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Citations for Millipore Sigma :
9451 - 9500 of 10000+ citations for Recombinant Human ROR1 Protein His Avi tagged Biotinylated since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biophysics 2019Quote: ... after which the protein-bound stain was dissolved in 150 µl of 10mM Tris-base (Sigma-Aldrich, Zwijndrecht, Netherlands). The optical density was read at 540 nm with the HTX Synergy microplate reader (BioTek ...
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bioRxiv - Neuroscience 2019Quote: A total mass of 600μg brain protein was subjected to reduction with 10mM Tris(2-carboxyethyl)phosphine(TCEP, Sigma) at 30°C for 2hrs and then alkylation with 20mM IAA containing 100mM ABB in the dark at room temperature for 30mins ...
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bioRxiv - Developmental Biology 2019Quote: ... into single-cell suspension and re-suspended in E8 media containing 10 μM Rho-associated protein kinase inhibitor (Sigma). Approximately 100,000 cells were re-plated into Matrigel-coated 6-well plates ...
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ARID1a protects against steatosis and insulin resistance via PPARalpha-mediated fatty acid oxidationbioRxiv - Cell Biology 2019Quote: ... each protein sample (50 mg) was subjected to SDS/PAGE and transferred to NC membrane (Millipore, Bedford, MA, USA). Then the corresponding primary and secondary antibodies were incubated to visualize the protein ...
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bioRxiv - Microbiology 2019Quote: ... The protein band was excised from gel and digested with trypsin using trypsin profile IGD kit (Sigma-Aldrich, India). The peptides obtained after trypsin digestion were analyzed by Matrix-assisted Laser Desorption Ionization-Time of Flight (MALDI-TOF ...
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bioRxiv - Biochemistry 2019Quote: ... Protein was transferred onto a PVDF membrane (Immobilon-P) and detected using mouse anti-c-Myc antibody (Millipore Sigma) as a primary (1:5,000 dilution ...
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bioRxiv - Cell Biology 2019Quote: ... 20 μg of proteins were separated by SDS-PAGE and transferred to a nitrocellulose membrane (Millipore, Billerica, MA, USA). Membranes were blocked with 0.1% Tween 20 and 5% BSA for 1 h before overnight incubation with the primary antibody diluted in blocking solution ...
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bioRxiv - Immunology 2019Quote: ... chromatin was precleared by rotating for 2 h at 4°C with 100 μl of 50% protein A/G slurry (0.2 mg/ml sheared salmon sperm DNA, 0.5 mg/ml BSA, and 50% protein A/G; Sigma-Aldrich), 0.3−0.5×106 cell equivalents were saved as input ...
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bioRxiv - Biochemistry 2019Quote: Protein samples of in vitro translation reactions and subsequent purifications were separated on SDS-PAGE at neutral pH condition (pH 6.8, for purified protein samples) and were transferred on PVDF membrane (Immobilon-P, Millipore). After blocking with 5% skim milk in PBS-T ...
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bioRxiv - Neuroscience 2019Quote: ... proteins were transferred electrophoretically to polyvinylidene difluoride membranes and probed with the following antibodies:anti-EphB6 (1:500, SAB4503476, Sigma), anti-EphB1 (1:500 ...
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bioRxiv - Physiology 2020Quote: ... Proteins were transferred onto nitrocellulose membranes and immunoblot analyses were carried out using rabbit antibodies directed against ShcA (Millipore), Collagen X (Abcam) ...
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bioRxiv - Neuroscience 2019Quote: ... 450-1000 µg of total protein was immunoprecipitated overnight with 20 µl (50% slurry) of anti-FLAG (Sigma, F2426) affinity gel at 4°C on a nutator ...
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bioRxiv - Molecular Biology 2019Quote: ... Bound proteins were cleaved off the column with thrombin diluted 1:250 in thrombin cleavage buffer (Novagen #69671-3). The thrombin solution was loaded manually into the column with a syringe ...
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bioRxiv - Pharmacology and Toxicology 2019Quote: ... the immuno cytokine was radio-iodinated with 125I (Hartmann Analytic) and Chloramine T hydrate (0.25 μg/μg protein, Sigma) and purified on a PD10 column (GE Healthcare) ...
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bioRxiv - Cell Biology 2019Quote: ... Oligomeric α-synuclein species were removed by filtering the protein through a 50-kDa cut-off column (Amicon, Millipore). Aliquots of the protein were prepared ...
