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Citations for Millipore Sigma :
8701 - 8750 of 10000+ citations for Human Urocortin 3 UCN3 CLIA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2021Quote: ... glucose were pelleted by centrifugation and resuspended in 5 mL YNBNAG11 pH 5.1 containing 5 μM 3-MB-PP1 (EMD MILLIPORE) or DMSO as a vehicle control ...
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bioRxiv - Neuroscience 2020Quote: ... The sections were first washed 3×10min with PBS and then permeabilized with 0.1% Triton X-100 (T9284, Sigma-Aldrich) in PBS for 10 min at room temperature (RT) ...
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bioRxiv - Microbiology 2020Quote: ... CTXφ production was induced in these strains by growing up to OD600=0.3 followed by 16 hour incubation at 37°C with aeration in LB supplemented with mitomycin C (20ng/mL) (Sigma). V ...
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bioRxiv - Microbiology 2020Quote: ... Immunoprecipitation was performed at 4°C overnight with nutation in 1 ml chromatin aliquots with 3 ul of anti-FLAG M2 antibody (F3165, Sigma) and 20 ul of Protein G Dynabeads (Invitrogen) ...
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bioRxiv - Molecular Biology 2021Quote: ... media (ratio 1:3) for 30-45 min until the digestion was stopped by the addition of FBS (Sigma, Germany) at a 10 % v/v ratio ...
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Identification and biochemical characterization of a novel eukaryotic-like Ser/Thr kinase in E. colibioRxiv - Microbiology 2020Quote: ... coli DH5α cells were transformed with the resulting pho-lac fusion plasmids and streaked on dual indicator plates containing LB agar with 5-bromo-4-chloro-3-indolyl phosphate disodium salt (X-Phos) (Sigma) at a final concentration of 80μg/ml and 6-chloro-3-indolyl-β-D-galactoside (Red-Gal ...
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bioRxiv - Microbiology 2019Quote: ... The cells were washed 3 times with PBS containing 0.1% Tween-20 (Sigma Aldrich, Castle Hill, NSW, Australia; PBS-T) before being stained with 400μl AlexaFluor 488-labelled anti-Rabbit IgG (Thermo Fisher Scientific ...
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bioRxiv - Biochemistry 2021Quote: ... The purified proteins were analyzed by SDS-PAGE and MALDI-TOF and concentrated using ultra-filtration through a 3 kDa cut-off Amicon (Millipore) membrane ...
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bioRxiv - Neuroscience 2020Quote: ... animals were mounted by placing them in 3% agarose pads on glass slides and immobilized with 1 mM tetramisole hydrochloride (Sigma). Imaging was performed using Confocal Laser Scanning Confocal microscope (Olympus 1X61 ...
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bioRxiv - Cancer Biology 2020Quote: ... Percentage of GFP positive cell population was measured at day 3 or day 4 as initial time point using a Guava Easycyte HT instrument (Millipore). GFP% (mCherry% ...
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bioRxiv - Cell Biology 2021Quote: ... The reacted mixture was dialyzed in PBST buffer (DPBS with 0.05% v/v Tween 20) by a centrifugal filter with a 3 KDa molecular weight cut-off (Millipore UFC500324). The supernatant was immunoprecipitated by custom-made anti-RFP-beads (see Immunoprecipitation [IP] and Co-IP below) ...
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bioRxiv - Cell Biology 2021Quote: ... Lysates were then solubilized by mixing 3:1 lysate to Li-Cor 4x loading buffer (928-40004) plus 10% Beta-Mercaptoethanol (Sigma). Samples were denatured by boiling for 5 min followed by cooling on ice for 2 min ...
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bioRxiv - Cell Biology 2020Quote: ... followed by blocking buffer composed of PBS supplemented with 3% heat inactivated donkey serum (017-000-121, Jackson Immuno Research) and 0.1% Triton X-100 (T8787, Sigma-Aldrich). Primary antibodies were then diluted in blocking buffer and incubated overnight at 4°C ...
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bioRxiv - Cell Biology 2020Quote: ... were seeded in DMEM supplemented with 10 % FBS in 96 well plates incubated with increased concentrations of compound 2 and treated with 3-NP at 10 mM (#N5636, Sigma). Then ...
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bioRxiv - Bioengineering 2020Quote: ... Cerebral cortices of three pups were dissected under sterile conditions and kept on ice in a sterile Falcon tube (15 mL) containing 3 mL of trypsin-EDTA 0.25% (Sigma-Aldrich) for 10 min ...
