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Citations for Millipore Sigma :
9151 - 9200 of 10000+ citations for Recombinant Human MSLN Protein Fc tagged since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Genetics 2022Quote: ... The protein concentration in the supernatant was measured by the spectrophotometric Bradford method (Sigma Chemical Co., St Louis). Ascospore proteins extraction ...
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bioRxiv - Cell Biology 2022Quote: Protein isolation was performed by lysis of the cells in RIPA buffer (Sigma-Aldrich, St. Louis, United States), supplemented with complete protease and phosphatase inhibtors (Roche ...
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bioRxiv - Microbiology 2022Quote: ... stained with REVERT total protein stain (Licor Biosciences) and blocked with 3% bovine serum albumin (BSA) (Sigma-Aldrich) in phosphate buffered saline (PBS ...
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bioRxiv - Microbiology 2022Quote: ... Proteins were transferred to nitrocellulose membranes and probed with 1:1,000 a-FLAG primary (Sigma-Aldrich, clone M2) and 1:5,000 a-mouse secondary (Thermo Fisher ...
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bioRxiv - Biophysics 2022Quote: ... the protein was dialyzed overnight at 4° C (Cat. # D9527-100FT, 14 kDa MWCO cellulose membrane, Sigma Aldrich) into CP storage buffer (10 mM Tris-HCl pH 7.5 ...
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bioRxiv - Neuroscience 2022Quote: ... The instrument was calibrated using a protein standard mixture: β-amylase (Sigma-Aldrich, 56, 112 and 224 kDa), and thyroglobulin (Sigma-Aldrich ...
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bioRxiv - Systems Biology 2019Quote: ... Animal caps were dissected at stage 11 and lysed in the presence of protein phosphatase inhibitor cocktails (Sigma). Western blots were stained with rabbit anti-Smad1 antibody (1:1000 ...
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bioRxiv - Immunology 2019Quote: ... Samples were pre-cleared with 35 µl Protein A Agarose/Salmon Sperm DNA beads (Millipore, Temecula, CA, USA) at 4ºC for 30 min with continuous rotation ...
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bioRxiv - Physiology 2019Quote: ... Equal concentrations of protein from each sample were incubated with 40 μL of M2 agarose beads (Sigma, #A2220) for 3 h at 4°C ...
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bioRxiv - Cell Biology 2020Quote: ... Peak fractions of target proteins were collected and concentrated using Amicon Ultra-15 10K Centrifugal filters (Sigma-Aldrich).
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bioRxiv - Developmental Biology 2019Quote: ... All samples were then diluted with TE (1:1) and RNA and proteins were digested (RNAse, Sigma-Aldrich® ...
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bioRxiv - Microbiology 2019Quote: ... The solubilised protein (10 μl) was mixed with 290 μl of Quick Bradford reagent (Sigma Aldrich, NSW, Australia) and incubated at room temperature for 5 min 42 ...
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bioRxiv - Microbiology 2019Quote: ... The eluted proteins were visualized by Western Blotting with the following antibodies: anti-FLAG M2 from Sigma Aldrich, anti-GFP from Torrey Pines ...
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bioRxiv - Plant Biology 2020Quote: ... and proteins were detected by western blotting using either Anti-FLAG M2 monoclonal antibody-peroxidase conjugate (Sigma, A8592) at a dilution of 1:10000 ...
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Gatekeeper helix activates Golgi SM protein Sly1 and directly mediates close-range vesicle tetheringbioRxiv - Cell Biology 2020Quote: ... and resin-bound lipids were eluted from the beads with 50 µL of BugBuster protein extraction reagent (Millipore). The beads were again sedimented ...
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bioRxiv - Biochemistry 2020Quote: ... The protein eluent was concentrated to 500 µL using an Amicon centrifugal concentrator (50 kD cut-off, Millipore) and loaded onto a Superdex 200 10/300 GL column (GE Healthcare ...
