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Citations for Millipore Sigma :
8751 - 8800 of 10000+ citations for Rat Epithelial membrane protein 1 EPN3 ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2021Quote: ... were fluorescently labelled by the addition of 2 μl PKH26 dye (1 mM stock concentration) from the MINI26 PKH26 Red Fluorescent Cell Linker Kit (Sigma-Aldrich) and mixed by pipetting ...
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bioRxiv - Cell Biology 2022Quote: ... cells were fixed in 70% ethanol for 1 h and stained for 10 min with an ALP staining solution (BCIP/NBT alkaline phosphatase kit, Sigma-Aldrich), according to the manufacturer’s instructions.
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bioRxiv - Microbiology 2022Quote: ... Bacteria were lysed by bead-beating in the presence of buffer RA-1 (NucleoSpin RNA isolation kit, Mackery-Nagel) and β-mercaptoethanol (Sigma). RNA isolation and purification was continued according to the protocol supplied by the manufacturer (Nucleospin RNA isolation kit ...
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bioRxiv - Developmental Biology 2019Quote: ... H9 hESCs were nucleofected using the Lonza P3 Primary Cell 4D-Nucleofector X Kit and monoclonal colonies were selected for 7-10 days with 1 μg/ml of puromycin (Sigma-Aldrich). Tetracycline hydrochloride (Sigma-Aldrich ...
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bioRxiv - Microbiology 2019Quote: RNA isolation was carried out with 2 g of soil using RNA PowerSoil Total RNA Isolation Kit (Mo Bio Laboratories, Carlsbad, CA. USA) with phenol chloroform isoamyl alcohol 25:24:1 (Sigma-Aldrich) following the instructions of the manufacturer and resulting in 100 μL of RNA solution ...
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bioRxiv - Physiology 2021Quote: ... and chemokine (C-X-C motif) ligand 1 (Cxcl1) were assessed using the MILLIPLEX-MAP kit Cytokine/Chemokine Magnetic Bead Panel (Merck Millipore, USA) according to the manufacturer’s recommendations ...
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bioRxiv - Microbiology 2020Quote: ... Peroxidase-conjugated secondary antibodies (Antgene, 1: 5000) were applied accordingly followed by image development with Chemiluminescent HRP Substrate Kit (Millipore Corporation).
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bioRxiv - Biochemistry 2022Quote: NAD total levels in MT-4 cells and MT-4 cells infected by HIV-1 were assessed using the NAD/NADH Quantification Kit (Sigma-Aldrich) according to the manufacturer’s protocol ...
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bioRxiv - Microbiology 2022Quote: ... while spotting them onto a polished stainless steel MALDI target in a 1:3 ratio (0.5 μL sample/1.5 μL matrix) with addition of 0.25 μL ProteoMass Normal Mass Calibration Mix (1:10 dilution, ProteoMass™ MALDI Calibration Kit, Sigma-Aldrich) for acquisition of high resolution spectra ...
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bioRxiv - Molecular Biology 2022Quote: CTCF Chromatin immunoprecipitation (ChIP) was performed on 1 x 107 Ter119+ erythroid cells using a ChIP Assay Kit (Millipore: 17-295) according to the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2023Quote: ... and Aβ 1-42 using the Milliplex® MAP Human Amyloid Beta and Tau Multiplex Assay kit (Millipore Sigma HNABTMAG-68K). All kits were read on a MAGPIX® system (Luminex ...
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bioRxiv - Neuroscience 2023Quote: ... PLUS and MINUS PLA probes were diluted in the Duolink Antibody diluent in 1:5 ratio with blocking buffer from the PLA kit (Millipore Sigma). The coverslips were then incubated in PLA probe solution for 1 hour at 37°C in humidity chamber ...
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bioRxiv - Cell Biology 2023Quote: ... the cells were washed three times with 1× PBS and i ncubated with anti-mouse and anti-rabbit plus PLA probes (PLA kit, Sigma-Aldrich) at 37 °C for 1 h ...
