (Sigma-Aldrich, #F4799) and stored at -80◦C until usage ...
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bioRxiv - Neuroscience 2023Quote: ... Normalized total proteins were analyzed by electrophoresis in 10% polyacrylamide gels and transferred to PVDF membranes (Millipore, Billerica, MA, USA). Membranes were blocked in 5% skim milk (BD Biosciences ...
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bioRxiv - Cancer Biology 2023Quote: RIP assay was performed following the manufacturer’s instructions (EZ-Magna RIP RNA-binding Protein Immunoprecipitation Kit, Sigma-Aldrich, #17-701). Antibody against eIF4G was used for the part of immunoprecipitation ...
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bioRxiv - Biochemistry 2023Quote: ... The beads were washed again with 800 μl binding buffer three times and the bound proteins were eluted with the binding buffer supplemented with 10 mM D-desthiobiotin (Sigma) and analyzed with SDS PAGE.
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bioRxiv - Developmental Biology 2023Quote: ... A small blood draw was carried out 4 weeks later to check progress and a booster injection of 100μg of Tin protein in 200 μl of Freund’s Incomplete Adjuvant (Sigma-Aldrich 344291) was carried out at four sites ...
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bioRxiv - Bioengineering 2023Quote: ... The fibrinogen stock solution is made by first preparing a 50 mg/mL stock from lyophilized bovine blood plasma protein (Millipore). It is reconstituted in a controlled manner by adding sterile PBS without Ca2+ and Mg2+ and keeping it at 37°C without agitation for 2–3h ...
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ANGPTL8 R59W variant influences inflammation through modulating NF-κB pathway under TNFα stimulationbioRxiv - Biochemistry 2023Quote: ... GAPDH was used as internal control for protein loading and detected using anti-GAPDH antibody (ab2302; Millipore, Temecula, CA, USA).
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bioRxiv - Biochemistry 2023Quote: ... FLAG-tagged L protein mutants were detected using horseradish peroxidase-conjugated anti-FLAG M2 antibody (1:9,000) (A8592; Sigma-Aldrich) and bands were visualised by chemiluminescence using Clarity Max Western ECL Substrate (Bio-Rad ...
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Arabidopsis actin-binding protein WLIM2A links PAMP-triggered immunity and cytoskeletal organizationbioRxiv - Plant Biology 2023Quote: ... Proteins were reduced at 37 °C for 1 hour with 10 mm Tris(2-carboxyethyl)phosphine (TCEP, C-4706 Sigma) in 100 mm NH4HCO3 ...
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bioRxiv - Molecular Biology 2023Quote: ... Cell lines stably expressing the WT/mutant proteins were selected using media supplemented with 5 μg/ml puromycin (Sigma-Aldrich). The reconstitution of mutant GSECs was assessed by SDS-PAGE/western blotting ...
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bioRxiv - Cell Biology 2023Quote: ... The protein extracts were cleared by 10-min centrifugation and then were incubated with anti-FLAG agarose beads (Sigma-Aldrich) for 16h at 4°C ...
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bioRxiv - Immunology 2023Quote: ... Protein was harvested from cell culture supernatants and purified by binding to a nickel-nitrilotriacetic acid (Ni-NTA) column (Novagen) followed by elution with 1M imidazole containing 0.2% octyl β-D-glucopyranoside ...
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Critical contribution of mitochondria in the development of cardiomyopathy linked to desmin mutationbioRxiv - Pathology 2023Quote: ... The quantity of extracellular vesicles was evaluated by measuring the protein concentrations that were measured using Bicinchoninic Acid Kit (Sigma). Cardiomyocytes or spheroids were treated with pelleted vesicles corresponding to 0.1 mg of proteins per 25 000 cells every two days from day 10 until day 24 after the initiation of differentiation.
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bioRxiv - Molecular Biology 2023Quote: ... the virus-containing supernatant was harvested by gentle pipetting and filtered with a low protein binding syringe filter (0.22 μM, EDM Millipore # SLGV033RB). The virus was frozen at -80 °C for long-term storage or transduced directly into the desired cells.
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bioRxiv - Neuroscience 2023Quote: ... All samples were heat-denatured for 5 min at 100°C and run on a 4-20% Criterion Tris-HCl Protein gel and transferred to PVDF membrane (Millipore). Membranes with muscle tissue homogenates were blocked in 5% non-fat dry milk in TBS/0.2% Triton X-100 and incubated overnight in Tau12 (Millipore Sigma ...
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bioRxiv - Developmental Biology 2023Quote: ... The resultant samples were load on SDS-PAGE and subjected to pIMAGO phospho-protein detection kit with fluor-680 (Sigma) detection kit and LI-COR Odyssey platform for western blot analyses of phosphorylated proteins (Millipore Sigma) ...
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bioRxiv - Microbiology 2023Quote: ... the eluted fractions were analysed using SDS-PAGE and concentrated using 10-kDa (for CslA or GlxA monomers) or 100-kDa (for protein complexes) cut-off centrifugal filters (Millipore).
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bioRxiv - Developmental Biology 2023Quote: ... HSF5-N2 and control IgG antibodies were crosslinked to 50 µl of protein A-Dynabeads (Thermo-Fisher) by DMP (Sigma). The antibody-crosslinked beads were added to the testis extracts prepared above ...
