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Citations for Millipore Sigma :
8401 - 8450 of 10000+ citations for Rat Epithelial membrane protein 1 EPN3 ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2020Quote: ... The protein was concentrated in a 10 kDa Amicon filter (Millipore), and purified on a CHT Ceramic Hydroxyapatite Type II (Biorad ...
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bioRxiv - Bioengineering 2019Quote: ... Protein concentration was measured by the Direct Detect Spectrometer (Merck Millipore).
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bioRxiv - Neuroscience 2019Quote: ... were conjugated with 25uL MagnaChIP protein A/G beads (EMD Millipore). Freshly harvested nuclear pellets from at least 10^6 N2a cells were sheared via Dounce homogenization (15-20 strokes ...
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bioRxiv - Cell Biology 2019Quote: ... 80 μl of Magna ChIP protein A+G Magnetic Beads (Millipore) were added to each sample and incubated overnight in a rotating platform at 4 °C ...
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bioRxiv - Plant Biology 2019Quote: ... His-tagged recombinant protein was eluded with 250 mM imidazole (Sigma).
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bioRxiv - Cancer Biology 2019Quote: ... proteins were detected using either Luminata Crescendo Western HRP Substrate (Millipore) or ECL Western Blotting Analysis System (GE Healthcare ...
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bioRxiv - Cell Biology 2020Quote: ... Immunoprecipitation: 50 μL of Protein A beads suspension (Millipore 16-157) were resuspended in PBS containing 2% BSA and 5 μg of non-immune rabbit polyclonal immunoglobulins (ab37415-5 ...
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bioRxiv - Systems Biology 2020Quote: ... Samples were pre-cleared with PureProteome Protein G magnetic beads (Millipore) at 4°C and 5% of each sample was aliquoted as a percent input control ...
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bioRxiv - Biochemistry 2020Quote: ... The calibration mix (Protein Standard Mix 15 – 600 kDa, Merck Millipore) was composed of the following proteins ...
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bioRxiv - Genetics 2019Quote: ... Bound proteins were eluted using a 3XFLAG peptide (Sigma-Aldrich, F4799) as described in [34] ...
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CHD7 interacts with the nucleosome acidic patch for its efficient activity via its N-terminal regionbioRxiv - Biochemistry 2020Quote: ... The protein was concentrated using Amicon Ultra centrifugal filter devices (Millipore). CHD7ΔN-CRD and CHD7N-CRD_RA were also purified as the wild type full length CHD7.
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bioRxiv - Biochemistry 2020Quote: ... Proteins were co-expressed in Rosetta 2(DE3)pLysS Ecoli (Novagen). Cells were grown at 37°C in 2XYT media to an OD600 of 0.8 ...
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bioRxiv - Immunology 2020Quote: ... Proteins were visualized using Immobilon Forte Western HRP Substrate (Millipore, WBLUF0500).
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bioRxiv - Molecular Biology 2020Quote: ... followed by silver-staining (for proteins, silver nitrate purchased from Sigma) and Western blotting (specific proteins of interest) ...
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bioRxiv - Microbiology 2020Quote: ... Proteins were eluted using 200 μg/mL 3xFlag peptide (F4799, Sigma) in lysis buffer by incubation on an orbital shaker (1400 rpm ...
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bioRxiv - Plant Biology 2021Quote: ... Fusion proteins were purified using Ni2+-containing His-Bind columns (Novagen). Purified proteins were desalted and concentrated by ultracentrifugation (Amicon ...
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bioRxiv - Pathology 2021Quote: ... The immune complexes were incubated with protein A-Sepharose beads (Sigma) at 4 °C for 1 h ...
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bioRxiv - Pathology 2021Quote: ... The immune complexes were incubated with protein A-Sepharose beads (Sigma) at 4 °C for 1 h ...
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bioRxiv - Immunology 2020Quote: A commercial protein-A affinity chromatography called PURE-1A (Sigma-Aldrich.) was used to purify mammalian immunoglobulins from the serum of different mammalian species including horse ...
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bioRxiv - Cell Biology 2019Quote: ... Protein concentration for lysates was determined using Bradford reagent (Sigma-Aldrich), loads were adjusted and proteins were resolved by SDS-PAGE and analyzed by western-blot (Western Lightning Plus-ECL kit ...
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The evolution of red colour vision is linked to coordinated rhodopsin tuning in lycaenid butterfliesbioRxiv - Evolutionary Biology 2020Quote: ... The crude protein lysate concentration was quantified by BSA (Sigma-Aldrich) and 25 μg crude extract was loaded on NuPAGE™ 3-8% Tris-Acetate gels (ThermoFisher ...
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bioRxiv - Neuroscience 2019Quote: ... was incubated with recombinant His-tagged human HDAC6 protein (EMD Millipore) in 250 μl RB100 buffer (25 mM HEPES pH 7.5 ...
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bioRxiv - Genetics 2020Quote: ... Protein extraction and Western blot using anti-puromycin antibody (Merck Millipore) was performed as described before.
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bioRxiv - Genetics 2020Quote: ... or residues near the carboxylterminal end of the protein (ABN410, Millipore) with IR-700 conjugated goat anti-rabbit secondary antibody (Rockland 611130122 ...
