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Citations for Millipore Sigma :
8751 - 8800 of 10000+ citations for Recombinant Human MSLN Protein Fc tagged since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2020Quote: ... Identical amounts of protein samples were subjected to SDS-PAGE and transferred to Immobilon-FL membrane (Millipore). Immunoprecipitated samples were subjected to SDS-PAGE and transferred to Immobilon-FL membrane (Millipore) ...
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bioRxiv - Microbiology 2020Quote: ... 20-30 μg protein was separated by 10% SDS-PAGE and transferred to PVDF membranes (Millipore, USA). After being blocked with 5% non-fat milk for 1 hour ...
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bioRxiv - Molecular Biology 2020Quote: ... Protein concentrations of wholecell lysates were determined by the QuantiPro™ BCA Assay Kit (Sigma-Aldrich, QPBCA). The proteins were then electrophoresed in SDS-PAGE gels to separate by molecular weight and transferred to polyvinylidene difluoride membranes (Millipore ...
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bioRxiv - Cell Biology 2019Quote: ... After the run was completed the proteins were stained with Coomassie blue stain (Sigma Cat No-G1041). The gel band from IgG Lane ...
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bioRxiv - Neuroscience 2019Quote: ... Eya1 protein was concentrated to approximately 2 mg/ml using an Amicon Ultra concentrator (30 MWCO, Millipore) and further purified by size-exclusion chromatography on a Superdex 200 Increase 10/300 column (GE Lifescience ...
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bioRxiv - Molecular Biology 2020Quote: ... The protein complexes were purified using the FLAG-affinity purification approach in the presence of benzonase (Sigma), and analyzed by SDS-PAGE and silver staining before subjected to Mass Spectrometry.
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bioRxiv - Biochemistry 2020Quote: ... Equal amounts of protein (20ug) were loaded onto a 10% polyacrylamide gel and 0.45μM nitrocellulose membrane (Millipore, Billerica ...
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bioRxiv - Bioengineering 2021Quote: ... 15N-labeled protein for NMR analysis utilized M9 media with 13C glucose and 15N ammonium salts (Sigma) as the sole carbon and nitrogen sources ...
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bioRxiv - Molecular Biology 2020Quote: ... 10 ug protein/sample was diluted in 1X LDS Sample Buffer and 50 mM DTT (Sigma-Aldrich), then incubated for 10 minutes at 70°C ...
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bioRxiv - Developmental Biology 2019Quote: ... The target protein levels were normalized to the endogenous control (GAPDH, mouse, Millipore Sigma, MAB374, 1/500). The membranes were scanned on the LI-COR Odyssey CLx imaging system.
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bioRxiv - Microbiology 2019Quote: ... Protein lysates were separated by SDS-PAGE and then transferred onto polyvinylidene fluoride membranes (PVDF, Merck Millipore) for immunoblot analysis ...
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bioRxiv - Biochemistry 2020Quote: ... Protein complex was incubated overnight on ice and then concentrated onto 30 kDa MWCO Amicon concentrator (Millipore) until protein concentration reached 11 mg/ml ...
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Transient upregulation of translational efficiency in prodromal Tg2576 mice precipitates AD symptomsbioRxiv - Neuroscience 2019Quote: ... and 50 μg of total protein were loaded on 12% acrylamide gel for determination of APP (Sigma), Amyloid-β (Aβ ...
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bioRxiv - Genetics 2021Quote: ... The cell lysate was pre-cleared with 30 μ L Protein A agarose beads (Millipore, 16-156) for 1 hour at 4°C and then incubated overnight on a rotor with antibody coated beads ...
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bioRxiv - Cell Biology 2020Quote: ... Proteins were separated on 10% SDS-PAGE gels followed by transfer to polyvinylidene difluoride Immobilon membranes (Millipore). Phospho-Erk was detected using a phospho-Erk antibody (clone 4695P ...
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bioRxiv - Neuroscience 2021Quote: ... PLGA) nanoparticles (NP) loaded with the protein (in the control condition bovine serum albumin, BSA, Sigma-Aldrich). The NPs were prepared according to the protocol described by Chaturvedi et al.29 ...
