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Citations for Millipore Sigma :
8401 - 8450 of 10000+ citations for Human Urocortin 3 UCN3 CLIA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Bioengineering 2023Quote: ... they were incubated with 4 mg/mL of N-(3-Dimethylaminopropyl)-N′-ethylcarbodiimide hydrochloride (EDC; Sigma-Aldrich, Schnelldorf, Germany) dissolved in 100 mM 2-(N-morpholino ...
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bioRxiv - Biophysics 2023Quote: ... washed 3×20min with milli-Q water and stained overnight with Coomasie Brillant Blue (G250, Sigma, Saint-Louis, USA). They were finally rinced 3×20 min with milli-Q water.
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bioRxiv - Microbiology 2023Quote: ... Zebrafish larvae were anesthetized with egg water containing 0.02% buffered 3-aminobenzoic acid ethyl ester (Tricaine, Sigma-Aldrich, Netherlands) for bacterial infection and imaging experiments.
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bioRxiv - Microbiology 2023Quote: ... permeabilised with 0.1% Triton X-100 in PBS for 10 minutes and blocked in 3% BSA (Sigma Aldrich, #A9418) for 1 hour at RT ...
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bioRxiv - Neuroscience 2023Quote: ... slices were then blocked and permeabilized in 3% BSA in PBS containing 0.1% Triton X-100 (Sigma-Aldrich # T8787) and stained with rabbit anti-GFP (2 μg/mL ...
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bioRxiv - Biophysics 2023Quote: ... The supernatant was mixed at a 3:2 ratio with a crowding buffer [20% dextran (from Leuconostoc spp., Mr 450,000-650,000; Sigma 31392) and 1 mM DTT in 50 mM Tris-HCl pH 7.4] ...
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bioRxiv - Neuroscience 2023Quote: Estrus was induced in ovariectomized females with a subcutaneous injection of 10 µg β-estradiol 3-benzoate (E2) (Sigma) in 0.05 mL sesame oil ∼48 hours before testing ...
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bioRxiv - Cancer Biology 2023Quote: ... or 250 nM Compound 3 were fixed and stained for mouse anti-γ-tubulin 1:500 (Sigma Aldrich, T5326), rabbit anti-KIF18A 1:100 (Bethyl ...
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bioRxiv - Plant Biology 2023Quote: ... The negative masters were treated with a solution of 3-aminopropyltriethoxysilane (015-28251, Sigma-Aldrich, St. Louis, MO, USA) overnight under ambient conditions once before use ...
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bioRxiv - Plant Biology 2023Quote: ... The resulting transformants were spotted on SD (−Leu, −Trp) and SD (−Trp, −Leu, −His) selection media containing different concentrations of 3-amino-1,2,4-triazole (Sigma, A8056) as previously reported 21 ...
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bioRxiv - Cell Biology 2023Quote: ... Pooled bone marrow stromal cells (BMSCs) were washed with 3 times with PBS and cultured in αMEM (Sigma, M2279) supplemented with 20% FBS ...
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bioRxiv - Microbiology 2023Quote: ... the bottom channel of a polydimethylsiloxane (PDMS) device was precoated with fibronectin (3 μg/ml, Sigma-Aldrich, Cat# F1141). The microfluidic device was generated according to our previous report65 ...
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bioRxiv - Neuroscience 2023Quote: ... A 1-in-3 series was collected directly onto gelatin-coated slides and Nissl-stained (cresyl violet, Sigma-Aldrich) for verification of electrode location.
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bioRxiv - Microbiology 2023Quote: ... Calu-3 cells (ATCC, HTB-55) were maintained in Eagle’s minimum essential medium (EMEM) (Sigma-Aldrich, Cat# M4655-500ML) containing 10% FBS and 1% PS ...
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bioRxiv - Molecular Biology 2023Quote: ... in PBS for 10 min at room temperature in the dark and washed 3 times with PBS (#D8537, Sigma). PBS was completely removed and cells were left to dry for 5 min at room temperature ...
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bioRxiv - Biochemistry 2023Quote: ... Proteins were eluted with 200 µL assay buffer supplemented with 0.6 mg∕mL 3× Flag® peptide (Sigma Aldrich), and analysed by Western blotting.
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bioRxiv - Microbiology 2023Quote: ... and the metal-loaded polymer purified and washed in C-buffer using an Amicon Ultra-0.5 centrifugal filter unit with 3 kDa cutoff (Millipore-Sigma). At the same time ...
