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Citations for Millipore Sigma :
9201 - 9250 of 10000+ citations for Recombinant Human ROR1 Protein His Avi tagged Biotinylated since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2020Quote: ... The proteins were separated on a 10% SDS-polyacrylamide gel and transferred onto an Immobilon PVDF membrane (Millipore). Membranes were blocked in 5% BSA/TBST at 4°C overnight ...
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bioRxiv - Biochemistry 2020Quote: ... 30μg of protein was used for SDS-PAGE and Western Blotting post transferring onto nitrocellulose membrane (Millipore; HATF00010). The blots were decorated with antibodies against GroEL (Enzo ...
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bioRxiv - Developmental Biology 2019Quote: Total protein was extracted from placenta using RIPA buffer with cOmplete™ protease inhibitor (Sigma, Oakville, ON, Canada) and 100mM sodium orthovanadate ...
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Sapogenin based self-assembly structures activating a non-apoptotic cell death via multiple pathwaysbioRxiv - Pharmacology and Toxicology 2021Quote: ... proteins were separated by SDS-PAGE electrophoresis and transferred to PVDF membranes (EMD Millipore, Thermo Fisher Scientific, US).
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bioRxiv - Neuroscience 2019Quote: ... Protein lysate was isolated in RIPA buffer supplemented with a protease and phosphatase-3 inhibitor cocktail (Sigma-Aldrich). Lysate (20 μg ...
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bioRxiv - Neuroscience 2019Quote: ... The pre-cleared supernatants were incubated with protein A- or G-Sepharose 4B beads (Sigma Aldrich or Amersham) and with primary antibodies for 2–4 h at 4 °C ...
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bioRxiv - Biochemistry 2021Quote: ... The eluted fractions containing purified protein samples were concentrated using a 10 kDa Amicon centrifugal filter (Merck Millipore) and the elution buffer suspending the purified protein was replaced with EDTA (10 mM)-PBS (1x ...
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bioRxiv - Molecular Biology 2020Quote: ... Proteins were transferred onto a Protran nitrocellulose membrane and probed with antibody clone 12D10 against puromycin (Millipore MABE343). Subsequently ...
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bioRxiv - Plant Biology 2021Quote: ... 5 µl of the denatured protein sample was manually spotted onto an activated PVDF Immobilon-P membrane (Millipore) mounted on top of a paper stack (bottom up ...
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bioRxiv - Molecular Biology 2019Quote: ... Band sizing was performed using the ImageJ 1.5 software (http://rsb.info.nih.gov/ij) and relative protein expression was determined by normalizing to GAPDH (1:10,000, MAB374, Millipore, Bedford, MA), for cell samples ...
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bioRxiv - Genetics 2019Quote: ... Proteins were reduced with 5 mM Tris(2-carboxyethyl)phosphine hydrochloride (Sigma-Aldrich, St. Louis, MO, product C4706) and alkylated with 55 mM iodoacetamide (Sigma-Aldrich ...
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bioRxiv - Cell Biology 2019Quote: Proteins in SDS-PAGE gels or native gels were transferred onto 0.45µm Immobilon-P PVDF membranes (Millipore. #IPVH00010). The membranes were blocked with 5% non-fat milk in TBST [20mM Tris (pH7.5) ...
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bioRxiv - Microbiology 2019Quote: ... Proteins were detected with anti-γH2A (1:1000) and mouse anti-Ef1α clone CBP-KK1 (1:25,000; Millipore) primary antibodies ...
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bioRxiv - Evolutionary Biology 2019Quote: ... The protein composition of the eluent was verified by western blot using Strep-tag II monoclonal antibodies (Novagen). The fractions containing MGE protein were pooled and concentrated to 1 mL by using a centrifugal concentrator (Sartorius ...
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bioRxiv - Evolutionary Biology 2019Quote: ... The protein composition of the eluent was verified by western blot using Strep-tag II monoclonal antibodies (Novagen). The fractions containing MGE protein were pooled and concentrated to 1 mL by using a centrifugal concentrator (Sartorius ...
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bioRxiv - Biochemistry 2020Quote: ... was determined in thermal shift experiments (30,31) For determination fluorescent protein stain SYPRO® Orange (5000x, Sigma-Aldrich) and CFX Connect Real-Time PCR Detection system (Bio-Rad ...
