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Citations for Millipore Sigma :
9001 - 9050 of 10000+ citations for Recombinant Human MSLN Protein Fc tagged since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Pathology 2023Quote: ... was performed in a representative subset of our participants using an adapted version of the MILLIPLEX® Human High Sensitivity T Cell Magnetic Bead Panel (Millipore Sigma, Burlington, MA, USA). The MAGPIX® Luminex analyzer (Luminex Corporation ...
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bioRxiv - Cancer Biology 2023Quote: ... Primary human mesenchymal stem cells (MSCs) were cultured on gelatin coated flasks for 2-3 days in Alpha-MEM (Sigma-Aldrich, St. Louis, MO, USA) supplemented with 1% pen-strep (Sigma-Aldrich ...
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bioRxiv - Immunology 2023Quote: ... After washing, IgE antibodies were detected using biotinylated goat anti-human IgE (Vector, Burlingame, CA, USA) and streptavidin peroxidase (Sigma-Aldrich, St. Louis, MO, USA). The wells were then washed again with PBST buffer and incubated with 100 ul 3,3’,5,5’- Tetramethylbenzidine (Kirkegaard and Perry Laboratories ...
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bioRxiv - Immunology 2024Quote: ... and IFN-γ were measured in the full Generation R cohort using the Human High Sensitivity T-Helper Cells Custom 5-plex assay from Millipore (Millipore, St. Charles, MO, USA) on the Luminex™ 100 system (Luminex ...
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bioRxiv - Microbiology 2024Quote: ... blood mononuclear cells were isolated by density gradient centrifugation from buffy coats of human healthy donors (Ficoll-Paque Premium, Sigma Aldrich, St. Louis, MI, USA). Monocytes were purified from peripheral blood mononuclear cells by positive selection with magnetic CD14 MicroBeads (Miltenyi Biotech ...
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bioRxiv - Plant Biology 2020Quote: ... 5 μg of purified His fusion protein (PuBZR1-His) was bound to Ni-NTA His binding resin (Novagen). GST fusion proteins containing PuACO1 (PuACO1-GST ...
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bioRxiv - Cancer Biology 2021Quote: Proteins were extracted from cells in either radioimmunoprecipitation assay (RIPA) buffer 1 mM protease inhibitor cocktail (Sigma-Aldrich). Proteins were loaded on PVDF membranes ...
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bioRxiv - Cancer Biology 2021Quote: Proteins were extracted from cells in either radioimmunoprecipitation assay (RIPA) buffer 1 mM protease inhibitor cocktail (Sigma-Aldrich). Samples were heated at 100 °C for 10 min in a 4X loading buffer ...
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bioRxiv - Microbiology 2020Quote: ... The digested proteins were visualized in a 10% acrylamide gel stained with Coomassie Brilliant Blue R (Sigma Aldrich)
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bioRxiv - Cell Biology 2020Quote: ... Proteins were visualized with 1:20,000 anti-rabbit IgG (whole molecule)-peroxidase secondary antibody (Sigma; A0545, lot 022M4811) and SuperSignal West Femto Maximum Sensitivity Substrate (Thermo Fisher ...
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bioRxiv - Cell Biology 2020Quote: ... Proteins were transferred from gels to PVDF membrane (Immobilon-FL, pore size 0.45 μm, Millipore, catalogue number IPFL00010) for immunoblotting ...
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bioRxiv - Cell Biology 2020Quote: ... a fraction of Proteins was separated on 12% SDS-PAGE gels and transferred to Immobilon PVDF membranes (Millipore). The membranes were blocked in 5% milk or 5% BSA (depending on the primary antibody) ...
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bioRxiv - Cell Biology 2020Quote: ... The lysates were cleared from insoluble chromatin and were subjected to immunoprecipitation with protein A agarose beads (Millipore) for 1.5 hrs at 4°C ...
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bioRxiv - Immunology 2021Quote: ... The protein was concentrated using a 30 kDa MWCO Amicon ultra-15 centrifugal filter (Sigma, cat. no. UFC903024), and stored at −70°C ...
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bioRxiv - Immunology 2021Quote: ... The eluted protein was incubated with 0.5 mM ADP and 0.5 mM MCC950 (Millipore Sigma, Cat. no: 538120), 0.5 mM dATP or no additives for 30 min on ice ...
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bioRxiv - Developmental Biology 2021Quote: ... 20 ul protein A/G beads pre-saturated with 5 mg/ml BSA in PBS (Sigma, A-3311) were washed in RIPA buffer and subsequently added to the chromatin/antibody mix at 4°C ...
