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Citations for Millipore Sigma :
8551 - 8600 of 10000+ citations for Rat Epithelial membrane protein 1 EPN3 ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2023Quote: ... Protein concentration was then measured using a Bradford assay (#B6916, Sigma), after which the proteins were diluted in Promega buffer (50 mM NaCl ...
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bioRxiv - Immunology 2023Quote: ... injection of 30 μg of OVA protein (Grade V; Sigma-Aldrich) adsorbed to 2 mg of aluminum hydroxide (alum ...
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bioRxiv - Microbiology 2023Quote: ... Target proteins were visualized using Immobilon Western Chemiluminescent HRP substrate (Millipore).
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bioRxiv - Microbiology 2023Quote: ... Protein concentration was determined by the Bradford method (Sigma-Aldrich, B6916). 50 μL magnetic beads (Invitrogen ...
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bioRxiv - Biophysics 2023Quote: ... Purified proteins were concentrated using Amicon Ultra 3K MWCO filters (Millipore) in storage buffer (50 mM Tris-HCl pH 7.6 ...
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bioRxiv - Biophysics 2023Quote: ... Protein expression was performed in Bl21 (DE3) pLys competent cells (Novagen) using an L.B ...
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bioRxiv - Biochemistry 2023Quote: ... 25 µg of protein was reduced with 2 mM dithiothreitol (Sigma) for 60 minutes at 55 °C ...
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bioRxiv - Biochemistry 2023Quote: ... Cells were also lysed in BugBuster Protein Extraction buffer (EMD Millipore) for 30 min instead of sonication.
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bioRxiv - Cell Biology 2023Quote: ... Cenexin rabbit antibody (Protein tech) and centrin mouse antibody (EMD Millipore) diluted 1:1000 for centrin and 1:200 for other centrosomal proteins in 1% BSA in PBS for 1.5 hours at room temperature ...
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bioRxiv - Plant Biology 2023Quote: ... The recombinant fusion protein was purified with Glutathione Sepharose beads (Sigma). Definite probes were designed for different cis-elements and their mutated versions (Table S3) ...
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bioRxiv - Physiology 2024Quote: Proteins were isolated from the liver using CelLyticTM MT (Sigma, C3228) in the presence of a protease inhibitor/phosphatase inhibitor cocktail (Thermo Fisher Scientific ...
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bioRxiv - Neuroscience 2024Quote: ... the proteins were transferred to Immobilon-P PVSF (0.45µm, Sigma-Millipore) in Tris-Glycine buffer supplemented with 20% methanol for 1 h at 75V ...
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bioRxiv - Neuroscience 2024Quote: ... the proteins were transferred to Immobilon-P PVSF (0.45µm, Sigma-Millipore) in Tris-Glycine buffer supplemented with 20% methanol for 1 h at 75V ...
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bioRxiv - Biochemistry 2024Quote: ... Protein expression was induced with isopropyl-b-D-thiogalactopyranoside (IPTG, Millipore) at 100 uM and cells were grown overnight at 16°C ...
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bioRxiv - Bioengineering 2024Quote: ... Protein concentration was calculated following Bradford assay (Bradford reagent, Sigma-Aldrich). A 1:50 mixture of β-mercaptoethanol (Acros Organics ...
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bioRxiv - Neuroscience 2024Quote: ... Protein fragments were expressed in Escherichia coli BL21 Rosetta (DE3) (Novagen) cells ...
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bioRxiv - Microbiology 2024Quote: ... The protein was concentrated on Amicon Ultracel 10K centrifugal filters (Millipore) to 23 mg/ml concentration.
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bioRxiv - Cell Biology 2024Quote: ... Protein samples were denatured with 5 % v/v 2-mercaptoethanol (Sigma) for 5 min at 95 °C before they were loaded in gradient Bolt Bis-Tris gels (Thermo) ...
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bioRxiv - Cancer Biology 2024Quote: Protein lysates were harvested with RIPA lysis buffer (Sigma Aldrich; R0278) containing protease inhibitor cocktail (Thermo Fisher Scientific ...
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bioRxiv - Biochemistry 2024Quote: ... A dilution series of the protein synthesis inhibitor cycloheximide (Sigma 01810) was created in water ...
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bioRxiv - Neuroscience 2024Quote: ... the FLAG interacting protein mix was eluted by 3xFLAG peptides (Sigma).
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bioRxiv - Cell Biology 2024Quote: ... Bound proteins were eluted with 5μg/ml FLAG peptide (Sigma, F3290) in buffer and subsequently separated on 4-12% NuPAGE Novex Bis-Tris Minigels (Invitrogen) ...
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bioRxiv - Bioengineering 2024Quote: ... we diluted fluorescently tagged BSA protein (Sigma-Aldrich, St. Louis, MO) to a concentration of 0.1 mg/mL and encapsulated the solution in 45-μm droplets using the single-emulsion droplet-generation chip ...
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bioRxiv - Cell Biology 2024Quote: ... Equal protein loading was confirmed using Ponceau S Staining (P7170, Sigma). Blots were stained using indicated antibodies (Supplementary Table 2) ...
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bioRxiv - Bioengineering 2024Quote: Proteins were extracted from fibroblast cells using RIPA Buffer (Sigma #R0278) with protease inhibitors (Roche #11836153001) ...
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bioRxiv - Molecular Biology 2024Quote: ... and Etanercept chimeric proteins (Millipore Sigma Cat No. 185243-69-0), representing the extracellular domain of the target receptor fused to the Fc region of immunoglobulin G ...