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bioRxiv - Biochemistry 2020Quote: ... Fractions containing pure protein were pooled and concentrated in a 3,500 MWCO Amicon-Ultra centrifugal filter (Millipore Sigma) at 4°C ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... 6 mg RBD protein in 3 mL PBS were mixed with equal-volume Freund’s incomplete adjuvant (Sigma-Aldrich) for the first boosting ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... 3 mg RBD protein in 3 mL PBS were mixed with equal-volume Freund’s complete adjuvant (Sigma-Aldrich) for priming ...
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The Arabidopsis F-box protein FBW2 degrades AGO1 to avoid spurious loading of illegitimate small RNAbioRxiv - Cell Biology 2021Quote: ... Identical amounts of crude extracts were incubated with prebound @AGO1 (5μg) PureProteome Protein A magnetic beads (30μL; Millipore) for 2 hours at 7 rpm in the cold room ...
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bioRxiv - Molecular Biology 2020Quote: ... Bound protein was eluted three times for 30 min by incubation with 0.3 mg/ml 3xFLAG peptide (Sigma) in BC100 at 4°C with end-over-end rotation ...
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bioRxiv - Molecular Biology 2021Quote: ... The protein was eluted using Buffer A with 150 mM NaCl and 200 μg/ml 3XFLAG peptide (Sigma). Peak fractions were pooled ...
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bioRxiv - Plant Biology 2021Quote: Equal amounts of total proteins were separated on 12% SDS-PAGE and transferred to PVDF membranes (Millipore, #IPVH00010). The concentration of proteins was determined by Bradford assay ...
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bioRxiv - Genomics 2020Quote: ... 20 µl protein extracts diluted 1:1 (v/v) with ice-cold 2x Laemmli sample buffer (Sigma-Aldrich) were separated by 12% SDS-PAGE ...
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bioRxiv - Biochemistry 2021Quote: For the measurement of the total protein content 205 μL of Bradford Reagent (Sigma-Aldrich, cat. n. B6916) was pipetted to the 50 μL of BSA standard/thawed cell lysates ...
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bioRxiv - Cancer Biology 2021Quote: ... Proteins were resolved by SDS-PAGE and electroblotted by wet transfer onto Immobilion-P membranes (Millipore Sigma, MA). Membranes were blocked in 5% milk in TBS-T (50 mM Tris pH 7.6 ...
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bioRxiv - Microbiology 2020Quote: ... Proteins were transferred to PVDF membrane and probed with M2 anti-FLAG antibody (Sigma; 1 in 1,000 dilution) and HRP-conjugated goat anti-mouse antibody (1 in 250,000 dilution) ...
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bioRxiv - Molecular Biology 2020Quote: ... Proteins were separated by sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) and transferred to nitrocellulose membranes (Millipore). Membranes were blocked for 1 hour in PBST (1× PBS ...
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bioRxiv - Biochemistry 2020Quote: ... The proteins were separated on a 10% SDS-polyacrylamide gel and transferred onto an Immobilon PVDF membrane (Millipore). Membranes were blocked in 5% BSA/TBST at 4°C overnight ...
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bioRxiv - Microbiology 2021Quote: ... Bound proteins were detected by western blot using anti-His antibody and streptavidin-HRP conjugate (SIGMA, reference GERPN1231).
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bioRxiv - Biochemistry 2020Quote: ... 30μg of protein was used for SDS-PAGE and Western Blotting post transferring onto nitrocellulose membrane (Millipore; HATF00010). The blots were decorated with antibodies against GroEL (Enzo ...
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bioRxiv - Developmental Biology 2019Quote: Total protein was extracted from placenta using RIPA buffer with cOmplete™ protease inhibitor (Sigma, Oakville, ON, Canada) and 100mM sodium orthovanadate ...
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Sapogenin based self-assembly structures activating a non-apoptotic cell death via multiple pathwaysbioRxiv - Pharmacology and Toxicology 2021Quote: ... proteins were separated by SDS-PAGE electrophoresis and transferred to PVDF membranes (EMD Millipore, Thermo Fisher Scientific, US).
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bioRxiv - Neuroscience 2019Quote: ... Protein lysate was isolated in RIPA buffer supplemented with a protease and phosphatase-3 inhibitor cocktail (Sigma-Aldrich). Lysate (20 μg ...