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bioRxiv - Neuroscience 2023Quote: ... in 0.4% Triton X-100 and 2% NHS PBS solution during 3 h and then coverslipped with Mowiol (Sigma). After confocal imaging ...
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bioRxiv - Cell Biology 2023Quote: ... and microtubules (1 µM paclitaxel, T7402, Sigma Aldrich; 33 µM nocodazole, M1404, Sigma Aldrich, 3 different commercial lots tested). The effect of nocodazole during overnight/short-term culture was enhanced by 10 µM verapamil (V4629 ...
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bioRxiv - Microbiology 2023Quote: Infected S10-3 cells seeded onto coverslips were fixed in 4% PFA and permeabilized in 0.1% Trion-X100 (Sigma). For co-detection of RNA and capsid ...
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bioRxiv - Immunology 2023Quote: ... A total of 500 μL of normalized culture was mixed with 100 μL of 1% Zwittergent 3-14 (Sigma) in triplicate in 100 mM citric acid and incubated at 50 °C for 20 min ...
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bioRxiv - Immunology 2023Quote: ... Cells were incubated on ice for 3 min and 2 mL wash buffer (10 mM Tris-HCl, 10 mM NaCl, 3 mM MgCl2, 0.1 % Tween-20, 1 % BSA, 1 mM DTT, 1 U/µL Sigma Protector RNase inhibitor in nuclease free water ...
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bioRxiv - Genomics 2023Quote: ... 24 hours prior to induction the cells were cultured in 1 mM indole-3-acetic acid (IAA; Sigma, I5148), to ensure protein degradation ...
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bioRxiv - Microbiology 2023Quote: ... A Hybond N+ membrane (GE lifesciences, 45000854) and 6 sheets of 3 mm Whatman cellulose paper (Sigma Aldrich, WHA3030861) were pre- soaked for 5 minutes in room temperature 0.5X TBE ...
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bioRxiv - Immunology 2023Quote: Bone marrow supernatants (0.5 mL per sample) were concentrated with 3 kDa MWCO Amicon Ultra Centrifugal filter devices (Merck Millipore) up to a final volume of 30 μL ...
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bioRxiv - Cell Biology 2023Quote: ... Treatment of mice were performed with a single intraperitoneal injection of either vehicle (corn-oil) or 3 mg/kg 1,4-bis-[2-(3,5,-dichloropyridyloxy)] benzene (TCPOBOP, T1443, Sigma-Aldrich).
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bioRxiv - Cancer Biology 2023Quote: Culture medium was replaced with 100 µL medium containing 5 mg/mL filtered 3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) (#M5655, Sigma-Aldrich) and incubated for 2 h at 37°C ...
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Linear ubiquitination at damaged lysosomes induces local NF-κB activation and controls cell survivalbioRxiv - Cell Biology 2023Quote: ... Target cells were transduced with 0.5 mL of viral supernatant in 3 mL of total medium supplemented with 8 µg/mL polybrene (Merck Sigma). 2 to 4 days post transduction ...
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bioRxiv - Developmental Biology 2023Quote: ... Now the samples were permeabilized and blocked by incubating with 3 % donkey serum (Catalog no. D9663-10ML, Sigma Aldrich) and 2 % TritonX (Catalog no ...
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bioRxiv - Biophysics 2023Quote: ... Samples were concentrated to an A260ml−1 of 3 (100 nM) using an Amicon Ultra 0.5 ml spin column with a 100 kDa cutoff (Merck Millipore) to an OD260 absorbance of 5.0.
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bioRxiv - Biophysics 2023Quote: ... the fluorescence spectra of mRaichu were measured in solutions containing 3 mg/mL BSA (SIGMA, Product No. 017-25771), 100 mM NaCl ...
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bioRxiv - Biophysics 2023Quote: ... Samples were measured in a 3 mm path-length cuvette (Hellma, 105.251-QS) in PBS (Sigma-Aldrich, D8537-500ML) containing 500 mM NaCl adjusted at pH 7.4.
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bioRxiv - Plant Biology 2023Quote: ... 100 mg glass wool were filled with 200 µl (Z)-3-hexenyl acetate (HAC; >98%; Sigma-Aldrich, Buchs, Switzerland) diluted 50 fold in EtOH and sealed with screw caps containing a rubber septum ...
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bioRxiv - Immunology 2023Quote: ... and secondary antibodies were diluted in IF buffer and incubated for 1 hour at RT and washed 3 more times with PBS 0.05% Tween20 (Sigma). Sections were incubated with PBS DAPI (5 µg/mL ...
