Labshake search
Citations for Millipore Sigma :
9401 - 9450 of 10000+ citations for Recombinant Human ROR1 Protein His Avi tagged Biotinylated since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Cell Biology 2024Quote: ... 10-30 μg of protein was resolved by electrophoresis and then transferred to a polyvinylidene difluoride membrane (Millipore). Membranes were blocked in TRIS-buffered saline with 0.1% tween 20 and 5% BSA for 1 hour and then incubated overnight with primary antibody at a dilution of 1:1000 ...
-
bioRxiv - Cell Biology 2024Quote: ... Equal amounts of total protein were loaded onto an SDS-PAGE before transfer onto a nitrocellulose membrane (Millipore). The membranes were blocked in 5% (w/v ...
-
bioRxiv - Evolutionary Biology 2024Quote: ... Fractions containing the protein were pooled and concentrated with a 30 kDa cutoff Amicon centrifugal filter (Sigma UFC9030) and loaded onto a Superdex 200 size exclusion column equilibrated with the storage buffer (25mM HEPES pH 7.4 ...
-
bioRxiv - Genetics 2024Quote: ... cell surface protein samples were concentrated into 0.1 ml volumes using a 30 kDa centrifugal concentrator (Merck Millipore).
-
bioRxiv - Molecular Biology 2024Quote: ... For dTAG-13 induced protein degradation cells were treated with 500 nM dTAG-13 (Sigma-Aldrich, SML2601-5MG) or an equivalent volume of DMSO (Sigma-Aldrich ...
-
bioRxiv - Pathology 2024Quote: ... 0.5% Triton and lysates were incubated with rabbit or mouse isotypic control antibodies and G-protein beads (Sigma) for 1 hr at 4°C ...
-
bioRxiv - Bioengineering 2020Quote: ... co-staining was performed using rabbit anti-perilipin A antibody as an adipocyte marker in combination with a mouse anti-human mitochondria (hMito) antibody conjugated with Cy3 (EMD Millipore, Burlington, USA; 1:100 dilution). hMito was used as an alternative to Ku80 based on the availability of antibodies compatible for co-staining ...
-
bioRxiv - Cancer Biology 2021Quote: ... ERK5 was silenced with human or mouse shRNAs purchased as pre-cloned in the pLKO.1-puro plasmid (MISSION® shRNA, Sigma-Aldrich, St Louis, MO, USA). Control shRNAs in the same plasmid were also purchased (Sigma) ...
-
bioRxiv - Biochemistry 2021Quote: ... transferrin from human serum, (Tf), cytosine (Cyt), trifluoroacetic acid (TFA, ≥99%), and formic acid (FA, analytical grade >98%) were purchased from Sigma Aldrich (St. Louis, Missouri, United States). Methanol (MeOH) ...
-
bioRxiv - Immunology 2021Quote: ... secondary antibody (goat anti-human γ-chain-specific whole IgG, α-chain-specific IgA, or μ-chain-specific IgM alkaline phosphatase conjugate, Sigma Aldrich, MO, US) was diluted 1:1000 and 50μl was added per well for 1 hour ...
-
bioRxiv - Microbiology 2024Quote: ... the autoclaved peg lid was incubated in a flat-bottom 96-well plate with 200 µl of 100 µg/ml fibrinogen from human plasma (product number F3879, Sigma-Aldrich, in 0.9% NaCl or PBS) at 4°C overnight ...
-
bioRxiv - Molecular Biology 2023Quote: HEK293T cells were transfected as described above with empty or human SLFN14 expression plasmids and a plasmid encoding Hluc in the presence of RNA Pol III inhibitor (Sigma ML-60218, 40 and 20 uM) or tofacitinib (Sigma PZ0017 ...
-
bioRxiv - Immunology 2023Quote: ... Cytokine levels in matched BAL fluid and plasma collected from patients were measured using the multiplexed human cytokine/chemokine magnetic bead kits from Millipore (HCYTMAG-60K-PX41 and HCYP2MAG-62K) according to manufacturer’s protocol (HCYTOMAG-60K Rev ...
-
bioRxiv - Cancer Biology 2023Quote: ... and HPCD-3BP were incubated at various concentrations (12.5 μM, 25 μM, 50 μM, 100 μM, and 200 μM) with 90 μL of human serum (Sigma, Cat No. H3667; St. Louis, MO, USA) at 37 °C for up to 8 h ...
-
bioRxiv - Microbiology 2020Quote: ... Collected cells were immediately spun down and resuspended in BugBuster® Protein Extraction reagent (Novagen, Merck and Co. Inc) to lyse the bacterial cells and release proteins ...