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bioRxiv - Biophysics 2020Quote: ... the base was suspended in PBS-glucose (3 ml) and 1.5 ml were gently transfer on a Percoll gradient (Percoll 60%-Percoll 30%, Sigma Aldrich) and centrifuged 20 min at 1700 rpm ...
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bioRxiv - Biophysics 2021Quote: All proteins were concentrated using ultrafiltration concentrators with either 3 kDa or 30 kDa MWCO regenerated cellulose membrane (EMD Millipore), flash frozen in small aliquots in liquid nitrogen ...
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bioRxiv - Biophysics 2020Quote: ... As oxygen scavenger a combination of 1 mM Trolox and the P2O system (3 units per ml pyranose oxidase, 90 units per ml catalase and 50 mM glucose, Sigma) were used.
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bioRxiv - Cancer Biology 2021Quote: ... cells were treated with 0.25μM testosterone 3-(O-carboxymethyl) oxime:BSA-fluorescein isothiocyanate (T-BSA-FITC) conjugate (T5771, Sigma-Aldrich, Munich, Germany) diluted in Tris-Buffer (pH 7.2 ...
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bioRxiv - Cancer Biology 2021Quote: ... A coated microplate was pre-warmed at 37°C and 3×105 cells were seeded in 675 μL of bicarbonate-free Dulbecco’s Modified Eagle’s Medium (DMEM) (Sigma-Aldrich, D5030) supplemented with 1 mM pyruvate ...
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bioRxiv - Cell Biology 2021Quote: ... Neurons were plated at a density of ∼3 × 105 cells on 12-mm glass coverslips coated with 0.01% poly-L-lysine (Sigma, P4832). Half of the medium was changed every 3-4 days and experiments were performed at 12–13 days in vitro.
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bioRxiv - Cell Biology 2020Quote: ... cells were replica-plated onto pABA− (2% glucose, w/v) plates with 0 µM or 25 µM linolenic acid (C18:3, Sigma). Colonies that grew on 25 µM linolenic acid were picked into YEPD overnight cultures and struck on YEPD plates ...
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bioRxiv - Cell Biology 2020Quote: ... 0.5 or 1 or 2 mM, Sigma, P4543; carbamazepine, 5 or 50 or 100 μM, Sigma, C4024; phenytoin, 3 or 30 or 100 μM, Sigma, D4007 ...
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bioRxiv - Cell Biology 2020Quote: ... Western blotting was performed on 12% SDS-PAGE gels using 1-hour semi-dry transfer with 3% bovine serum albumin (BSA, Sigma) in TBS + 0.5% Tween-20 as a blocking agent.
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bioRxiv - Cell Biology 2022Quote: ... and 3 mL volumes of culture were transferred per well to 6-well tissue culture-treated polystyrene plates (Sigma CLS3506) and incubated at 23°C for 48 hours ...
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bioRxiv - Biochemistry 2022Quote: ... cells were washed in 60% isopropanol and stained in an ORO solution (2:3 v/v H2O: isopropanol, containing 0.5% ORO, Sigma O0625) for 1 hour ...
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bioRxiv - Biophysics 2022Quote: ... The surface was regenerated with two pulses (30 s at 100 µL/min) of Isopropanol:50 mM NaOH (2:3) solution followed by a 20 mM CHAPS (Sigma) or 40mM Octyl-beta-Glucoside (Sigma ...
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bioRxiv - Neuroscience 2022Quote: ... Cells were then blocked and permeabilized for 30 min at RT in blocking buffer that contained 3% bovine serum albumin (BSA) and 0.1% Triton X-100 (Sigma Aldrich). Subsequently ...
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bioRxiv - Cell Biology 2022Quote: ... Liposomes consisting of DPPC/egg yolk PC/egg yolk PG/cholesterol (6:3:1.2:0.95, DPPC from Avanti Polar Lipids, all other Sigma) with 0.6 μCi tracer [2-palmitoyl-1-14C]-dipalmitoyl phosphatidylcholine (14C-DPPC ...
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bioRxiv - Cell Biology 2022Quote: ... Cells were transduced with either a non-targeting control sgRNA or Zfp266 sgRNA lentivirus at an MOI of 3 with 8 µg ml−1 Polybrene (Merck-Millipore) for 4 hours ...
