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Citations for Millipore Sigma :
8951 - 9000 of 10000+ citations for Recombinant Human MSLN Protein Fc tagged since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2023Quote: ... Equivalent amounts of protein were resolved by SDS-PAGE and transferred to polyvinylidene fluoride microporous membrane (Millipore), blocked with 1.5% BSA in H20 containing 0.1% Tween-20 (TBS-T) ...
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bioRxiv - Cancer Biology 2023Quote: ... Protein samples were denatured with 4x Laemmli Sample buffer with 10% Beta-Mercaptoethanol (Sigma-Aldrich, cat: #M6250) followed by 10 min boiling at 95℃ ...
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bioRxiv - Cancer Biology 2023Quote: ... The protein was separated by 8%–12% SDS-PAGE gels and transferred onto polyvinylidene fluoride membranes (Millipore). Membranes were blocked in 5% BSA in TBS for 1 hour at room temperature and then incubated with primary antibodies overnight at 4C ...
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bioRxiv - Biophysics 2023Quote: ... The protein solution was concentrated using a 30 kDa cutoff centrifugal filter unit (Millipore, Burlington, MA, U.S.A.) and loaded into a Superdex200 10/300 GL gel filtration column (G.E ...
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bioRxiv - Cancer Biology 2023Quote: ... Fractions containing target protein were desalted and concentrated on Amicon Ultra 10K centrifugal filter units (Merck Millipore), and the final protein preparation was stored in PBS containing 0.05% Triton X-100 at 4°C.
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bioRxiv - Biochemistry 2023Quote: ... The protein expression was induced by addition of 0.1 mM isopropyl-β-D-thiogalactoside (IPTG, Sigma-Aldrich) for 6 hours at 37°C ...
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bioRxiv - Immunology 2023Quote: ... The gene encoding the Streptococcus pyogenes M4 protein (uniprot ID P13050) was cloned into pET28_a (+) vector (Novagen). The M4 endogenous N-terminal signal peptide and C-terminal cell wall sorting signal were not included in the construct and a C-terminal hexahistine-tag was added ...
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bioRxiv - Molecular Biology 2023Quote: ... Protein bands were visualized on X-ray film by electro-chemiluminescence (Immobilon Western HRP Substrate, WBKLS0500, Millipore). For the isolation of chromatin-enriched proteins ...
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bioRxiv - Synthetic Biology 2023Quote: ... The pellets were resuspended in 50 μL of BugBuster Protein Extraction Reagent (Millipore Sigma, SKU 70584-4) and vortexed on Benchmark Scientific Incu-Mixer MP at room temperature ...
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bioRxiv - Neuroscience 2023Quote: ... The protein sample was concentrated using 100kDa MWCO Amicon® Ultra-15 Centrifugal Filter Unit (EMD Millipore) to a final concentration of ∼0.8mg/ml and filtered by 0.22 µm pore size PVDF membrane prior to deposition on the grid for EM imaging.
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Aromatase in adipose tissue exerts an osteoprotective function in male mice via phosphate regulationbioRxiv - Physiology 2024Quote: ... The separated proteins were transferred by electrophoresis to Immobilon-P polyvinylidene difluoride membrane (Millipore, Billerica, MA, USA) and treated with diluted antibodies ...
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bioRxiv - Biophysics 2024Quote: NMR experiments were performed on protein in 90% NMR Buffer and 10% deuterated water (99.8%, Sigma-Aldrich). The PHL4effector domain concentration was 73.2 µM for the 13C and 15N labeled sample and 98.2 µM for the 15N labeled sample ...
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bioRxiv - Biophysics 2024Quote: ... The cleaved WW4-HECT protein was concentrated (Amicon Ultra-15 centrifugal filter, 10 kDa cutoff; EMD Millipore), filtered ...
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bioRxiv - Cancer Biology 2024Quote: ... Bound material was recovered after incubation with 15 μL of protein G beads (Millipore, cat # 16-266) at 4°C ...
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bioRxiv - Biochemistry 2024Quote: ... The DNA was digested by incubation with benzonase (Sigma, 25 U per expected 1 mg of protein) for 30 min at room temperature ...
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bioRxiv - Biochemistry 2024Quote: The NSP3(1-110) protein was then immobilized on Anti-Flag M2 Affinity gel (Sigma Aldrich, A2220), and the cell lysates from stable cell line 293T-N ...
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bioRxiv - Biochemistry 2024Quote: ... Bound protein was eluted off agarose gel with 30-50 μL 1X 3XFLAG peptide (Sigma catalog #F4799) at 4 °C with rotation for 1 h ...
