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Citations for Millipore Sigma :
8651 - 8700 of 10000+ citations for Rat Epithelial membrane protein 1 EPN3 ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2021Quote: ... the liposome suspension was extruded through a polycarbonate membrane filter with a 200-nm pore size (Merck Millipore). Smaller liposomes were prepared by sonicating the liposome suspension ...
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bioRxiv - Developmental Biology 2021Quote: Nuclear extracts were separated on a 7% SDS-PAGE gel and transferred to Immobilon polyvinylidene difluoride membrane (Millipore). The membranes were blocked with Tris-buffered saline Tween [10 mM Tris (pH 8.0) ...
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bioRxiv - Cell Biology 2019Quote: ... Lysates were resolved on 7.5–10% SDS–PAGE gels and transferred to PVDF membranes (Millipore, Billerica, MA, USA). The antibody for detection of β-actin (sc-1616 ...
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bioRxiv - Biochemistry 2019Quote: ... Amicon ultra centrifugal filter units (MWCO 3500) and PVDF membrane (0.45 µm) were purchased from Millipore (Wisconsin, USA). The sequencing-grade trypsin was from Promega ...
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bioRxiv - Cell Biology 2019Quote: ... Blotted nitrocellulose membranes were incubated with primary antibodies (mouse -p53, SC-126, Santa Cruz Biotechnology; rabbit -CTCF, Millipore 07-729 ...
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bioRxiv - Genetics 2020Quote: ... boiled for 5 min and loaded onto 10% SDS-PAGE gels and transferred onto a PVDF membrane (Millipore). The membranes were blocked with 5% non-fat milk for 2 hr at room temperature ...
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bioRxiv - Genetics 2020Quote: ... cells were UV-irradiated (60 J m-2) through isopore polycarbonate membranes containing 3-μm-diameter pores (Millipore). For CPD immunodetection ...
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bioRxiv - Cell Biology 2020Quote: ... Cell lysates (20-40 μg) were subjected to 8-15% SDS-PAGE and transferred to nitrocellulose membranes (Millipore). The membrane was blocked using 5% milk in TBST buffer at room temperature for 1 h ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... Membranes were then washed three times with TBS-T and incubated with alkaline phosphatase-conjugated secondary antibodies (Sigma) in 5% milk in TBS-T for 30 min at room temperature ...
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bioRxiv - Molecular Biology 2021Quote: ... The membrane was blocked with 5% milk and incubated overnight with primary antibodies: anti-Flag antibody (Sigma, F3165), anti-MGMT antibody (Proteintech ...
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bioRxiv - Cancer Biology 2021Quote: ... Cell lysates were subjected to polyacrylamide gel electrophoresis and transferred onto Immobilion polyvinylidene difluoride membranes (Millipore, Bedford, MA). These membranes were then blocked with 5% milk or 5% BSA and probed with antibodies specific to RBM3 (Abcam Cambridge ...
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bioRxiv - Microbiology 2020Quote: ... The supernatant containing the pseudotyped lentiviral particles was harvested 2 to 3 days after transfection and filtered through 0.45μm CA membranes (Millipore). For transduction ...
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bioRxiv - Neuroscience 2021Quote: ... 15 min) followed by filtering of the supernatant with a 0.45 μm Millex PVDF membrane filter (Merck Millipore). For subsequent FPLC purification (Fast Protein Liquid Chromatography ...
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bioRxiv - Pathology 2021Quote: ... The membrane was washed three more times in TBST before addition of Immobilon Western Chemiluminescence HRP Substrate (Millipore) and visualization via film development ...
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bioRxiv - Microbiology 2020Quote: ... followed by a mid-filter of 5 μm pore size (Mixed cellulose ester membrane filter; Merck Millipore, USA) and finally through a 0.22 μm collection filter (Cellulose mixed ester membrane filter ...
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bioRxiv - Microbiology 2020Quote: ... Virus-containing supernatant was collected 48-72 hours post-transfection and filtered through 0.45um PES membrane filter (Millipore). For infection ...
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bioRxiv - Microbiology 2020Quote: ... 100 μL of bacterial cell suspension was added dropwise onto an Isopore membrane polycarbonate filter (HTTP01300, Millipore, UK). The filter was trimmed beforehand with a razor blade to identify the surface with inoculum ...
