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Citations for Millipore Sigma :
9301 - 9350 of 10000+ citations for Recombinant Human ROR1 Protein His Avi tagged Biotinylated since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2024Quote: ... Each reaction mixture constituted protein at the desired concentration with 1.25 mM Tris (2,2’-bipyridyl)dichlororuthenium(II)hexahydrate (Sigma, CAS No ...
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bioRxiv - Neuroscience 2024Quote: 10 µg of protein was loaded per sample and membrane was probed with antibodies against APOE (Sigma; AB947), RHEB (Abcam ...
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bioRxiv - Biochemistry 2024Quote: ... appropriate AGO:RNA bands were excised from the gel and protein digested with Proteinase K (RPROTK-RO, Sigma Aldrich) and released RNA isolated via phenol:chloroform phase separation ...
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“Identification of microRNAs regulated by E2F transcription factors in human pluripotent stem cells”bioRxiv - Developmental Biology 2024Quote: ... Equal amounts of protein were electrophoresed on a 12% SDS– polyacrylamide gel and transferred to PVDF membranes (Millipore). Blots were blocked for 1 hour at RT (room temperature ...
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bioRxiv - Biochemistry 2023Quote: ... was incubated for 2 hrs at 4 °C with Protein A/G Mix Magnetic Beads (Cat# LSKMAGAG10, Millipore). After washing with dilution buffer ...
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bioRxiv - Cell Biology 2024Quote: Protein samples were separated by 10-12% SDS-PAGE and electrotransferred to nitrocellulose membranes (Merck Millipore, Cat # ISEQ00010). Immunoblotting was carried out as follows ...
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bioRxiv - Immunology 2023Quote: ... The eluted protein was concentrated to 500 μl with a 100-kDa molecular mass cut-off concentrator (Millipore). Concentrated apo-KSHV-GPCR-Gi complex was loaded onto a Superdex 200 Increase 10/300 GL column (GE Healthcare ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... Protein quantification for each sample was conducted by BCA Assay in accordance with kit instructions (Millipore Sigma 71285M).
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bioRxiv - Biochemistry 2024Quote: ... Protein was concentrated to 10 mL using an Amicon Ultra-15 10K MWCO Centrifugal Filter Unit (Merck Millipore) and loaded into SnakeSkin Dialysis Tubing ...
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bioRxiv - Biophysics 2024Quote: ... The instrument was calibrated using a protein standard mixture: β-amylase (Sigma-Aldrich, 56, 112 and 224 kDa), and thyroglobulin (Sigma-Aldrich ...
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bioRxiv - Biochemistry 2024Quote: ... Proteins were separated by SDS-polyacrylamide gel electrophoresis (PAGE) electroblotted onto a polyvinylidene difluoride (PVDF) membrane (Millipore, IPFL00010). PVDF membrane was masked with Intercept Blocking Buffer (LI-COR ...
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bioRxiv - Developmental Biology 2023Quote: ... The proteins bound to the beads were reduced with 10 mM Tris(2-carboxyethyl)phosphine hydrochloride (Sigma, C4706) at 40°C for 30 min and alkylated with 20 mM Iodoacetamide (Sigma ...
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bioRxiv - Cell Biology 2023Quote: ... The fractions corresponding to the protein complexes were concentrated using a 500-μl 30K MWCO concentrator (Millipore-Sigma).
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bioRxiv - Cell Biology 2023Quote: ... The fractions corresponding to the protein complexes were concentrated using a 500-μl 30K MWCO concentrator (Millipore-Sigma).
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bioRxiv - Cancer Biology 2022Quote: ... Ras protein level in the pull down was analyzed by by immunoblotting using RAS antibody (Sigma-Aldrich #OP40). Total was lysate was also subjected to immunoblotting to determine RAS expression level as the input.
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bioRxiv - Molecular Biology 2023Quote: ... An equivalent amount of protein lysate was subjected to SDS-PAGE and immunoblotting with antibodies against FLAG (Sigma), V5 (Cell Signaling ...
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bioRxiv - Microbiology 2023Quote: ... Protein was detected using HRP-conjugated anti-mouse secondary antibody (Amersham) and Luminata Forte Western HRP substrate (Millipore).