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bioRxiv - Genomics 2021Quote: ... Primary antibodies against the following proteins were used: SOX9 (1:50, AF3045, R&D Systems, and AB5535, Millipore), ERG (1:50 ...
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bioRxiv - Genetics 2021Quote: Three-fold diluted nuclear extracts were pre-cleared with 50 μL Protein-G agarose beads (Millipore, 16-266) for 1 hour at 4°C and then incubated overnight on a rotor with 3 μL anti-FLAG antibody (Sigma ...
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bioRxiv - Cell Biology 2021Quote: ... Proteins were separated by 12-20 % SDS–PAGE and transferred to a polyvinylidene fluoride membrane (Millipore, Billerica, MA). The membrane was blocked with TBS-T (20 mM Tris-HCl ...
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bioRxiv - Physiology 2021Quote: ... Proteins were resolved by SDS-PAGE on 8% acrylamide gels and electrotransferred onto Immobilon P (Millipore, Bedford, MA). Then ...
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bioRxiv - Microbiology 2020Quote: ... The purified protein was centrifuged by a 20 kDa-cut-off membranes made by Millipore (Milford, MA, USA) until the volume was approximately 1 mL ...
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bioRxiv - Biochemistry 2020Quote: ... The TEV-digested protein solution was concentrated with an Amicon Ultra 50K MWCO centrifugal filter unit (Millipore UFC901024) to 2 mL ...
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bioRxiv - Biochemistry 2021Quote: ... Protein elution from the beads was enforced by addition of 100 mM triethylammonium bicarbonate (pH = 8.5, Sigma Aldrich). To disaggregate the beads ...
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bioRxiv - Biochemistry 2021Quote: ... Controls lacking the protein included the flowthrough of ultrafiltration to establish the background or reduced glutathione (G4251, Sigma) as positive control of MBB modification ...
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bioRxiv - Biochemistry 2020Quote: ... Expression of CENP-C-GFP fusion proteins was induced by addition of 50 ng/ml doxycycline (Sigma Aldrich) for at least 24 hours.
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bioRxiv - Biochemistry 2021Quote: ... and the immunoprecipitated proteins were subjected to SDS-PAGE and immunoblotting with anti-Flag antibody (Cat# F1804, Sigma), anti-HA antibody (Cat # H6908 ...
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bioRxiv - Cancer Biology 2020Quote: ... An equal amount of proteins was loaded into SDS-PAGE gels and transferred to PVDF membranes (Millipore, MA). Membranes were blocked for 2 hours with 5% non-fat milk in PBST (0.1% Tween-20 in PBS) ...
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bioRxiv - Microbiology 2021Quote: ... and SARS2-71 [40] anti-S protein antibodies and HRP-conjugated goat anti-mouse IgG (Sigma Cat # A8924) in PBS supplemented with 0.1% saponin and 0.1% bovine serum albumin ...
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bioRxiv - Molecular Biology 2021Quote: ... The extraction of nuclear proteins has been performed using the CelLytic™ NuCLEAR™ Extraction kit (Sigma-Aldrich) complemented with PIC ...
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bioRxiv - Synthetic Biology 2021Quote: ... The enzyme and the expressed proteins were inactivated by adding 15 mM EDTA (Catalog No. E9884, Sigma-Aldrich) at 75°C for 10 minutes (T100 Thermal Cycler ...
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bioRxiv - Biochemistry 2020Quote: ... equal amounts of protein per sample were subjected to SDS-PAGE and transferred to a PVDF membrane (Millipore). For immunoprecipitations ...
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bioRxiv - Cancer Biology 2020Quote: The puromycin selection gene was replaced with green fluorescent protein (eGFP) cDNA in Lentiviral pLKO.1 plasmid (Sigma). shRNA oligos (Sigma ...
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bioRxiv - Cell Biology 2020Quote: Antibodies against the following proteins were used at indicated dilutions: anti-acTub (Sigma-Aldrich T7451, mouse 1:2,000), anti-Arl13b (Proteintech 17711-1-AP ...
