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Citations for Millipore Sigma :
9501 - 9550 of 10000+ citations for Recombinant Human ROR1 Protein His Avi tagged Biotinylated since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2021Quote: ... membranes are blocked with 5% milk or BSA for phosphorylated proteins in TBS+0.2% Tween®20 (Sigma-Aldrich) for 1h and incubated 1h with primary antibody at room temperature ...
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bioRxiv - Neuroscience 2020Quote: ... Values were normalized with respect to the total protein on each sample determined by the BCA method (Sigma-Aldrich).
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bioRxiv - Neuroscience 2019Quote: ... The protein sample was incubated with taxol-stabilized microtubules (15 mg mL-1) and 1 mM AMP-PNP (Sigma) and the mixture overlaid on a glycerol cushion (BRB80 ...
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bioRxiv - Plant Biology 2021Quote: ... The cultured cells were collected by centrifugation (8,000 × g for 10 min at 4 °C) and suspended in BugBuster Protein Extraction Reagent according to the manufacturer’s procedures (Merck Millipore). The supernatant was collected by centrifugation (12,000 × g for 10 min at 4 °C) ...
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bioRxiv - Biochemistry 2020Quote: ... Protein post cleavage was injected into FPLC and run over two HisTrap FF Crude 1 mL columns (Sigma-Aldrich) with FPLC running buffer ...
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bioRxiv - Biochemistry 2020Quote: ... NaCl was removed from the buffer and the protein was concentrated to 100 mg mL−1 using a 50,000 Da molecular mass cut-off concentrator (Millipore). The concentrated protein was incubated with a four-fold molar excess of dalbavancin at 4 °C for 12 h ...
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bioRxiv - Neuroscience 2019Quote: ... 150 μg of protein from each sample were loaded onto a 10-kDa Amicon Ultra-0.5 centrifugal filter (Millipore). The protein mixture was washed three times by adding 200 μL of UA buffer (8 M Urea ...
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bioRxiv - Cell Biology 2021Quote: ... Fractions containing the S protein were pooled and concentrated using 50 kDa MWCO Amicon centrifugal filter units (EMD Millipore). During concentration ...
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bioRxiv - Plant Biology 2021Quote: ... Total proteins were extracted for measuring the activity of GUS and luciferase (LUC) activity using 4-methylumbelliferyl glucuronide (Sigma) and luciferin (Promega ...
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bioRxiv - Microbiology 2020Quote: ... Proteins were separated via gel electrophoresis on 7.5-12 % polyacrylamide gel and transferred to a PVDF membrane (Sigma Aldrich) using a semi-dry blotting system ...
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bioRxiv - Microbiology 2020Quote: ... the proteins in another gel were electrophoretically transferred to a methanol-activated polyvinylidene difluoride (PVDF) membrane (Millipore, Billerica, MA) and reacted with COVID-19 hospitalized patient sera (#12) ...
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bioRxiv - Microbiology 2020Quote: ... cell pellets were lysed with the M-PER Protein Extraction Reagent (Pierce) complemented with a protease inhibitor cocktail (Sigma) and a phosphatase inhibitor cocktail (Sigma) ...
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bioRxiv - Plant Biology 2020Quote: ... The protein extraction buffer (50 mM TrisHCl, pH 7.0, 1% w/v SDS, P9599 protease inhibitor cocktail, Sigma-Aldrich) was added to the powder ...
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bioRxiv - Synthetic Biology 2021Quote: ... Ll.LtrB intron and LtrA protein was amplified from the commercial plasmid pACD4K-C (TargeTron gene knockout system, Sigma-Aldrich) with primers pGIIintron_fwd and rev (Supplementary Table S1) ...
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bioRxiv - Plant Biology 2020Quote: ... 200 mg of tissue was ground in liquid nitrogen and protein was precipitated by 10% TCA (Trichloroacetic acid, Sigma) in acetone and the resultant precipitate was pelleted by centrifuging at 13000 rpm for 5 min ...
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bioRxiv - Neuroscience 2021Quote: ... Viral supernatant was transferred into a new sterile 50 mL tube and passed through a low protein binding 0.45 μm filter (SE1M003M00, Millipore) to exclude any cells still present in the supernatant ...
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bioRxiv - Microbiology 2021Quote: ... 12 μg of protein was resolved by SDS-PAGE on a 17.5% acrylamide gel and transferred to an Immobilon-P membrane (Millipore). The membranes were blocked with 5% bovine serum albumin in 1XPBS with 0.5% Tween 20 (PBST ...
