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Citations for Millipore Sigma :
8551 - 8600 of 10000+ citations for Human Urocortin 3 UCN3 CLIA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2024Quote: ... reconstructed from a clonotypic plasma blast obtained from the cerebrospinal fluid of a AQP4-IgG-positive NMOSD patient52 together with human complement (15-30 U/ml, Sigma-Aldrich, #S1764) was injected using a custom-made microinjector and a pulled glass capillary (Drummond PCR micropipettes ...
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bioRxiv - Neuroscience 2024Quote: Human brain pericytes were cultured on a glass coverslip coated with 10 mg/mL of poly-L-lysine (Millipore Sigma, Cat# P8920). Cells were fixed and permeabilized with 4% paraformaldehyde for 15 min ...
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bioRxiv - Bioengineering 2024Quote: Recombinant active human MMP-9cd (auto-cleavage resistance) 34 with an N-terminal His6x tag was expressed in Rosetta™(DE3) pLysS (Millipore Sigma) cells using a pET-28a(+)-His6x-MMP-9cd vector as previously described 33,35,36 ...
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bioRxiv - Neuroscience 2021Quote: ... membranes were incubated with primary antibodies (Table 3) diluted in Intercept (TBS) blocking buffer with 0.05% Tween-20 (Sigma-Aldrich) overnight at 4°C on an orbital shaker.
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bioRxiv - Microbiology 2020Quote: ... cells were washed 3 times with PBS before adding fresh post-infection media containing 1% microcrystalline cellulose (Avicel, Sigma Aldrich). Cells were further incubated at 37 °C for 24 h ...
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bioRxiv - Plant Biology 2019Quote: ... auxin treatments were applied by spraying bolting Col-0 and ett-3 inflorescences with a solution containing 100 µM IAA (Sigma) or cvxIAA and 0.015% Silwet L-77 (De Sangosse Ltd.) ...
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bioRxiv - Biophysics 2021Quote: ... The 30-40 mM ligand stock solutions were prepared in d6-DMSO from the crystalline solids (Phorbol-12,13-dibutyrate, Prostratin, and Ingenol-3-angelate, all from Sigma-Aldrich→ ...
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mTOR inhibition enhances delivery and activity of antisense oligonucleotides in uveal melanoma cellsbioRxiv - Cancer Biology 2021Quote: ... cells were washed 3 times with PBS and permeabilized at room temperature for 15 min with 0.1% Triton X-100 (Sigma Aldrich). Next ...
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bioRxiv - Biophysics 2020Quote: ... the following target sequences were used: 5’-GCTTCAGGATTCAATGCCATGG-’3 (#1) using the all-in-one CRISPR/Cas9 plasmid (Sigma-Aldrich) followed by cell-sorting for GFP expression to generate single cell clones with disrupted ANXA4 reading frame.
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bioRxiv - Biophysics 2020Quote: ... supplemented with 3 mM CaCl2 and treated with 10 µM A23187 calcium ionophore (Sigma-Aldrich, Inc., St. Louis, MI, USA) for 60 min at room temperature.
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bioRxiv - Cancer Biology 2021Quote: ... the plates were cultured for 2-3 weeks in the incubator at 37°C with 5% CO2 before being stained with 1% crystal violet reagent (Sigma) and counted.
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bioRxiv - Microbiology 2020Quote: ... Hereafter the samples were digested for 3 h – overnight in digestion buffer (50 mM Tris pH 8.0, 1 mM EDTA (Sigma, ED2P), 0.5 % Triton X-100 (Thermo Fisher ...
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bioRxiv - Immunology 2020Quote: ... and S100A9 (rA9) were concentrated using an Amicon Ultra-4 centrifugal filter device with a 3 kDa molecular-weight cutoff (Millipore). The primary sequence ...
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bioRxiv - Molecular Biology 2020Quote: Aliquots of 3 μg of GST-LC3B (Viva Bioscience) were incubated alone or with 250 ng of His-STK4 (Millipore) in in vitro kinase reaction buffer (10 mM MgCl2 ...
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bioRxiv - Cell Biology 2020Quote: ... the samples containing N-cTnC were pooled and concentrated using Amicon centrifugal concentrators with a 3 KDa molecular weight cut-off (Millipore).
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bioRxiv - Cell Biology 2020Quote: ... The HCT116 cells were transfected with PX462-C1C2 using Lipofectamine 2000 and cultured in the presence of puromycin (3 μg/mL, Sigma) for 48 hr after post-transfection 24 hr ...
