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Citations for Millipore Sigma :
9601 - 9650 of 10000+ citations for Recombinant Human ROR1 Protein His Avi tagged Biotinylated since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... Cleaved TREK1 or TRAAK protein was concentrated in 50 kDa molecular weight cutoff (MWCO) Amicon Ultra Centrifugal Filters (Millipore) and applied to a Superdex200 10/300 gel filtration column (GE Healthcare ...
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bioRxiv - Plant Biology 2020Quote: ... The expression of the target proteins was verified by western blot using ANTI-FLAG M2 Monoclonal Antibody (Sigma-Aldrich). ChIP assay was performed with ANTI-FLAG M2 Magnetic Beads (Sigma-Aldrich ...
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bioRxiv - Molecular Biology 2021Quote: ... coli genes for proteins bS6 and bL9 were PCR-amplified and cloned individually into the pET28(a) plasmid (Novagen). Recombinant single-cysteine proteins were then expressed ...
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bioRxiv - Immunology 2020Quote: ... one 10-cm dish of packaging cell line at roughly 70% density was transfected with the target construct and two supplementing vectors providing the VSV gag/pol and VSV-G-env proteins (6 μg of DNA each) using polyethylenimine (22.5 μg/ml, Sigma) and Polybrene (4 μg/ml ...
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bioRxiv - Molecular Biology 2021Quote: ... Fractions containing the proteins of interest were pooled and concentrated using Amicon Ultra-15 30K spin concentrators (Merck Millipore) before further purification using a HiLoad 16/600 Superdex 200 prep grade size exclusion column (GE Healthcare ...
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bioRxiv - Microbiology 2021Quote: ... Cells were centrifuged and resuspended (50 µl per 1 mL of sample) with SDS sample loading buffer (msGFP2 fusions) or BugBuster Protein Extraction Reagent with 0.1% lysonase (Millipore) followed by SDS loading buffer (3xFLAG fusions) ...
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bioRxiv - Pathology 2021Quote: ... Japan) were immunized (four times at 2-week intervals) with the purified protein conjugated to TiterMax Gold (Sigma-Aldrich). Rabbits were sacrificed under excess anesthesia with pentobarbital sodium (64.8 mg/kg) ...
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bioRxiv - Biophysics 2021Quote: ... A set of sequence ladders for the MS2 coat protein was obtained by addition of the four ddNTPs (Sigma) in a final concentration of 1 mM each to the M-MulV reaction mix containing dNTPs at 0.25 mM each ...
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bioRxiv - Pathology 2021Quote: ... 10 mM Na4P2O7) in the presence of protease inhibitors (completeTM, Mini, EDTA-free Protein inhibitor cocktail tablets, Roche, Sigma). Gut tissue and GDE were homogenized in the lysis buffer and sonicated three time for 30 seconds each at 10 μm amplitude ...
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bioRxiv - Molecular Biology 2020Quote: ... The proteins were detected with antibodies after western blotting: ABCE1-TAP with peroxidase anti-peroxidase (PAP) complex (Sigma-Aldrich) at 1:2,000 ...
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bioRxiv - Biochemistry 2020Quote: ... Fractions contained labelled protein were pooled and concentrated with Amicon Ultra-15 50 kDa cutoff centrifugal filter units (Millipore), frozen with liquid nitrogen and stored at −80°C ...
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bioRxiv - Physiology 2020Quote: ... the protein content of each sample was quantified using the Bradford assay (B6916, Sigma Aldrich, Saint Louis, MI, USA) with protein standards made from bovine serum albumin (ALB001.25 ...
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bioRxiv - Neuroscience 2020Quote: ... 5μg of total cell lysate proteins were resolved on 4-12% SDS-PAGE and transferred onto 0.45 μm polyvinyl difluoride membrane (Millipore) as described above under western blotting of EVs.
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bioRxiv - Immunology 2021Quote: ... the protein aliquots were transferred to the corresponding buffer with the use of Amicon Ultra-0.5 Centrifugal Filter Units (Millipore). The NaOCl sensitivities of intact cpYFP ...
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bioRxiv - Biochemistry 2020Quote: ... MBP-DIX proteins were eluted with three column volumes of lysis buffer containing 10 mM D-(+)-maltose monohydrate (Sigma) applied to loose resin under gentle rotation for 30 min ...
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bioRxiv - Cell Biology 2019Quote: ... After centrifuged at 20000g for 15mins and filtered through a low-protein binding 0.45µm PVDF membrane syringe filter (Millipore, #SLHVX13NL), the extracts were incubated with 30µl mouse anti-HA agarose beads (clone HA-7 ...