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bioRxiv - Neuroscience 2019Quote: ... The mixture was then incubated with 20 μL of 50% protein G plus-agarose (Sigma-Aldrich, St. Louis, MO) for 4 h at 4°C with gentle rocking ...
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bioRxiv - Cancer Biology 2019Quote: ... ChIP dilution buffer was used to dilute the sheared chromatin followed by preclearing with Protein G Agarose beads (Sigma) for 30min ...
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bioRxiv - Cell Biology 2019Quote: ... the proteins were blotted onto a nitrocellulose membrane and probed with anti-GFP (1:1000; Sigma-Aldrich and Roche), anti-dgt6 (1:500 ...
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bioRxiv - Immunology 2019Quote: ... the proteins samples were incubated with 50 μg/mL of polymyxin B sulphate salt (Sigma-Aldrich, St. Louis, MO) for 30 min at 37 °C to remove possible residual LPS.
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bioRxiv - Biochemistry 2019Quote: ... Proteins were resuspended in a solution containing 1 % SDC and 10 mM TCEP with 40 mM CAA (Sigma-Aldrich) as reduction and alkylating agents and heated to 37 °C for 1 hour ...
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bioRxiv - Immunology 2020Quote: ... Proteins were precipitated overnight at −20°C with six volumes of pre-chilled (−20°C) acetone (Sigma-Aldrich, USA). The samples were centrifuged at 8,000xg for 10 minutes at 4°C to obtain the protein pellets and supernatants were discarded ...
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bioRxiv - Immunology 2020Quote: ... Proteins were precipitated overnight at −20°C with six volumes of pre-chilled (−20°C) acetone (Sigma-Aldrich, USA). The samples were centrifuged at 8,000xg for 10 minutes at 4°C to obtain the protein pellets and supernatants were discarded ...
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bioRxiv - Physiology 2020Quote: ... Proteins were resolved on a 4%–12% SDS-PAGE gel and transferred to a PVDF membrane (Immobilon-FL, Millipore), incubated with indicated antibodies overnight at 4°C and subsequently quantitated with the LiCOR Odyssey instrument with Odyssey Image Studio software using 2 different infrared channels ...
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bioRxiv - Immunology 2019Quote: ... 20 µg to 50 µg of protein for each sample was mixed with 2X Laemmli sample buffer (S3401, Sigma) and heated at 50°C for 10 minutes ...
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bioRxiv - Animal Behavior and Cognition 2019Quote: Eight µg of IrSPI were refolded for 30 minutes at 25°C in a final volume of 100µl containing 10µg of the disulfide isomerase protein (Sigma-Aldrich) with reduced/oxidised glutathione (Sigma-Aldrich ...
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bioRxiv - Biophysics 2021Quote: ... were used at 1,000 × g and 4 °C to exchange purified protein samples to 250 mM ammonium acetate (99.99 % purity; Sigma-Aldrich), pH 8.0 as buffer surrogate ...
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bioRxiv - Cell Biology 2021Quote: ... Insoluble remnants were removed by centrifugation at 16,000 g for 5 min and 140 μg clarified protein extract was transferred to a 30 kDa MW cut-off spin filter (Amicon Ultra 0.5mL Filter, Millipore). SDS was completely replaced by repeated washing with 8 M urea ...
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bioRxiv - Immunology 2021Quote: Mice were sensitized on day 0 by intraperitoneal injection of 50 μg lyophilized BSA-free ovalbumin protein extract (Sigma) resuspended in PBS and pre-incubated with the adjuvant Imject Alum (Thermo) ...
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bioRxiv - Microbiology 2021Quote: ... 1.5×105 Vero cells in 24-well plates were transfected with 0.5 µg of the indicated S-protein expression vector using the GeneJuice transfection reagent (Novagen) and syncytia formation monitored for 24-48 hours post-transfection ...
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bioRxiv - Neuroscience 2021Quote: ... Bottles were filled with either protein-containing solution (4% casein, sodium salt from bovine milk, Sigma #C8654; 0.21% L-methionine, Sigma #M9625 ...
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bioRxiv - Biochemistry 2021Quote: ... Peak fractions corresponding to soluble protein were pooled and concentrated to 4.5–6.5 mg/mL (Amicon Ultra 100 kDa cut off, Millipore Sigma). Protein samples were flash-frozen in liquid nitrogen and stored at −80°C.
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bioRxiv - Biochemistry 2021Quote: ... Peak fractions corresponding to soluble protein were pooled and concentrated to 4.5–5.5 mg/mL (Amicon Ultra 100 kDa cut off, Millipore Sigma). Protein samples were flash-frozen in liquid nitrogen and stored at −80°C.
