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Citations for Millipore Sigma :
8751 - 8800 of 10000+ citations for Human Urocortin 3 UCN3 CLIA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2022Quote: ... cells were incubated for 3 days in the presence of 100 μg/ml heparin from porcine intestinal mucosa (Sigma, H3393) to prevent LRP1-mediated endocytic clearance before harvesting ...
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bioRxiv - Biophysics 2022Quote: ... The recordings for PI(3, 5)P2 injection were performed under ND-96 solution containing 10 µM Ani9 (Sigma-Aldrich) to inhibit the endogenous Ca2+- activated Cl- channel ...
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bioRxiv - Biophysics 2022Quote: ... A negative control without tyrosinated tubulin was prepared by treating 3 mg/mL bovine tubulin with 0.02 mg/mL carboxypeptidase A (CPA, Sigma Aldrich) on ice for 15 min to proteolytically cleaved the C-terminal tyrosine of α-tubulin ...
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bioRxiv - Bioengineering 2022Quote: ... supplemented with CK+DCIR additives (3 μM CHIR99021 [CHIR; PeproTech], 10 ng/mL keratinocyte growth factor [KGF; PeproTech], 50 nM dexamethasone [Sigma] ...
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bioRxiv - Biochemistry 2022Quote: ... The column was washed with 30 column volumes (CV) of wash buffer before bound proteins were eluted with wash buffer supplemented with 3 mM D-desthiobiotin (Sigma). The protein was transferred into wash buffer without D-desthiobiotin and incubated overnight at 4°C with 1:100 (w/w ...
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bioRxiv - Biochemistry 2022Quote: ... The electrode was then immersed in 3 mL of a mixed buffer solution containing 5 mM of HEPES (Sigma Aldrich), 5 mM of MES (Sigma Aldrich ...
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bioRxiv - Biochemistry 2022Quote: ... 0.3% Triton X-100 in PBS) and with primary antibodies overnight at +4 °C (primary Abs: mouse anti-flag –Sigma F1804 ...
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bioRxiv - Genetics 2022Quote: ... Degradation of the AID-tagged RAD21 was induced with addition of 500 μM indole-3-acetic acid (auxin; Sigma-Aldrich). Degradation of AID-tagged WAPL was induced with addition of 1μM 5-Ph-IAA (modified auxin ...
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bioRxiv - Cancer Biology 2022Quote: ... supplemented with 1% protease inhibitor and phosphatase inhibitor cocktail 2 and 3 (all from Sigma Aldrich, St. Louis, MO, USA). Samples were centrifuged at 14,000 rpm for 20 minutes at 4°C ...
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bioRxiv - Cell Biology 2022Quote: The tissue section on the Stereo-seq chip (5 cm x 3 cm or 2 cm x 3 cm) was then incubated at 37°C for 5-8 min and subsequently fixed in methanol (Sigma, 34860 ...
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bioRxiv - Neuroscience 2021Quote: ... Plates were washed 3 times by adding and removing 300 μl of wash buffer to each well (PBS + 0.05%Tween-20; Sigma, UK) and incubated for 1 hour with blocking buffer (Reagent Diluent ...
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bioRxiv - Cell Biology 2021Quote: ... were freshly oxidized by oxygen plasma and then incubated in a solution composed of 0.3% w/v 3- (trimethoxysilyl)propyl methacrylate (Sigma Aldrich) and toluene (Fisher Scientific ...
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bioRxiv - Neuroscience 2020Quote: ... then blocked with 3 % BSA in PBS for 1 h at room temperature and incubated with primary antibodies (mouse anti-GFAP, Sigma, 1:500 ...
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bioRxiv - Microbiology 2020Quote: ... 3 days post-fertilization (dpf) zebrafish larvae were anaesthetized for 2 - 3 minutes in embryo medium E3 containing 0.2 mg/ml Tricaine (Sigma-Aldrich). Thereafter ...
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bioRxiv - Cancer Biology 2020Quote: ... membranes were washed once with PBST and then incubated with the appropriate primary antibody diluted in PBST + 3% (v/v) BSA (Sigma) for 2 hours at RT ...
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bioRxiv - Microbiology 2020Quote: ... the medium was replaced with MTT [3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide] (Sigma-Aldrich, St. Louis, MO, USA) final concentration at 0.5 mg/mL and incubated for 4 h at 37°C in a humidified incubator with 5% CO2 ...
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bioRxiv - Molecular Biology 2020Quote: ... embryos were cultivated at 28.5 °C for 3 days in fresh standard E3 medium supplemented with 0.05% methylene blue (Sigma Aldrich, MO) and 0.003% phenylthiourea (PTU ...