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bioRxiv - Biophysics 2019Quote: ... Fractions containing the protein were pooled and concentrated by centrifugal concentrators with 3 kDa molecular weight cutoff (EMD Millipore). The sample was polished by gel filtration (120 ml Superdex200 ...
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bioRxiv - Genetics 2019Quote: ... the column was inverted and bound proteins were eluted with 10 ml of buffer PT containing 0.1 mg/ml Flag peptide (Sigma). The eluate fractions were dialyzed against buffer A [25 mM Hepes-NaOH (pH 7.5) ...
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bioRxiv - Cancer Biology 2020Quote: ... Histone H3 lysine 27 trimethylated protein was detected using an anti-H3K27me3 antibody (rabbit polyclonal, Millipore, Burlington, MA, USA) diluted 1:500.
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bioRxiv - Genetics 2021Quote: ... Reversal of TetR fusion protein binding was achieved by addition of 1 μg/ml Doxycycline (final concentration / Sigma-D9891) to mESC culture medium.
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bioRxiv - Cell Biology 2021Quote: ... Proteins were digested with endoproteinase Lys-C (Wako, Osaka, Japan) and sequencing grade modified trypsin (modified sequencing grade, Sigma) followed by quenching with 1% trifluoroacetic acid (TFA) ...
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bioRxiv - Cell Biology 2020Quote: HMLE variants were washed with PBS prior to protein extraction with lysis buffer (PBS, protease inhibitor cocktail (Sigma-Aldrich), phosphatase inhibitor cocktail (Sigma-Aldrich) ...
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bioRxiv - Physiology 2021Quote: ... Total protein concentration was determined using the Bradford assay with bicinchoninic acid (BCA) reagent (Millipore Sigma, St. Louis, MO). Total protein (40 μg ...
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bioRxiv - Microbiology 2021Quote: ... Extracellular proteins (10 μg/mL; totally 700 mL) were filtered through a 0.22-μm nitrocellulose membrane (47-mm diameter; Millipore) and concentrated using the Amicon Ultra Centrifugal Filters to 7 mL (cut-off of 30 kDa) ...
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bioRxiv - Microbiology 2021Quote: ... The bacterial pellet was suspended in 5 mL lysis buffer (BugBuster Protein Extraction Reagent, Merck Millipore, UK; containing DNAse) and incubated at room temperature for 30 mins ...
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bioRxiv - Biochemistry 2020Quote: ... The bacterial pellet was suspended in 5 mL lysis buffer (BugBuster Protein Extraction Reagent, Merck Millipore, UK; containing DNAse) and incubated at room temperature for 30 mins ...
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bioRxiv - Molecular Biology 2021Quote: The lysates of HEK-293T cells transfected with circPTPN12 plasmid were incubated with Protein-A/G agarose beads (Millipore) and antibody against Ago2 (Abcam ...
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bioRxiv - Molecular Biology 2021Quote: RNA immunoprecipitation (RIP) experiments were performed using the Magna RIPTM RNA-Binding Protein Immunoprecipitation Kit (17-700, Millipore, USA) according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2021Quote: Protein was extracted from cells grown on 6-well culture plates using lysis buffer (CelLytic™ M, Sigma-Aldrich) supplemented with Halt™ protease/phosphatase inhibitor cocktail (Thermo Fisher Scientific) ...
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Structure-guided glyco-engineering of ACE2 for improved potency as soluble SARS-CoV-2 decoy receptorbioRxiv - Biochemistry 2021Quote: ... all proteins were centrifuged (16 000 g, 10 min, 20°C) and filtered (0.1 μm Ultrafree-MC filter, Merck Millipore). Proper performance of the MALS detectors was validated with bovine serum albumin ...
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bioRxiv - Biochemistry 2021Quote: ... SEC purified protein-DNA preparations were concentrated 10-fold by ultrafiltration (Amicon Ultra-15 30K centrifugal filter device, Millipore) and subjected to crystallization.
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bioRxiv - Biochemistry 2021Quote: ... Elution fractions containing the protein of interest were concentrated using a centrifugal filter (Amicon Ultra, MWCO 3 kDa, Sigma) before loading onto a size-exclusion Sepharose 200 column (Cytiva ...
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Prelimbic proBDNF facilitates memory destabilization by regulation of neuronal function in juvenilesbioRxiv - Neuroscience 2022Quote: ... prelimbic cortex were dissected and homogenized in lysis buffer (pH 7.4) containing a cocktail of protein phosphatase and proteinase inhibitors (Sigma). The samples were centrifuged at 14,000 r.p.m ...
