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Citations for Millipore Sigma :
801 - 850 of 2519 citations for ssc mir 376c RT PCR Primer Set since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2022Quote: ... using FAM-labelled forward primers (Sigma Aldrich; St. Louis, MO, USA). Microsatellite profiles were analysed with Geneious 8.0.5 and checked with MICROCHECKER v2.2.3 (http://www.microchecker.hull.ac.uk/ ...
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bioRxiv - Microbiology 2023Quote: ... and desalted primers (Sigma-Aldrich, St. Louis, MO, Table S 3), with an initial 10-minute incubation at 95 °C to release E ...
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bioRxiv - Genomics 2023Quote: ... 0.5 μl of 2nd primer UMI8 (Table S2, 100μM, Sigma-Aldrich), containing 8 mer UMI sequence and 1.3 μl of DMSO ...
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bioRxiv - Cancer Biology 2023Quote: ... Primer sequences used to amplify genes were purchased from Sigma-Aldrich and are listed in Supplementary Table S3.
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bioRxiv - Cell Biology 2020Quote: ... for 30 min at RT followed by 1 h incubation at RT with rabbit anti-FLAG antibody (1:500, Sigma-Aldrich, St. Louis, MO, USA) and mouse anti-SEC31A antibody (1:500 ...
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bioRxiv - Immunology 2021Quote: ... USA) with the antibody extraction buffer prepared by adding the protease inhibitor cocktail set I (Millipore) to 2x PBS ...
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bioRxiv - Cell Biology 2022Quote: ... RNA was treated with DNAse in solution using On-Column DNase I Digestion Set (Sigma-Aldrich) and maintained with Ribosafe RNAse Inhibitor (Bioline) ...
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bioRxiv - Cell Biology 2019Quote: ... Blots were labelled with the appropriate set of antibodies: primary rat anti-HA (1:500, Sigma), mouse anti-Myc (1:1000 ...
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bioRxiv - Plant Biology 2020Quote: ... Genomic DNA contamination was eliminated by using the On-Column DNase I Digestion Set (Sigma-Aldrich, St ...
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bioRxiv - Cell Biology 2021Quote: Total RNA was extracted from three sets of 10 Drosophila heads using TRI-Reagent (T9424, Sigma) and RNA was reverse transcribed using an iScript cDNA Synthesis Kit (Bio-Rad ...
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bioRxiv - Genetics 2022Quote: ... On-column DNase digestion was performed as described with On-Column DNaseI Digestion Set (Sigma Aldrich). For the 7 DAP reference ...
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bioRxiv - Developmental Biology 2022Quote: ... A DNase removal step was included using an on-Column DNase 1 Digestion Set (Sigma-Aldrich). The total RNA was quantified using a Nanodrop-ND1000 Spectrophotometer (Thermo Scientific ...
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bioRxiv - Neuroscience 2020Quote: ... a subset of slices were mounted in a hard-set mounting medium (2.5% DABCO (D27802; Sigma), 10% polyvinyl alcohol (P8136 ...
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bioRxiv - Neuroscience 2020Quote: ... 1 mM EDTA and 1% Nonidet P-40) supplemented with protease inhibitor (Cocktail Set III; Millipore) for 10 mins on ice ...
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bioRxiv - Cell Biology 2021Quote: ... 10 mM BME) and 4 μl of protease inhibitor (Protease Inhibitor Cocktail Set IV, EMD Millipore) was added to every gram of cell powder ...
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bioRxiv - Neuroscience 2022Quote: ... reactions were set up in assay buffer containing 20 mM sodium phosphate (pH 6.9; Sigma-Aldrich), 300 mM NaCl (Sigma-Aldrich) ...
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bioRxiv - Bioengineering 2023Quote: Assays were set up manually in white flat bottom 96-well plates (BRANDplates®; Sigma-Aldrich) at room temperature ...
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bioRxiv - Neuroscience 2023Quote: ... DAB (Sigma Fast 3,3 Diaminobenzidine Tablet Set, D-4418 (Sigma-Aldrich Corp, St. Louis, MO, USA)) staining was used as a counterstain.
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bioRxiv - Microbiology 2023Quote: ... The same set of experiments were repeated with additional application of Protease Inhibitor Cocktail (#P9599, Sigma). To compare the effect of protein digestion in mucilage from D ...
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bioRxiv - Molecular Biology 2024Quote: ... The column calibration was performed using a set of gel filtration markers (Sigma-Aldrich cat. # MWGF1000), including Blue Dextran 2000 ...
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bioRxiv - Developmental Biology 2019Quote: ... in 50 mM ammonium bicarbonate (09830, Sigma-Aldrich; 30 min, RT), and alkylated in 50mM iodoacetamide (90034 ...
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bioRxiv - Neuroscience 2020Quote: ... slices were permeabilized at RT in 0.5% Triton X-100 (Sigma), followed by 1 hr RT blocking in 2% BSA 0.5% Triton X-100 and overnight incubation with primary antibody.
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bioRxiv - Cell Biology 2020Quote: ... were incubated 10 minutes at RT with Bind-Silane (Sigma-Aldrich) dissolved in ethanol absolute (PanReac ...