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bioRxiv - Microbiology 2021Quote: ... then washed and cultured one week in normal cell culture medium and passaged in 10 cm dishes seeded with 3×105 cells using culture medium supplemented with 30 μM 6-thioguanine (6-TG, Sigma). Cells were also plated without 6-TG to determine plating efficiency ...
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bioRxiv - Microbiology 2021Quote: ... Immunoreactive bands were detected by the addition of BCIP (5-bromo-4-chloro-3-indolylphosphate)-nitroblue tetrazolium solution (Sigma-Aldrich). The reaction was stopped after 2 min by washing the blots with large volumes of deionized water.
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bioRxiv - Neuroscience 2021Quote: ... The labeled samples were washed 3 times in PBT (10 min each) and incubated with DAPI (Sigma, cat. N. D9542) (5μg/ml ...
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bioRxiv - Cell Biology 2020Quote: ... were blocked for 1h in TBS containing 0.1% Tween-20 (TBS-T) supplemented by 3% fatty-acid free BSA (Sigma Aldrich) before incubation with the purified STARD10 protein (1 μg/mL in TBS-T + 3% BSA ...
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bioRxiv - Neuroscience 2020Quote: ... Tg[elavl3:Gal4;10xUAS:jRGECO1b] at 3 dpf were paralyzed by a 5-min bath application of 1 mg/ml a-bungarotoxin (Sigma, 203980). Larvae were mounted on their side in a field stimulation chamber (Warner ...
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bioRxiv - Neuroscience 2021Quote: Astrocyte cultures were fixed with 4% PFA in PBS (pH 7.4) for 15 min and nonspecific sites were blocked with 3% bovine serum albumin (BSA; Sigma-Aldrich), 5% normal goat serum (Sigma-Aldrich ...
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bioRxiv - Neuroscience 2021Quote: ... larvae were anesthetized with 0.2 mg/mL ethyl-3-aminobenzoic acid ethyl ester (MESAB, Sigma-Aldrich E10521, St. Louis, MO) and mounted laterally in 2% low-melting temperature agarose (Thermo Fisher Scientific 16520) ...
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bioRxiv - Neuroscience 2021Quote: ... next permeabilized for 20 minutes in PBST (PBS with 0.3% Triton X-100; BioShop) and blocked for 2 h in 3% BSA (Sigma-Aldrich) in PBST at RT ...
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bioRxiv - Physiology 2021Quote: ... was incubated for 2-3 h at 4 °C with 30 µl of FLAG-conjugated beads (anti-DYDDDDDK beads, Sigma) or empty beads (ThermoScientific) ...
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bioRxiv - Physiology 2021Quote: ... cells were differentiated into adipocytes using MDI medium (100 mg/ml 3-isobutyl-1-methylxanthine, 100 ng/ml dexamethasone and 1 mg/ml insulin, both from Sigma) for 4 days ...
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bioRxiv - Microbiology 2020Quote: ... The plasma-treated coverslips were then transferred into 150 mL of 1% (v/v) (3-Aminopropyl)triethoxysilane (APTES) (Sigma-Aldrich) in toluene (Sigma-Aldrich ...
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bioRxiv - Microbiology 2020Quote: ... The media was further concentrated to ~3 ml with a 100 kDa cut-off centrifugal concentrator filter (Amicon Ultra, Merck Millipore) and layered onto a 20-60% w/v sucrose density gradient in PBS buffer ...
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bioRxiv - Immunology 2020Quote: Supernatants from epithelial cells were centrifuged at 5000g for 1 hr at 4 °C and the residual volume collected using 3 kDa molecular weight cut off 50 mL or 15 mL tube filters (Millipore). The lower volume was collected and volume measured then the residual volume from the upper level was diluted to a similar volume to the lower fraction ...
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bioRxiv - Neuroscience 2021Quote: Adult mosaic zebrafish (F0) were anesthetized in pH-buffered 0.2 mg/ml ethyl 3-aminobenzoate methanesulfonate solution (MS-222, Sigma-Aldrich). The caudal fin (2 mm of the end ...
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bioRxiv - Biochemistry 2021Quote: ... Crystals occur over the course of 3 days in drops where the reservoir contained 100 mM Hepes pH 7.5 (Sigma-Aldrich), 1% v/v 2-methylpentanediol (MPD ...
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bioRxiv - Bioengineering 2021Quote: ... slides were either treated with SIGMA FAST™ BCIP/NBT (5-Bromo-4-chloro-3-indolulphosphate/Nitro blue tetrazolium, pH 9.5, Sigma) and counterstained with nuclear fast red (Sigma ...
