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Citations for Millipore Sigma :
9051 - 9100 of 10000+ citations for Recombinant Human MSLN Protein Fc tagged since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Genomics 2020Quote: ... Approximately 10 mg of total protein was added to 30 μl anti-FLAG M2 magnetic beads (Sigma # M8823) prepared according to the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2020Quote: ... anti-parvalbumin (PV;P3088) and anti-glial fibrillary acidic protein (GFAP;G3893) antibodies were obtained from Sigma-Aldrich; bromodeoxyuridine (BrdU ...
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bioRxiv - Molecular Biology 2021Quote: ... The elution of PAN-protein complexes was performed using 0.5 μl Benzonase nuclease (125U final, EDM Millipore 71206) in 1 ml of 0.35 M NaCl for 2 h at 37 °C (45 ...
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bioRxiv - Microbiology 2021Quote: ... MERS S-protein was detected using a monoclonal anti-FLAG M2-HRP conjugated antibody (SIGMA, A8592 @ 1:2000) which bound to a C-terminal FLAG-tag ...
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bioRxiv - Microbiology 2021Quote: ... Equal protein amount were used to perform SDS-PAGE and transferred onto polyvinylidene difluoride membranes (PVDF) (Millipore, USA) by the semi-dry transfer (Bio-Rad ...
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bioRxiv - Biochemistry 2021Quote: ... Proteins were extracted using RIPA buffer with protease inhibitors (cOmplete™, EDTA-free Protease Inhibitor Cocktail; Millipore Sigma). Sample input was normalized using a Bradford protein assay that was calibrated with BSA standards ...
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bioRxiv - Cell Biology 2021Quote: ... Expression of EGFP fusion proteins was then induced by addition of 1 µg/mL doxycycline (Sigma-Aldrich, D9891) and EGFP expressing cells were positively selected by FACS ...
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bioRxiv - Biochemistry 2021Quote: 100 bacterial homologs of nucleobase/cation symporter 2 (NCS2) proteins were cloned into a modified pET28 plasmid (Novagen) with either an N- or a C-terminal deca-histidine tag and a TEV protease recognition site at the central facility of the New York Consortium on Membrane Protein Structure (NYCOMPS ...
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bioRxiv - Biochemistry 2020Quote: ... the protein was treated by 0.5 mg/mL drICE protease and incubated with 2 mM NADPH (Sigma-Aldrich) for 2 h at 4 °C ...
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bioRxiv - Bioengineering 2021Quote: ... Fractionation of the nuclear protein was performed according to the Pierce cytoplasmic and nuclear extraction kit instructions (Sigma). Samples were run on an SDS-Page gel (BioRad ...
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bioRxiv - Bioengineering 2020Quote: ... Protein was covalently bound to the slide surface via addition of freshly prepared 2.5 mg/mL NaBH4 (Sigma) dissolved in 25 %v/v ethanol in PBS at RT for 5 min ...
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bioRxiv - Biochemistry 2021Quote: ... the protein was purified from the cleared lysate by affinity to Anti-FLAG M2 Affinity gel (Sigma-Aldrich) and eluted in Flag buffer containing 0.5 mg/ml 3xFLAG peptide ...
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bioRxiv - Biochemistry 2021Quote: ... The protein was purified from the cleared lysate by affinity to Anti-FLAG M2 Affinity gel (Sigma-Aldrich) and eluted with pulldown buffer containing 0.1 mg/ml 3xFlag peptide ...
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bioRxiv - Biochemistry 2020Quote: ... Proteins were concentrated using 3 kDa (KrACP, FabD) and 30 kDa (KrKSCLF) Amicon Ultra (Millipore, 15 mL capacity) molecular weight cutoff (MWCO ...
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bioRxiv - Immunology 2020Quote: For immunoprecipitation experiments the Chk1 or GATA3 antibody was incubated with Red Protein G Affinity beads (Sigma-Aldrich) for 1 hour at 4°C on a shaker to allow the antibody to bind to the beads ...
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bioRxiv - Biophysics 2021Quote: ... Next, dissolved membrane proteins (1 nmol) and MSP (50 μL, 1 mg/mL) (MSP1E3D1, Sigma-Aldrich, No. M7074) were added to the lipid suspension and mixed for ∼1 h while rotating in the dark at 4°C ...