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bioRxiv - Microbiology 2023Quote: ... The remaining RNA was combined with 25 μl of Magna ChIP Protein A+G magnetic beads (Merck Millipore) coated in 5μl of anti-m6A antibody (Merck Millipore ...
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bioRxiv - Neuroscience 2023Quote: ... 30-100 μg of protein were resolved by SDS-PAGE and transferred to nitrocellulose membranes (Protran, Sigma-Aldrich). Membranes were blocked in PBS or TBS containing 3% BSA or 5% skimmed milk ...
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bioRxiv - Developmental Biology 2022Quote: ... Purified protein was dialysed against water then concentrated using an Amicon Ultra-Ultracel 5 kDa centrifugal filter (Millipore). Antibodies were raised in two guinea pigs by Eurogentec.
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bioRxiv - Systems Biology 2023Quote: ... Proteins were then separated on 12% SDS PAGE and transferred to a PVDF membrane (Millipore Sigma, 0.2 μM). Membranes blocked with 5% milk were then probed with anti-HA (1:2000 ...
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bioRxiv - Cell Biology 2023Quote: ... Fractions containing desired protein were pooled and concentrated to 500 μL by ultrafiltration (Amicon Ultra-15, EMD Millipore), then passed over a size exclusion column (HiLoad Superdex 200 PG ...
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bioRxiv - Immunology 2023Quote: ... Proteins were then precipitated by addition of 800 µL of ice-cold 55% trichloroacetic acid (TCA) (Sigma Aldrich). Samples were left on ice for 15 minutes ...
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bioRxiv - Cell Biology 2023Quote: ... 30 µg of total protein extracts were separated on acrylamide-SDS gels and transferred to PVDF membranes (Millipore). After the nonspecific binding sites had been blocked ...
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bioRxiv - Microbiology 2023Quote: ... Fractions containing purified protein were united and concentrated on Amicon-Ultra-15 10 K filtration units (Merck-Millipore, Darmstadt ...
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bioRxiv - Evolutionary Biology 2023Quote: ... Sized proteins were concentrated to >50 mg mL-1 using 10 kDa molecular weight cut-off concentrators (Millipore), flash frozen on liquid nitrogen ...
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bioRxiv - Systems Biology 2023Quote: Well plates containing single-cell protein lysates were digested with 2 ng of Trypsin (Sigma cat. nr. T6567) supplied in 1 μL of digestion buffer (100 mM TEAB pH 8.5) ...
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bioRxiv - Cell Biology 2023Quote: ... Protein lysates were resolved using 10% SDS-PAGE gels and transferred to 0.45 µm PVDF membranes (Millipore, IPVH00010). The membranes were blocked in 5% skim milk solution for 1 h at RT then incubated with primary antibody at 4 ℃ overnight ...
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bioRxiv - Genetics 2023Quote: ... Immunoprecipitation was performed on total-protein lysate using 1 μg of rabbit polyclonal TTC29 antibody (HPA061473, Sigma-Aldrich) and 15 μl of Protein A/G magnetics beads (Bio-Ademtec ...
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bioRxiv - Developmental Biology 2023Quote: Proteins were separated on denaturing polyacramide gels before transfer to a 0.45 μm Immobilon-P PVDF membrane (Millipore) in transfer buffer (25 mM Tris ...
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bioRxiv - Developmental Biology 2023Quote: ... The following primary antibodies were used for protein blotting: mouse anti-c-Myc antibody (Sigma-Aldrich, 05-724MG), mouse anti-Flag antibody (Sigma-Aldrich ...
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bioRxiv - Microbiology 2023Quote: ... the eluted proteins were concentrated in an Amicon®Ultra-15 Centrifugal Filter Unit (Millipore; 10 kDa cutoff) and incubated with the SUMO protease Ulp1 (Ulp1:His-SUMO-tH2 = 1:500 w/w ...
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bioRxiv - Microbiology 2023Quote: ... and cells were probed using primary antibodies to VZV proteins IE62 (mouse mAb; EMD Millipore MAB8616; 1:200), capsid-ORF23 (polyclonal rabbit ...