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bioRxiv - Microbiology 2023Quote: ... Protein samples were resolved by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) followed by transfer to PVDF membrane (Millipore). The membrane was blocked with 5 % skim milk in tris-buffered saline with 0.1% Tween20 (TBS-T ...
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bioRxiv - Immunology 2023Quote: ... and 24 hours later treated with SARS-CoV 2 Spike glycoprotein receptor binding domain protein (ScoV2-RBD, 2.8 μg/mL; Baltymas) with and without a specific mitochondrial superoxide scavenger MitoTempo (10 μM; Sigma-Aldrich) for 24 hours.
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bioRxiv - Microbiology 2023Quote: ... Protein extracts were separated by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) and electrophoretically transferred to nitrocellulose membranes (Millipore), and blocked in 5% nonfat dry milk ...
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bioRxiv - Microbiology 2023Quote: ... The insoluble matter was removed by centrifugation 10000×g for 5 min at 4 °C and protein was estimated using BCA kit (Sigma) according to manufacturer’s instructions.
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bioRxiv - Cell Biology 2023Quote: ... 10-30μg total protein was loaded and separated by 10% SDS-PAGE gel and transferred to PVDF membranes (Millipore; GVHP29325). Then ...
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bioRxiv - Cancer Biology 2023Quote: ... TMT-labeled samples were incubated in IP buffer consisting of 1% Nonidet P-40 with protein G agarose beads conjugated to 24 μg of 4G10 V312 IgG and 6 μg of PT-66 (P3300, Sigma) overnight at 4℃ ...
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bioRxiv - Cell Biology 2023Quote: Rho family GTPase proteins were expressed using a modified pGEX vector transformed into Rosetta-Gami B BL21(DE3) pLysS strains (Novagen). Protein was purified from these cells by freeze-thaw lysis in Hepes buffer (20mM Hepes ...
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bioRxiv - Cell Biology 2023Quote: Proteins were separated using sodium dodecyl sulfate-polyacrylamide gel electrophoresis and transferred to a polyvinylidene difluoride (PVDF) membrane (Immobilon, MILLIPORE). Membranes were blocked with 5% nonfat dry milk in PBS with 0.05% Tween20 (PBS-T ...
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bioRxiv - Biochemistry 2023Quote: ... The eluted holo-McyA2-(C-A-PCP) protein was pooled and concentrated to 15 mg/ml by ultrafiltration (Amicon Ultracel-10K, Millipore), then ...
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bioRxiv - Biochemistry 2023Quote: ... Proteins were separated on SDS-PAGE gels and analyzed by western blotting (anti-FLAG M2, 1:1,000 dilution; Sigma, F1804)
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Treg cells drive MYCN-mediated immunosuppression and tumor aggressiveness in high-risk neuroblastomabioRxiv - Cancer Biology 2023Quote: ... CAC-NIH-NA-02)42–44, or anti-zebrafish Ncam1 antibody (1:100, Institute for Protein Innovation) in PBS containing 10 U/mL Heparin (Sigma), 10% FBS ...
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bioRxiv - Biochemistry 2023Quote: ... Protein was eluted from the resin by applying 6 mL of wash buffer supplemented with 20 mM reduced glutathione (Sigma).
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bioRxiv - Molecular Biology 2023Quote: ... About 30 µg of total cell protein was separated on 8% SDS-PAGE and blotted onto Immobilon-P PVDF membrane (Millipore). The membrane was blocked in 0.2% I-Block (Tropix) ...
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bioRxiv - Systems Biology 2023Quote: ... was applied to the membrane for 30 min to the protein of interest and with Anti-GFP (Millipore-Sigma, 11814460001) for kinase expression ...
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bioRxiv - Systems Biology 2023Quote: ... was applied to the membrane for 30 min to the protein of interest and with Anti-GFP (Millipore-Sigma, 11814460001) for kinase expression ...
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bioRxiv - Neuroscience 2023Quote: ... A260/A280 = 0.6) capsid protein was concentrated using a 100kDa MWCO Amicon® Ultra-15 Centrifugal Filter Unit (EMD Millipore) to a final concentration of 1.4 mg/ml ...
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bioRxiv - Cell Biology 2024Quote: ... Proteins were fractionated by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) and transferred to PVDF membranes (EMD Millipore, IPVH00010), then blocked in Tween 0.1% Tris-buffered saline containing 5% bovine serum albumin (Tocris 5217 ...
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bioRxiv - Cell Biology 2024Quote: ... Overexpression of the fusion protein was induced by adding 1 mM isopropyl-D-thiogalactopyranoside (IPTG; Sigma Aldrich; St. Louis, MO) to the transformed cell culture ...
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bioRxiv - Microbiology 2024Quote: ... mice were intranasally administered with 3 µg of the RSP protein and 5 μg of cholera toxin (CT; Sigma-Aldrich) as immunological adjuvant ...
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bioRxiv - Bioengineering 2024Quote: ... Fractions that were supposed to contain the protein of the expected size were concentrated by ultrafiltration (10 kDa, UFC9010, Millipore), and aliquots were frozen at -20 °C until use.
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bioRxiv - Biochemistry 2024Quote: ... and the fractions with the highest concentrations of mutant PGA proteins were concentrated into PBS using Amicon Ultra-4 Centrifugal Filters with a 10 kDa molecular weight cutoff (Merck Millipore). This process resulted in a protein with a purity exceeding 70 % and a concentration of approximately 1 mg/mL ...