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Citations for Millipore Sigma :
8801 - 8850 of 10000+ citations for Rat Epithelial membrane protein 1 EPN3 ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2021Quote: ... g) were pre-cleared with protein A-immobilized PureProteome magnetic beads (Millipore) and incubated overnight (4°C ...
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Molecular basis of selective cytokine signaling inhibition by antibodies targeting a shared receptorbioRxiv - Immunology 2021Quote: ... 2000 response units (RU) of Protein A from Staphylococcus aureus (Sigma Aldrich) were immobilized on all channels of a CM5 sensor chip ...
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Molecular basis of selective cytokine signaling inhibition by antibodies targeting a shared receptorbioRxiv - Immunology 2021Quote: ... 2000 response units (RU) of Protein A from Staphylococcus aureus (Sigma Aldrich) were immobilized on all channels of a CM5 sensor chip ...
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bioRxiv - Neuroscience 2020Quote: ... before the protein expression was revealed by chemiluminescence using Immobilon Western (Millipore) and the signal detected using a Chemidoc MP system (BioRad) ...
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bioRxiv - Biochemistry 2021Quote: ... All remaining protein constructs were cloned into the pET28b vector (Merck Millipore). Unless otherwise stated ...
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bioRxiv - Cancer Biology 2021Quote: Proteins were extracted from cell culture by RIPA lysis buffer (Sigma Aldrich) with protease and phosphatase inhibitor cocktail and sample reducing agent (Thermo Fisher Scientific) ...
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bioRxiv - Biochemistry 2020Quote: ... Protein-antibody binding was visualized using Immobilon Forte Western HRP Substrate (Millipore) on the LAS-3000 imaging system (FUJIFILM ...
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bioRxiv - Bioengineering 2021Quote: ... Supernatant containing soluble recombinant protein was loaded into α-lactose agarose (Sigma) affinity column preequilibrated with PBS ...
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bioRxiv - Bioengineering 2021Quote: ... Proteins were eluted with a linear gradient of β-lactose (Sigma-Aldrich) in phosphate buffer.
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bioRxiv - Neuroscience 2021Quote: ... Protein was extracted from transfected meningeal cells using RIPA buffer (R0278, Sigma) and run on Western blot at 20ug per well ...
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bioRxiv - Bioengineering 2020Quote: ... total protein was collected from cell lysate in RIPA buffer (Sigma, R0278) containing 1% phosphatase inhibitor cocktail 3 (Sigma ...
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bioRxiv - Biochemistry 2021Quote: ... Proteins were visualized by using enhanced chemiluminescence (Millipore Corporation, Billerica, MA, USA). Images were acquired using Uvitec Image System (Cleaver Scientific Limited ...
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bioRxiv - Biochemistry 2020Quote: ... The partially purified protein was concentrated (10 kDa cut-off, EMD Millipore) and exchanged into 100 mM potassium phosphate buffer (pH 8.0 ...
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bioRxiv - Biochemistry 2020Quote: ... The protein was loaded onto a 0.45 μm nitrocellulose filter (HA Millipore), and the filters were washed with 3 ml 30 mM imidazole (pH 7.1) ...
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bioRxiv - Biophysics 2021Quote: Routine chemicals used for protein expression and purifications were purchased from Sigma, Fisher Scientific ...
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bioRxiv - Neuroscience 2021Quote: ... Supernatant was recovered and proteins were quantified with Bradford assay (Sigma Aldrich) and samples were stored at -80°C until further used ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... Transferred protein quantities were detected with Ponseau S solution (Sigma-Aldrich, USA). Transferred proteins were blocked with 1% BSA (KPL Detector™ Block ...
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bioRxiv - Cell Biology 2021Quote: ... and protein was eluted with 100 µg/mL FLAG peptide (Sigma-Aldrich) in elution buffer for 1 hour at 4°C with rotation ...
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bioRxiv - Biochemistry 2021Quote: ... protein expression and purification and enzyme assays were obtained from Sigma-Aldrich, TCI chemicals ...
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bioRxiv - Biochemistry 2021Quote: ... the protein samples were reduced with 20 mmol/L dithiothreitol (DTT, Sigma) at 37°C for 1 h and alkylated with 50 mmol/L iodoacetamide (IAA ...
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bioRxiv - Biochemistry 2020Quote: ... Purified protein was incubated on ice with dried cardiolipin (Sigma-Aldrich, USA) to a final lipid concentration of 0.1 mg/mL ...
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bioRxiv - Biochemistry 2020Quote: Protein Kinase A Catalytic Subunit from bovine heart (PKA, EC 2.7.11.11, Sigma) was reconstituted in bi-distilled water containing 6 mg/ml DTT at a concentration of 50 μg/ml ...
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bioRxiv - Biochemistry 2020Quote: ... Solubilized protein was then loaded onto 2 mL M2 affinity matrix (Sigma) previously equilibrated with wash buffer (50 mM Tris-HCl pH 7.4 ...
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bioRxiv - Cell Biology 2021Quote: ... Protein extracts were preparared using RIPA lysis buffer (Sigma, ST. Louis, USA) supplemented with protease inhibitor cocktail (Complete ...