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bioRxiv - Biophysics 2022Quote: ... and subsequently blocked with 0.5 % non-fat dried milk (AppliChem) in TBST using the Snap i.d.® 2.0 Protein Detection System (Millipore). The blot was then incubated for 10 min at room temperature with a 1:1000 dilution of rabbit polyclonal anti-ASIC1a antibody (OSR00097W ...
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bioRxiv - Neuroscience 2021Quote: ... protein extracts from SCN cultures were examined by SDS-PAGE immunoblotted with anti-UBE3A monoclonal antibody (E8655, Sigma), or anti-FLAG monoclonal antibody (F1804 ...
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bioRxiv - Molecular Biology 2020Quote: ... Immunoprecipitation of FLAG-AGO2 was performed with Dynabeads Protein G (Life) and anti-FLAG M2 mouse (Sigma, F3165) for 3h at 4ºC with rotation ...
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bioRxiv - Molecular Biology 2021Quote: ... Protein concentrations for the whole cell lysates and the unbound fractions were determined using Bradford reagent (Sigma-Aldrich). For immunoprecipitation ...
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bioRxiv - Molecular Biology 2021Quote: ... anti-GAPDH (1:2000, Protein Tech, #10494-1-AP) and anti-rabbit IgG-AP (1:20000, Sigma, #A3687)
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bioRxiv - Cell Biology 2022Quote: ... Bound proteins were eluted with 5 packed bead volumes of 150 ng/μL 3xFLAG peptide (Sigma-Aldrich, F4799) in TBS for 30 min at 4 °C with rotation.
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Rab35 Governs Apicobasal Polarity Through Regulation of Actin Dynamics During Sprouting AngiogenesisbioRxiv - Developmental Biology 2022Quote: ... Cells were then incubated for 15 minutes in 2% BSA with globular actin-binding protein GC globulin (Sigma). Following incubation ...
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bioRxiv - Immunology 2022Quote: ... Single colonies were inoculated and protein expression was induced using isopropyl-β-D-thiogalacto-pyranoside (IPTG, Sigma Aldrich). Cell lysates were screened for protein expression using SDS-PAGE followed by Coomassie blue staining ...
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bioRxiv - Microbiology 2020Quote: ... ii) 300 μg C g−1 13C-labelled Algal crude protein extract-98 atom% 13C (ISOTEC, Sigma-Aldrich), iii ...
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bioRxiv - Immunology 2021Quote: ... Proteins were electrophoretically separated and transferred to polyvinylidene fluoride (PVDF) membranes (Immobilon P, Merck Millipore, Burlington, MA, USA). Blocking treatment was performed using the PVDF Blocking Reagent for Can Get Signal (Toyobo ...
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bioRxiv - Molecular Biology 2021Quote: ... Protein levels of wild-type or mutant Rad6 were determined by immunoblotting using anti-Flag M2 antibody (Sigma).
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bioRxiv - Physiology 2019Quote: ... The silver stained protein spots were de-stained using the ProteoSilver Plus Stain Kit (Sigma-Aldrich, MO, USA), and in-gel enzymatic digestion was done overnight at 37°C using Trypsin Singles ...
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bioRxiv - Physiology 2019Quote: ... Gels containing protein spots were then stained using the ProteoSilver Plus Silver Stain Kit (Sigma-Aldrich, MO, USA) according to manufacturer instructions ...
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bioRxiv - Molecular Biology 2019Quote: ... and 0.1% NP-40 substitute (Fluka)) and added to 5-7 µL of protein A sepharose beads (Sigma) in a 50% slurry from the vendor that had been washed in IPP150 and conjugated to ∼5-10 µg of affinity-purified or protein A-purified anti-Prp43p ...
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bioRxiv - Neuroscience 2019Quote: ... Proteins were separated using SDS-PAGE and transferred to a polyvinylidene difluoride (PVDF) membrane (Immobilon-FL, EMD Millipore). Western blotting was performed using rabbit-anti-SRGAP2 N-terminal (raised against residues 193-205 ...
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bioRxiv - Molecular Biology 2020Quote: ... Purified protein was buffer exchanged into TBS using a 10,000 Da molecular weight cut-off filter (Millipore-Sigma) through 3 successive washes ...
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bioRxiv - Molecular Biology 2020Quote: ... Purified protein was buffer exchanged into TBS using a 10,000 Da molecular weight cut-off filter (Millipore-Sigma) through 3 successive washes ...