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bioRxiv - Molecular Biology 2020Quote: ... The samples were separated by SDS-PAGE and electrotransferred to a 0.45 μm Immobilon-P PVDF membrane (Millipore). The membrane was incubated overnight at 4°C with DCP5 antibodies (provided by Rémy Merret and Cécile Bousquet-Antonelli ...
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bioRxiv - Immunology 2020Quote: ... The supernatant was subsequently filtered with a Centricon Plus-80 with Biomax-PB membrane (NMWL100,000) (Millipore, Bedford, MA) and stored in liquid nitrogen to be used as viral stock for each macaque made from her own PBMCs ...
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bioRxiv - Immunology 2020Quote: ... centrifuged 20 min at 3000g and filter-sterilized using a 0.22 μm membrane vacuum filter (Express PLUS, Millipore). Filtered supernatant was loaded on a 5 ml MabSelect SuRe column (GE Healthcare ...
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bioRxiv - Immunology 2021Quote: ... 10 micrograms cell lysate was subjected to SDS-PAGE and transferred onto an Immobilon polyvinylidene difluoride membrane (Millipore). Protein detection was carried out using mouse monoclonal Ab against IκBα (#4814 ...
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bioRxiv - Microbiology 2021Quote: ... Culture suspensions were passed through Sterivex™ 0.22 µm membrane filter units (EMD Millipore Corporation, Billerica, MA, US) to capture cells ...
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bioRxiv - Neuroscience 2021Quote: ... To strip membranes and re-probe with a different antibody ReBlot Plus strong antibody stripping solution (EMD Millipore) was employed according to the manufacturer’s instructions prior to re-blocking in 5% milk and staining ...
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bioRxiv - Neuroscience 2021Quote: ... Membranes were stripped by 2 x 7 min incubations in stripping buffer (6 M guanidine hydrochloride (Sigma: G3272) with 1:150 β-mercaptoethanol ...
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bioRxiv - Neuroscience 2022Quote: ... BCA Protein Assay Kit was used to measure the protein concentration following manufacturer’s instructions and the proteins were separated using (7.5-15%) polyacrylamide gel and were transferred to a nitrocellulose membrane which has a 0.2μm pores size (Millipore). The total protein levels were visualized and normalized on the membrane ...
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bioRxiv - Microbiology 2022Quote: Culture was harvested by filtering 2 ml of exponentially growing or starving bacterial culture at 0.5 OD through a 0.22-micron filter (Durapore 0.22 μM PVDF membrane, Millipore GVWP02500) held on a vacuum micro-filtration device (25mm glass filter holder ...
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bioRxiv - Neuroscience 2022Quote: ... 11 COCS were placed and cultured on a Millicel-CM Biopore PTFE membrane insert (6-well inserts, Millipore) in order to determine the PrPC expression level based on a sandwich enzyme linked immunoabsorbent assay (ELISA ...
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bioRxiv - Bioengineering 2022Quote: MSC EVs from flask and bioreactor culture were labeled separately with lipid membrane PKH67 dye (Sigma-Aldrich, PKH67GL) as previously described [23] ...
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bioRxiv - Plant Biology 2022Quote: ... Culture filtrate was harvested and filtered through a 0.45 µm HVLP filter membrane (Merk Millipore Ltd., Cork, Ireland) and dialyzed overnight against water using 3.5 kDa molecular weight cut off Snake Skin dialysis tubing (Thermo Scientific ...
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bioRxiv - Molecular Biology 2022Quote: ... and cells were collected by filtration on a 25-mm diameter membrane filter (0.45-µm pore size; Millipore). For conjugation ...
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bioRxiv - Microbiology 2022Quote: ... The membrane was subjected to Western blot analysis using a polyclonal antibody against the 6Histidine tag (Sigma-Aldrich). After incubation with the second anti-mouse antibody (Invitrogen ...
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bioRxiv - Microbiology 2022Quote: ... and 50 mL were filtered from the samples using the standardized membrane filtration method using 0.22μm diameter cellulose filter (Millipore). The membranes were placed on 2 MacConkey agar media (Oxoid ...
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bioRxiv - Neuroscience 2023Quote: ... Centrifuged plasma was filtered through 0.8 μm filter units (MF-Millipore MCE membrane, SLAA033SB; Millipore, Burlington, MA, US) and stored at −80°C until further use.
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bioRxiv - Plant Biology 2023Quote: ... The material from the melted and digested plug was then dialyzed over a 0.1 µm dialysis membrane (Millipore) floating on a TE wash buffer for 2 h ...