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bioRxiv - Biophysics 2024Quote: ... Then 495 µL of the protein sample was mixed with 55 µL 99.8% deuterated water (Sigma-Aldrich). The diffusion NMR NMR samples for the PHL4FL and PHL4effector domain were prepared by concentrating pure non-isotopically labeled ...
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bioRxiv - Cell Biology 2024Quote: ... The eluted proteins were collected and concentrated to 500 µL with a 30K MWCO concentrator (EMD Millipore). Concentrated Ndel1 was then purified via SEC on a Superdex200 10/300 column (Cytiva ...
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bioRxiv - Biophysics 2024Quote: ... cells were resuspended and lysed in BugBuster® Master Mix Protein Extraction Reagent (Millipore Sigma, Cat# 71456) at room temperature following the manufacturers recommendation ...
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bioRxiv - Cell Biology 2024Quote: ... Twenty micrograms of total protein were separated on 10% SDS-PAGE and transferred onto PVDF membranes (Millipore). The resulting membranes were blocked for 1 hour at 37°C with TBST buffer (12 mM Tris-HCl ...
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bioRxiv - Microbiology 2024Quote: For top-down EfpL measurements the proteins were desalted on the ZipTip with C4 resin (Millipore, ZTC04S096) and eluted with 50 % (v/v ...
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bioRxiv - Cancer Biology 2024Quote: ... Equal amounts of proteins were separated by SDS-PAGE and transferred to PVDF membranes (Millipore, Cat. IPVH00010). Membranes were incubated with primary antibodies in 5% BSA in 1x TBST buffer overnight at 4°C ...
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bioRxiv - Microbiology 2024Quote: ... Proteins were then transferred to nitrocellulose membrane for immunodetection using mouse anti-puromycin (1:1000,12D10, MABE943 Sigma) and mouse anti-actin (1:25 ...
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bioRxiv - Biochemistry 2024Quote: ... Protein fractions from a single peak were collected and concentrated in 50 kDa MWCO concentrator (Millipore Sigma), flash frozen in liquid nitrogen and stored at −80 °C until further use ...
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bioRxiv - Biochemistry 2024Quote: ... Protein fractions from a single peak were collected and concentrated in 50 kDa MWCO concentrator (Millipore Sigma), flash-frozen in liquid nitrogen and stored at −80 °C until further use ...
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bioRxiv - Microbiology 2024Quote: ... 0.5 µM protein and 5 mg/ml of one of the following carbohydrates was used: maltose (Sigma), maltotriose (Carboexpert-BoGH13BSus/SusA ...
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bioRxiv - Cell Biology 2024Quote: ... proteins were separated using SDS-PAGE and subsequently transferred to a polyvinylidene fluoride membrane (PVDF, Millipore Corporation). Then blocked PVDF membrane with blocking buffer (5% fat-free milk ...
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bioRxiv - Molecular Biology 2024Quote: ... 0.5 mL of the cleared lysate (∼2.5 mg total protein) was combined with anti-Flag (Sigma, 1804) coated Dynabeads Protein G (Invitrogen 10003D ...
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bioRxiv - Molecular Biology 2024Quote: ... Equivalent amounts of sample proteins in Laemmli buffer (With reducing agent, 2-Mercaptoethanol (Sigma-Aldrich, CAT# 8057400005) were electrophoresed on 4-20% Mini-PROTEAN® TGX Stain-Free gels (Bio-rad ...
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bioRxiv - Molecular Biology 2024Quote: ... Chromatin concentrations were normalized among samples and pre-cleared with Protein G agarose beads (Millipore, 100 μL). The chromatin samples were split in two and diluted with IP buffer (10 mM Tris-HCl pH = 8.0 ...
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bioRxiv - Molecular Biology 2024Quote: ... 200 µg of protein was reduced with 5 mM Tris(2-carboxyethyl) phosphine hydrochloride (TCEP) (Sigma Aldrich) for 2 min and alkylated with 20 mM chloroacetamide (CAA ...
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bioRxiv - Molecular Biology 2024Quote: ... diluted and precleared with 30 μL protein A/G agarose beads (Merck-Millipore, 16-157/16-201) for 1h at 4°C ...
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bioRxiv - Plant Biology 2024Quote: ... Purified proteins were further concentrated using Amicon Ultra centrifugal filter device (3 kDa MW cut-off, Millipore). Protein quantity was measured using Bradford method (Sigma-Aldrich) ...
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bioRxiv - Cell Biology 2024Quote: ... The eluted protein solution was in turn transferred to an ultrafiltration column (Amicon Ultra-15, Merck Millipore) and concentrated by centrifugation ...
