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Citations for Millipore Sigma :
8701 - 8750 of 10000+ citations for Recombinant Human MSLN Protein Fc tagged since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2022Quote: ... The refolded protein was concentrated with Amicon Ultra-15 Centrifugal Filter Units (100 kDa NMWL, Merck Millipore) and remaining urea removed with a PD10 desalting column (GE Healthcare) ...
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bioRxiv - Molecular Biology 2022Quote: ... Bound proteins were eluted using IP buffer containing 0.25 mg/mL of 3x FLAG peptides (Sigma-Aldrich) at 25 °C for 5 min ...
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bioRxiv - Plant Biology 2022Quote: ... proteins and DNA fragments were then immuno-precipitated using antibodies against anti-FLAG (SIGMA, catalog no. M2), H3Ac (Millipore ...
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bioRxiv - Microbiology 2022Quote: Total protein was determined by Coomassie blue method using Bradford dye (Sigma-Aldrich, St. Louis, MO, USA) and absorbance was measured at 595 nm ...
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bioRxiv - Genetics 2022Quote: ... Protein samples were prepared in 4X NuPAGE LDS Sample Buffer with 0.5% b-mercaptoethanol (Sigma, catalog# M6250) for all proteins except for PC1 and PC2 and their loading control beta-actin ...
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bioRxiv - Biochemistry 2022Quote: ... 150mM NaCl and bound protein was eluted with the same buffer containing 2.5 mM desthiobiotin (Sigma-Aldrich). Proteins were spun at 100,000x g to remove aggregates before use in assays ...
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bioRxiv - Developmental Biology 2022Quote: ... Proteins were eluted by the addition of elution buffer containing 3 x Flag peptide (Sigma, cat. #F4799) at 4°C for 30 min ...
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bioRxiv - Biochemistry 2022Quote: ... The protein was concentrated to 8.0 mg/ml using 100k molecular weight cutoff Amicon columns (UFC510024, Millipore) and further purified by gel filtration chromatography (Superose 6 increase 3.2/300 GL ...
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bioRxiv - Biochemistry 2022Quote: ... Clarified lysate was filtered through a 0.2 µm low-protein binding syringe filter (Millex Durapore; EMD Millipore) and applied to glutathione Sepharose 4b resin (Cytiva ...
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bioRxiv - Cell Biology 2022Quote: ... The proteins were then transferred to a Amersham™ Protran® Western-Blotting-Membrane (#GE10600044, Sigma-Aldrich) at 120 V for 1 h ...
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bioRxiv - Biochemistry 2021Quote: Denatured proteins were separated by SDS-PAGE and transferred to Immobilon-FL PVDF membranes (Merck Millipore IPFL00010). Membranes were incubated in a casein-based blocking buffer (Sigma-Aldrich ...
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bioRxiv - Microbiology 2020Quote: ... 2 µl of the supernatant were used for protein quantification using the Direct Detect system (Merck Millipore).
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bioRxiv - Microbiology 2021Quote: ... GGG-azide labelled proteins were concentrated to 25 μM on a 30 kDa Amicon Tube (Merck Millipore) in Tris/NaCl buffer and next labelled with 100 μM DBCO-Cy5 (Sigma Aldrich ...
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bioRxiv - Cell Biology 2021Quote: ... The protein samples were separated by SDS-PAGE and transferred to PVDF membranes (Merck Millipore, Burlington, MA) by electroblotting ...
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bioRxiv - Microbiology 2020Quote: ... The proteins were detected using the primary antibody (Mouse α-His at 1:2500 dilution, Sigma Aldrich) followed by the HRP conjugated secondary antibody (Sheep α-Mouse at 1:7000 dilution ...
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bioRxiv - Molecular Biology 2020Quote: ... Protein extracts were prepared and TRF2-containing complexes were immunoprecipitated using anti-FLAG M2 affinity gel (Sigma). Following the IP ...