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bioRxiv - Neuroscience 2020Quote: ... All proteins were resolved in 10 % to 12 % SDS-PAGE and transferred to Immobilon transfer membrane (EMD Millipore). Membranes were blocked in ChemiBlocker (EMD Millipore ...
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bioRxiv - Neuroscience 2020Quote: ... Equal amounts of protein samples were diluted at a ratio of 1:1 in Laemmli sample buffer (Sigma) and separated on pre-cast gel (4–12% Tris–glycineBio-Rad) ...
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bioRxiv - Neuroscience 2021Quote: ... Astrocytes were evaluated by immunostaining for glial fibrillary acidic protein (GFAP; monoclonal mouse anti-GFAP; 1:500; Millipore, Burlington ...
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bioRxiv - Biochemistry 2021Quote: ... Protein complexes were released from chromatin by digestion with 2 U/μl of Benzonase nuclease (E1014-25KU, Sigma) and sonicated for 5 min using a Diagenode sonicator with settings ...
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bioRxiv - Pathology 2021Quote: ... was homogenized with protein lysis buffer (50mM Tris-HCL pH 8.0, 150mM NaCl, 1% NP-40, 0.5mM PMSF(Sigma) and cOmplete protease inhibitor (Roche) ...
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bioRxiv - Biochemistry 2021Quote: ... About 20 μg protein was loaded in 12% SDS-PAGE and transferred to PVDF (0.22 μM) membrane (Millipore). Membranes were incubated with primary antibodies including anti-β-actin (1:10,000 dilution ...
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bioRxiv - Biochemistry 2021Quote: ... Expression of NK1R-miniGs/q70fusion protein was induced with addition of 4 μg/mL doxycycline hyclate (Sigma Aldrich) and enhanced with 20 mM sodium butyrate (Sigma Aldrich) ...
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bioRxiv - Cancer Biology 2021Quote: ... Protein was extracted from 5⨯106 cells with Pierce RIPA buffer (Thermo, 89901) containing proteasome inhibitor (Sigma, P8340) and quantified using the Pierce BCA Protein Assay Kit (ThermoFisher) ...
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bioRxiv - Cancer Biology 2020Quote: ... For protein detection: cell lysates were prepared in RIPA lysis buffer containing protease and phosphates inhibitors (Sigma-Aldrich) and Western blotting was performed as described (69) ...
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bioRxiv - Microbiology 2021Quote: ... The localisation of the codon-optimised NS5 protein was detected with a rabbit-anti-FLAG antibody (Sigma, Germany).
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bioRxiv - Immunology 2020Quote: ... were conjugated to the carrier protein Bovine Serum Albumin (BSA; Applichem or Sigma (low-endotoxin, fatty-acid free)) according to the protocol of Listenberger et al88 ...
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bioRxiv - Microbiology 2019Quote: ... The purified protein was concentrated to 15 mg ml−1 using a centrifugal concentration device (Amicon ultra, Millipore) before storage at 193K ...
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bioRxiv - Microbiology 2020Quote: ... Cells were stained with rabbit anti-protein VI 26 or mouse anti-E1A clone M73 (Millipore, 05-599) diluted in blocking buffer (10% goat serum in PBS ...
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bioRxiv - Microbiology 2021Quote: ... 1% Triton 100 and 1 × protein inhibitor) and subsequently precipitated with anti-Flag M2 Magnetic beads (Sigma, M8823) or GFP-Trap (Chromotek ...
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bioRxiv - Immunology 2020Quote: Protein lysates were separated on SDS-PAGE gels and transferred onto polyvinylidene fluoride (PVDF) membrane (Millipore, Etobicoke, ON). Membranes were blocked and probed ...
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bioRxiv - Immunology 2020Quote: ... Elastase limited proteolysis experiments were performed in a volume of 300 µL phosphate-citrate buffer pH 7.6 with 90 µg F1-V protein and 0.9 µg of Porcine Elastase (Milipore-Sigma). The reaction mixture was incubated at 37 °C for 1 hour with 20 µL aliquots taken every 5 minutes and dispensed into microcentrifuge tubes containing loading buffer ...
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bioRxiv - Immunology 2021Quote: ... 400 μg protein from the cytosolic lysate was incubated with 5 μg anti-FLAG mouse IgG1 (M2, Sigma) or mouse IgG1 control overnight at 4 °C ...