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bioRxiv - Synthetic Biology 2023Quote: ... PVDF membrane was blocked with 5% milk and stained with monoclonal anti-polyHistidine primary antibody (Sigma Aldrich, H1029) and polyclonal anti-mouse secondary antibody ...
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bioRxiv - Neuroscience 2022Quote: ... Sections were transferred onto sterile membrane culture inserts (Millicell-CM, Millipore; 0.4 μm pore size, 30 mm diameter). These were placed into either a glass-bottom coverslip petri dish or a six-well plate containing pre-incubated slice culture medium (1.5-2 mL per well ...
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bioRxiv - Molecular Biology 2022Quote: The PVDF membranes were blotted with the following antibodies: anti-β-actin-peroxidase (Catalog NO. A3854; Sigma-Aldrich), anti-EBNA1 mouse monoclonal antibody (Catalog NO ...
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bioRxiv - Microbiology 2022Quote: ... The supernatant was filtered with 5.0-μm and 0.45-μm PVDF pore membrane filters (Millex-HP Syringe Filter; Merck Millipore) to remove bacterial cells ...
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bioRxiv - Microbiology 2022Quote: ... Membranes were developed using 5-bromo-4-chloro-3-indolyl phosphate (BCIP)/nitro blue tetrazolium (NBT) (Sigma Aldrich, 11697471001 ...
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bioRxiv - Cell Biology 2022Quote: ... cell lysates were subjected to SDS polyacrylamide gel electrophoresis and transferred onto polyvinylidene difluoride membranes (IPVH 00010, Millipore). The membranes were immunoblotted with the indicated antibodies and developed with Super Signal West Dura Extended Duration Substrate (34076 ...
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bioRxiv - Microbiology 2023Quote: ... triplicate culture samples for each light and temperature condition were vacuum filtered onto 0.2 µm PES membranes (Millipore) mounted on Nalgene filtration cups ...
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bioRxiv - Plant Biology 2024Quote: ... The samples were separated by SDS-PAGE and transferred to a 0.45 μm Immobilon-P PVDF membrane (Millipore). The membrane was incubated 2h at 4°C with ANTI-FLAG antibodies® M2-peroxydase (Sigma-Aldrich ...
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bioRxiv - Genomics 2024Quote: ... calceolata cells were harvested by filtration on a 1.2 µm mixed-cellulose esters membrane filter (MF-Millipore, rawp04700) with a peristaltic pump (SFP-100) ...
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bioRxiv - Cell Biology 2024Quote: ... before 1.5 ml of pollen suspension was transferred onto a filter membrane and from there onto 1.8 ml PTNT medium solidified with 0.25 % phytagel (Sigma) in a 5.5 cm Petri dish essentially as previously described (Kost et al ...
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bioRxiv - Microbiology 2024Quote: ... the membrane was incubated with the secondary antibodies (goat α-mouse, horse radish peroxidase conjugated, Sigma Aldrich, A5278). Signal was visualized using the SuperSignal West Pico PLUS Chemiluminescent Substrate (ThermoFisher Scientific ...
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bioRxiv - Microbiology 2024Quote: ... and the microorganisms of the experimental group were removed with the addition of 0.5 mL TCA and filtered onto 0.2 μm GSWP membranes (polyethersulfone, Millipore) after incubation ...
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bioRxiv - Microbiology 2024Quote: ... The supernatant containing the pseudotyped lentiviral particles was harvested 2 to 3 days after transfection and filtered through 0.45μm CA membranes (Millipore). For transduction ...
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bioRxiv - Cancer Biology 2024Quote: ... Membranes were developed using enhanced chemiluminescence (Lumi Light or Lumi Light-plus, Roche/Sigma-Aldrich, St. Louis, MO) following the manufacturer’s instructions.
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bioRxiv - Cell Biology 2023Quote: ... Protein samples (5 to 25µg/lane) were separated by 12.5% – 15% SDS-PAGE and transferred onto Immobilon-P PVDF membranes (Merck Millipore). Proteins were probed in Can Get Signal Solution1 (Toyobo ...
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bioRxiv - Genomics 2024Quote: ... The elution supernatant was passed through Amicon Ultra-0.5 Centrifugal Filter with Ultracel-100 regenerated cellulose membrane (Millipore) to remove the remaining SDS ...