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bioRxiv - Cell Biology 2020Quote: ... membranes were washed once with PBST and then incubated with the appropriate primary antibody diluted in PBST + 3% (w/v) BSA (Sigma) for 2 hours at RT ...
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bioRxiv - Cell Biology 2020Quote: ... These slides were rehydrated in 1x PBS at room temperature for 15 min followed by blocking for 1 hour in 1x PBS containing 3% BSA (Bovine serum albumin, Sigma). The slides were then incubated for 1 hour at room temperature with 1xPBS+3% BSA with respective antibodies ...
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bioRxiv - Cell Biology 2020Quote: ... and Ypt1-7xHis were mixed in a 1:3 molar ratio and incubated overnight at 4°C with calf intestinal alkaline phosphatase (Sigma) to hydrolyze nucleotide ...
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bioRxiv - Cell Biology 2020Quote: ... Anti-phosphotyrosine (anti-pY) clone 4G10 and anti-facilitative glucose transporter 3 (anti-GLUT3) AB1344 from Millipore (Temecula, CA, USA); anti-phospho PKA substrates (anti-pPKAs ...
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bioRxiv - Cell Biology 2020Quote: Every fish in this study was euthanized by supplying 1.5 g/L of ethyl 3-aminobenzoate methanesulfonate (MS-222, E10521, Sigma-Aldrich) to the fish tank water ...
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bioRxiv - Cell Biology 2020Quote: ... GV oocytes were cultured in M2 medium supplemented with 0.2 mM of the phosphodiesterase inhibitor 3-Isobutyl-1- methylxanthine (IBMX, Sigma-Aldrich) at 37 °C ...
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bioRxiv - Cell Biology 2020Quote: ... the pronuclei were transferred to 0.2 ml PCR tubes in 3 μl sample buffer covered with mineral oil (Sigma-Aldrich) and were decrosslinked at 65°C overnight ...
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bioRxiv - Genetics 2021Quote: ... all diluted in a 1:1 mixture of PBT and 3% BSA: mouse anti-α-tubulin (1:500, Sigma T6199), rabbit anti-Spd-2 (1:4.000 ...
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bioRxiv - Genetics 2021Quote: ... Medium was changed after 3 days and cells were selected in FM with 5μg/ml Puromycin (Sigma-Aldrich, cod. P8833) for 3 days ...
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bioRxiv - Biochemistry 2020Quote: Methylene blue treatments RBCs from WT and band 3 KO mice were incubated with methylene blue (100 uM, Sigma Aldrich) at 37°C for 1h ...
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bioRxiv - Molecular Biology 2020Quote: ... After two washing with 3% BSA in PBS 1X cells were permeabilized with 0.5% Triton® X-100 (Sigma Aldrich) and incubated for 15 minutes at room temperature ...
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bioRxiv - Microbiology 2019Quote: Methylcobalamin (Vitamin B12 CAS Number 13422-55-4) and L-methionine (CAS Number 63-68-3) were purchased from Sigma and dissolved in water at the highest possible soluble concentrations to prepare stock solution ...
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bioRxiv - Microbiology 2019Quote: A sample (10 mL) was removed from the Gambierdiscus culture and filtered through 3-μm membrane (Merck Millipore, Darmstadt, Germany). One hundred microliters of the filtrate ...
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bioRxiv - Developmental Biology 2021Quote: ... 1% ITS-X supplement (WAKO) and purified BSA (3%, WAKO)) and N2B27 (DMEM/F12/GlutaMax + Neurobasal at 1 : 1 (Sigma), 1 × B27 (Gibco) ...
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bioRxiv - Evolutionary Biology 2020Quote: ... Both fins of each genotyped larva were dissected and transferred to a drop of Methyl Cellulose 3% (M0387, Sigma-Aldrich) on a slide for documentation using an Olympus SZX16 (Model SZX2-ILLB ...
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bioRxiv - Developmental Biology 2020Quote: ... and then incubated with nitro blue tetrazolium chloride/5-bromo-4-chloro-3-indolyl phosphate (NBT/BCIP) substrates (Sigma-Aldrich) in NTM (100mM NaCl ...
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bioRxiv - Biochemistry 2020Quote: ... and the blotting signals were chemically visualized with either the nitro-blue tetrazolium/5-bromo-4-chloro-3′-indolyphosphate (NBT/BCIP) chromogenic assay (Sigma) or infrared scanner ...
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bioRxiv - Microbiology 2022Quote: ... embryos were mechanically dechorionated if necessary and anaesthetized in 0.02 % (w/v) buffered 3-aminobenzoic acid methyl ester (pH 7.0) (Tricaine; Sigma-Aldrich, A5040). The bacterial suspension was then injected into the hindbrain ventricle of casper zebrafish larvae at 2 dpf ...