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bioRxiv - Biochemistry 2020Quote: ... The bacterial pellet was suspended in 5 mL lysis buffer (BugBuster Protein Extraction Reagent, Merck Millipore, UK; containing DNAse) and incubated at room temperature for 30 mins ...
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bioRxiv - Cell Biology 2020Quote: ... Lysates were cleared by centrifugation at 16,000 g for 10 min and protein concentration was normalized by Amidoblack staining (Sigma). Western blotting was performed following standard protocols as described below ...
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bioRxiv - Microbiology 2021Quote: ... proteins were separated using a 10% SDS-PAGE gel and transferred onto a PVDF Immobilon-P membrane (Merck Millipore). The membrane was blocked with 5% w/v milk in TBST (50 mM Tris-HCl pH 7.6 ...
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bioRxiv - Microbiology 2021Quote: ... the alpaca was boosted twice with 250 μg S protein in 250 μl Freund’s incomplete adjuvant (F5506-10ML, Sigma). One week following the 2nd immunization ...
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bioRxiv - Immunology 2020Quote: For immunoblot analysis, the M-PER Protein Extraction Reagent (Pierce, USA) supplemented with a protease inhibitor cocktail (Sigma, USA) was used to lyse the cells ...
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bioRxiv - Molecular Biology 2021Quote: Protein depletion was induced by treating cells with a final concentration of 500 μM IAA (I5148-10G, Sigma-Aldrich). Time series experiments were performed by inducing protein degradation at different time points and collecting all samples at the end of the time course.
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bioRxiv - Immunology 2021Quote: ... 1 mM EDTA) buffer and OAS1 proteins were immunoprecipitated from the lysate using 20 µg anti-FLAG antibody (Sigma) and Dynabeads Protein G ...
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bioRxiv - Immunology 2020Quote: ... Fractions containing the trimeric HPIV3 F proteins were pooled and concentrated by centrifugation in an Amicon ultrafiltration unit (Millipore) with a 50 kDa molecular weight cut-off ...
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bioRxiv - Immunology 2021Quote: ... and pSARS-CoV-2 expressing SARS-CoV-2 S protein (0.6ug) (GenBank; MN908947.3) (Brouwer et al., 2020).Transfection was performed in 293T/17 cells using genejuice (Novagen, USA) transfection kit according to manufacturer’s protocol ...
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bioRxiv - Immunology 2020Quote: ... and the fractions with the target protein were mixed and concentrated using Amicon Ultra-15 Centrifugal filters (Merck Millipore) with a 3 kDa membrane cutoff (Figure 10).
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bioRxiv - Genetics 2020Quote: ... Protein samples were subjected to SDS-PAGE and wet-transferred to a 0.2 μM Immobilon-PSQ PVDF membrane (Millipore) at 200 mA for 90 minutes ...
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bioRxiv - Genetics 2020Quote: ... Proteins were detected using either secondary anti-mouse or anti-rabbit IgG coupled to peroxidase (Millipore, 1:5000 dilution), western bright ECL substrate (Advansta ...
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bioRxiv - Genetics 2020Quote: ... The purified covalent protein-DNA complexes were concentrated to about 0.1– 0.2 mM using Ultracel®-10K Centrifugal Filters (Millipore) in a buffer containing 20 mM Tris-HCl (pH 8.0) ...
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bioRxiv - Neuroscience 2020Quote: ... The following beads were used in immunoprecipitations: protein A-Sepharose 4 B Fast Flow from Staphylococcus aureus (P9424, Sigma), Protein G Sepharose 4 Fast Flow (17-0618-01 ...
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bioRxiv - Molecular Biology 2020Quote: ... Dialyzed DENV2C protein was concentrated using Amicon Ultra-4 Centrifugal Filter Units (10 kDa molecular weight cut off, Millipore). The concentration of the purified protein sample was determined using nanodrop.
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bioRxiv - Physiology 2020Quote: ... Membranes were washed in TBS-T and proteins were visualized using a chemiluminescent substrate (Sigma Aldrich, Oakville, ON, Canada).
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bioRxiv - Biochemistry 2022Quote: ... 4 mM DTT and 5% D2O at a protein concentration 1 mM using a Shigemi NMR tube (Sigma Aldrich). Data was collected on an ultrahigh field Bruker 900 MHz NMR spectrometer equipped with a triple resonance cryogenic probe ...