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Citations for Millipore Sigma :
8451 - 8500 of 10000+ citations for Rat Epithelial membrane protein 1 EPN3 ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2021Quote: ... Protein bands were visualized with Immobilon Chemiluminescent Alkaline Phosphatase substrate (Millipore) and detected in a VersaDoc Model 4000 CCD camera system (BioRad) ...
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bioRxiv - Molecular Biology 2021Quote: ... Bradford dye for protein estimation was purchased from Sigma-Aldrich (USA). All the primers were synthesized by GCC Biotech (India ...
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bioRxiv - Microbiology 2021Quote: ... Protein samples were spiked with ubiquitin (Sigma-Aldrich BioUltra, 5 nmol), a small subunit protein native to eukaryotes ...
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bioRxiv - Biophysics 2021Quote: ... 2.5μL of Precision Plus Protein™ Dual Color Standard from Sigma was used.
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bioRxiv - Biochemistry 2020Quote: ... proteins were buffer exchanged in centrifugal concentrators (Amicon Ultra, EMD Millipore) to buffer A plus 300 mM NaCl and 20 mM imidazole ...
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bioRxiv - Neuroscience 2020Quote: ... Proteins were reconstituted in sterile DMSO (Millipore-Sigma, Burlington, MA, USA) to stock concentrations of 10 mM ...
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bioRxiv - Cell Biology 2022Quote: ... Protein extracts were mixed with 2x Laemlli sample buffer (S3401, Sigma) and heated at 95°C for 10 minutes prior to SDS-PAGE ...
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bioRxiv - Cell Biology 2022Quote: Protein lysates were collected using Radio-Immunoprecipitation Assay (RIPA) buffer (Sigma), supplemented with protease inhibitor (Roche ...
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bioRxiv - Cell Biology 2022Quote: ... After protein quantification by the bicinchoninic acid colorimetric assay system (Sigma), 1mg protein samples were used for HDAC3 immunoprecipitation using four microliters of HDAC3 antibodies (Cell Signalling ...
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bioRxiv - Biochemistry 2022Quote: ... the proteins were digested with trypsin (TPCK Treated; Sigma; Cat. # 4352157). iTRAQ labeling of the peptides in each sample was performed using the iTRAQ Reagents Multiplex kit (AB Sciex) ...
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bioRxiv - Biochemistry 2022Quote: ... The protein was concentrated in a 50,000 MWCO Amicon Concentrator (Millipore) to <1 mL and injected onto a Superdex™ 200 10/300 GL Increase size-exclusion column (GE Healthcare ...
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bioRxiv - Biochemistry 2022Quote: ... The eluted protein was treated with 10 U PreScission protease (Sigma) at 4°C for 2 hours ...
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bioRxiv - Biochemistry 2022Quote: ... the proteins were concentrated using a 10 K Amicon (Merck-Millipore), flash-frozen in liquid nitrogen and stored at −80°C until use.
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bioRxiv - Cancer Biology 2022Quote: ... Antibodies were recovered by binding to protein A/G agarose (Millipore), and the eluted DNA fragments were used directly for qPCR or subjected to high-throughput sequencing (ChIP-Seq ...
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bioRxiv - Cell Biology 2022Quote: ... and phosSTOP if phosphorylated proteins were being investigated (Sigma-Aldrich, 04906845001)] ...
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bioRxiv - Microbiology 2022Quote: ... HA-tagged proteins were immunoprecipitated with anti-HA agarose beads (Sigma) and processed for Western blotting as previously described.
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bioRxiv - Microbiology 2022Quote: ... affinity purified IcsAp protein was mixed with 0.1 mM DSP (Sigma) in PBS and incubated at room temperature for 30 min ...
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bioRxiv - Synthetic Biology 2022Quote: ... resuspended and lysed in 200 μL Bugbuster protein extraction reagent (Millipore). Following another centrifugation ...
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bioRxiv - Plant Biology 2022Quote: ... samples were incubated with 25 μl of protein A beads (Millipore) for 2 h in cold room (100 μL were collected as input) ...
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bioRxiv - Neuroscience 2022Quote: ... Resulting protein lysates were precipitated with trichloroacetic acid (TCA) (Sigma, T0699). Briefly ...
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bioRxiv - Plant Biology 2023Quote: ... Protein extracts were then reduced with 10 mM dithiothreitol (D9779, Sigma), followed by alkylation with 30 mM Iodoacetamide (I1149 ...
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bioRxiv - Physiology 2022Quote: ... Target protein expression was normalized to β-actin (Sigma-Aldrich, #2066) or vinculin (Bio-Rad ...
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bioRxiv - Molecular Biology 2022Quote: ... the supernatant was pre-cleared with Protein G agarose beads (Sigma) and anti-rabbit IgG (Sigma) ...
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bioRxiv - Microbiology 2022Quote: ... were firstly incubated with the Protein-G agarose (Millipore, 16-266) for 2 hrs ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... Protein was concentrated using a 10-kDa Amicon filter device (Millipore) and aliquoted into 1 mg aliquots for further use.
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bioRxiv - Physiology 2022Quote: ... to standardize protein loading in Laemmli 2X Concentrate sample buffer (Sigma) and deionized water for gel electrophoresis (Bio-Rad) ...