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bioRxiv - Microbiology 2021Quote: ... 50 µg of purified Cu-LpmP or CslZ protein were incubated for 3 h at room temperature with 0.2% peptidoglycan (PG) from Bacillus subtilis (Sigma), 5 mg α-chitin from shrimp shells (Sigma ...
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bioRxiv - Microbiology 2021Quote: ... Proteins were separated by 12% SDS-polyacrylamide gel electrophoresis (PAGE) and transferred by electroblotting onto polyvinylidene difluoride membrane (Millipore). After blocking 1 h with 5% nonfat dry milk ...
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bioRxiv - Microbiology 2021Quote: ... The proteins were reduced and alkylated after treatment with 10 mM DTT (Astral Scientific) and 20 mM chloroacetamide (Sigma). The gel was dehydrated using 100% ACN and rehydrated with digestion solution ...
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bioRxiv - Microbiology 2021Quote: ... Fractions containing each DNA pol of a high purity were pooled and concentrated using a 10K protein concentrator (Millipore). Concentrated proteins were preserved at −20 °C in the presence of 50% glycerol ...
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bioRxiv - Microbiology 2020Quote: ... the eluted proteins were loaded to 12% SDS-PAGE gel and then transferred to PVDF membranes (Millipore, Boston, USA). After blocking with 5% non-fat milk for 1 hour ...
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bioRxiv - Microbiology 2020Quote: ... 15 mL protein extracts were incubated in a 50 μL slurry of anti-FLAG M2 affinity gel (Sigma, A2220) and rotated at 4°C overnight ...
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bioRxiv - Molecular Biology 2020Quote: ... coli total protein extract were spotted within a pre-marked grid onto a 0.2 μm pore–size nitrocellulose membrane (Merk Millipore). The membrane was left to dry to fix the proteins for 30 min at room temperature ...
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bioRxiv - Microbiology 2020Quote: ... The epithelial cell proteins that remained bound to the fungal cells were eluted twice with 6 M urea (Sigma). The proteins were then separated on 10% SDS-PAGE and transferred to immun-Blot PVDF Membrane (BIO-RAD) ...
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bioRxiv - Molecular Biology 2021Quote: RNA immunoprecipitation assay was carried out according to the Magna RIP RNA-Binding Protein Immunoprecipitation Kit manufacturer’s instruction (Millipore). Briefly ...
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bioRxiv - Microbiology 2020Quote: ... Cells were incubated with 381.7 ng / ml mouse α-HAdV hexon protein antibody (Mab8052, Sigma-Aldrich, St. Louis, USA) and subsequently stained using 2 μg / ml goat α-mouse-AlexaFluor594 (A21203 or A32742 ...
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bioRxiv - Cancer Biology 2020Quote: Protein co-localization was assessed via proximity ligation assay (PLA) using the Duolink In Situ Red Starter Kit (Sigma) and PLA-approved primary antibodies for SC-35 (Abcam ...
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bioRxiv - Cell Biology 2021Quote: ... protein gel pieces were digested overnight with trypsin (Pierce) following reduction and alkylation with DTT and iodoacetamide (Sigma–Aldrich). The samples then underwent solid-phase extraction cleanup with a Oasis HLB μElution plate (Waters ...
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bioRxiv - Cell Biology 2021Quote: ... the alpacas were immunized subcutaneously and intradermally with 3 mL of 0.5 mg CTLA-4 protein and an equal volume of complete Freund’s adjuvant (0.5 mg; Sigma–Aldrich, USA). On days 91 and 112 ...
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bioRxiv - Immunology 2021Quote: ... the eluted proteins were loaded onto a 12% SDS-PAGE gel and then transferred to a PVDF membrane (Millipore). After blocking with 5% nonfat milk for 1 h ...
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bioRxiv - Biophysics 2021Quote: ... The fractions containing the capsid protein were pooled and concentrated using an Amicon Ultra-15 centrifugal filter (Merck Millipore), and the buffer was exchanged to a final storage buffer composed of 50 mM NaH2PO4/Na2HPO4 ...
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bioRxiv - Biophysics 2021Quote: ... The final purified proteins were concentrated using 50 kDa (MtCNNMΔC) or 100 kDa (MtCNNM) cutoff concentrators (Amicon Ultra, Millipore), flash frozen in liquid nitrogen ...
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bioRxiv - Biophysics 2021Quote: ... Peak fractions corresponding to soluble protein were pooled and concentrated to 4.5–5.5 mg/mL (Amicon Ultra 100-kDa cut off, Millipore Sigma). Protein purity was estimated as >95% by SDS-PAGE and protein concentration was measured spectrophotometrically (Implen Nanophotometer N60 ...