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bioRxiv - Biophysics 2020Quote: The RBD and spike proteins were chemically biotinylated using the EZ-Link NHS-PEG4-Biotin kit (Sigma). For the RBD ...
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bioRxiv - Neuroscience 2020Quote: ... The protein solution was reconstituted into mesophase using molten monoolein spiked with 5% (w/w) cholesterol (Sigma) in 2:3 ratio of protein solution ...
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bioRxiv - Microbiology 2021Quote: ... Equivalent protein amounts were resolved by SDS-PAGE and subsequently transferred to Immobilon PVDF-FL membranes (Millipore). Blots were probed using mouse anti-Flag M2 (1:3000 ...
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bioRxiv - Bioengineering 2019Quote: RanBP9 was immunoprecipitated from total protein extracts using monoclonal αHA-Agarose antibody (Sigma cat. nr. A2095-1ML). Briefly ...
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bioRxiv - Microbiology 2021Quote: ... The purified protein was further concentrated on Amicon Ultra Centrifugal Filters with 50 KDa cutoff (Sigma-Aldrich).
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bioRxiv - Neuroscience 2021Quote: ... After that sections were incubated with rabbit polyclonal anti-glial fibrillary acidic protein (GFAP, Cat. No.G9269, Sigma–Aldrich Co. ...
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bioRxiv - Biochemistry 2021Quote: ... Elution fractions containing the protein were pooled and concentrated (Amicon Ultra 10 kDa cut off, Millipore Sigma) for gel filtration ...
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bioRxiv - Microbiology 2020Quote: IgG from rabbit sera was affinity-purified with equal parts of Protein G Sepharose (Sigma-Aldrich, P3296) and 150 ml Protein A Sepharose (GE ...
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bioRxiv - Microbiology 2020Quote: ... Protein was extracted by resuspending the pellets in 100 mM triethylammonium bicarbonate (TEAB; Sigma-Aldrich, MO, USA) 4% sodium dodecyl sulfate (SDS ...
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bioRxiv - Physiology 2021Quote: Total protein was extracted from snap frozen tissue and cells using RIPA buffer (Sigma-Aldrich, Dorset, UK), with protease (1/100 ...
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bioRxiv - Neuroscience 2020Quote: ... proteins (20 μg per lane) were separated by SDS-PAGE and transferred to a PVDF membrane (Millipore). Membranes were blocked in 4% milk in TBST (0.05M Tris pH7.2 ...
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bioRxiv - Microbiology 2021Quote: ... Fusion FLAG-ATG5 and ATG7 proteins were captured using Anti-FLAG® M2 Magnetic Beads (Sigma-Aldrich) according to the manufacturer's instructions ...
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bioRxiv - Molecular Biology 2020Quote: Immunoprecipitations were performed on 500 μg of nuclear protein extracts using anti-flag M2 gel (Sigma-Aldrich) or by using Diagenode A/G protein-coupled magnetic beads to target endogenous proteins ...
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bioRxiv - Cancer Biology 2021Quote: ... Lysates were pre-cleared on rotator using Protein A + G mix (Millipore, 16-156 and 16-266) for 1 h at 4°C ...
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bioRxiv - Biochemistry 2021Quote: ... Proteins were extracted either in 100 μl 3 M guanidinium hy-drochloride (Gnd-HCl, Sigma-Aldrich, G3272) in 0.1 M Tris ...
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bioRxiv - Cancer Biology 2020Quote: RIP assay was performed using Magna RIP™ RNA-Binding Protein Immunoprecipitation Kit (Millipore Sigma, Burlington, MA) according to manufacturer’s instructions ...
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bioRxiv - Developmental Biology 2022Quote: ... urea was removed by washing the protein suspension three times with 50mM ammonium bicarbonate (Sigma-Aldrich, Spain). Proteins were digested first by Lys- C (Promega ...