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bioRxiv - Neuroscience 2020Quote: ... The phosphorylated peptides were released from IMAC beads by 3 × 100 μL 500 mM dibasic sodium phosphate (Na2HPO4, Sigma, S9763), pH 7.0 ...
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bioRxiv - Cancer Biology 2020Quote: ... 10 µl of a solution of 5 mg/mL MTT [3-(4,5)-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide] (Sigma Chemical Co.) was added ...
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bioRxiv - Cancer Biology 2020Quote: ... Beads were washed with TBS-lysis buffer and proteins were eluted from beads by addition of 3×Flag peptides (150 μg/mL, Sigma) for 1 h at 4°C and then subjected to tryptic digestion followed by LC-MS/MS ...
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bioRxiv - Neuroscience 2021Quote: ... the right hind paw of mice was inflamed by injection of 20 µl 3% (w/v) λ-carrageenan (Sigma-Aldrich), inflammation was induced while mice were under inhalation anesthesia (2% isoflurane) ...
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bioRxiv - Neuroscience 2021Quote: ... a DNA fragment encoding AviTag-GluN1(834-863) was synthesized by the Integrated DNA Technologies (Table 3) and inserted into pET-28a vector (Novagen) using NdeI and XhoI sites.
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bioRxiv - Biochemistry 2020Quote: ... Day 1 adult animals were mounted on 2% (vol/vol) agar pads and immobilized with 30 mg/mL 2-3-butaneione monoxime (BDM, Sigma) in M9 buffer.
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bioRxiv - Biochemistry 2020Quote: ... animals were mounted on 2% (vol/vol) agar pads and immobilized in 30 mg/mL 2-3-butaneione monoxime (BDM, Sigma) in M9 buffer ...
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bioRxiv - Biophysics 2021Quote: ... cells were treated for 10 min with a solution of 0.25% Triton-X100 in blocking buffer (solution of 3% bovine serum albumin (BSA, Sigma-Aldrich) in BRB80 buffer) ...
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bioRxiv - Cancer Biology 2021Quote: ... PC-3 cells were transfected with either the control or Sirt5 CRISPR/Cas9 plasmids according to the manufacturer’s instructions (Sigma-Aldrich). The transfection efficiency was determined by a green fluorescent protein (GFP ...
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bioRxiv - Biophysics 2021Quote: ... and 1,2-dioleoyl-sn-glycero-3-phospho-L-serine (sodium salt) 18:1 PS (DOPS) was purchased from Sigma-Aldrich. N-((6-biotinoyl)amino)hexanoyl)-1,2-dihexadecanoyl-sn-glycero-3-phosphoethanoamine ...
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bioRxiv - Neuroscience 2022Quote: ... the labeled SPs or anti-ACE2 antibody were purified using 3 kDa molecular weight cut-off ultrafiltration filter (Amicon Ultra Centrifugal Filter, Millipore). There was no detectable dye in the filtrate.
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bioRxiv - Genetics 2022Quote: ... Slides were then rinsed in washing buffer five times for 3 min each and nuclei were stained with 4lr,6-diamidino-2-phenylindole (DAPI, cat # D-1388, Sigma) at a concentration of 1 μg/ml for 10 min at RT ...
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bioRxiv - Microbiology 2022Quote: ... Calu-3 cells media was instead supplemented with a 1x final concentration of non-essential amino-acids (Sigma cat. M7145) and sodium pyruvate (Gibco cat ...
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bioRxiv - Neuroscience 2022Quote: ... incubated for 30 minutes on a plate shaker at 37 °C with shaking, followed by addition of 3 μL of final 2.3 mM phenylmethylsulfonyl fluoride (PMSF, a serine hydrolase inhibitor) (Sigma Aldrich) diluted in PBS/isopropanol (Sigma Aldrich ...
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bioRxiv - Immunology 2022Quote: ... A second cohort of SARS-CoV-2-infected male hamsters were intravenously co-injected with 18F-FES and 0.3 mg/kg of E2 (3% DMSO; Sigma-Aldrich) on day 7 post-infection (n = 4) ...
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bioRxiv - Cell Biology 2022Quote: ... The cell suspension was spun at 1000g for 3 minutes and re-suspended in islet media-G11 mixed with concentrated lentivirus and DEAE-Dextran hydrochloride (Sigma Millipore). The mixture was loaded to the GravityPLUS hanging drop 96-well microplate (InSphero ...
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bioRxiv - Biochemistry 2022Quote: ... Loss of Memo was induced by 3 daily intraperitoneal injections with 2mg tamoxifen at age 4 weeks (T5648 Sigma-Aldrich, distributed through Merck ...