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bioRxiv - Neuroscience 2019Quote: ... The pre-cleared supernatants were incubated with protein A- or G-Sepharose 4B beads (Sigma Aldrich or Amersham) and with primary antibodies for 2–4 h at 4 °C ...
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bioRxiv - Biochemistry 2021Quote: ... The eluted fractions containing purified protein samples were concentrated using a 10 kDa Amicon centrifugal filter (Merck Millipore) and the elution buffer suspending the purified protein was replaced with EDTA (10 mM)-PBS (1x ...
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bioRxiv - Molecular Biology 2020Quote: ... Proteins were transferred onto a Protran nitrocellulose membrane and probed with antibody clone 12D10 against puromycin (Millipore MABE343). Subsequently ...
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bioRxiv - Plant Biology 2021Quote: ... 5 µl of the denatured protein sample was manually spotted onto an activated PVDF Immobilon-P membrane (Millipore) mounted on top of a paper stack (bottom up ...
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bioRxiv - Molecular Biology 2019Quote: ... Band sizing was performed using the ImageJ 1.5 software (http://rsb.info.nih.gov/ij) and relative protein expression was determined by normalizing to GAPDH (1:10,000, MAB374, Millipore, Bedford, MA), for cell samples ...
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bioRxiv - Genetics 2019Quote: ... Proteins were reduced with 5 mM Tris(2-carboxyethyl)phosphine hydrochloride (Sigma-Aldrich, St. Louis, MO, product C4706) and alkylated with 55 mM iodoacetamide (Sigma-Aldrich ...
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bioRxiv - Cell Biology 2019Quote: Proteins in SDS-PAGE gels or native gels were transferred onto 0.45µm Immobilon-P PVDF membranes (Millipore. #IPVH00010). The membranes were blocked with 5% non-fat milk in TBST [20mM Tris (pH7.5) ...
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bioRxiv - Microbiology 2019Quote: ... Proteins were detected with anti-γH2A (1:1000) and mouse anti-Ef1α clone CBP-KK1 (1:25,000; Millipore) primary antibodies ...
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bioRxiv - Evolutionary Biology 2019Quote: ... The protein composition of the eluent was verified by western blot using Strep-tag II monoclonal antibodies (Novagen). The fractions containing MGE protein were pooled and concentrated to 1 mL by using a centrifugal concentrator (Sartorius ...
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bioRxiv - Evolutionary Biology 2019Quote: ... The protein composition of the eluent was verified by western blot using Strep-tag II monoclonal antibodies (Novagen). The fractions containing MGE protein were pooled and concentrated to 1 mL by using a centrifugal concentrator (Sartorius ...
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bioRxiv - Biochemistry 2020Quote: ... was determined in thermal shift experiments (30,31) For determination fluorescent protein stain SYPRO® Orange (5000x, Sigma-Aldrich) and CFX Connect Real-Time PCR Detection system (Bio-Rad ...
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bioRxiv - Genomics 2021Quote: ... Primary antibodies against the following proteins were used: SOX9 (1:50, AF3045, R&D Systems, and AB5535, Millipore), ERG (1:50 ...
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bioRxiv - Genetics 2021Quote: Three-fold diluted nuclear extracts were pre-cleared with 50 μL Protein-G agarose beads (Millipore, 16-266) for 1 hour at 4°C and then incubated overnight on a rotor with 3 μL anti-FLAG antibody (Sigma ...
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bioRxiv - Cell Biology 2021Quote: ... Proteins were separated by 12-20 % SDS–PAGE and transferred to a polyvinylidene fluoride membrane (Millipore, Billerica, MA). The membrane was blocked with TBS-T (20 mM Tris-HCl ...
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bioRxiv - Physiology 2021Quote: ... Proteins were resolved by SDS-PAGE on 8% acrylamide gels and electrotransferred onto Immobilon P (Millipore, Bedford, MA). Then ...
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bioRxiv - Microbiology 2020Quote: ... The purified protein was centrifuged by a 20 kDa-cut-off membranes made by Millipore (Milford, MA, USA) until the volume was approximately 1 mL ...