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Citations for Millipore Sigma :
9251 - 9300 of 10000+ citations for Recombinant Human MSLN Protein Fc tagged since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2022Quote: ... The presence of over-expressed protein was confirmed using monoclonal anti-poly histidine alkaline phosphatase antibody (Sigma-Aldrich) at 1:20,000 dilution.
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bioRxiv - Microbiology 2022Quote: ... and the data normalized to total protein content that was determined by the bicinchoninic acid (BCA) assay (Sigma).
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bioRxiv - Plant Biology 2022Quote: ... GST-OsIDD12 and GST-OsIDD13 fusion proteins were expressed in Escherichia coli BL21(DE3) Rosetta strain (Novagen, USA). Frozen cells were extracted with 100 μl of extraction buffer containing 50 mM Tris-HCl (pH 7.6) ...
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bioRxiv - Neuroscience 2022Quote: ... and then purified from this culture media using Protein G Sepharose beads (Cat# GE17-0618-01, Millipore Sigma), as described previously (Naito et al. ...
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bioRxiv - Molecular Biology 2023Quote: ... 5% BSA) and pre-cleared with 30 μL of Protein A agarose beads (Millipore Cat No. 16-125) for 1 h at 4°C ...
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bioRxiv - Neuroscience 2023Quote: ... hERG channel protein was stably expressed in HEK-293 cells purchased from Millipore (Cat. No. CYL3006; Billerica, MA). Assay methods have been previously described (32–34) ...
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bioRxiv - Neuroscience 2022Quote: ... Twenty µg of protein samples were loaded and separated on 4–12% Criterion Bis-Tris Gels (Millipore, USA) electrophoresis and transferred to polyvinylidene fluoride (PVDF ...
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bioRxiv - Molecular Biology 2022Quote: ... The protein was then eluted in the same buffer that was supplemented with 20 mM maltose (Sigma-Aldrich). The fractions were pooled and concentrated on a Millipore concentrator (WMCO 50 kDa) ...
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bioRxiv - Molecular Biology 2022Quote: ... The eluted proteins were then analysed by Coomassie staining using Brilliant Blue G colloidal concentrate (Sigma Aldrich, B2025) on 4–12% NuPAGE Bis-Tris gels (Life Technologies).
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bioRxiv - Molecular Biology 2022Quote: ... Protein was concentrated to ∼500 μL using Amicon Ultra-15 Centrifugal Filter Units (Merck Millipore MWCO 3 kDa). The concentration of proteins was determined using JASCO V730 UV-vis spectrophotometer at 280 nm ...
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bioRxiv - Cell Biology 2022Quote: ... Proteins were separated by SDS-PAGE and analyzed by WB using anti-Flag (Sigma-Aldrich F3165; 1:7500), anti-RPL3 (Proteintech 11005-1-AP ...
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bioRxiv - Plant Biology 2022Quote: ... Either total or immunoprecipitated proteins were separated on a SDS-PAGE gel and transferred to PDVF membrane (Millipore) for immunoblot analysis using anti-HA (ab18181 ...
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bioRxiv - Systems Biology 2022Quote: The proteins were extracted from the frozen cell pellets by lysing the cells with 1% SDC (Sigma, D6750) in HNN Buffe pH 7.8 (50 mM HEPES ...
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bioRxiv - Physiology 2022Quote: ... by adding 20 μL of magnetic beads (Magna ChIP Protein G Magnetic Beads C #16-662, Sigma-Aldrich) and a combination of two anti BMAL1 antibodies ...
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bioRxiv - Plant Biology 2022Quote: ... the same total protein extracts were incubated with Endoglycosidase H at 37 °C for 3 h (Sigma-Aldrich) according to the manufacturer’s instructions ...
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bioRxiv - Plant Biology 2022Quote: ... Fractions containing the proteins were concentrated with Amicon® Ultra-15 Centrifugal Filters Ultracel®-100K (Merck-Millipore, Burlington ...
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bioRxiv - Biophysics 2022Quote: ... The protein solution was filtered through 0.22 μm hydrophilic polyethersulfone (PES) membrane filter (Cat#SLGP033NS, Merck Millipore, USA) and stored as 1.0 mL aliquots at −80 °C until crystallization experiments ...