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bioRxiv - Biophysics 2021Quote: ... Further purification was achieved through capture of the cleaved protein by Strep-Tactin resin (IBA) and elution with 2.5mM d-desthiobiotin (Sigma). A final purification step was performed by size-exclusion chromatography using a HiLoad 16/60 Superdex 75 column (GE Healthcare ...
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bioRxiv - Biophysics 2020Quote: The protein was thawed and concentrate at 10 mg/ml in Amicon Ultra-15,10000Mr cut-off centrifugal concentrator (Millipore). 10 mM shikonin was added to the protein in a ratio of 5:1 and bond at 4°C for 2h ...
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bioRxiv - Biophysics 2022Quote: ... Protein was concentrated up to 40–60 mg/ml using a 100 kDa molecular weight cut-off concentrator (Millipore). The protein purity was checked by SDS-PAGE ...
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bioRxiv - Microbiology 2022Quote: ... The protein was purified affinity chromatography using Ni-NTA column (Yeasen, Wuhan, China) and concentrated using centrifugal ultrafiltration (Millipore). The concentration of recombinant protein was determined with bovine serum albumin (BSA ...
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bioRxiv - Biophysics 2022Quote: ... Non-specific protein adsorption to the coverslip was prevented by adding 0.005% (v/v) Tween-20 (P2287, Sigma-Aldrich) to the solution ...
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bioRxiv - Immunology 2022Quote: ... up to 5 × 107 cells were stained with monoclonal antibodies (Table 1) to cell surface proteins diluted in PBS containing 1 % BSA (Bovogen) and 0.1 % NaN3 (Sigma) (staining buffer ...
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bioRxiv - Microbiology 2022Quote: RNA immunoprecipitation (RIP) assays were performed using Magna RIP™ RNA-Binding Protein Immunoprecipitation Kit (EMD Millipore 17-700). HEK293T cells were plated on 15-cm dishes coated with poly-L-ornithine at a density of 4E4 cells/cm2 ...
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bioRxiv - Biochemistry 2022Quote: ... assay and immediately diluted (1:1) to a final protein concentration of 0.5 mg/mL with either 18O heavy (Sigma) or 16O light (Fisher ...
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Dissociation of β2m from MHC Class I Triggers Formation of Noncovalent, Transient Heavy Chain DimersbioRxiv - Cell Biology 2022Quote: ... were used at 14,000 x g and 4 °C to exchange purified protein samples to 150 mM ammonium acetate (99.99 %; Sigma-Aldrich), pH 7.2 ...
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bioRxiv - Molecular Biology 2022Quote: ... Argonaute proteins/small RNA complex were extracted from 50,000 synchronized worms and immunoprecipitated with anti-flag antibody (Sigma Aldrich) or GFP nanobody (Thermo) ...
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bioRxiv - Microbiology 2022Quote: ... of 3.5 mg/mL mixtures of purified STFK or STFK1628x proteins in complex with excess Fab fragments of nAbs were incubated in 0.01% (v/v) Digitonin (Sigma) and then loaded onto glow-discharged (60 s at 20 mA ...
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bioRxiv - Neuroscience 2022Quote: ... Proteins were separated by SDS-PAGE with 8% polyacrylamide gel and transferred to PVDF membrane (Millipore, Billerica, MA, USA). Membranes were blocked with 5% skim milk in Tris-buffered saline (20 mM Tris [pH8.0] ...
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bioRxiv - Immunology 2022Quote: Antibodies against the following proteins were obtained from commercial sources and used for immunoblots: anti-flag-tag (F3165, Sigma), anti-V5-tag (R960-25 ...
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bioRxiv - Cancer Biology 2022Quote: ... The cell types were probed with anti-Pan cytokeratin (Cat # 4545, Cell signalling technology, U.S.A) and anti-Fibroblast specific protein (Cat # F4771, Sigma, U.S.A) as primary antibodies ...
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bioRxiv - Biochemistry 2022Quote: ... Fractions of monomeric protein were collected and concentrated to around 30-50 µM using Amicon Ultra-0.5 ml Centrifugal Filters (Millipore), then separated into single-use aliquots ...
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bioRxiv - Bioengineering 2022Quote: ... The prenylated protein was subsequently purified by buffer exchange (PBS) with an Amicon Ultra-0.5 centrifugal filter (10 kDa cutoff, Millipore) for cell surface modification or the following conjugation reactions.