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bioRxiv - Biochemistry 2020Quote: ... The protein was concentrated to 40 mg ml-1 with a centrifugal filter device (Millipore 50 kDa MW cutoff), and frozen until crystallization.
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bioRxiv - Biochemistry 2020Quote: ... The eluted protein was concentrated and buffer exchanged into 0.5 x TNG buffer using an Amicon ultrafiltration device (Millipore) containing a 10 kDa membrane.
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Tracing production instability in a clonally-derived CHO cell line using single cell transcriptomicsbioRxiv - Cell Biology 2021Quote: ... supernatant collected at 240 hrs was filtered with 0.45 µm and 0.2 µm low-binding protein Durapore® filters (Merck Millipore). 25 µL of clarified supernatant was directly injected on a Vanquish™ uHPLC (Thermo Sceintific ...
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bioRxiv - Bioengineering 2020Quote: ... single-polymer and IPN hydrogels were incubated with an HA binding protein derived from bovine nasal cartilage (EMD Millipore) as previously described by Suri & Schmidt [31] ...
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bioRxiv - Biochemistry 2021Quote: ... The rAQP4ct protein domain was concentrated and exchanged into buffers for biophysical analyses with an Amicon centrifugal filter (Millipore).
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bioRxiv - Biochemistry 2020Quote: ... The protein sample was concentrated by ultrafiltration (Amicon Ultra-4 centrifugal filter devices, 50,000 MWCO; Millipore, Billerica, MA, USA), and the solution was changed to buffer A ...
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bioRxiv - Biochemistry 2020Quote: ... Portions of 10 g cell pellet were resuspended in 50 mL Bugbuster (BugBuster® Protein Extraction Reagent, Merck Millipore) supplemented with 5 µL Benzonase Nuclease (Novagen ...
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bioRxiv - Biochemistry 2020Quote: ... The bacterial pellet was suspended in 5 mL lysis buffer (BugBuster Protein Extraction Reagent, Merck Millipore, UK; containing DNAse) and incubated at room temperature for 30 mins ...
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bioRxiv - Biochemistry 2020Quote: ... The bacterial pellet was suspended in 5 mL lysis buffer (BugBuster Protein Extraction Reagent, Merck Millipore, UK; containing DNAse) and incubated at room temperature for 30 mins ...
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bioRxiv - Biochemistry 2020Quote: Proteins separated on PAGE gel were transferred to a PVDF membrane (Immobilon-P/Immobilon-FL, 0.45 μm, Merck Millipore) using Trans-Blot® Turbo™ Transfer System (Bio-Rad) ...
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bioRxiv - Biochemistry 2020Quote: ... Solubilized total proteins were separated by SDS-PAGE and electro-blotted onto a PVDF membrane (Merck Millipore; Billerica, MA). The membrane was first blocked with 5% skim milk in PBST (Phosphate Buffered Saline with Tween 20) ...
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bioRxiv - Biochemistry 2020Quote: ... The protein was then labeled with the MDCC (7-Diethylamino-3-[N-(2-maleimidoethyl) carbamoyl]coumarin) from Sigma-Aldrich. Unreacted MDCC was removed by ion exchange using a Bio-Rex70 column ...
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bioRxiv - Neuroscience 2021Quote: ... the cells were incubated with the primary antibodies of interested proteins at 4°C overnight (Nestin, 1:1000, Sigma; Tuj1 ...
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bioRxiv - Neuroscience 2020Quote: ... Proteins were reduced by adding 5 mM DTT (45 min, 56 °C) and free cysteines alkylated with iodoacetamide (Sigma, Taufkirchen ...
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bioRxiv - Neuroscience 2020Quote: ... The rest was incubated with 30μl of Protein G Dynabeads and 3 μl of anti-FLAG M2 antibody (Sigma) overnight to immunoprecipitate (IP ...
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bioRxiv - Cell Biology 2021Quote: ... and the expression of the bait-protein was detected from the input sample with anti-FLAG antibody (Sigma Aldrich).
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bioRxiv - Cell Biology 2021Quote: ... The protein complexes associated with p53 were resolved by SDS-PAGE and transferred onto a PVDF membrane (#IPVH00010, Millipore). The membrane was blocked with 3% BSA in PBS for 1 h at room temperature ...
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bioRxiv - Cancer Biology 2020Quote: ... Proteins were eluted twice for 30 minutes each at 58°C with 150 mM dithiothreitol (DTT; D0632, Sigma-Aldrich), 1% SDS ...