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bioRxiv - Physiology 2022Quote: ... Total protein was quantified with Bradford reagent (SIGMA, cat. no. B6916) and 25 μg of extract were suspended 1:6 (v/v ...
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bioRxiv - Systems Biology 2022Quote: ... Protein was visualized with Immobilon Western Chemiluminescent HRP Substrate (Millipore; #WBKLS0500) and imaged on a ChemiDoc (BioRad) ...
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bioRxiv - Bioengineering 2023Quote: ... protein was extracted by using RIPA lysis buffer (20-188, Millipore) supplemented with cOmplete Mini protease and phosphatase inhibitors tablets (Roche) ...
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bioRxiv - Biophysics 2024Quote: ... The protein was concentrated with Amicon Ultra-15 centrifugal filters (Millipore) and buffer exchanged to storage buffer (20 mM Tris-HCl ...
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bioRxiv - Genomics 2024Quote: ... The protein concentration was measured with the Bradford Assay (Sigma-Aldrich). Aliquots of the extracts were separated by sodium dodecyl sulphate-polyacrylamide gel electrophoresis and transferred onto polyvinylidene difluoride membranes (Amersham/GE Healthcare) ...
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bioRxiv - Biochemistry 2024Quote: ... Protein was concentrated using Amicon Ultra centrifugal filter units (Merck Millipore). Protein was then aliquoted ...
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bioRxiv - Cancer Biology 2023Quote: ... Proteins in the gel bands were reduced with dithiothreitol (DTT) (Sigma) and alkylated by iodoacetamide (IAA ...
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bioRxiv - Biochemistry 2024Quote: ... Eluted protein was concentrated using Amicon Ultra Centrifugal Filter units (Millipore) and further purified by size exclusion chromatography using a Superdex 75 prepgrade column (GE Healthcare ...
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bioRxiv - Biochemistry 2024Quote: ... The protein was blotted off with filter paper (Sigma–Aldrich, WHA1001110), and immediately after blotting ...
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bioRxiv - Cancer Biology 2024Quote: ... Proteins then transferred to 0.45 µM Immobilon-FL PVDF (Millipore Sigma) after methanol activation ...
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bioRxiv - Neuroscience 2024Quote: ... Primary antibodies and biotinylated Hyaluronic Acid Binding Protein (HABP, #385911 Millipore) were incubated for 72 hours at 4°C or 24 hours at room temperature ...
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bioRxiv - Physiology 2024Quote: ... Protein was visualized with Immobilon Western Chemiluminescent HRP Substrate (Millipore, #WBKLS0500) and imaged on a ChemiDoc (BioRad) ...
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bioRxiv - Molecular Biology 2024Quote: ... The proteins bound to anti-Flag M2 affinity beads (Sigma-Aldrich) were recovered from the supernatants and washed by Flag wash buffer (50 mM NaPi (pH 7.5) ...
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bioRxiv - Cell Biology 2024Quote: ... and the protein concentration was determined with Bradford reagent (Sigma-Aldrich). Lysates were subsequently denatured in Laemmli sample buffer for 5 minutes at 95°C ...
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bioRxiv - Synthetic Biology 2023Quote: ... and pellets were resuspended in BugBuster® protein extraction reagent (Millipore). Protein from the lysed cell suspension was then extracted by chloroform/methanol precipitation ...
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bioRxiv - Immunology 2023Quote: ... FLAG-tagged proteins were immunoprecipitated with anti-FLAG M2-agarose (Sigma), following the manufacturer’s instructions.
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bioRxiv - Cancer Biology 2023Quote: ... Protein concentration was determined by a Bradford assay (Sigma Aldrich; B6916). Proteins were denatured in NuPAGE™ LDS Sample Buffer (4X ...
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bioRxiv - Biochemistry 2023Quote: The lysate was pre-cleared with ∼20µL Protein G agarose (Sigma) per 1mL of lysate at 4°C for 30min ...
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bioRxiv - Biochemistry 2023Quote: ... Ubiquitinated proteins were detected using an anti-FLAG antibody (F7425, Sigma), HA-TAP-Gb1 and endogenous Gb1-4 were detected with anti-HA (16B112 ...
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bioRxiv - Immunology 2023Quote: ... injection of 30 μg of OVA protein (Grade V; Sigma-Aldrich) adsorbed to 2 mg of aluminum hydroxide (alum ...
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bioRxiv - Systems Biology 2023Quote: ... total protein amounts were assayed using Fast Green FCF (Sigma, #F7252) staining solution47 and imaged using LICOR Odyssey 700 nm ...
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bioRxiv - Microbiology 2023Quote: ... lysates were incubated with PureProteome protein A/G magnetic beads (Sigma) for 1-2 h ...
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bioRxiv - Microbiology 2023Quote: ... and then incubated with PureProteome protein A/G magnetic beads (Sigma) for 1 h ...
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bioRxiv - Neuroscience 2023Quote: ... along with several dilutions of a SOD1 protein standard (Sigma, S9697) were separated on native 12% Tris-Glycine acrylamide gels (ThermoFisher ...