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bioRxiv - Developmental Biology 2024Quote: ... The embryos were rinsed and permeabilized in PBS-0.1% triton, 3 times 30 min, and incubated in blocking solution (PBS-0.1% triton, 1% donkey serum (D9663, Sigma)) prior to incubating with primary and secondary antibodies ...
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bioRxiv - Systems Biology 2024Quote: ... UK) previously coated with calf- skin collagen (15 µg/cm2 and fibronectin 3 µg/cm2; both Sigma-Aldrich, UK), were transfected with one of three different commercial siRNA sequences designed to target the EGFR or an Allstars negative control siRNA sequence (final concentration 1 nM ...
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bioRxiv - Systems Biology 2024Quote: ... UK) previously coated with calf- skin collagen (15 µg/cm2 and fibronectin 3 µg/cm2; both Sigma-Aldrich, UK). The permeability of hCMEC/D3 cell monolayers to 70 kDa FITC-dextran (2 mg/ml ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... media was aspirated from cells and replaced with 5 mg/mL 3-(4,5-Dimethyl-2-thiazolyl)-2,5-diphenyl-2H-tetrazolium bromide (MTT, #M5655, Sigma Aldrich) solution in base media ...
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bioRxiv - Neuroscience 2024Quote: ... paraformaldehyde in PBS and incubated for 30 min in blocking buffer containing 0.3% Triton X100 and 3% BSA (Sigma) in PBS to permeabilize the cells and block nonspecific staining ...
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bioRxiv - Molecular Biology 2024Quote: ... Cells were seeded at 1.0E5 cells/ml with 10 ng/ml IL-3 and 8μg/ml polybrene (Sigma-Aldrich). Virus was added to wells at 0 ...
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bioRxiv - Molecular Biology 2024Quote: ... Samples were treated with RNase A (345 μL TE, 3 μL 10 mg/mL RNAse A (Sigma-Aldrich, R6513), 2 μL 20 mg/mL Glycogen (Roche ...
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bioRxiv - Cancer Biology 2024Quote: ... The fixed and permeabilized cells were then exposed to a blocking solution (3% bovine serum albumin (#A7906, Sigma‒Aldrich) in PBS ...
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bioRxiv - Neuroscience 2024Quote: ... Free-floating organotypic slices or vibratome sections were incubated in permeabilization solution (0.2% Triton-X100, 3% BSA in PBS; Sigma) for 1 hour at room temperature ...
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bioRxiv - Physiology 2024Quote: ... The PDMS scaffold surface was activated oxygen plasma and then treated with a 5% (3-aminopropyl) triethoxysilane (Sigma-Aldrich) solution in methanol for 45 min ...
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Angiogenesis in the mature mouse cortex is governed in a region specific and Notch1 dependent mannerbioRxiv - Neuroscience 2024Quote: ... mice were intravenously injected with FITC-dextran (100µL of 3-5% w/v in 0.9% saline; 70kDa, Sigma #46945) or Texas Red dextran (100µL of 3% w/v in 0.9% saline ...
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bioRxiv - Neuroscience 2024Quote: Stock solutions (1mg/ml) of 1,1′-dioctadecyl-3,3,3′,3′-tetramethylindocarbocyanine perchlorate (DiI) in N,N-dimethylformamide (Sigma-Aldrich 468495) were stored at −20°C ...
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bioRxiv - Biophysics 2024Quote: ... Excised tissues are first allowed to fold into spheroids and seal in HM for 3-6 hours and are then embedded in 0.5% low gelling point agarose (Sigma) prepared in HM to reduce tissue movement during imaging ...
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bioRxiv - Cancer Biology 2024Quote: ... washed with PBS (3 times x 5 minutes) and incubated with secondary antibodies and Hoescht 33342 (Sigma-Aldrich, #14533) diluted in 1x Antibody diluent/Block solution for 2 hours at room temperature ...
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bioRxiv - Cell Biology 2024Quote: ... All the ASO probes were synthesized with biotin-TEG added to the 3’ end of the sequences (Sigma-Aldrich). Briefly ...
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bioRxiv - Cancer Biology 2024Quote: ... We used 3 and 5wt% GelMA with a 1:16 mass ratio of photoiniator (Lithium phenyl-2,4,6-trimethylbenzoylphosphinate, LAP; Sigma Aldrich). LAP and GelMA were dissolved together in Dulbecco’s Phosphate Buffered Saline (DPBS ...