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Citations for Millipore Sigma :
9301 - 9350 of 10000+ citations for Recombinant Human MSLN Protein Fc tagged since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2023Quote: ... at 4°C overnight and then with protein A agarose/salmon sperm DNA slurry (Millipore, Cat# 16-157) at 4°C for 1h ...
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bioRxiv - Plant Biology 2023Quote: ... The eluted protein was concentrated to 1 mg/ml using Amicon Ultra 10 kDa MWCO filters (Millipore Sigma). Size-exclusion chromatography was performed using a Superose 6 10/300 GL column (Cytiva ...
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bioRxiv - Cell Biology 2023Quote: ... equal amounts of protein were subjected to SDS polyacrylamide gel electrophoresis and transferred to nitrocellulose membrane (Millipore Sigma) by semi-dry transfer ...
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bioRxiv - Developmental Biology 2023Quote: Proteins were separated on denaturing polyacramide gels before transfer to a 0.45 μm Immobilon-P PVDF membrane (Millipore) in transfer buffer (25 mM Tris ...
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bioRxiv - Molecular Biology 2023Quote: ... Proteins were extracted from 400,000 to 500,000 cells with RIPA buffer with the addition of proteinase inhibitor (Sigma) and phosphatase inhibitors (Sigma) ...
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bioRxiv - Evolutionary Biology 2023Quote: ... Sized proteins were concentrated to >50 mg mL-1 using 10 kDa molecular weight cut-off concentrators (Millipore), flash frozen on liquid nitrogen ...
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bioRxiv - Neuroscience 2023Quote: ... The primary antibodies used were anti-glial fibrillary acidic protein (GFAP) antibody (dilution; 1:100, Sigma-Aldrich (G6171), St ...
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bioRxiv - Genetics 2023Quote: ... Immunoprecipitation was performed on total-protein lysate using 1 μg of rabbit polyclonal TTC29 antibody (HPA061473, Sigma-Aldrich) and 15 μl of Protein A/G magnetics beads (Bio-Ademtec ...
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bioRxiv - Microbiology 2023Quote: ... the eluted proteins were concentrated in an Amicon®Ultra-15 Centrifugal Filter Unit (Millipore; 10 kDa cutoff) and incubated with the SUMO protease Ulp1 (Ulp1:His-SUMO-tH2 = 1:500 w/w ...
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bioRxiv - Microbiology 2023Quote: ... and cells were probed using primary antibodies to VZV proteins IE62 (mouse mAb; EMD Millipore MAB8616; 1:200), capsid-ORF23 (polyclonal rabbit ...
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bioRxiv - Cell Biology 2023Quote: ... Spastin protein was resolved on 8% SDS-PAGE gels transferred to Immobilon®-FL PVDF membranes (Millipore; 05317) using a wet blot transfer system (BioRad) ...
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bioRxiv - Developmental Biology 2023Quote: ... The following primary antibodies were used for protein blotting: mouse anti-c-Myc antibody (Sigma-Aldrich, 05-724MG), mouse anti-Flag antibody (Sigma-Aldrich ...
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bioRxiv - Systems Biology 2023Quote: Well plates containing single-cell protein lysates were digested with 2 ng of Trypsin (Sigma cat. nr. T6567) supplied in 1 μL of digestion buffer (100 mM TEAB pH 8.5) ...
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bioRxiv - Cell Biology 2023Quote: ... equal amounts of protein were subjected to SDS polyacrylamide gel electrophoresis and transferred to nitrocellulose membrane (Millipore Sigma) by semi-dry transfer ...
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bioRxiv - Molecular Biology 2023Quote: ... Proteins were probed using mouse anti-FLAG antibody (Monoclonal anti-FLAG M2 antibody, Sigma-Aldrich, F3165, 1:2000), or anti-beta actin (Abcam ...
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bioRxiv - Neuroscience 2023Quote: ... After the protein was collected the following day and concentrated using 10 kDa MWCO Amicon Ultra (Merck Millipore). The protein concentration was monitored periodically during the process using Bio-Rad Protein Assay Dye Reagent Concentrate (Bio-Rad ...