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bioRxiv - Biochemistry 2021Quote: ... Elution fractions containing the protein were pooled and concentrated (Amicon Ultra 100 kDa cut off, Millipore Sigma) for gel filtration ...
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bioRxiv - Neuroscience 2021Quote: ... the total protein concentration of each supernatant was measured using a Direct Detect Infrared Spectrometer (Millipore Sigma). Cytokines/chemokines were then quantified by multiplex immunoassay using a high sensitivity mouse multiplex magnetic bead kit (Millipore Sigma ...
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bioRxiv - Neuroscience 2020Quote: ... The protein extracts were run on a 5%–12% gel and transferred to nitrocellulose (NC) membranes (Millipore). After blocking in a milk solution (5% powdered milk in PBS ...
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bioRxiv - Cell Biology 2020Quote: ... equal amounts of protein were subjected to SDS polyacrylamide gel electrophoresis and transferred to PVDF membrane (Millipore) by wet transfer ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... Protein was quantified according to the Coomassie blue method (BRADFORD, 1976) using bovine serum albumin (Sigma-Aldrich) as a standard ...
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bioRxiv - Neuroscience 2020Quote: ... Interactions between CB1 and cytoskeleton proteins were detected using the Duolink in situ PLA detection Kit (Sigma) following the instructions of the supplier ...
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bioRxiv - Biochemistry 2021Quote: Protein carbonyl levels in yeast whole cell lysates were assessed using a colorimetric assay (Sigma-Aldrich, MAK094). WT ...
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bioRxiv - Biochemistry 2021Quote: ... protein lysates from the single cells were digested with 2 ng of Trypsin (Sigma cat. nr. T6567), dissolved in 1 μl of 100 mM TEAB pH 8.5 containing Benzonase (Sigma cat ...
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bioRxiv - Biochemistry 2021Quote: ... The separated proteins were transferred by electrophoresis to polyvinylidene difluoride transfer membranes (Immobilon-P, Millipore, MA, USA). The membranes were treated with diluted affinity-purified anti-p-eIF2α (p-eIF2α ...
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bioRxiv - Synthetic Biology 2021Quote: ... The enzyme and the expressed proteins were inactivated by adding 15mM EDTA (Catalog No. E9884, Sigma-Aldrich) at 75°C for 10 minutes (T100 Thermal Cycler ...
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bioRxiv - Genetics 2020Quote: ... Equal protein loading was confirmed by probing with mouse-anti-β-actin antibody (1:10,000; Sigma, A5441).
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Chemoproteomics of microbiota metabolites reveals small-molecule agonists for orphan receptor GPRC5AbioRxiv - Biochemistry 2021Quote: ... and the soluble proteins were incubated with EZview red anti-HA or anti-Flag affinity gel (Sigma) for 3 h at 4 °C ...
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bioRxiv - Biochemistry 2021Quote: ... was added into 200 µL cell lysate precleared by 20 µL Protein A magnetic beads (EMD Millipore) and incubated overnight at 4°C ...
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bioRxiv - Biophysics 2021Quote: ... LCP) sample was obtained by mixing 40 μl of GtACR1 protein with 60 μl monoolein (MO) (Sigma, St ...
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bioRxiv - Neuroscience 2020Quote: ... The harvested protein was concentrated by Amicon Ultra-15 centrifugal filter units (Millipore Sigma, Burlington, MA, USA), filtered through a 0.22 µm filter (E&K Scientific ...
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bioRxiv - Cancer Biology 2021Quote: ... Protein lysates were incubated overnight with 5 µL of Flag or V5 antibody-conjugated beads (Sigma-Aldrich) or with 1 µl of p50 antibody together with 20 µl of Protein A magnetic beads (Life Technologies) ...
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bioRxiv - Biophysics 2020Quote: ... The proteins were separated by SDS-PAGE and then transferred to a PVDF membrane (Millipore, Burlington, Massachusetts). Membranes were stained with 1:1000 dilution of Anti-SARS-CoV SΔ10 antibody ([1A9] ...