-
bioRxiv - Cancer Biology 2021Quote: ... Proteins were separated by 10% SDS-PAGE gel and transferred to 0.45 µm polyvinylidene difluoride (PVDF) membrane (Millipore, USA). After blocking by with 5% non-fat dry milk for 2h at room temperature ...
-
bioRxiv - Molecular Biology 2020Quote: ... 25 μL of magnetic Protein G Dynabeads were coated with 2 μg of M2 anti-FLAG antibody (F1804, Sigma) for 1 hr at 4°C ...
-
bioRxiv - Cell Biology 2020Quote: Proteins were separated using sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) and transferred to polyvinylidene fluoride membranes (Millipore). After in-cubated with antibodies ...
-
bioRxiv - Cell Biology 2019Quote: Protein was extracted from isolated cell pellets or whole liver tissue using RIPA buffer with protease inhibitors (Sigma-Aldrich). Equal amounts of protein were loaded and separated by SDS-PAGE gel electrophoresis on 4%-20% Criterion gels (BioRad ...
-
bioRxiv - Cell Biology 2019Quote: ... the extracted proteins were separated in 10% polyacrylamide gels (Schägger and von Jagow, 1987) and blotted onto Immobilion-Fl membranes (Millipore). The membranes were incubated in blocking solution (5% skim milk in TBST ...
-
bioRxiv - Cell Biology 2019Quote: ... cells were serum starved for 48 hours and proteins were extracted with lysis buffer (Sigma Cell Lytic Solution C2978) with 0.1% CHAPSO ...
-
bioRxiv - Cell Biology 2020Quote: ... Total protein of cell extracts was determined by Bradford method and GST□BHMT was precipitated with glutathione agarose (Sigma). Precipitates were then mixed in SDS□PAGE sample buffer ...
-
bioRxiv - Cell Biology 2020Quote: ... Lamin A proteins were detected with mouse anti-myc antibodies (clone 4A6, Millipore cat #05-724; 1:10,000 dilution) decorated with goat anti-mouse secondary IRDye 680RD antibodies (LI-COR ...
-
bioRxiv - Microbiology 2020Quote: ... The mitochondrial import assay results were analysed by visualizing the proteins with the Coomassie stain (PhastGel Blue R, Sigma). The proteins were then fixed (20% ethanol and 10% acetic acid) ...
-
bioRxiv - Microbiology 2020Quote: ... 30 μg of total protein was analyzed by SDS-PAGE gel electrophoresis and transferred to a PVDF membrane (Millipore). The membrane was probed with antibodies at 4 °C overnight and visualized using Western Lightning™ Plus ECL (erkinElmer ...
-
bioRxiv - Molecular Biology 2021Quote: Western blot analysis of protein levels was performed on whole cell lysates prepared by lysis in RIPA buffer (Sigma) supplemented with 1 mM DTT (Sigma) ...
-
bioRxiv - Molecular Biology 2021Quote: ... The blots were incubated with primary antibodies to the following proteins overnight at 4 °C: (1) CTCF (1:1,000; 07-729, Merck Millipore), (2 ...
-
bioRxiv - Molecular Biology 2021Quote: ... Fractions containing Exo1 protein variant were pooled and applied to 0.3 mL of M2 anti-FLAG agarose beads (Sigma) in batch ...
-
bioRxiv - Developmental Biology 2021Quote: ... The lysate was centrifuged to remove cell debris and then incubated with Protein G Sepharose Fast Flow (Sigma-Aldrich) for 1 h at 4°C to remove nonspecifically bound proteins ...
-
bioRxiv - Genomics 2021Quote: ... Antibodies-chromatin complexes were then pulled-down using 20 μL Magna ChIP™ protein A+G magnetic beads (Millipore) by incubating with rotation (20 rpm ...
-
bioRxiv - Microbiology 2021Quote: ... Sized proteins were brought to concentrations typically >50 mg mL−1 using appropriate molecular weight cut-off concentrators (Millipore), flash frozen on liquid nitrogen ...
-
bioRxiv - Developmental Biology 2021Quote: ... 20 ug to 200 ug of protein was run on a 7.5 or 10% SDS-PAGE gel and transferred onto a PVDF membrane (Sigma-Millipore). The membranes were blotted for 1 hr in 1 ...
-
bioRxiv - Cancer Biology 2022Quote: ... and 30µg to 50µg of total proteins were loaded onto 7.5% or 12% SDS-polyacrylamide gels for electrophoresis and transferred onto polyvinylidene difluoride membranes (Millipore). After 1h of blocking with 5% bovine serum albumin or non-fat milk prepared in Phosphate-Buffered Saline (PBS)-0.1% Tween-20 buffer ...