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bioRxiv - Neuroscience 2023Quote: ... Expression of the eGFP protein and the PAK1 mutant was induced by doxycycline (2 μg/mL, Sigma-Aldrich, St ...
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bioRxiv - Biochemistry 2023Quote: mAb TnI-1 (IgG1) was purified from mouse hybridoma ascites fluid using Protein G chromatography (Millipore Sigma P7700). TnI-1 IgG eluted with 50 mM glycine-HCl ...
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bioRxiv - Biochemistry 2022Quote: ... The expression of the proteins was assessed by western blot using antibodies raised against the T7 epitope (Novagen) and GAPDH (Abcam).
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bioRxiv - Biochemistry 2023Quote: ... The peak fractions containing the protein were pooled and concentrated in centrifugal concentrators (CLVG01230, Merck Millipore, Cork, Ireland) to a final concentration of 30 mg/ml for crystallization ...
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bioRxiv - Biophysics 2023Quote: ... Protein degradation was induced by treating cells with a final concentration of 400 µM dTAG-13 (Sigma SML2601) for 2 hrs or 6 hrs (2 hrs ...
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bioRxiv - Cell Biology 2022Quote: ... N-glycosylated proteins were enriched by incubating cell lysates containing protease inhibitor and 1,10-phenanthroline (Sigma-Aldrich, #131377) with 20µl concanavalin A Sepharose beads (Sigma-Aldrich ...
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bioRxiv - Cell Biology 2023Quote: ... HIV-Tat protein transduction motif (YGRKKRRQRRR) was subcloned into a pET-46 Ek/LIC vector (EMD Millipore #71335). The construct encoding a GST-tagged caltubin was subcloned into a pGEX-4T-1 vector (Amersham #28954549) ...
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bioRxiv - Developmental Biology 2023Quote: ... Proteins were separated on GenScript SurePAGETM Bis-Tris gels and blotted on the Immobilon-P transfer membrane (Millipore). The membrane was blocked with 5% skim milk and incubated with primary antibodies ...
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bioRxiv - Developmental Biology 2023Quote: ... Cells were then incubated for 15 minutes in 2% BSA with globular actin-binding protein GC globulin (Sigma). Following incubation ...
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bioRxiv - Bioengineering 2023Quote: ... Proteins were run on NuPAGE 4%–12% Bis-Tris Gel (Novex) and transferred onto PVDF membranes (Millipore Sigma) and probed with antibodies ...
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bioRxiv - Neuroscience 2023Quote: ... Homogenate of 50 μg was added to a process control of 800 ng of yeast enolase protein (Sigma) which was then reduced with 20 mM DTT and alkylated with 40 mM IAA ...
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bioRxiv - Microbiology 2023Quote: ... Proteins were filter-concentrated using centrifugation and a 10-kDa or 30-kDa cut-off column (Millipore Sigma) and dialyzed at 4 °C for ∼24 h against Dialysis Buffer (50 mM Tris-HCl pH 7.5 ...
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bioRxiv - Cell Biology 2023Quote: ... Samples were pre-cleared for 1 h with protein A Sepharose CL-4B (Sigma-Aldrich, 17-0780-01) beads blocked with 0.2 µg/µl sonicated herring sperm DNA (Thermo Fisher ...
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Characterization of an evolutionarily distinct bacterial ceramide kinase from Caulobacter crescentusbioRxiv - Biochemistry 2023Quote: ... The dialyzed protein was concentrated using an Amicon Ultra centrifugal filter (10 kDa molecular weight cutoff) (Millipore Sigma). The protein concentration was determined using the BCA Protein Assay Kit (Pierce).
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bioRxiv - Neuroscience 2023Quote: ... Supernatants were transferred into 100 kilodalton (kDa) molecular weight cut-off (MWCO) protein concentrators (Millipore-Sigma, UFC 805024) and concentrated from 5 ml to 0.5 ml ...
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bioRxiv - Cell Biology 2023Quote: ... A total of 1mg protein lysate was incubated with 50 µl suspension of monoclonal anti-HA agarose (Millipore). After overnight incubation ...
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bioRxiv - Immunology 2023Quote: ... The protein was further purified by anion exchange chromatography using a SOURCE™ 15 Q column (MILLIPORE SIGMA) and eluted in the final protein buffer containing 20mM Tris-HCl pH 7.5 ...