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bioRxiv - Molecular Biology 2024Quote: ... The concentrated protein underwent further processing with centrifugal filter units (Millipore). The purified SsCBE2-C2 protein was then stored at -80°C.
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bioRxiv - Biochemistry 2024Quote: Fusion proteins were expressed in BL21(DE3)pLysS E.coli (EMD Millipore). The proteins were tagged at the N-terminus with his6-HT7 (pET30-HT7 vector) ...
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bioRxiv - Genomics 2024Quote: ... and proteins were stained with Coomassie Brilliant Blue G-250 (Sigma).
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bioRxiv - Physiology 2024Quote: ... The protein concentration was measured using a Bradford Reagent (#B6916; Sigma). Protein samples (40μg ...
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bioRxiv - Developmental Biology 2024Quote: ... The PROX1-FLAG protein was then purified on M2 agarose (Sigma), which captures FLAG-tagged proteins as described previously 52 ...
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bioRxiv - Evolutionary Biology 2024Quote: All recombinant proteins were expressed using pET-11a (Sigma #69436-3) based vectors in BL21(DE3 ...
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bioRxiv - Cell Biology 2024Quote: Proteins were extracted from cell pellets resuspended in RIPA buffer (Sigma) containing cOmpleteTM Mini Protease Inhibitor Cocktail (Merck ...
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bioRxiv - Cancer Biology 2021Quote: ... Membranes were probed overnight at 4°C with mouse anti-BRAF [clone 3C6] (Sigma-Aldrich, cat# WH0000673M1; RRID:AB_1840330) at 2.5 µg/ml dilution ...
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bioRxiv - Cell Biology 2020Quote: Eluted samples were resolved on standard sodium dodecyl sulphate-polyacrylamide gels and transferred to polyvinylidene difluoride membrane (Millipore). Membranes were blocked in 3% BSA in 0.2% Tween-20 in PBS for 1 hour ...
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bioRxiv - Immunology 2021Quote: ... Membranes were washed twice for 5 min in PBS plus 0.1% (v/v) Triton X-100 (Sigma-Aldrich) before being blocked with a 1% (w/v ...
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bioRxiv - Developmental Biology 2021Quote: ... embryos with dissociated blastomeres enclosed in the fertilization membrane were incubated with 100 µg/ml cycloheximide (Sigma, C7698) in CMFM ...
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bioRxiv - Microbiology 2021Quote: ... through 0.22 µm pore size Millipore hydrophilic polyethersulfone membranes of 47 mm diameter (Sigma-Aldrich, St. Louis, USA). Filters were stored at -80 °C until DNA extraction.
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bioRxiv - Neuroscience 2022Quote: ... Tissue was cut into 400 μM thick sections on a tissue chopper and cultured on membranes (Millipore PICMORG50) at 37 °C in 5% CO2 ...
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bioRxiv - Neuroscience 2021Quote: ... 2 dishes per pup (split randomly between control/ test conditions)) onto sterile 0.4 μm pore membranes (Millipore #PICM0RG50) in 35 mm culture dishes (Nunc) ...
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bioRxiv - Molecular Biology 2021Quote: ... Samples were separated on SDS-PAGE gels and transferred to Immobilon-P transfer membranes (cat# IPVH00010, Merck Millipore). The following antibodies were used to probe the membranes ...
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bioRxiv - Molecular Biology 2020Quote: ... membranes were incubated overnight at 4°C with one of the following primary antibodies: FLAG (Sigma-Aldrich, F3165), γH2AX (Millipore ...
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bioRxiv - Molecular Biology 2021Quote: Membrane potential (ΔΨ) assessment was carried out with the fluorescent potentiometric dye tetramethylrhodamine methyl ester (TMRM; Sigma-Aldrich) working in quench mode (1 µM) ...
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bioRxiv - Cell Biology 2022Quote: Migration assays were performed in transwell polycarbonate membrane cell culture inserts with a 5µm pore (Corning, Sigma-Aldrich) using 1.25×105 hTERT and primary MSCs ...
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bioRxiv - Immunology 2021Quote: ... cell lysates were resolved on an SDS-PAGE gel and transblotted onto a PVDF membrane (Millipore, Bedford, MA). The blots were probed with a STAT3-specific antibody (Cell Signaling Technology ...
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The evolution of red colour vision is linked to coordinated rhodopsin tuning in lycaenid butterfliesbioRxiv - Evolutionary Biology 2020Quote: ... The eluate was concentrated using an Amicon Ultra-2 Centrifugal Filter Unit with Ultracel-10 membrane (Millipore, USA), for 35 min at 4°C and 3,500rpm ...
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bioRxiv - Pathology 2020Quote: ... Protein extracts (20-30 μg) were resolved by SDS-PAGE (6.0 or 7.5 % wt/vol) and transferred electrophoretically to PVDF membranes (Millipore). After blocking with non-dry fat milk (5 % wt/vol) ...
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bioRxiv - Bioengineering 2019Quote: ... Chips fabricated with polyester terephthalate (PET) track-etched membranes were assembled using (3-Glycidyloxypropyl) trimethoxysilane (GPTMS; Sigma, 440167) and (3-Aminopropyl ...
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bioRxiv - Microbiology 2019Quote: ... and forced through a 25-mm diameter Whatman Nuclepore Track-Etched Membrane with 8-µm holes (Sigma-Aldrich). The cellular debris ...
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bioRxiv - Immunology 2020Quote: ... Membranes were blocked and probed with one of the following antibodies: a rabbit antibody to COPA (Sigma, HPA028024) or a mouse antibody to GAPDH (Santa Cruz Biotechnology ...