-
bioRxiv - Evolutionary Biology 2022Quote: ... The fluorescent proteins encoded on the plasmid also carries a gene for antibiotic resistance (Gentamicin 418, Sigma-Aldrich: G418). Cells were cultured in autoclaved HL5 medium (per L ...
-
bioRxiv - Genetics 2022Quote: ... 25 μg chromatin was diluted with IP dilution buffer and pre-cleared with Protein A agarose beads (Merck-Millipore) for 1 h at 4°C ...
-
bioRxiv - Neuroscience 2021Quote: Sections were washed in 0.01M PBS and then placed in primary antibody (NeuN monoclonal mouse anti-neuronal nuclear protein, Millipore MAB377 ...
-
bioRxiv - Neuroscience 2021Quote: ... and performed western blotting analysis.Western blotting: Protein concentration of lysates was determined with Bicinchoninic Acid Kit (#BCA1, Sigma Aldrich). Equal amount of proteins was loaded on the poly-acrylamide gels ...
-
bioRxiv - Molecular Biology 2021Quote: The protein concentrations of the lysates were determined by an amido black filtration assay56 with Fraction V BSA (Sigma) for a standard curve ...
-
bioRxiv - Molecular Biology 2020Quote: ... A 20 μl sample of the cleared protein was mixed with 10 μl of UPS2 spike-in (Sigma-Aldrich) and diluted in 250 μl 1.25× protein loading buffer ...
-
bioRxiv - Molecular Biology 2021Quote: ... Unbound fiber knob protein was removed by washing twice in cold PBS and primary CAR RmcB (Millipore; 05-644) or primary CD46 MEM-258 (ThermoFisher ...
-
bioRxiv - Molecular Biology 2021Quote: ... The protein was eluted in 7 aliquots of 700 μl each with elution buffer (10 mM reduced glutathione (Sigma 4251-5G in 50 mM Tris-HCl ...
-
bioRxiv - Molecular Biology 2020Quote: ... The appropriate horseradish peroxidase-coupled secondary antibodies were used to reveal the proteins (antimouse at 1:10,000 (Sigma, A2554) and antirabbit at 1 ...
-
bioRxiv - Cell Biology 2022Quote: ... were added for 1 hour at room temperature and protein signal was then visualized using Immobilon Forte (Millipore, WBLUF0500) on ImageQuant LAS 4000 or Amersham ImageQuant 800 ...
-
bioRxiv - Immunology 2022Quote: ... Both multivalent proteins were buffer exchanged to remove excess imidazole and concentrated with 10 kDa cutoff centrifuge filters (Millipore), then filter sterilized by 22 μm centrifugal sterile filter (Millipore Sigma ...
-
bioRxiv - Immunology 2021Quote: ... Protein lysates (25 μg) were resolved by SDS-PAGE and transferred to Immobilon-P PVDF membrane (Sigma-Aldrich, IPVH00010). Membranes were blocked in 5% nonfat milk in TBST buffer at room temperature for 1 hour ...
-
bioRxiv - Molecular Biology 2021Quote: ... Proteins were separated on an SDS-PAGE gel and then transferred to a nitrocellulose (NC) membrane (Merck Millipore, ISEQ00010), which was incubated with individual protein-specific primary antibodies at 4 °C overnight on a shaker ...
-
bioRxiv - Biophysics 2019Quote: ... lyophilized protein was dissolved in sodium phosphate buffer (pH 7.4) and filtered using 0.22 μm low protein binding membranes (Millex-GP, Merck Millipore, Germany). The protein concentration was determined by the measurement of absorbance at 277 nm using the extinction coefficient 5960 cm-1M-1.
-
Glyoxal Does Not Preserve Cellular Proteins as Accurately as PFA: A Microscopical Survey of EpitopesbioRxiv - Cell Biology 2019Quote: ... Then slides were incubated with antibodies specific to acrosin binding protein (ACRPB) (1:100 in PBS) (Sigma; HPA039081, USA), IZUMO1 (1:100 in PBS ...
-
CASC3 promotes transcriptome-wide activation of nonsense-mediated decay by the exon junction complexbioRxiv - Molecular Biology 2020Quote: ... 600 µl of a 1.6 mg/ml total protein lysate were incubated with 30 µl Anti-FLAG M2 magnetic beads (Sigma) at 4°C while rotating for 2 h ...
-
bioRxiv - Molecular Biology 2019Quote: Proteins were separated on a SDS-PAGE gel at an acrylamide percentage appropriate to protein size and transferred onto a polyvinylidene difluoride membrane (PVDF, 0.45 μm Millipore). The membrane was blocked in 5 % milk in PBS 0.1 % Tween 20 (PBS-T ...