Labshake search
Citations for Millipore Sigma :
8801 - 8850 of 10000+ citations for Recombinant Human MSLN Protein Fc tagged since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Immunology 2021Quote: ... the protein elution fractions were pooled and concentrated with 50 kDa molecular weight cutoff ultrafiltration unit (Millipore), then 1 mg of TEV protease was used per 50 mg protein forcleavage during dialyzing to the buffer with 250 mM NaCl ...
-
bioRxiv - Molecular Biology 2020Quote: The fractions representing the monomeric protein were collected and concentrated with Amicon Ultra-centrifugal filters-50K (Millipore). The quality of the final protein product was examined by SDS PAGE ...
-
bioRxiv - Physiology 2020Quote: ... The targeted protein bands were visualized with extended chemiluminescent (ECL) reaction using Immobilon western HRP substrate (Millipore) or by the VECTASTAIN ABC Kit (VECTOR Laboratories ...
-
bioRxiv - Genomics 2020Quote: ... the immunocomplexes were collected with 25 μL of PureProteome™ Protein A Magnetic (PAM) Beads (Millipore, LSKMAGA10) for 2 hours at 4°C with rotation.
-
bioRxiv - Systems Biology 2021Quote: ... protein lysates from the single cells were digested with 2 ng of Trypsin (Sigma cat. nr. T6567), dissolved in 1 μl of 100mM TEAB pH 8.5 containing Benzonase (Sigma cat ...
-
bioRxiv - Cell Biology 2021Quote: ... the solution was incubated with PureProteome™ Protein G Magnetic Beads (Cat. # LSKMAGG10, Millipore, Burlington, MA, USA) and washed according to the manufacturer’s instructions ...
-
bioRxiv - Cell Biology 2021Quote: ... the proteins bound to the beads were eluted by the addition of FLAG peptide (#F3290, Sigma-Aldrich). Proteins in the eluate were separated by SDS-PAGE to detect Tanmp-interacting proteins by Western blotting or by silver staining ...
-
bioRxiv - Molecular Biology 2021Quote: ... the soluble chromatin was precleared with Magna ChIP™ Protein A+G Magnetic Beads (Millipore, 16-663) for 1h and then incubated overnight at 4°C with magnetic FLAG-M2 beads ...
-
bioRxiv - Microbiology 2022Quote: ... followed by nucleic acid digestion with 0.5 Units of Benzonase per 1 µg of protein (Sigma-Aldrich) for 30 min at 37°C ...
-
Deletion or inhibition of PTPRO mitigates diet-induced hepatic steatosis and inflammation in obesitybioRxiv - Immunology 2022Quote: ... proteins were separated with SDS-PAGE gels and transferred to PVDF membranes (Immobilon-P, Merck Millipore, USA). Blotted membranes were reacted with the specific primary antibodies shown in Supplementary Table 1 followed by peroxidase-linked secondary antibodies ...
-
bioRxiv - Cancer Biology 2022Quote: ... The protein was washed and concentrated using 30 kDa Amicon centrifugal filter unit (Millipore Sigma CAT#UFC8030).
-
bioRxiv - Molecular Biology 2022Quote: ... β-mercapto-ethanol 2.8 mM) in presence of thrombin (50 units/10 mg of purified protein; Sigma). The resulting digest was loaded into an 5ml HisTrap column previously equilibrated in buffer B and the flow through was collected ...
-
bioRxiv - Biochemistry 2022Quote: Protein extracts were separated by SDS-PAGE and transferred to polyvinylidene difluoride (PVDF) membranes (Millipore-Merck, Germany). Membranes were blocked with 5% non-fat milk and incubated with a specific anti-biotin antibody overnight at 4°C ...
-
bioRxiv - Cell Biology 2022Quote: ... Immunoprecipitated proteins were eluted with 0.5 mg/mL (diluted in lysis buffer) 3X FLAG peptide (Sigma, F4799) for 30 minutes on a rotator ...
-
bioRxiv - Biochemistry 2022Quote: Biotinylated proteins were digested with 250 ng of trypsin mass spectrometry grade (Sigma-Aldrich, St. Louis, MO) in 50 mM of ammonium bicarbonate (ABC) ...
-
bioRxiv - Cancer Biology 2022Quote: ... Proteins were transferred to nitrocellulose membranes before blocking with 5% milk (Marvel) or bovine serum albumin (Sigma) w/v in PBS-T (0.1% Tween-20 in PBS) ...