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bioRxiv - Molecular Biology 2024Quote: ... Proteins were separated by SDS-PAGE and transferred onto 0.45 µm pore size Immobilon PVDF membrane (Millipore). PVDF membranes were blocked with TBS-T (50 mM Tris ...
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bioRxiv - Synthetic Biology 2024Quote: ... Purified protein was concentrated using an Amicon Ultra-4 Centrifugal 50 kDa Filter Units (Sigma Aldrich NV). Protein was quantified against a standard curve made with Bovine Serum Albumin (BSA) ...
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bioRxiv - Developmental Biology 2024Quote: ... that had been pre-coated with Protein A from Staphylococcus aureus (Sigma P6031; 100 ng/μl PA) and contained 800 μl culture media (Leibovitz’s L15 Medium ...
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bioRxiv - Molecular Biology 2024Quote: ... and in N2B27 serum-free media containing Mitogen-Activated Protein Kinase (MEK) inhibitor PD0325901 (1 μM) (Millipore), Glycogen Synthase Kinase 3 (GSK3 ...
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bioRxiv - Genomics 2020Quote: ... RRID:AB_2798177) overnight at 4°C or 1:10,000 dilution of monoclonal mouse anti-human β-actin (clone AC-74, Sigma-Aldrich, Cat. No. A2228, RRID:AB_476997) for 1 hour at room temperature ...
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bioRxiv - Cell Biology 2021Quote: βII-spectrin-KO CRISPR cell lines were generated as follows: a predesigned vector containing a human βII-spectrin-targeting gRNA and eCas9-2A-tGFP was purchased from Millipore Sigma (MISSION gRNA ID: HSP00000399630). 24 hours after transfection of this plasmid ...
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bioRxiv - Bioengineering 2020Quote: ... MSCs embedded in the PEG-NB gels were stained for α-SMA with FITC-conjugated mouse monoclonal anti-human α-SMA (F3777, Sigma; dilution 1:300), cellular extra domain-A (ED-A ...
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bioRxiv - Cell Biology 2021Quote: Human peripheral blood mononuclear cells (PBMC) were isolated from whole blood by Ficoll density gradient using Ficoll Plaque Premium (Sigma GE Healthcare, #17-544-02) or BD Vacutainer CPT Tubes (BD ...
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bioRxiv - Neuroscience 2021Quote: ... hiPSC lines were dissociated to form embryoid bodies and cultured on mitomycin C-treated SNL murine fibroblast feeder cells in standard human embryonic stem cell culture (hESC) medium (DMEM/F12; Sigma Aldrich, St. Louis, MO, USA) containing 20% knockout serum replacement (Thermo Fisher Scientific ...
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bioRxiv - Neuroscience 2020Quote: ... codon optimized for expression in human cell lines and modified to include the human ER localization signal was cloned into the expression vector (Catalog Nr: OGS606-5U) under the EF1α promoter (Sigma Aldrich, St. Louis, MO, USA). Positive clones were verified by Sanger sequencing (Microsynth ...
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bioRxiv - Cancer Biology 2020Quote: ... On this plasmid a single primer PCR with primer 5’-GCA AGC CCT GAA AGC GCA AG-3’ and the cDNA human HIF-1α in a p3XFLAG-CMV™-10 expression vector (Sigma, St Louis, MO, USA) (Gort et al. ...
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bioRxiv - Immunology 2020Quote: ... plasma samples were incubated at a dilution of 1:100 and bound antibodies were detected with goat anti-human IgM/HRP (Sigma: cat. A6907, 1:6’000 dilution), goat anti-human IgG/HRP (Thermo Fisher ...
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bioRxiv - Microbiology 2022Quote: ... and grown as undifferentiated 3D cultures in 500 µL IntestiCult™ Organoid Growth Medium (OGM) Human (STEMCELL™ Technologies, cat# 06010) supplemented with 10 µM Y-27632 (Sigma-Aldrich, cat# Y0503). Undifferentiated monolayers were produced by plating single cell suspensions obtained from 7 days old highly dense 3D cultures ...
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bioRxiv - Pathology 2023Quote: ... was performed in a representative subset of our participants using an adapted version of the MILLIPLEX® Human High Sensitivity T Cell Magnetic Bead Panel (Millipore Sigma, Burlington, MA, USA). The MAGPIX® Luminex analyzer (Luminex Corporation ...
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bioRxiv - Cancer Biology 2023Quote: ... Primary human mesenchymal stem cells (MSCs) were cultured on gelatin coated flasks for 2-3 days in Alpha-MEM (Sigma-Aldrich, St. Louis, MO, USA) supplemented with 1% pen-strep (Sigma-Aldrich ...