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Citations for Millipore Sigma :
9601 - 9650 of 10000+ citations for Recombinant Human SERPINF1 Protein since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Bioengineering 2021Quote: ... the BiTE proteins were buffered exchanged to PBS and concentrated using Amicon Ultra-15 ultracentrifugation 10kDa cutoff tubes (Millipore Sigma, #UFC901096D).
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bioRxiv - Bioengineering 2020Quote: ... Bound proteins were eluted by competition with four 2 x CV of DPBS containing 100 µg/mL FLAG peptide (Sigma Aldrich). Purified proteins were formulated in DPBS by buffer exchange through desalting columns (GE Healthcare) ...
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bioRxiv - Biochemistry 2020Quote: ... the lyophilized pellets of all samples were re-suspended in 12 ml MilliQ water and the proteins were concentrated on 3 kDa cutoff Amicon® Ultra 15 centrifugal filter units (Merck-Millipore) 5,000 x g at 4°C to reach a final volume of 0.5 ml ...
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bioRxiv - Biochemistry 2020Quote: ... on genomic DNA isolated from 3-week-old tissue-cultured plants as a template and then selected by northern blot analysis with a HT specific cDNA fragment as probe and subsequently by western blot analysis with antibodies specific to rat HT protein (Sigma-Aldrich).
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The E3 ubiquitin-protein ligase MDM2 is a novel interactor of the von Hippel-Lindau tumor suppressorbioRxiv - Biochemistry 2020Quote: ... 0.2mg of PNS were incubated for 4 hours at 4°C with 5 μl of protein A magnetic beads (Pierce Thermoscientific) pre-incubated (1 hour at RT) with 2 μg of anti-FLAG antibody (Sigma, F7425). Immune complexes were finally washed 3 times with lysis buffer and eluted by incubating the beads (5’ at 70°C ...
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bioRxiv - Neuroscience 2021Quote: ... and incubated for 1 h at RT with anti-microtubule associated protein 2 (MAP2) mouse monoclonal antibody (diluted 1:200 in PBS/gelatin; Sigma-Aldrich) and with anti-Tau rabbit polyclonal antibody (diluted 1:500 in PBS/gelatin ...
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bioRxiv - Neuroscience 2020Quote: ... Sections were then incubated for two nights at 4°C with primary antibodies against glial fibrillary acidic protein (GFAP; 1:5000; MAB360; Merck Millipore, USA), ionized calcium binding adaptor molecule 1 (Iba1 ...
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bioRxiv - Cancer Biology 2021Quote: ... Protein samples were resolved by electrophoresis on 12% SDS–polyacrylamide gels and electrophoretically transferred to polyvinylidene difluoride (PVDF) membranes (Millipore Corporation) for 20 min at 25 V with the Trans-Blot Turbo (Bio-Rad Laboratories) ...
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bioRxiv - Bioengineering 2020Quote: The stock fibrinogen solution was prepared by dissolving bovine fibrinogen protein crystals isolated from bovine plasma (cat. no. F8360, Sigma-Aldrich) in Dulbecco’s Phosphate-Buffered Saline solution (DPBS ...
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bioRxiv - Bioengineering 2020Quote: ... the stock thrombin solution was prepared by dissolving bovine thrombin protein powder isolated from bovine plasma (cat. no. T4648, Sigma-Aldrich) in DPBS ...
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bioRxiv - Cell Biology 2021Quote: ... The proteins were separated via sodium dodecyl sulfate–polyacrylamide gel electrophoresis and then transferred to a polyvinylidene difluoride membrane (Millipore, USA) followed by blocking with 5% nonfat milk ...
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bioRxiv - Biochemistry 2021Quote: ... protein amounts ranging from 50 and 100 μg of protein were diluted with H2O to perform 200 μL and an additional 200 μL of 8 M urea (Sigma-Aldrich), 75 mM Tris-HCl (Sigma-Aldrich) ...
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bioRxiv - Biochemistry 2020Quote: ... Twenty micrograms of each sample was run on NuPAGE 4–12% Bis-Tris and proteins were electroblotted onto PVDF membranes (Millipore, ISEQ00010), which were further processed as described in the immunoblotting section.
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bioRxiv - Biochemistry 2020Quote: ... according to the manufacturer’s instructions and as described previously.25 Labelled protein was separated from free dye by FPLC on a HiPrep 26/10 Desalting column (Sigma-Aldrich) with NS1-RBD Buffer ...
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bioRxiv - Cell Biology 2021Quote: ... The beta subunit of complex V and proteins bound to it were immunoprecipitated using an antibody to the beta subunit of ATP synthase (Millipore, MAB3494) in accordance with the Pierce™ Co-Immunoprecipitation Kit (Cat.No:26149) ...
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bioRxiv - Cell Biology 2021Quote: ... The GST-Cdc14 constructs were expressed at 16°C overnight and the protein purification followed the same procedure as for 6His-3Flag-Cdc14 using glutathione-Sepharose beads (Millipore-Sigma, 17075601) and the following buffers ...