-
bioRxiv - Biochemistry 2022Quote: ... bound proteins were eluted with lysis buffer containing 300 μg/ml Flag peptide (Sigma-Aldrich, Cat# F3290) at 4 °C for 45 min ...
-
bioRxiv - Bioengineering 2022Quote: ... supernatant protein was removed by acidification with 5 g L-1 trichloroacetic acid (Supelco from Sigma-Aldrich), incubation for 20 min in room temperature and centrifugation at 20,238 x g for 10 min in a table-top centrifuge (Eppendorf 5424 ...
-
bioRxiv - Biochemistry 2022Quote: ... The protein was concentrated to approximately 3 mg/ml using an ultrafiltration pressure cell (Millipore, Billerica, MA) with a 10-kDa molecular mass cutoff membrane and buffer exchanged with 20 mM HEPES ...
-
bioRxiv - Biochemistry 2022Quote: ... Fractions containing the purified protein were concentrated using a 0.5 ml Amicon Ultra centrifugal filter (Merck Millipore) with a membrane molecular weight cut off 10 kDa.
-
bioRxiv - Biochemistry 2022Quote: ... Protein-containing fractions were pooled and concentrated using centrifugal ultra-filtration devices (10 kDa cut-off; Millipore). Protein concentration was determined using a Bradford assay with a standard curve calibration with BSA ...
-
bioRxiv - Biophysics 2022Quote: ... the expression of the protein was induced with 0.5 mM isopropyl β-D-1-thiogalactopyranoside (IPTG – Sigma) and the flask was placed at 37°C ...
-
bioRxiv - Cell Biology 2022Quote: ... Protein was reduced with a final concentration of 10 mM Tris(2 - carboxyethyl)phosphine hydrochloride (TCEP) (Sigma) and alkylated with 40 mM 2-chloroacetamide (CAA ...
-
bioRxiv - Cell Biology 2022Quote: ... LATS1 kinase was immunoprecipitated from 500 µg of protein lysates and subject to Oxyblot assay (#S7150, Millipore) according to manufacturer’s instructions.
-
bioRxiv - Developmental Biology 2022Quote: Separated proteins were wet transferred onto 0.45mm pore-sized PVDF membranes (Immobilon®-FL PVDF membrane, Millipore) for 2 hours at 250mA and 4°C using cold transfer buffer (1.5x NuPAGE transfer buffer (Thermo Fisher Scientific) ...
-
bioRxiv - Cell Biology 2022Quote: ... The presence of proteins in samples was detected using Immobilon Western Chemiluminescent HRP Substrate (Sigma-Aldrich, France). Acquisition was performed on a ChemiDoc Imaging System (Biorad ...
-
bioRxiv - Immunology 2022Quote: Cell extracts (1 mg protein) were treated for 15 min at ambient temperature with benzonase (Merck-Millipore) and pre-cleared by incubation for 30 min at 4°C with 20 µl of protein A/G-Sepharose beads ...
-
bioRxiv - Immunology 2022Quote: ... Equivalent amounts of protein were separated by SDS-PAGE and transferred to Immobilon-P PVDF membrane (Millipore). Membranes were incubated with antibodies per manufacturer’s suggestions ...
-
SMDT1 variants impair EMRE-mediated mitochondrial calcium uptake in patients with muscle involvementbioRxiv - Genetics 2022Quote: ... to 20 μg of mitochondrial protein and run with Tris-MOPS running buffer (#PCG3003; Sigma-Aldrich/Merck) on a 4-20% precast Trupage gel (#PCG2012-10EA ...
-
bioRxiv - Immunology 2022Quote: ... Purified proteins were concentrated using a 100 kDa and 30 kDa cutoff concentrator (EMD Millipore, Burlington, MA), respectively ...
-
bioRxiv - Microbiology 2022Quote: ... Proteins were concentrated where necessary using Amicon filter devices with appropriate molecular weight cut-off (Millipore; Merck).
-
bioRxiv - Microbiology 2022Quote: ... The supernatant was filtered through a 0.45 μm low protein binding durapore membrane (Merck Millipore Ltd., Ireland). 20 mL of the filtrate was then ultracentrifuged at 150,000 × g at 4°C for 3 h (Beckman Coulter ...
-
bioRxiv - Plant Biology 2022Quote: ... proteins were electroblotted from 10% acrylamide gel to nitrocellulose membrane (Immobilon-P, MILLIPORE Corporation, Bedford, MA, USA) after the separation of SDS-PAGE ...
