Labshake search
Citations for Millipore Sigma :
701 - 750 of 2519 citations for ssc mir 376c RT PCR Primer Set since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Evolutionary Biology 2020Quote: ... The biotin-bound capture primers (Sigma-Aldrich, Gillingham, UK) were attached to biotinylated magnetic beads (Promega ...
-
bioRxiv - Immunology 2020Quote: ... The following primers were purchased for use from Sigma: mAqp9 fwd 5′-TGGTGTCTACCATGTTCCTCC-3′ ...
-
bioRxiv - Developmental Biology 2022Quote: ... with 200 nM Forward and Reverse primers (Sigma-Aldrich; designed using Primer3 software (Untergasser et al. ...
-
bioRxiv - Physiology 2022Quote: ... 1.2 µL of KiCqStart SYBR Green Predesigned Primers (Sigma) at a concentration of 10 nM ...
-
bioRxiv - Cancer Biology 2023Quote: ... Primers were from Sigma-Aldrich (St. Louis, MO, USA) and were directed against exon-exon boundaries for RT-qPCR or directed against intron-exon boundaries or introns for DNA-qPCR (Table S1) ...
-
bioRxiv - Bioengineering 2023Quote: The primers (M1-M4) were obtained from Sigma Aldrich and the sequence is mentioned in Table 1 ...
-
bioRxiv - Developmental Biology 2023Quote: ... and KiCqStart SYBR green primers (Sigma Merck, Table S1). Obtained Ct values were used for relative quantification of mRNA expression using the ddCt method ...
-
bioRxiv - Immunology 2023Quote: ... All other primers were designed and obtained from Sigma and are summarized in Supplemental Tables 2 and 3 ...
-
bioRxiv - Biophysics 2023Quote: ... 0.6 μL GAPDH and β-actin primers (Sigma Aldrich), 6.4 μL H2O and 5 μL cDNA ...
-
bioRxiv - Microbiology 2024Quote: ... Oligonucleotide primers (Table S7) were synthesized by Sigma-Aldrich. Vector pDS132 was used to construct plasmids for in-frame deletion ...
-
bioRxiv - Immunology 2023Quote: ... and primers supplied by Sigma Aldrich (see table 1) were used unless otherwise stated ...
-
bioRxiv - Cell Biology 2023Quote: ... and random hexamer primers (Sigma-Aldrich, cat.no. 11034731001-2MG). Gene expression was determined by qPCR using InnuMIX qPCR DSGreen Standard (Analytik Jena ...
-
bioRxiv - Cell Biology 2024Quote: ... and specifically designed primers (Eurogentec, Liège, Belgium; Millipore Sigma) listed in Table 1 were used ...
-
bioRxiv - Microbiology 2022Quote: ... and primary antibodies in hybridization buffer (10% deionized formamide, 10% dextran sulfate [Sigma-Aldrich], 2 mM vanadyl ribonucleoside complexes [VRC, Sigma-Aldrich] in 2x SSC). FISH probes were added at a final concentration of 250 nM ...
-
bioRxiv - Cell Biology 2020Quote: ... miRNA-147-3p or miR-298-5p mimics (Dharmacon) and incubated for 72 hours in DMEM/OptiMEM medium (Sigma-Aldrich/Thermo Fisher Scientific) prior lysis.
-
bioRxiv - Cancer Biology 2019Quote: ... for primary cells miR-E shRNA viruses were concentrated to increase transduction efficiency using Centriprep Centrifugal filter YM-50 (Millipore, Billerica, MA, USA).
-
bioRxiv - Developmental Biology 2021Quote: ... RT-qPCR was performed using SYBR Green Mastermix (Sigma) and primers detailed in Table 3.
-
bioRxiv - Molecular Biology 2020Quote: ... Cells were blocked at RT with 5% BSA (Sigma) dissolved into PBS ...
-
bioRxiv - Microbiology 2021Quote: ... and 2 µL ThermaSTOP RT (Sigma Aldrich, #TSTOPRT-250). Mix 1 was heated to 65°C for 5 min and then snap-chilled on ice for at least 2 min ...
-
bioRxiv - Pharmacology and Toxicology 2021Quote: ... and Hoechst 33342 (1μg/mL; 5 min; RT; Sigma).
-
bioRxiv - Molecular Biology 2022Quote: RNA was isolated by using RNAZol-RT reagent (Sigma) from exponentially growing MtbH37Rv/Msm mc2155 and its Rho CKD strain ...
-
bioRxiv - Developmental Biology 2023Quote: ... overnight at RT and included in paraffin (Sigma-Aldrich) following standard procedures ...
-
bioRxiv - Cell Biology 2023Quote: ... at RT and fixed with 4% paraformaldehyde (Sigma Aldrich) in CB for 20 min (RT) ...
-
bioRxiv - Neuroscience 2020Quote: ... Followed by an incubation in darkens with SIGMA FAST 3,3’-Diaminobenzidine Tablet SETS (SIGMA) for 75 min at RT ...
-
bioRxiv - Biochemistry 2022Quote: ... a set of CasMam oligonucleotides was designed and synthesized (Sigma-Aldrich/Merck, desalt purification). The oligonucleotides contained the optimized linker sequences (α/β/γ/δ/ε/ω ...