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bioRxiv - Cancer Biology 2021Quote: ... Proteins were run in SDS-PAGE and transferred to Immobilon Polyscreen PVDF transfer membranes (#IPVH00010, Merck Millipore) or Amersham Protan nitrocellulose membranes (#10600001 GE Healthcare Life Sciences) ...
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bioRxiv - Microbiology 2021Quote: ... and proteins were eluted in 50 mM Tris-HCl (pH 8) containing 10 mM reduced glutathione (Sigma). Glutathione was removed using a desalting Zeba column following manufacturer instructions (ThermoFisher Scientific).
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bioRxiv - Cell Biology 2021Quote: ... The separated proteins were then transferred to the PVDF membranes (0.45 µm Immobilon®-E; Merck Millipore). Membranes were blocked either with 2.5% nonfat milk in TBST (50 mM Tris ...
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bioRxiv - Cell Biology 2020Quote: Immunodepletion of Cyclin A from Xenopus embryo extracts was carried out using AffiPrep Protein A beads (Sigma) conjugated with the Xenopus laevis anti-Cyclin A1 (a gift from Daniel Fisher ...
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bioRxiv - Cell Biology 2020Quote: ... After eluting and concentrating the cleaved protein in Amicon Ultra-15 Centrifugal Filters 50K MWCO (Merck Millipore), size exclusion chromatography (SEC ...
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bioRxiv - Cell Biology 2020Quote: The indicated proteins were loaded to SDS-PAGE gel and transferred to methanol-activated PVDF membranes (Millipore), which were blocked in 5% non-fat milk containing 0.2% Tween for 1 h at room temperature (RT) ...
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bioRxiv - Cell Biology 2020Quote: ... Purification of both protein constructs was similar: plasmid was transformed into the Rosetta2 strain of E.coli (Novagen). Two liters of culture were grown in terrific broth for 8-12h at 37°C (until the OD600 ~2) ...
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bioRxiv - Cell Biology 2019Quote: ... Aurora B (0.01 µg/µl) and myelin basic protein (MBP; 2.5 µg) (Sigma-Aldrich and Biaffin GmbH) or one of the three Mid1p domains (“N-term” ...
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bioRxiv - Evolutionary Biology 2020Quote: ... we employed antibodies against RNA-Binding Protein RBFOX3 (aka NeuN; mouse anti-NeuN, Alexa488-conjugated, MAB377X, Millipore) that is expressed in all neuronal nuclei ...
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bioRxiv - Genetics 2021Quote: Total protein was isolated using RIPA lysis buffer (Biocolors, China) with a protease inhibitor cocktail (Sigma, USA). All protein samples were subjected to concentration determination and immunoblotting assay with the indicated antibodies ...
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bioRxiv - Microbiology 2021Quote: ... for 5h and then Protein G-DAF complexes were crosslinked using bis(sulfosuccinimidyl)suberate (BS3) (Sigma, S5799). Protein from total cell extracts (100 µg ...
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bioRxiv - Immunology 2020Quote: ... coli BL21(DE3) strain and protein expression was induced in Overnight Express(tm) Instant TB Medium (Novagen) at 37°C ...
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bioRxiv - Microbiology 2021Quote: ... This magnetic resin was generated by incubating Dynabead Protein G (ThermoScientific) with anti-FLAG antibodies (Sigma Aldrich) at room temperature followed by washing ...
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bioRxiv - Immunology 2020Quote: ... Detection of the SARS CoV-2 spike protein was achieved by incubation with an anti-rabbit (Sigma) secondary antibody ...
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bioRxiv - Molecular Biology 2021Quote: ... (Table 2) and the immune complexes were captured with protein A agarose fast-flow beads (Sigma-Aldrich) for 1h at RT ...
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bioRxiv - Microbiology 2020Quote: ... Proteins contained in BALF and TS were concentrated 5x using an Amicon Ultra column 100kDa (Merck Millipore) and 20 min centrifugation at 4000xg and samples were stored at −80°C ...
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bioRxiv - Microbiology 2021Quote: ... The precipitated protein and input control were detected by the Western blotting with anti-Flag (Sigma, A8592) or anti-GFP (Abcam ...