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bioRxiv - Cell Biology 2021Quote: ... Lysates were adjusted to 1 mg/ml in lysis buffer and subject to overnight immunoprecipitation at 4°C with either USP7 or USP11 antibody and protein A agarose beads (Sigma-Aldrich), or with GFP-NanoTrap beads (prepared in house ...
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bioRxiv - Plant Biology 2020Quote: ... The resin was then washed three times with 100 μl of the same buffer to remove unbound proteins then subjected to SDS-PAGE and immunoblotting using anti-FLAG-HRP (Sigma Aldrich) or antibodies recognizing native NRPD1 ...
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bioRxiv - Plant Biology 2020Quote: ... the FLAG-tagged proteins were immunoprecipitated by incubation of the extracts with 5 μL of anti-FLAG antibody agarose beads (Sigma-Aldrich) for 1 hr at 4 °C with gentle shaking ...
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bioRxiv - Synthetic Biology 2021Quote: ... The eluted protein was concentrated and buffer exchanged into PBS (10% glycerol) using an Amicon Ultra-0.5 3K centrifugal filters (Millipore Sigma, #C82301) and then frozen at −80°C ...
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bioRxiv - Plant Biology 2020Quote: ... After washing 3 times with PBS protein was eluted from beads in Laemmli buffer and then used for protein blot with anti-GST and anti-T7 (EMD Millipore). For PR2 detection in S1 Fig ...
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bioRxiv - Synthetic Biology 2021Quote: ... Then equal amounts of total protein in each condition were resolved by SDS-PAGE and transferred to a PVDF membrane (Merck Millipore, ISEQ00010). The PVDF membrane was blocked with 1% (for anti-FLAG antibody ...
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bioRxiv - Microbiology 2020Quote: ... Immunoprecipitation of FLAG-tagged Hfq was performed by incubating each sample with 75 µl pre-washed (3 x resuspended in 1 ml lysis buffer and centrifuged at 10,000 rpm for 1 min) protein A sepharose beads (Merck/Sigma-Aldrich #P6649) for 45 min at 4 °C (rocking) ...
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bioRxiv - Microbiology 2020Quote: ... Equal amount of total protein was loaded and separated on 10% SDS-polyacrylamide gels and transferred to a polyvinylidene difluoride (PVDF) membranes (Millipore, IPVH00010). MAP kinase activation was detected by western-blot analysis with anti-phospho-p44/42 MAPK (Erk1/2 ...
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bioRxiv - Microbiology 2020Quote: ... FLAG- and HA-tagged proteins were detected by Western blotting using a 1:1000 dilution of primary anti-FLAG rabbit polyclonal antibody (Sigma, F7425) or anti-HA rabbit polyclonal antibody (Invitrogen ...
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bioRxiv - Systems Biology 2020Quote: ... Bait proteins were probed using mouse anti-FLAG antibody (Monoclonal anti-FLAG M2 antibody, Sigma-Aldrich, F3165; used at 1:2500) in blocking buffer ...
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bioRxiv - Systems Biology 2020Quote: ... Bait proteins from the supernatant were immuno-precipitated for 90 mins at 4°C with FLAG-M2 agarose beads (Millipore Sigma) and washed three times in the lysis buffer ...
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bioRxiv - Systems Biology 2020Quote: ... Acetone was discarded and protein pellets were air dried for 5-10 min and then resuspended in 100 μL of 50 mM TEABC (Sigma-Aldrich) Overnight enzymatic digestion was carried out using sequencing grade trypsin (Promega ...
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bioRxiv - Systems Biology 2020Quote: ... The equivalent of one hundred OD600 of lysed cells was used for the immunoprecipitation of the bait proteins using FLAG-M2 agarose beads (Millipore Sigma). The beads were incubated with the cell lysates for 120 mins at 4°C with slow rotation ...
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bioRxiv - Microbiology 2020Quote: ... and fractions containing target protein were collected and concentrated to 6-8 mg/ml using Amicon concentrators (Millipore, Billerica, MA, USA), flash-frozen with liquid nitrogen ...
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bioRxiv - Plant Biology 2021Quote: ... 17 µL of FLAG-tagged effector protein extract was added to the beads with 1 µL of 1 mM acetyl-CoA lithium salt (Sigma-Aldrich) and 2 µL of 1 µM IP6 (Sigma-Aldrich) ...
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bioRxiv - Plant Biology 2021Quote: ... Samples were washed three times with the protein elution buffer (20 mM Tris-HCl, 150 mM NaCl, 10% glycerol) and eluted by adding 3× FLAG peptide (Sigma-Aldrich) in 60 µL of the protein elution buffer following the manufacturer’s instruction.
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bioRxiv - Microbiology 2020Quote: ... The same amount of protein was injected into a 10% SDS-PAGE gel for separation and transferred to a polyvinylidene fluoride (PVDF) membrane (Millipore, Germany). Cell membranes were blocked at room temperature for 2 hours with 5% skim milk and incubated with primary antibodies overnight at 4°C ...