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bioRxiv - Molecular Biology 2020Quote: ... Incubated RdRp complex protein was concentrated with a 100 kDa molecular weight cut-off Centrifugal Filter (Millipore Corporation) and then size-separated by a Superdex S200 10/300 GL column in 25 mM HEPES pH 7.4 ...
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bioRxiv - Molecular Biology 2019Quote: ... DNA-cross-linked proteins were immunoprecipitated (1% kept as input) using 5 μg of NF-kB/p65 (Millipore) or control mouse IgG antibody ...
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bioRxiv - Cell Biology 2019Quote: ... Protein extracts were then loaded on 10% SDS-page acrylamide gels and transferred on PVDF membrane (Millipore #IPVH00010) overnight ...
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bioRxiv - Cell Biology 2020Quote: ... the proteins were eluted by adding 125 ng/ml FLAG peptide to the OG-containing buffer (Sigma-Aldrich) and incubating the beads for 30 min per round of elution ...
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bioRxiv - Developmental Biology 2020Quote: ... Proteins were then trypsinised by incubating with 5 µg/ml trypsin in 25 mM triethylammonium bicarbonate (Sigma-Aldrich) overnight ...
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bioRxiv - Developmental Biology 2019Quote: ... then resuspended in 2ml papain solution [0.75% v/v of papain (25mg/ml, 17U/mg protein, Sigma-Aldrich), 40μg/ml DNase I type IV ...
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bioRxiv - Microbiology 2019Quote: ... The eluted protein complex was concentrated using a 100 kD MWCO Amicon Ultra Centrifugal Filter Unit (EMD Millipore). The concentrated protein complex was further purified using size-exclusion chromatography with a Superose-6 Increase 10/300-GL (GE Life Sciences ...
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bioRxiv - Microbiology 2019Quote: ... The eluted LytH protein was concentrated using a 50 kD MWCO Amicon Ultra Centrifugal Filter Unit (EMD Millipore). The LytH protein was further purified using size-exclusion chromatography with a Superdex 200 Increase 10/300 GL column (GE Life Sciences ...
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bioRxiv - Neuroscience 2019Quote: ... 700-1000 µg total protein was incubated overnight at 4°C with 1:100 anti-FLAG (Sigma, F1804) or anti-Myc (Sigma ...
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bioRxiv - Cancer Biology 2020Quote: ... Protein samples were denatured and then separated on SDS-PAGE and transferred to PVDF membrane (Millipore, Burlington, USA). After blocking by non-fat milk for 1 hour ...
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bioRxiv - Microbiology 2020Quote: ... Fractions containing the protein were pooled and concentrated using an Amicon with a 3 KDa cut-off (Millipore). The protein was conserved in 10 mM Tris-HCl pH7.63 and 33% glycerol ...
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bioRxiv - Biochemistry 2020Quote: ... the proteins were concentrated using Amicon Ultra centrifugal filter devices (30 kDa molecular weight cut-off, EMD Millipore), and were quantified by Pierce™ BCA Protein Assay Kit (Thermo Scientific) ...
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bioRxiv - Microbiology 2019Quote: ... at a concentration of 1 μg/ml or anti-ubiquitylated protein antibodies (clone FK2, Millipore Sigma #04-263) at a dilution of 1:400 for 3 hours at room temperature.
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A key interfacial residue identified with in-cell structure characterization of a class A GPCR dimerbioRxiv - Biochemistry 2019Quote: Protein samples were separated on a 10% SDS-PAGE and transferred to nitrocellulose membranes (Millipore Corporation, Billerica, USA). The non-denaturing condition was achieved with the use of SDS-free sample buffer and without sample boiling before loading sample ...
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bioRxiv - Biochemistry 2020Quote: ... Lamin proteins were detected using mouse anti-myc antibodies (clone 4A6, Millipore cat #05-724; 1:10000 dilution) decorated with goat anti-mouse secondary IRDye 680RD antibodies (LI-COR) ...
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bioRxiv - Biochemistry 2020Quote: ... The supernatant (total protein concentration ∼3 mg/ml) was incubated with ANTI-FLAG M2 agarose beads (Sigma Aldrich) at 4°C for 1 h with agitation ...
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bioRxiv - Genetics 2021Quote: ... Complexes were diluted to 2.5uM in a protein storage buffer composed of 50% (v/v) glycerol (Sigma G5516), 100 mM NaCl (Fisher Scientific S271-3) ...