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bioRxiv - Cell Biology 2022Quote: ... An equal 20-40 ug of proteins were separated by SDS PAGE and transferred to PVDF membranes (Millipore). Western blots were performed as standard procedures ...
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bioRxiv - Neuroscience 2024Quote: ... Proteins were separated via 4-15% SDS-PAGE electrophoresis and transferred to a PVDF membrane (IPVH 00010, Millipore). Membranes were immersed in blocking solution (5% nonfat milk in 0.1 % Tween 20 in 1X TBS ...
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bioRxiv - Cell Biology 2024Quote: ... Target proteins were probed using their respective antibodies (Table S1) in 5% Bovine Serum Albumin (BSA, Sigma-Aldrich) overnight at 4°C ...
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bioRxiv - Molecular Biology 2024Quote: ... Proteins were extracted from the residue debris pellets and quantified using a BCA kit (Sigma-Aldrich, Taufkirchen, Germany). Lipid profiling was performed using a Sciex ExionLC AD coupled to a Sciex ZenoTOF 7600 under control of Sciex OS 3.0 (Sciex ...
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bioRxiv - Cell Biology 2024Quote: ... the proteins were expressed in the BL21 strain from Escherichia coli at 37°C in LB medium (Sigma) supplemented with 100 µg/mL ampicillin until OD600 between 0.4 and 0.5 ...
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bioRxiv - Physiology 2024Quote: ... The protein samples were separated by 10% SDS-PAGE and then transferred to polyvinylidene fluoride membranes (Millipore, USA). The membranes were blocked with 10% non-fat dry milk in Tris-buffered saline with Tween-20 for 1 h at room temperature and then incubated with primary antibodies overnight at 4℃ (anti-phospho-PKA ...
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bioRxiv - Microbiology 2024Quote: ... the proteins on the gels were transferred onto an activated polyvinylidene difluoride (PVDF) membrane (Immobilon from Millipore, # IPFL00005) and then blocked with 1X TBS-0.1% Tween-20-5% dry milk for 1 hour at room temperature on a rocker ...
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bioRxiv - Cancer Biology 2024Quote: ... Proteins were then transferred to a 0.45 μm Immobilon-FL PVDF membranes (IPFL00010; EMD Millipore, Burlington, MA, USA) using the eBlot™ L1 Fast Wet Transfer System (GenScript ...
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bioRxiv - Molecular Biology 2024Quote: ... 1X PPI] and precleared by 30 μL protein A/G agarose beads (Merck-Millipore, 16-157/16-201) for 1h ...
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bioRxiv - Molecular Biology 2023Quote: ... The RIG-I protein fractions were pooled and concentrated using a 30 kDa Amicon Ultra filter (Millipore #UFC903008). Lastly ...
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bioRxiv - Molecular Biology 2024Quote: ... or with 1-5 μg of the appropriate antibody and then with Protein G Sepharose beads (Sigma, P3296). The following day ...
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bioRxiv - Molecular Biology 2024Quote: ... Ribonucleoprotein (RNP) complexes (dCas9-gRNA) were formed by mixing the annealed gRNA constructs with dCas9 protein (Sigma-Aldrich) in 2:1 molar ratio (600 nM ...
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bioRxiv - Biochemistry 2024Quote: ... Each reaction mixture constituted protein at the desired concentration with 1.25 mM Tris (2,2’-bipyridyl)dichlororuthenium(II)hexahydrate (Sigma, CAS No ...
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bioRxiv - Neuroscience 2024Quote: 10 µg of protein was loaded per sample and membrane was probed with antibodies against APOE (Sigma; AB947), RHEB (Abcam ...
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bioRxiv - Biochemistry 2024Quote: ... appropriate AGO:RNA bands were excised from the gel and protein digested with Proteinase K (RPROTK-RO, Sigma Aldrich) and released RNA isolated via phenol:chloroform phase separation ...
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“Identification of microRNAs regulated by E2F transcription factors in human pluripotent stem cells”bioRxiv - Developmental Biology 2024Quote: ... Equal amounts of protein were electrophoresed on a 12% SDS– polyacrylamide gel and transferred to PVDF membranes (Millipore). Blots were blocked for 1 hour at RT (room temperature ...