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bioRxiv - Neuroscience 2021Quote: ... The supernatant was recovered and the protein concentration was estimated using the Direct Detect® Infrared Spectrophotometer (Millipore). 20 μg proteins were loaded on 4-15% Mini-PROTEAN® TGX Precast Protein Gels Stain Free™ (Bio-Rad® ...
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bioRxiv - Cancer Biology 2021Quote: ... Per sample equal amounts of protein were separated by Bis-Tris-PAGE and transferred to PVDF membranes (Millipore). The membranes were incubated with 5% (w/v ...
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bioRxiv - Cancer Biology 2020Quote: ... The pETDuet-1-His-BAP1-ULD+ASXL2-AB protein complex was expressed in Rosetta 2 (DE3) pLysS (Millipore). The bacteria bearing the desired plasmids were propagated with aeration at 37°C in 1L of 2YT to an A600 absorbance of approximately 0.6 ...
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bioRxiv - Cell Biology 2021Quote: ... Proteins were separated by SDS-PAGE and transferred onto Immobilon-P® PVDF transfer membranes (IPVH00010; EMD Millipore) as previously described (Haynes et al. ...
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bioRxiv - Pathology 2021Quote: ... 2.5-20μg of total protein extracts were separated on acrylamide-SDS gels and transferred to PVDF membranes (Millipore). Specific proteins were detected by using different antibodies listed in supplementary table 1 ...
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bioRxiv - Physiology 2020Quote: ... and purified cell surface proteins were analyzed by western blot using the mαNaVPAN antibody (1:2000, Sigma, #S8809), the anti-transferrin receptor mouse monoclonal antibody (TransR ...
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bioRxiv - Plant Biology 2021Quote: ... Protein complexes were eluted with 100 μl IP buffer supplemented with 200 μg/ml 3xFLAG peptide (Merck Millipore). The eluted protein was then immobilized on anti-c-Myc magnetic beads (Thermo Fisher ...
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bioRxiv - Biochemistry 2020Quote: ... and the sample supernatant was filtered using low-protein binding bottle-top filters (Millipore Express Plus 0.22 µm). This filtered supernatant was supplemented with final concentrations of 2 mM EDTA (stock EDTA 200 mM ...
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bioRxiv - Cell Biology 2021Quote: ... Proteins were separated on SDS-PAGE gels and transferred to PVDF membranes (Cat. #IPFL00010, Millipore, Billerica, MA, USA). The membranes were blocked by 5% fat-free milk diluted in PBS with 0.1% Tween-20 for 40 minutes ...
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bioRxiv - Neuroscience 2021Quote: ... C-reactive protein (CRP) was measured by an ELISA kit (cat. number CYT294, Millipore Sigma, Saint Louis, MO) following the manufacturer’s instructions.
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bioRxiv - Microbiology 2020Quote: ... equal concentrations of cell lysate and media proteins were transfer into 0.45 μm PVDF membrane (Millipore, MA, USA). Further ...
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bioRxiv - Plant Biology 2020Quote: ... Proteins were then analyzed by western blotting using a primary anti-biotin antibody (1:5,000 dilution; Sigma-Aldrich) and an anti-mouse secondary antibody coupled to peroxidase (1:10,000 dilution ...
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bioRxiv - Microbiology 2020Quote: ... 20 μl of the cleared protein sample were mixed with 10 μl of UPS2 spike-in (Sigma-Aldrich) diluted in 250 μl 1.25x protein loading buffer ...
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bioRxiv - Plant Biology 2020Quote: ... Protein extracts were prepared in two volumes (approx.8 ml) of CelLyticP buffer (Sigma-Aldrich, Cat No C2360) with 1 % of a Protease Inhibitor EDTA-free tablet (Roche ...
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bioRxiv - Neuroscience 2021Quote: ... Total protein (30 µg per gel lane) was separated using SDS-PAGE and transferred to PVDF membranes (Millipore). Membranes were blocked with TBST buffer (137m M NaCl ...
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bioRxiv - Neuroscience 2021Quote: ... Homogenate of 50 μg was added to a process control of 800 ng of yeast enolase protein (Sigma) which was then reduced with 20 mM DTT and alkylated with 40 mM IAA ...