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bioRxiv - Microbiology 2022Quote: ... RNA was extracted from A549 cells using RNAzol RT (Sigma-Aldrich) following the manufacturer protocol ...
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bioRxiv - Biochemistry 2022Quote: ... Standard curves were generated using recombinant HIV-1 RT (Merck Millipore). The relative viral quantities were calculated based on the standard curve generated using QuantStudio-7 systems software (Applied Biosystems).
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bioRxiv - Biophysics 2021Quote: ... cells were incubated 10 min at RT with 1% glycine (Sigma). Cells were permeabilized by 0.1% saponin (Rectapur Prolabo ...
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bioRxiv - Microbiology 2019Quote: ... sodium azide (50 µg/ml, RT for 30 min, Sigma #S2002) for ATPase inhibition or chloramphenicol (30 µg/ml ...
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bioRxiv - Microbiology 2021Quote: ... RT SigmaFast O-phenylenediamine (OPD) substrate (P9187, Sigma, St. Louis, MO) was added to the wells after six additional washes to develop the colorimetric reaction ...
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bioRxiv - Cancer Biology 2021Quote: Telomerase activity was confirmed using the TRAPeze® RT kit (Sigma) according to manufacturer’s instructions ...
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bioRxiv - Genomics 2020Quote: ... carefully layered on an RT Ficoll gradient (Sigma HISTOPAQUE®-1119), and centrifuged at 400 x g for 30 minutes at room temperature ...
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bioRxiv - Microbiology 2020Quote: ... RT SigmaFast O-phenylenediamine (OPD) substrate (P9187, Sigma, St. Louis, MO) was added to the wells after six additional washes to develop the colorimetric reaction ...
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bioRxiv - Neuroscience 2022Quote: ... for 1 h at RT and with DAPI (1/20,000; Sigma) for 5 min at RT ...
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bioRxiv - Immunology 2022Quote: ... for 2 hours at RT and then mounted with Fluoromount (Sigma). Primary Antibodies used were rabbit anti-ACE2 (1:500 ...
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bioRxiv - Bioengineering 2022Quote: ... pressure-sensitive foils (Z734438, ThermalSeal RTS™ Sealing Films, Sigma-Aldrich) with holes for air exchange and to enable access to the reservoirs for medium exchange ...
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bioRxiv - Plant Biology 2023Quote: Total RNA was isolated using RNAzol® RT (Sigma-Aldrich, R4533) from three biological replicates of WT ...
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bioRxiv - Neuroscience 2023Quote: ... all slices were incubated 20 min at RT with DAPI (Sigma). Finally ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... for 5 min at RT and 200 µL chloroform (Sigma-Aldrich) was added ...
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bioRxiv - Developmental Biology 2023Quote: ... 1 hr at RT in 0.1 % Calcofluor White (910090, Sigma-Aldrich), and (3 ...
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bioRxiv - Cancer Biology 2023Quote: Telomerase activity was confirmed using the TRAPeze® RT kit (Sigma) (13–16) ...
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bioRxiv - Microbiology 2023Quote: ... nuclei were stained with DAPI (1:20,000, 10min, RT; Sigma Aldrich) or SiR-DNA dye (1 µM ...
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bioRxiv - Cell Biology 2020Quote: All usual chemicals and primers were from Sigma (St. Louis, MO, USA). Reagents for cell culture ...
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bioRxiv - Genetics 2021Quote: ... 0.2 µM each primer and 0.05 units/µL JumpStart Taq Polymerase (Sigma)) ...
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bioRxiv - Immunology 2022Quote: ... 0.5 μM of both forward and reverse primers (Table 1, Sigma Aldrich), and nuclease free water to a final volume of 50 μL ...
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bioRxiv - Plant Biology 2020Quote: ... primers (10 nmol each) and sterile Milli-Q water (Millipore India, Bangalore) to make up the volume upto 20 μl ...
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The conserved metalloprotease invadolysin is present in invertebrate haemolymph and vertebrate bloodbioRxiv - Cell Biology 2019Quote: ... Primers designed for the site-directed mutagenesis reaction were obtained from Sigma and were HPLC-purified ...
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bioRxiv - Cell Biology 2021Quote: ... Pdk1 (NM_172665) and Pdk2 (NM_133667) (all KiCqStart™ primers KSPQ12012, Sigma Aldrich) were carried out using the SYBRGreen Master mix (Thermo Fisher Scientific ...
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bioRxiv - Microbiology 2020Quote: ... and desalted or PAGE-purified oligonucleotide primers (Sigma-Aldrich, St Louis, MO) was performed according to manufacturers’ instructions ...
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bioRxiv - Physiology 2020Quote: ... and were purchased from Sigma-Aldrich (see Table 3 for primer details). All reactions were performed in duplicate on 384-well MicroAmp optical plates (Applied Biosystems ...
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bioRxiv - Microbiology 2020Quote: ... Primers used in this paper (Table S3) were procured from Sigma-Aldrich. PCR products were checked by gel electrophoresis on 1 % (w/v ...
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bioRxiv - Developmental Biology 2022Quote: ... was 10 ul consisting of 0.4 of ul forward primer (Sigma Aldrich), 0.4 ul of reverse primer (Sigma Aldrich) ...