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bioRxiv - Plant Biology 2022Quote: ... We used an Ascentis Express C-18 Column (3 cm × 2.1 mm, 2.7 µm) (Sigma-Aldrich, St. Louis, MO, USA) connected to an API 3200 LC-MS/MS (Sciex ...
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bioRxiv - Microbiology 2022Quote: ... pneumoniae was grown at 37°C on LB agar plates supplemented with 3 μg/mL potassium tellurite (Sigma-Aldrich, USA).
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bioRxiv - Microbiology 2022Quote: ... the samples were permeabilized with TritonX-100 at 0.1% in D-PBS with subsequent blocking with 3% Bovine Serum Albumin (Sigma-Aldrich) in D-PBS ...
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bioRxiv - Neuroscience 2021Quote: ... Sections were then washed 3 x 5 min in PBS and nuclei stained with Hoechst 3342 (10 µg/ml in PBS, Sigma) for 20 min at room temperature ...
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bioRxiv - Molecular Biology 2021Quote: Adult male and female worms were relaxed and separated by incubation (15 min) in a 0.25% solution of the anaesthetic ethyl 3-aminobenzoate methanesulphonate (Sigma-Aldrich) dissolved in DMEM ...
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bioRxiv - Neuroscience 2021Quote: ... the NMDA glutamate receptor antagonist [3H]3-(2-carboxypiperazin-4-yl) propyl-1-phosphonic acid (CPP, 10 μM, Sigma-Aldrich) and a selective group III metabotropic glutamate receptor agonist ...
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bioRxiv - Molecular Biology 2020Quote: ... The quality of nuclear and cytoplasmic extraction was validated by western blotting by α-Histone 3 (Sigma, St. Louis, MO) (1:2000 ...
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bioRxiv - Molecular Biology 2021Quote: ... Human bronchial epithelial cells (16HBEo-) were transduced with VSV-G pseudo-typed lentiviral particles in the presence of 3 μg/ml polybrene (Millipore). Expression vectors encoding the spike protein cDNA were directly transfected into 16HBEo− with Lipofectamine P3000 according to manufacturer’s recommendations (Invitrogen) ...
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bioRxiv - Molecular Biology 2021Quote: ... Beads were then washed 4x with Buffer A containing only 0.1% NP-40 and bound protein was eluted by 3 sequential incubations in PBS containing 0.1% triton x-100 (Sigma-Aldrich) and 0.5mg/ml of 3x FLAG peptide (Sigma-Aldrich ...
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bioRxiv - Biophysics 2022Quote: ... The clean coverslips were silanized by incubating in a solution of 2% (v/v) 3-aminopropyl-triethoxysilane (Sigma-Aldrich A3648) in acetone for 30 minutes ...
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bioRxiv - Biophysics 2022Quote: ... followed by buffer exchange (5 cycles of concentration to 0.5 ml volume and dilution with 5 ml buffer B) using a 3 kDa MWCO Amicon Ultra-15 centrifugal ultrafilter (Millipore), and the purified Alexa647-CAP was stored in aliquots at −80°C.
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bioRxiv - Cell Biology 2022Quote: ... Arteries were then stimulated with increasing concentrations of serotonin (5-HT, from 10 -8 to 3· 10 -5 M, Sigma), endothelium-dependent vasodilator acetylcholine (ACh ...
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Prom1 and Notch regulate ciliary length and dynamics in multiciliated cells of the airway epitheliumbioRxiv - Cell Biology 2022Quote: ... mTEC were cultured from ALI day -3 to ALI day 8 with an added gamma-secretase inhibitor (DAPT, 50 μM, Sigma) or vehicle control (DMSO ...
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bioRxiv - Immunology 2022Quote: ... Plates were washed once with PBS containing 0.1% Tween 20 (PBS-T) and blocked with 3% bovine serum albumin (BSA, Sigma Aldrich) in PBS-T for 1 hr at room temperature ...
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bioRxiv - Genomics 2020Quote: BJ cells (Fig. 3) and A549 cells (Fig. S10) were treated with 5 μg/mL of Actinomycin D (Sigma, A9415), which inhibits transcription by halting elongation of the transcribed RNA (42 ...
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bioRxiv - Neuroscience 2020Quote: ... Staining with Cyanine dye 2 (Cy2)-conjugated streptavividin (Jackson Immuno Research, 1:400 in PBS, with 3% bovine serum albumin, Jackson Immuno Research and 0.05% sodium azide, Sigma-Aldrich) for 60 min was used to identify biocytin-filled cells ...