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bioRxiv - Microbiology 2023Quote: ... Purified proteins were concentrated to >15 mg ml−1 using 30-kDa MWCO centrifugal filter units (Millipore Sigma), aliquoted ...
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bioRxiv - Microbiology 2023Quote: ... Purified proteins were concentrated to >10 mg mL−1 using a 30 kDa MWCO centrifugal filter (Millipore Sigma), aliquoted ...
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bioRxiv - Genomics 2023Quote: ... (b) Reduce background signal by pre-clearing with 20µL of Protein A/G magnetic beads (Millipore 16-663) per 1mL of sample for 2 hours at 4°C with rotation ...
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bioRxiv - Biochemistry 2023Quote: ... The GCL reaction was stopped by precipitation of proteins with 200 mM 5-sulfosalicylic acid (Sigma Aldrich, 247006) and then put onto ice for 20 min ...
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bioRxiv - Neuroscience 2023Quote: ... Supernatants were transferred into 100 kilodalton (kDa) molecular weight cut-off (MWCO) protein concentrators (Millipore-Sigma, UFC 805024) and concentrated from 5 ml to 0.5 ml ...
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bioRxiv - Cell Biology 2023Quote: ... Nucleic acids from the protein samples were removed by incubating the samples with Benzonase (Millipore Sigma, Cat. No.E1014) for 20 min on ice before use in Sun1 interacting pulldown assay.
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bioRxiv - Microbiology 2023Quote: ... Enriched proteins were denatured in U/T buffer (6 M urea, 2 M thiourea, 1 mM DTT (Sigma), 10 mM HEPES ...
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bioRxiv - Molecular Biology 2023Quote: Protein isolation was performed by lysis of the cells in RIPA buffer (Sigma-Aldrich, St. Louis, United States), supplemented with complete protease and phosphatase inhibitors (Roche ...
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bioRxiv - Cancer Biology 2023Quote: ... The nuclear protein lysate then was pre-cleared by adding 2 μL of poly D[IC] (Sigma-Aldrich) 25 µg/µL and 20 μL of High Capacity Strepavidin agarose beads (Thermofisher #20357) ...
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bioRxiv - Evolutionary Biology 2023Quote: ... Total protein was quantified following the method described by Bradford using bovine serum albumin (BSA, Sigma cat. 7906) as standard ...
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bioRxiv - Biochemistry 2024Quote: ... Each reaction mixture constituted protein at the desired concentration with 1.25 mM Tris (2,2’-bipyridyl)dichlororuthenium(II)hexahydrate (Sigma, CAS No ...
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bioRxiv - Cell Biology 2024Quote: ... the proteins were expressed in the BL21 strain from Escherichia coli at 37°C in LB medium (Sigma) supplemented with 100 µg/mL ampicillin until OD600 between 0.4 and 0.5 ...
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bioRxiv - Neuroscience 2024Quote: 10 µg of protein was loaded per sample and membrane was probed with antibodies against APOE (Sigma; AB947), RHEB (Abcam ...
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bioRxiv - Physiology 2024Quote: ... The protein samples were separated by 10% SDS-PAGE and then transferred to polyvinylidene fluoride membranes (Millipore, USA). The membranes were blocked with 10% non-fat dry milk in Tris-buffered saline with Tween-20 for 1 h at room temperature and then incubated with primary antibodies overnight at 4℃ (anti-phospho-PKA ...
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bioRxiv - Neuroscience 2023Quote: Total protein from BFP expressing iNeurons was extracted using CelLytic™ M Cell Lysis Reagent (Sigma-Aldrich, #C2978) with PhosSTOP™ (Roche ...
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bioRxiv - Biophysics 2023Quote: Plasmids for analyte proteins were constructed with gBlocks (Integrated DNA Technologies) inserted into the pET-49b(+) plasmid (Novagen), with a dihydrofolate reductase domain ...
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bioRxiv - Biophysics 2023Quote: ... The purified protein (in 25 mM piperazine-N,N′-bis(2-ethanesulfonic acid) (PIPES, P-1851, Sigma-Aldrich) pH 6.8 ...