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bioRxiv - Molecular Biology 2022Quote: ... proteins were separated from each sample by centrifugation through Amicon ® Ultra Centrifugal Filters (Merck, Millipore Ltd.) at 21.000 x g for 10 min at 4°C ...
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bioRxiv - Immunology 2022Quote: ... All lentiviral vectors (pLKO.1) expressing shRNAs used for knockdown of host proteins were purchased from Sigma.
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bioRxiv - Cancer Biology 2019Quote: ... The lyophilized proteins were resuspended in 1 ml of a 2 mg/ml DTT (1,4-Dithiothreitol, Sigma) solution and incubated at 50C for 2h ...
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bioRxiv - Bioengineering 2019Quote: ... total proteins from saliva was ultrafiltrated using an amicon ultra 15 mL centrifugal filter (Millipore, Massachusetts, USA), 20 μg of saliva total protein were subjected to SDS polyacrylamide gel electrophoresis ...
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bioRxiv - Biochemistry 2020Quote: ... The purified proteins were concentrated to 30-40 mg/mL using Amicon concentrators (30-kDa cutoff; Millipore).
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bioRxiv - Cell Biology 2019Quote: ... 1 mg of extracted protein in lysis buffer was immunoprecipitated overnight with Flag-M2 affinity gel (Sigma) at 4°C ...
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bioRxiv - Molecular Biology 2020Quote: ... 3 mg total protein were mixed with 2 mL Urea Buffer (UB) (8 M urea (Sigma, U5128) in 0.1 M Tris/HCl pH 8.0 and 50mM ammonium bicarbonate) ...
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bioRxiv - Biochemistry 2019Quote: ... The eluted protein was concentrated using an Amicon-Ultra-15 centrifugal filter (MWCO 30kDa) (Merck Millipore, USA), spin down 15 min in a bench-top centrifuge (4°C ...
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bioRxiv - Plant Biology 2020Quote: ... the protein was supplemented with 100 mM D-glucose before mixing with a 80% monoolein (Sigma-Aldrich) 20% cholesterol mixture ...
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bioRxiv - Systems Biology 2021Quote: ... Yeast proteins digestion was performed on excised bands from gel gradient and digested peptides of UPS2 (Sigma) were used as external standards for absolute protein quantification (more details in Supp ...
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bioRxiv - Plant Biology 2021Quote: ... the proteins underwent binding with secondary antibodies diluted 5000-fold in PBS (anti-rabbit IgG; Sigma-Aldrich). Protein bands were visualized by staining with Clarity Western ECL Substrate (Bio-Rad ...
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bioRxiv - Cancer Biology 2021Quote: ... Equivalent amounts of protein per sample were separated by SDS-PAGE and transferred onto PVDF membranes (Millipore). Membranes were blocked with 5% non-fat milk or 5% BSA in TBST (Tris-buffered saline containing Tween-20 ...
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bioRxiv - Systems Biology 2021Quote: ... Samples were digested overnight at 37°C using trypsin (1:100 w/w, enzyme/protein, Sigma-Aldrich) and LysC (1:100 w/w ...
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bioRxiv - Physiology 2021Quote: ... Protein was detected using a secondary HRP-linked antibody and Luminata Classico Western HRP Substrate (Millipore WBLUC0020). To reprobe membranes ...
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bioRxiv - Microbiology 2019Quote: ... The extracted proteins were subjected to 12% SDS-PAGE and then transferred onto Polyvinylidene Fluoride (Sigma, Germany). The immobilized filter was blocked overnight at 4 °C in 5% skimmed milk in PBST (PBS with 0.05 % (v/v ...
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bioRxiv - Microbiology 2020Quote: ... lysates were diluted 1:1 in RIPA buffer and precleared with protein A agarose beads (Millipore-Sigma) for 60 min at 4°C ...
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bioRxiv - Microbiology 2020Quote: ... lysates were diluted 1:1 in RIPA buffer and precleared with protein A agarose beads (Millipore-Sigma) for 60 min at 4°C ...