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bioRxiv - Cell Biology 2020Quote: ... Proteins were first reduced by incubation with dithiothreitol (5mM; Sigma-Aldrich) for 30 min at 60°C ...
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bioRxiv - Cell Biology 2021Quote: ... protein was purified by incubating with glutathione agarose (GST; Sigma Aldrich) or amylose resin (MBP ...
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bioRxiv - Physiology 2020Quote: ... as a carrier protein using 5% glutaraldehyde (Sigma-Aldrich, Gillingham, UK)in phosphate buffer (0.1 M ...
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bioRxiv - Molecular Biology 2021Quote: ... immunoprecipitated proteins were eluted using 3X-FLAG peptide (Sigma - catalog # F4799) for 2 hours at 4°C according to the manufacturer’s instructions.
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bioRxiv - Physiology 2020Quote: ... The protein concentration was determined by the Bradford reagent (Sigma-Aldrich). For each experiment ...
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bioRxiv - Cell Biology 2021Quote: Recombinant protein expression vectors were constructed from the pET28a series (Novagen) encoding an N-terminal hexa-histidine tag and thrombin cleavage site.
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bioRxiv - Neuroscience 2020Quote: ... Concentration of solubilized proteins was quantified using BCA assay (Sigma-aldrich). All samples were then set at the same volume and concentration for the rest of the protocol ...
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bioRxiv - Cell Biology 2019Quote: ... Proteins were harvested using ice cold RIPA buffer (Millipore 20-188) supplemented with phosphatase (Sigma 4906845001 ...
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bioRxiv - Neuroscience 2020Quote: ... Proteins were transferred onto a PVDF Immobilon-P (0.45 µm Millipore), and SMN ...
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bioRxiv - Biochemistry 2021Quote: ... FLAG-tagged proteins were purified using FLAG M2 affinity gel (Sigma) rotated end over end for 3 h at 4°C ...
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bioRxiv - Biochemistry 2021Quote: ... and bound proteins were eluted with 3.5 mM d-desthiobiotin (Sigma) in the equilibration buffer (Besche and Goldberg ...
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bioRxiv - Biochemistry 2021Quote: ... The lysates were pre-incubated with Protein- A Sepharose beads (Novagen) and allowed to mix for 30 min at 4°C ...
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bioRxiv - Biochemistry 2021Quote: ... The eluted proteins were concentrated using 30 kDa spin filters (Millipore) and further purified by size exclusion chromatography on a Superdex 200 Increase 10/300 column (GE Healthcare ...
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bioRxiv - Animal Behavior and Cognition 2021Quote: ... Protein concentration in homogenates was determined using the BCA assay (Sigma).
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bioRxiv - Biochemistry 2021Quote: ... The protein was concentrated in a 50,000 MWCO Amicon Concentrator (Millipore) to <1 mL and injected onto a Superdex™ 200 10/300 GL Increase size-exclusion column (GE Healthcare ...
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bioRxiv - Immunology 2021Quote: ... or 10 μM cytosolic protein synthesis inhibitor cycloheximide (CHX, Sigma-Aldrich) to induce mitochondrial fragmentation and hypertubulation ...
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bioRxiv - Neuroscience 2020Quote: ... proteins were visualized with chemiluminescence using ECL Lumina Forte (Millipore, France) and chemiluminescence detector (Bio-Rad ...
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bioRxiv - Neuroscience 2021Quote: ... mouse monoclonal anti-glial fibrillary acidic protein (GFAP, #G3893, Sigma-Aldrich).
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bioRxiv - Plant Biology 2022Quote: ... proteins were detected with alkaline phosphatase-conjugated antibody (Sigma-Aldrich A3687). Primary antibodies used were ...
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bioRxiv - Neuroscience 2021Quote: ... Protein bands were visualized using Immobilon Forte Western HRP Substrate (Millipore) and ChemiDoc system (BioRad).
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bioRxiv - Evolutionary Biology 2021Quote: ... Protein concentration of the supernatant was determined by Bradford Reagent (Sigma). The proteins were then separated on a 12% SDS-PAGE (40 μg protein/lane) ...
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bioRxiv - Cell Biology 2020Quote: ... The protein was eluted by digestion with Factor Xa protease (Sigma). Factor Xa protease was removed using p-Aminobenzamidine-agarose (Sigma ...
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bioRxiv - Cell Biology 2021Quote: ... and mouse GAPDH monoclonal antibody for protein loading controls (Sigma, G8795). Anti-mouse IRDye680RD and anti-rabbit IRDye800CW (Licor ...
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bioRxiv - Immunology 2020Quote: ... Cycloheximide (inhibitor of protein synthesis) was used at 10µg/mL (Sigma) and added 30 min prior to cytokine treatment of neutrophils.
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bioRxiv - Microbiology 2020Quote: ... We purified the protein using anti-FLAG M2 Affinity Gel (Sigma) according to manufacturer’s protocol and removed the FLAG tag and His6-tag with TEV digestion followed by reverse nickel affinity purification and sizeexclusion chromatography on a Superose 6 Increase column (GE Healthcare Life Sciences) ...
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bioRxiv - Microbiology 2021Quote: ... Protein extraction was performed in the presence of 2% SDS (Sigma) and 4 M acetic acid (Fisher ...