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bioRxiv - Molecular Biology 2023Quote: ... Proteins were extracted from 400,000 to 500,000 cells with RIPA buffer with the addition of proteinase inhibitor (Sigma) and phosphatase inhibitors (Sigma) ...
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bioRxiv - Neuroscience 2023Quote: ... The primary antibodies used were anti-glial fibrillary acidic protein (GFAP) antibody (dilution; 1:100, Sigma-Aldrich (G6171), St ...
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bioRxiv - Cell Biology 2023Quote: ... equal amounts of protein were subjected to SDS polyacrylamide gel electrophoresis and transferred to nitrocellulose membrane (Millipore Sigma) by semi-dry transfer ...
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bioRxiv - Cell Biology 2023Quote: ... Spastin protein was resolved on 8% SDS-PAGE gels transferred to Immobilon®-FL PVDF membranes (Millipore; 05317) using a wet blot transfer system (BioRad) ...
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bioRxiv - Cell Biology 2023Quote: ... equal amounts of protein were subjected to SDS polyacrylamide gel electrophoresis and transferred to nitrocellulose membrane (Millipore Sigma) by semi-dry transfer ...
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bioRxiv - Biochemistry 2023Quote: mAb TnI-1 (IgG1) was purified from mouse hybridoma ascites fluid using Protein G chromatography (Millipore Sigma P7700). TnI-1 IgG eluted with 50 mM glycine-HCl ...
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bioRxiv - Biochemistry 2022Quote: ... The expression of the proteins was assessed by western blot using antibodies raised against the T7 epitope (Novagen) and GAPDH (Abcam).
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bioRxiv - Biochemistry 2023Quote: ... The peak fractions containing the protein were pooled and concentrated in centrifugal concentrators (CLVG01230, Merck Millipore, Cork, Ireland) to a final concentration of 30 mg/ml for crystallization ...
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bioRxiv - Biophysics 2023Quote: ... Protein degradation was induced by treating cells with a final concentration of 400 µM dTAG-13 (Sigma SML2601) for 2 hrs or 6 hrs (2 hrs ...
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bioRxiv - Cell Biology 2022Quote: ... N-glycosylated proteins were enriched by incubating cell lysates containing protease inhibitor and 1,10-phenanthroline (Sigma-Aldrich, #131377) with 20µl concanavalin A Sepharose beads (Sigma-Aldrich ...
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bioRxiv - Cell Biology 2023Quote: ... HIV-Tat protein transduction motif (YGRKKRRQRRR) was subcloned into a pET-46 Ek/LIC vector (EMD Millipore #71335). The construct encoding a GST-tagged caltubin was subcloned into a pGEX-4T-1 vector (Amersham #28954549) ...
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bioRxiv - Developmental Biology 2023Quote: ... Proteins were separated on GenScript SurePAGETM Bis-Tris gels and blotted on the Immobilon-P transfer membrane (Millipore). The membrane was blocked with 5% skim milk and incubated with primary antibodies ...
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bioRxiv - Developmental Biology 2023Quote: ... Cells were then incubated for 15 minutes in 2% BSA with globular actin-binding protein GC globulin (Sigma). Following incubation ...
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bioRxiv - Bioengineering 2023Quote: ... Proteins were run on NuPAGE 4%–12% Bis-Tris Gel (Novex) and transferred onto PVDF membranes (Millipore Sigma) and probed with antibodies ...
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bioRxiv - Neuroscience 2023Quote: ... Homogenate of 50 μg was added to a process control of 800 ng of yeast enolase protein (Sigma) which was then reduced with 20 mM DTT and alkylated with 40 mM IAA ...
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bioRxiv - Microbiology 2023Quote: ... Proteins were filter-concentrated using centrifugation and a 10-kDa or 30-kDa cut-off column (Millipore Sigma) and dialyzed at 4 °C for ∼24 h against Dialysis Buffer (50 mM Tris-HCl pH 7.5 ...
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bioRxiv - Cell Biology 2023Quote: ... Samples were pre-cleared for 1 h with protein A Sepharose CL-4B (Sigma-Aldrich, 17-0780-01) beads blocked with 0.2 µg/µl sonicated herring sperm DNA (Thermo Fisher ...