-
bioRxiv - Molecular Biology 2023Quote: ... magnetic protein A/G beads were prepared with 5 µg of rabbit anti-AGO2 (Millipore, #03-110) or normal rabbit IgG antibodies (from the Magna RIP kit) ...
-
bioRxiv - Physiology 2023Quote: Biosynthetic labelling of newly synthesised proteins was achieved by oral consumption of deuterium oxide (Sigma-Aldrich, UK), consistent with our previous work (Camera et al. ...
-
bioRxiv - Plant Biology 2022Quote: ... and transferred to a PVDF membrane followed by probe with Phos-tag BTL-111 according to the manufacturer’s instructions (www.wako-chem.co.jp) to detect protein phosphorylated levels and anti-FLAG®M2-Peroxidase (HRP) (Sigma) antibody to detect the total amount of immunoprecipitated TMK1-FLAG.
-
bioRxiv - Neuroscience 2023Quote: ... or growth associated protein-43 (GAP43; rabbit anti-GAP43; 1:1000; #AB5220, AB_2107282; Millipore, Billerica, MA, USA). Subsequently ...
-
bioRxiv - Molecular Biology 2022Quote: ... the proteins were eluted by 2 mM biotin in buffer W and concentrated with 100kDa Amicon (Millipore). The concentration of dtSARM1 was determined by BCA assay (Thermo Fisher).
-
bioRxiv - Molecular Biology 2022Quote: ... The ORF1p-FLAG protein complex bound was eluted using 200 μg/mL of 3xFLAG peptide (Sigma-Aldrich) in the Lysis150 buffer containing 0.2 mM PMSF and 1x cOmplete EDTA-free protease inhibitor cocktail by incubation at 4°C for 1 hour with gentle rotation ...
-
bioRxiv - Molecular Biology 2022Quote: ... SDS-polyacrylamide gel (10%) was cast in the presence of 0.5 mg/ml myelin basic protein (Sigma) within the gel ...
-
bioRxiv - Molecular Biology 2022Quote: ... The purified protein was concentrated using an Amicon Ultra 100 K filter (Merck Millipore, Billerica, Massachusetts, USA) before further purification using Superdex 200 gel filtration (GE Healthcare ...
-
bioRxiv - Molecular Biology 2022Quote: RNA immunoprecipitation (RIP) was performed using the Magna RIP RNA-Binding Protein Immunoprecipitation Kit (Millipore, Burlington, MA). Briefly ...
-
bioRxiv - Molecular Biology 2022Quote: ... The resulting eluate was collected and used for total protein isolation using Trichloroacetic acid-TCA (Sigma, T0699). The eluted proteins were then analysed by Coomassie staining using Brilliant Blue G colloidal concentrate (Sigma Aldrich ...
-
bioRxiv - Cancer Biology 2022Quote: ... Then T24 chromatin’s DNA and proteins were separated by adding 0.5 µl Benzonase (Sigma Aldrich, E8263-5KU) and vortexing ...
-
bioRxiv - Plant Biology 2022Quote: ... Proteins were separated by SDS acrylamide gel electrophoresis and transferred to IMMOBILON-FL 26 PVDF membrane (Millipore) probed with the indicated antibodies and visualized either by chemiluminescence (according to the manufacturer’s instructions ...
-
bioRxiv - Cancer Biology 2022Quote: ... Protein elution was carried out under native conditions by competition with commercially available 3× FLAG peptide (Sigma). Eluted proteins separated in 4-20% polyacrylamide gels in denatured conditions were visualized for purity after Coomassie blue (Bio-Rad ...
-
bioRxiv - Neuroscience 2024Quote: ... centrifuged for 15 min at 20 000 g and protein concentration was assessed using Bradford assay (Sigma). 50 µg of protein for each sample was transferred in fresh tubes ...
-
bioRxiv - Systems Biology 2024Quote: ... Proteins were detected by incubation in BCIP/NBT AP substrate buffer (Sigma-Aldrich, St. Louis, MO, USA).
-
bioRxiv - Microbiology 2024Quote: ... was used as second antibody and proteins on the membrane were visualized using BCIP/NBT substrate (Sigma). When deer plasma specimens were used as primary antibody ...
-
bioRxiv - Plant Biology 2024Quote: ... Labelled proteins were biotinylated with 30 μM azide-PEG3-biotin (Sigma-Aldrich, 2 mM stock in DMSO) using click chemistry as described above ...