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bioRxiv - Neuroscience 2021Quote: ... 40μg of total proteins from each sample were separated by 8∼12% SDS-PAGE and transferred onto PVDF membranes (Millipore, USA), the membranes were blocked in 5% BSA ...
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bioRxiv - Microbiology 2021Quote: ... Pre-cleared lysate was diluted to 500 μl with additional lysis buffer and incubated overnight with 40 μl Protein A agarose beads and 4 μg anti-FcRγ-subunit IgG (Millipore Sigma) at 4°C on a Labquake Rotator ...
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bioRxiv - Neuroscience 2020Quote: ... Sections were then incubated for two nights at 4°C with primary antibodies against glial fibrillary acidic protein (GFAP; 1:5000; MAB360; Merck Millipore, USA), ionized calcium binding adaptor molecule 1 (Iba1 ...
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bioRxiv - Neuroscience 2020Quote: ... The proteins were transferred to a low fluorescent polyvinylidene difluoride membrane Immobilon-FL (0.45 μm pore size; Merck-Millipore, Czech Republic) by a Mini Trans-Blot® cell system (Bio-Rad ...
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bioRxiv - Microbiology 2021Quote: ... and stained with sACE2-humanFc fusion protein (construct is a gift of Jason McLellan, University of Texas at Austin) or mouse anti-Flag antibody (Sigma, F3165). Cells were then stained with anti-human-IgG-FITC (Sigma ...
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bioRxiv - Immunology 2021Quote: ... KLH protein was dissolved in PBS to 5 mg/mL of and then mixed 1:1 with CFA (both Sigma-Aldrich) upon which 40 μL of the immunization mixture was injected in footpads and flanks of draining popliteal and inguinal LNS ...
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bioRxiv - Microbiology 2021Quote: ... Lysates were adjusted to 1-2 mg total protein in 1 mL and incubated overnight at 4°C with 25 µL of packed Monoclonal Anti-HA Agarose (Sigma-Aldrich). Following incubation ...
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bioRxiv - Microbiology 2021Quote: ... The levels of reovirus μ1C/δ and the PSTAIR epitope of the host protein Cdk1 were determined by Western blot using an anti-reovirus primary antibody and an anti-PSTAIR primary antibody (Sigma-Aldrich), respectively.
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bioRxiv - Microbiology 2021Quote: ... The beads were washed 5 times in RIPA buffer and the immunoprecipitated proteins eluted using 150 μg/mL 3x Flag peptide (Sigma-Aldrich) in elution buffer (50 mM Tris/HCl pH 7.5 ...
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bioRxiv - Neuroscience 2019Quote: ... Equal amounts of protein (measured by BCA assay-Pierce) were used for free heme measurement following the manufacturer’s instructions (Sigma MAK-036).
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bioRxiv - Physiology 2021Quote: ... Trypsin was added at a ratio of 1:50 trypsin to protein (trypsin from porcine pancreas, Sigma Aldrich, catalog number T6567). Samples were incubated at 37°C overnight ...
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bioRxiv - Microbiology 2020Quote: ... Coverslips were next labeled as follows: HA-tagged proteins were detected by anti-HA rat monoclonal antibody 3F10 (Sigma, 1:200), parasite cyst wall and cyst matrix by in-house SalmonE anti-CST1 antibody (1:500 ...
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bioRxiv - Molecular Biology 2021Quote: ... of protein were separated by performing SDS-PAGE (10% or 12%) and then transferred onto polyvinylidene difluoride (PVDF) membranes (IPVH00005, Millipore, USA). Membranes were blocked for 1 hours with 5% skim milk at room temperature and then incubated overnight at 4 °C with the primary antibody ...
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bioRxiv - Cancer Biology 2020Quote: ... plate wells were washed with PBS containing 2% FBS and incubated overnight with biotinylated HA-binding protein (3 μg/ml, Calbiochem-EMD Millipore) at 4° C (16) ...
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bioRxiv - Cancer Biology 2020Quote: ... Equal volumes of protein samples were separated by 10% SDS/PAGE and electro-transferred to PVDF membranes (Millipore, Billerica, MA, USA). The immunoblots were probed with the indicated antibodies ...
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bioRxiv - Cell Biology 2021Quote: ... The resin was washed with 40 bed volumes of buffer A and the fusion protein was eluted with buffer A containing 5 mM d-Desthiobiotin (Sigma-Algrich). Peak atEH domain fractions were dialyzed overnight at 4°C against buffer A in the presence of TwinStrep-tagged TEV protease (32 ...
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bioRxiv - Cell Biology 2021Quote: ... The crude protein was separated by 12% SDS-PAGE and then transferred to a nitrocellulose filter membrane (Millipore, Billerica, MA, USA) using a Trans-Blot Semi-Dry transfer cell (Bio-Rad ...