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bioRxiv - Biochemistry 2023Quote: ... was incubated for 2 hrs at 4 °C with Protein A/G Mix Magnetic Beads (Cat# LSKMAGAG10, Millipore). After washing with dilution buffer ...
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bioRxiv - Cell Biology 2024Quote: Protein samples were separated by 10-12% SDS-PAGE and electrotransferred to nitrocellulose membranes (Merck Millipore, Cat # ISEQ00010). Immunoblotting was carried out as follows ...
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bioRxiv - Immunology 2023Quote: ... The eluted protein was concentrated to 500 μl with a 100-kDa molecular mass cut-off concentrator (Millipore). Concentrated apo-KSHV-GPCR-Gi complex was loaded onto a Superdex 200 Increase 10/300 GL column (GE Healthcare ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... Protein quantification for each sample was conducted by BCA Assay in accordance with kit instructions (Millipore Sigma 71285M).
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bioRxiv - Biochemistry 2024Quote: ... Protein was concentrated to 10 mL using an Amicon Ultra-15 10K MWCO Centrifugal Filter Unit (Merck Millipore) and loaded into SnakeSkin Dialysis Tubing ...
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bioRxiv - Biophysics 2024Quote: ... The instrument was calibrated using a protein standard mixture: β-amylase (Sigma-Aldrich, 56, 112 and 224 kDa), and thyroglobulin (Sigma-Aldrich ...
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bioRxiv - Biochemistry 2024Quote: ... Proteins were separated by SDS-polyacrylamide gel electrophoresis (PAGE) electroblotted onto a polyvinylidene difluoride (PVDF) membrane (Millipore, IPFL00010). PVDF membrane was masked with Intercept Blocking Buffer (LI-COR ...
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bioRxiv - Developmental Biology 2023Quote: ... The proteins bound to the beads were reduced with 10 mM Tris(2-carboxyethyl)phosphine hydrochloride (Sigma, C4706) at 40°C for 30 min and alkylated with 20 mM Iodoacetamide (Sigma ...
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bioRxiv - Cell Biology 2023Quote: ... The fractions corresponding to the protein complexes were concentrated using a 500-μl 30K MWCO concentrator (Millipore-Sigma).
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bioRxiv - Cell Biology 2023Quote: ... The fractions corresponding to the protein complexes were concentrated using a 500-μl 30K MWCO concentrator (Millipore-Sigma).
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bioRxiv - Cancer Biology 2022Quote: ... Ras protein level in the pull down was analyzed by by immunoblotting using RAS antibody (Sigma-Aldrich #OP40). Total was lysate was also subjected to immunoblotting to determine RAS expression level as the input.
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bioRxiv - Molecular Biology 2023Quote: ... An equivalent amount of protein lysate was subjected to SDS-PAGE and immunoblotting with antibodies against FLAG (Sigma), V5 (Cell Signaling ...
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bioRxiv - Microbiology 2023Quote: ... Protein was detected using HRP-conjugated anti-mouse secondary antibody (Amersham) and Luminata Forte Western HRP substrate (Millipore).
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bioRxiv - Microbiology 2023Quote: ... The remaining RNA was combined with 25 μl of Magna ChIP Protein A+G magnetic beads (Merck Millipore) coated in 5μl of anti-m6A antibody (Merck Millipore ...
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bioRxiv - Neuroscience 2023Quote: ... 30-100 μg of protein were resolved by SDS-PAGE and transferred to nitrocellulose membranes (Protran, Sigma-Aldrich). Membranes were blocked in PBS or TBS containing 3% BSA or 5% skimmed milk ...
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bioRxiv - Developmental Biology 2022Quote: ... Purified protein was dialysed against water then concentrated using an Amicon Ultra-Ultracel 5 kDa centrifugal filter (Millipore). Antibodies were raised in two guinea pigs by Eurogentec.
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bioRxiv - Systems Biology 2023Quote: ... Proteins were then separated on 12% SDS PAGE and transferred to a PVDF membrane (Millipore Sigma, 0.2 μM). Membranes blocked with 5% milk were then probed with anti-HA (1:2000 ...