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bioRxiv - Cell Biology 2023Quote: ... and subjected to PLA with an anti-Rad51 and anti-VE-Cadherin (see Supplementary Table 1) using the Duolink in situ red starter kit (mouse/rabbit) (Sigma-Aldrich) following the manufacturer’s protocol ...
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bioRxiv - Biochemistry 2021Quote: ... the proteins were eluted with 40 µl 3x FLAG peptide (Sigma-Aldrich) and collected by centrifugation for 30 min at 100xg.
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bioRxiv - Cell Biology 2020Quote: ... Proteins were concentrated by ultrafiltration using Amicon Ultra centrifugal filters (Merck Millipore) with 50 kDa cut off ...
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bioRxiv - Cell Biology 2020Quote: ... followed by the addition of 5 mg protein A beads (Sigma, P1406) in phosphate-buffered saline (PBS ...
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bioRxiv - Immunology 2021Quote: ... 20µl of DBX-washed Magna ChIP Protein W+G magnetic beads (Millipore) were added to each sample and rotated for 1hr at 4°C ...
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bioRxiv - Genomics 2020Quote: ... Protein concentration of tissue lysates was determined using bicinchoninic acid (Sigma-Aldrich). Proteins were denatured with gel loading buffer (50 mM Tris [pH 6.8] ...
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bioRxiv - Microbiology 2021Quote: ... Protein complexes were purified with α-FLAG M2 affinity gel (Sigma-Aldrich) as described for co-IP ...
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bioRxiv - Molecular Biology 2021Quote: ... Recombinant proteins were extracted and purified using the Bugbuster reagent (Novagen, Inc). To raise antibodies against TRF4 or TIMRHOM1 ...
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bioRxiv - Immunology 2020Quote: ... and 50 μl of horseradish peroxidase-Staphylococcal protein-A conjugate (Sigma-Aldrich) diluted 1:3000 was added ...
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bioRxiv - Genomics 2020Quote: ... The fusion protein was cleaved by incubation with thrombin protease (Sigma Aldrich) and then purified with Superdex 200 pg (GE Healthcare) ...
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bioRxiv - Pathology 2022Quote: ... Target proteins were detected using ECL western blot detection solution (Millipore, WBKLS0500). Primary antibodies used for immunoblotting were as follows ...
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bioRxiv - Molecular Biology 2021Quote: ... The eluted protein was then precipitated using 100% trichloroacetic acid (Sigma-Aldrich) added drop wise to 20% of the final elution followed by vortexing and incubation at 4°C overnight prior to pelleting at 21130 x g for 20 min at 4°C ...
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bioRxiv - Biophysics 2022Quote: p53 and E2C proteins were labeled with fluorescein isothiocyanate (FITC) (Sigma-Aldrich), adapting the manufacturer’s protocol to obtain sub-stochiometric labeling enough to visualize the samples by fluorescence and confocal microscopy ...
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bioRxiv - Cell Biology 2022Quote: ... Total protein bands were reversibly stained with Fast Green FCF (Sigma, F7252) and imaged on ImageQuant LAS 4000 or Amersham ImageQuant 800 (GE Healthcare Life Sciences ...
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bioRxiv - Genomics 2020Quote: Gel-shift assays were performed using recombinant Human IRF2 protein (Sigma, #SRP6338). 10 pmoles of single-stranded oligos for surface fish IRF2 binding site and corresponding mutant cavefish IRF2 site were end-labeled with P32 radioisotope using polynucleotide kinase ...
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bioRxiv - Genomics 2020Quote: ... Cells were pelleted in Eppendorf® protein LoBind tubes (Sigma Aldrich Z666505) washed twice with ice cold PBS and snap-frozen ...
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bioRxiv - Biochemistry 2019Quote: ... All protein constructs were expressed in Escherichia coli BL21 (DE3) cells (Novagen) with a Tobacco Etch Virus (TEV ...
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Trypanosoma brucei J protein 2 functionally cooperates with the cytosolic Hsp70.4 and Hsp70 proteinsbioRxiv - Molecular Biology 2019Quote: ... The protein yield was estimated using the Bradford assay (Sigma-Aldrich, USA) with BSA as the standard ...