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bioRxiv - Plant Biology 2021Quote: Proteins were separated in 10% Bis-Tris polyacrylamide gel using MOPS buffer and transferred to nitrocellulose membrane (Sigma) for immunoblot analysis ...
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bioRxiv - Microbiology 2021Quote: ... The beads were washed with 1x Tris-buffered saline (TBS) three times and proteins were eluted with 55μL of 100μg/mL 3xFLAG peptide (Sigma) in 1x TBS ...
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bioRxiv - Microbiology 2021Quote: ... the separated proteins were transferred to nitrocellulose and probed with commercial primary antibodies recognizing FLAG (Sigma, cat# F3165), HA (Sigma ...
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bioRxiv - Microbiology 2021Quote: ... Input lysates and pulldown proteins were analyzed by western blotting with the anti-FLAG M2 monoclonal antibody (Sigma) and anti-GAPDH monoclonal antibody (Cell Signaling ...
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bioRxiv - Microbiology 2020Quote: ... Protein extracts were separated by SDS-PAGE and analyzed by western blot with anti-FLAG (Sigma-Aldrich #F7425), anti-DNAk (Abcam #ab69617) ...
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bioRxiv - Molecular Biology 2021Quote: ... The Ni-NTA eluted proteins were incubated with washed/pre-conjugated anti-FLAG magnetic beads (Millipore™ M8823) overnight ...
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bioRxiv - Molecular Biology 2021Quote: ... Proteins were eluted twice with equal bead volume (200 μl) of AP buffer supplemented with 3XFLAG peptide (Sigma) at 200 μg/ml at room temperature for 15 minutes on a Nutator.
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bioRxiv - Molecular Biology 2021Quote: ... Chromatin concentrations were normalized among samples and pre-cleared with Protein A/G agarose (Millipore, 100 μL/IP). Following dilution in IP buffer (10 mM Tris-HCl pH = 8.0 ...
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bioRxiv - Cancer Biology 2020Quote: ... 20 μg of protein extract per sample was loaded on acrylamide gel and transferred to PVDF membrane (Millipore). All lysis buffers contained a cocktail of protease inhibitors and phosphatase inhibitors ...
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bioRxiv - Cancer Biology 2020Quote: ... protein samples were diluted 1:1 in EPPS buffer (10 mM EPPS [Sigma-Aldrich, E9502], 1 mM CaCl2), and digested with 1 µg trypsin (Promega ...
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bioRxiv - Cell Biology 2021Quote: ... Proteins were eluted by cleavage of the GST tag by 15 units/ml TEV protease (Sigma T4455-1KU) in PBS buffer overnight at 4 °C ...
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bioRxiv - Neuroscience 2020Quote: ... Protein expression was induced by 1 mM isopropyl β-d-1-thiogalactopyranoside (IPTG, Sigma-Aldrich, Cat. No. I6758) and occurred overnight at room temperature under shaking ...
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bioRxiv - Biophysics 2021Quote: ... The protein solution was then concentrated using a 3 kDa cutoff centrifugal filter unit (Millipore, Burlington, MA, USA) and loaded into a Mono Q 5/50 GL (GE Healthcare Life Sciences ...
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bioRxiv - Biophysics 2020Quote: ... protein purification was accomplished by their affinity to Nickel-NTA agarose beads employing the standard protocol (Novagen, Inc.). There is no evidence that the N-terminal Histidine influences the measurements ...
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bioRxiv - Cell Biology 2021Quote: ... The organoids were transferred to Eppendorf protein LoBind tubes and stained with DAPI (1 μg/mL, Sigma-Aldrich). Finally ...
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bioRxiv - Cell Biology 2021Quote: ... and grown at 37°C to an optical density (600 nm) of ~0.6-0.8 in Super Broth before protein expression was induced with 1 mM IPTG (Sigma) overnight at 18°C ...
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bioRxiv - Cell Biology 2021Quote: ... at a concentration of 1 µg/µl total protein using an ANTI-FLAG M2 Affinity Agarose Gel (Sigma). Beads were centrifuged and washed three times in lysis buffer and eluted and denatured (95 °C ...
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bioRxiv - Cancer Biology 2020Quote: ... Equal amounts of protein were separated by 8-12% SDS-PAGE and transferred to PVDF membranes (Millipore-Merck). Membranes were incubated with primary antibodies ...