-
bioRxiv - Plant Biology 2020Quote: ... After removal of DNA using the on-column DNase digestion Set (Sigma-Aldrich Australia), RNA quality and quantity was assessed on a TapeStation 2200 system (Agilent) ...
-
bioRxiv - Genomics 2019Quote: ... frozen tissues were placed into a 2 ml KIMBLE Dounce tissue grinder set (Sigma) with homogenization buffer consisted of 20 mM Tris pH 8.0 (Thermo Fisher Scientific) ...
-
bioRxiv - Developmental Biology 2019Quote: ... A standard set of phosphopeptides (4 pmol/peptide sample, MS PhosphoMix1 Light, Sigma, MSPL1) were spiked into each sample ...
-
bioRxiv - Neuroscience 2022Quote: ... and 1:200 Protease Inhibitor Cocktail Set III (all chemicals purchased from Sigma Aldrich)) ...
-
bioRxiv - Cell Biology 2022Quote: ... supplemented with protease inhibitor cocktail Set I-Calbiochem 1:100 (Cat# 539131, Merck Millipore) and phosphatase inhibitors ...
-
bioRxiv - Cell Biology 2022Quote: ... supplemented with protease inhibitor cocktail Set I-Calbiochem 1:100 (Cat# 539131, Merck Millipore) and phosphatase inhibitors ...
-
bioRxiv - Cell Biology 2022Quote: ... Another set of clean coverslips was treated with poly-L-lysine solution (Sigma-Aldrich) for 45 min at 37ºC in a humid chamber to activate the surface ...
-
bioRxiv - Plant Biology 2024Quote: ... RNA was immediately extracted from pooled samples after grinding using a Spectrum™ Plant Total RNA Kit from Sigma-Aldrich (STRN250) and an On-Column DNase I Digestion Set from Sigma-Aldrich (DNASE70-1SET) to remove DNA ...
-
bioRxiv - Molecular Biology 2019Quote: ... PCR was performed in a 10-μl reaction composed of 0.3 μl of a 10 μM dilution of forward and reverse primers (0.6 μl in total; primers were synthesized by Sigma-Aldrich, Saint Louis, MO, USA), 0.125 μl dNTP mix (concentration of each nucleotide was 10 mM ...
-
bioRxiv - Cell Biology 2020Quote: ... All primers were synthesized by Sigma-Aldrich (St. Louis, USA) and purified by HPLC ...
-
bioRxiv - Molecular Biology 2021Quote: ... Predesigned KiCqStart SYBR Green optimized primers from Sigma-Aldrich (KSPQ12012) were used for RT-qPCR.
-
bioRxiv - Developmental Biology 2020Quote: ... The primers were ordered from Sigma (0.05 μmoles and desalted) with a length varying between 90-150bps (the sequence is given in supplemental table 4) ...
-
bioRxiv - Molecular Biology 2019Quote: ... Long homology arm introducing primers were ordered from Millipore Sigma, PAAG.
-
bioRxiv - Neuroscience 2020Quote: ... primer pairs were obtained from a commercial vendor (Sigma-Aldrich) and primer specificity was tested with adult rat whole-brain cDNA ...
-
bioRxiv - Synthetic Biology 2021Quote: ... Primers used in this work were provided by Sigma-Aldrich Co (St ...
-
bioRxiv - Microbiology 2020Quote: ... Reverse Primer 5’-TGGTTGAGCACAGGGTACTT-3’] were synthesized by Sigma-Aldrich, USA ...
-
bioRxiv - Cancer Biology 2020Quote: ... and non-universal primers (Thermofisher Scientific, Sigma-Aldrich or Eurofins) designed using Primer3web online tool ...
-
bioRxiv - Microbiology 2022Quote: ... Custom primers in this study were made by Sigma-Aldrich.
-
bioRxiv - Neuroscience 2021Quote: Mastermix was prepared using forward and reverse primers (Sigma, UK) and where possible a labelled probe for each gene (Table 1) ...
-
bioRxiv - Plant Biology 2021Quote: ... 0.25 μM each of RamF6 and RamR6 primers (Sigma, Australia), 0.15 μM of molecular beacon Ram6 (Sigma ...
-
bioRxiv - Biochemistry 2022Quote: ... All primers in this study were obtained from Sigma-Aldrich, and the restriction sites are underlined ...
-
bioRxiv - Developmental Biology 2019Quote: ... All primers were synthesized by Sigma-Aldrich (St Louis, MO), and gene expression was normalized to 18s rRNA by the ΔΔCT method.
-
bioRxiv - Physiology 2021Quote: ... Telomeric forward and reverse primer sequences (Sigma, The Woodlands, TX) were (5⍰→3⍰ ...
-
bioRxiv - Microbiology 2021Quote: ... in a thermocycler with desalted oligonucleotide primers (Sigma-Aldrich, UK) according to the manufacturer’s protocol ...
-
bioRxiv - Genetics 2020Quote: ... Single gRNA oligos and primers were purchased from Sigma Aldrich, and cloned into the NotI site of plasmids ...