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bioRxiv - Biophysics 2020Quote: ... polyclonal antibody against brain lipid binding protein (BLBP) (Millipore, Merck KGaA, Germany), mouse antibody against nestin (Millipore ...
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bioRxiv - Developmental Biology 2019Quote: Protein was extracted using 1X RIPA lysis buffer (Millipore Sigma, Burlington MA) supplemented with Halt protease inhibitor cocktail (Thermo Fisher Scientific) ...
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bioRxiv - Cancer Biology 2019Quote: ... immunoreactive proteins were detected by an enhanced chemiluminescence detection system (EMD-Millipore).
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bioRxiv - Immunology 2019Quote: ... The protein bands were visualized by Immobilon Western Chemiluminescent HRP Substrate (Millipore) according to the manufacturer’s instructions.
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bioRxiv - Microbiology 2019Quote: ... Protein concentration was determined using a microplate Bradford (Sigma –Aldrich Bradford Reagent) protein assay with bovine serum albumin standards.
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bioRxiv - Cell Biology 2020Quote: ... we use the PureProteome Protein G Magnetic Bead System (EMD Millipore Corp.). 1 mg protein extract was used for immunoprecipitation and incubated with protein G beads and 4 μg mouse anti-GFP antibody (B-2 #sc-9996 ...
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bioRxiv - Biochemistry 2019Quote: ... the reconstituted protein was mixed with 50 μM ADP-Ribose (Sigma-Aldrich) and further purified on a Superose 6 10/300 GL column (GE Healthcare ...
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bioRxiv - Cell Biology 2019Quote: ... proteins were visualized by staining with Amido Black staining solution (Sigma-Aldrich) according to manufacturer’s directions.
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bioRxiv - Genetics 2019Quote: ... and then captured by protein G-agarose/salmon sperm DNA (Millipore Sigma). Precipitated DNA was reverse cross-linked and then amplified by qPCR using primers amplifying amplicons with the following midpoints relative to the transcription start site of Shox2 ...
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bioRxiv - Biochemistry 2019Quote: ... whole cell lysates (WCLs) were precleared using protein A Sepharose (Sigma-Aldrich) for 30 minutes at 4°C ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... the protein samples were treated with 10 mM dithiothreotol (DTT) (Sigma, MO) at 56 °C for 1 h and 55 mM IAA (Sigma ...
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bioRxiv - Plant Biology 2019Quote: ... 0.2% Igepal CA-630 and Protein Inhibitor Cocktail (Sigma-Aldrich; http://www.sigmaaldrich.com/). The cell suspension was frozen in liquid nitrogen and then thawed on ice and centrifuged for 5 min at 150 g ...
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bioRxiv - Microbiology 2020Quote: ... and proteins were purified over HIS-Select (Sigma–Aldrich, St. Louis, MO) nickel affinity resin in 20 mM Tris (pH 8.0 ...
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bioRxiv - Biochemistry 2019Quote: ... Proteins were affinity purified by anti-Flag M2 affinity resin (Sigma-Aldrich), which followed digestion of affinity tag and deglycosilation by TEV protease and MBP-fusion endoglycosidase (New England Biolabs) ...
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bioRxiv - Plant Biology 2020Quote: ... and proteins were detected after blotting with anti-FLAG M2 antibody (SIGMA) (1:10,000 dilution ...
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bioRxiv - Cell Biology 2019Quote: ... Proteins were visualized by ECL (Enhanced chemiluminescence; Merck Millipore, Cat. No. WBKLS0500). The signal was detected using Vilber FUSION-SL system ...
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bioRxiv - Genetics 2020Quote: ... Protein extraction was carried out on ice using RIPA buffer (Sigma Aldrich) supplemented with protease inhibitor cocktail (Sigma) ...
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bioRxiv - Genomics 2021Quote: ... complexed with 6 mg pre-swollen protein A Sepharose beads (Sigma-Aldrich) during a 2-h incubation at 4°C ...
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bioRxiv - Genetics 2020Quote: ... and proteins were eluted using 100 µg/mL 3x FLAG peptide (Sigma). Eluates were analysed by western blotting with anti-FLAG antibodies (1:5000 ...