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bioRxiv - Cell Biology 2021Quote: ... The concentration of the isolated proteins was determined using Bradford reagent (Sigma). 30-50 micrograms of the protein were separated on a 15% polyacrylamide gel and electrophoretically transferred to PVDF membranes (Millipore) ...
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bioRxiv - Systems Biology 2020Quote: ... Protein G sepharose was used for anti-SOCS1 (Millipore, 04-002, RRID:AB_612104) IP ...
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bioRxiv - Neuroscience 2021Quote: Proteins from spinal cord tissue were extracted with RIPA buffer (Sigma Aldrich) containing a cocktail of protease inhibitors (Roche) ...
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bioRxiv - Microbiology 2021Quote: ... Protein content of the cell lysates was determined using BSA assay (Sigma).
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bioRxiv - Plant Biology 2021Quote: Proteins were purified using HisSelect Sepharose beads (Sigma-Aldrich, St. Louis, USA) and the in vitro kinase reactions were carried out as described earlier (Huesmann et al. ...
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bioRxiv - Plant Biology 2020Quote: OsDMI3-GST fusion protein was produced in Escherichia coli Rosetta (DE3; Novagen), and tested for interaction as described previously (Ni et al. ...
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bioRxiv - Synthetic Biology 2021Quote: ... The target proteins were collected and desalted using Amicon (10kDa cut) (Millipore) before subjecting to the HiTrap Q column (GE Healthcare) ...
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bioRxiv - Microbiology 2021Quote: ... FLAG-tagged proteins were eluted by competition using the FLAG peptide (Sigma).
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bioRxiv - Microbiology 2020Quote: ... Target proteins were visualized with Immobilon Western Chemiluminescent HRP Substrate (Merck Millipore).
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bioRxiv - Microbiology 2020Quote: ... GST bound protein was then cleaved using biotinylated thrombin (Merck Millipore; 69672) according to the manufacturers instructions overnight at 4°C ...
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bioRxiv - Cell Biology 2021Quote: ... Total protein extracts supplemented with β-mercaptoethanol (750 mM final, Sigma-Aldrich) were analysed by western blot ...
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bioRxiv - Cell Biology 2020Quote: ... Then pre-processed protein A agarose (Catalog No. P3476, Sigma, MO, USA) was added to mixture ...
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bioRxiv - Biophysics 2021Quote: ... Protein expression was performed in Origami™ 2 (DE3) competent cells (Novagen) using LB medium ...
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bioRxiv - Biophysics 2021Quote: ... Crosslinked proteins were precipitated with 20% (w/v) trichloroacetic acid (TCA, Sigma) on ice for 90 minutes ...
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bioRxiv - Biophysics 2020Quote: ... Proteins were visualized by gel staining with Coomassie Brilliant Blue (Sigma Aldrich).
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bioRxiv - Cancer Biology 2021Quote: ... Antibody-reactive proteins were detected by western chemiluminescent HRP substrate (ECL) (Millipore). Images were acquired by FluorChem FC3 image analyzer (Molecular Devices).
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bioRxiv - Biochemistry 2022Quote: ... The protein was filtered with a 0.22 μm pore size filter (Millipore) prior to size-exclusion chromatography (SEC ...
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bioRxiv - Microbiology 2022Quote: ... The proteins were detected with Luminata Forte Western HRP Substrate (EMD Millipore) or SuperSignal™ West Atto Ultimate Sensitivity Substrate (A38555 ...
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bioRxiv - Molecular Biology 2022Quote: ... and it was determined by BCA protein assay (Sigma, Sao Paulo, Brazil). A total of 10 µg protein concentrate was used in a 10% or 12% polyacrylamide gel and transferred to a nitrocellulose membrane ...
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bioRxiv - Molecular Biology 2022Quote: ... The protein was concentrated with ultra-filter 0.5 (Millipore, Sao Paulo, Brazil), and it was determined by BCA protein assay (Sigma ...
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bioRxiv - Biochemistry 2022Quote: ... 50µL of a suspension of Protein G coupled to agarose beads (Sigma) previously equilibrated in Bax buffer (20mM Tris/HCl pH 8.0 ...
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bioRxiv - Cell Biology 2022Quote: ... 500μg of protein was incubated on magnetic beads (FLAG M2 beads (Sigma), GFP beads (Chromotek ...
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bioRxiv - Molecular Biology 2022Quote: ... Total protein was assayed by incubation with Ponceau staining (Sigma-Aldrich P7170), which was later destained and imaged on a Sapphire biomolecular imager (Azure Biosystems) ...
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bioRxiv - Molecular Biology 2022Quote: ... proteins entrapped in gel bands were reduced with 10 mM dithiothreitol (Sigma) at 60°C for 1 hour ...
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bioRxiv - Neuroscience 2022Quote: ... The final measurements were normalized to protein content using sulforhodamine B (Sigma) (Johnson et al. ...
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bioRxiv - Bioengineering 2022Quote: Biotinylated proteins were obtained using Biotin N-hydroxysuccinimide ester reagent (Sigma Aldrich) according to the manufacturer’s protocol.