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bioRxiv - Cell Biology 2022Quote: ... 1 × 105 Cas9 TNG MKOS MEFs were transduced with either a non-targeting control sgRNA or Zfp266 sgRNA lentivirus at an MOI of 3 with 8 µg ml−1 polybrene (Merck-Millipore) for 4 hours ...
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bioRxiv - Biochemistry 2022Quote: ... The refolded solution was left static over one day at 4 °C and concentrated with a compressed nitrogen-gas (or air) driven ultrafiltration device (350 ml Amicon Stirred Cell, 3 kDa NMWL membrane, Millipore), during which time cysteine in the solution gradually react to form cystine and precipitated out and could usually clog the ultrafiltration membrane at the end phase of ultrafiltration ...
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bioRxiv - Biochemistry 2022Quote: ... were combined and concentrated using an Amicon concentrator tube (30 kDa MWCO for the Nb- fused biosensors, 3 kDa MWCO for HER2-Nb) (Merck-Millipore). A final volume < 5 mL was loaded onto a SEC column (HiLoad 200pg ...
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bioRxiv - Cell Biology 2022Quote: ... plasmids following a similar protocol and transgenic parasites were obtained after 3 weeks of selection with 100 μg/ml G418 and 200 μg/ml Hygromycin B (Sigma).
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bioRxiv - Biochemistry 2022Quote: ... coli strain MG1655-seeded NGM plates in the presence or absence of 1 mM auxin (IAA, indole-3-acetic acid, Sigma).
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bioRxiv - Cancer Biology 2022Quote: ... HD-MBO3 group 3 MB cells were generously provided by Till Milde (DKFZ, Germany) and cultured in RPMI medium (Sigma). Human embryonic kidney HEK-293T cells were cultured in Dulbecco’s modified Eagle’s medium (DMEM ...
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bioRxiv - Molecular Biology 2022Quote: ... Fractions containing pure Pae87 were desalted and concentrated with Amicon Ultra centrifugal filters of 3 kDa or 10 kDa MWCO (Millipore). Concentrated samples were centrifuged at 15,000 × g for 10 min prior to crystallization trials ...
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bioRxiv - Immunology 2022Quote: ... 3 mM 2-mercaptoethanol and the mixture was concentrated to 10 mg/ml (Protein concentration) using an Amicon Ultra-4 filter (3 kDa molecular-weight cutoff, Millipore). The FoxP3ΔN’–DNA complex was then mixed with the reservoir solution (0.1 M Tris-Hcl pH 8.5 ...
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bioRxiv - Physiology 2022Quote: ... were mixed with 3 µl proteinase K (20 mg/ml in 25 mM Tris-HCl, 5 mM Ca2Cl, pH 8.0, Sigma-Aldrich). This mixture was applied to the ear clips ...
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bioRxiv - Plant Biology 2022Quote: ... and sterilized for 3 h in a mixture of bleach (30 mL) and concentrated hydrochloric acid (1.5 mL, Sigma-Aldrich). The pgb1-1 mutant lacks ethylene-induced PGB1 expression and lower hypoxia induced PGB1 expression (relative to wild-type ...
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bioRxiv - Pathology 2022Quote: Lung tissue (4–20 g) was inflated with warm low temperature gelling agarose (3% by weight in DMEM w/o Phenol red, A9414, Sigma) at room temperature using 1-mL syringe ...
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bioRxiv - Microbiology 2022Quote: ... DSF (CAS No. 677354-23-3, purity >90.0%) and BDSF (CAS No. 55928-65-9, purity >95.0%) purchased from Sigma Aldrich (USA) and trans-BDSF (CAS No ...
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bioRxiv - Microbiology 2022Quote: ... Samples were centrifuged 1800 rpm for 6 minutes at 4°C and pellets were resuspended n 3 mL RBC lysis for 4 minutes (Sigma). Samples were centrifuged 1800 rpm for 6 minutes at 4°C and the pellets were resuspended in 200 μl of PBS + 2% FBS + 2 mM EDTA ...
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bioRxiv - Developmental Biology 2022Quote: ... and then stained for 2 minutes at room temperature using filtered 0.5% Solvent Black 3 (CAS Number 4197-25-5; Sigma 199664) dissolved in 75% ethanol ...
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bioRxiv - Developmental Biology 2022Quote: ... permeabilized for 30 min at room temperature in DPBS/0.3% Triton-X100 and block by blocking buffer (PBST containing 3% donkey serum (Sigma, D9663) and 1% BSA (Sigma ...
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bioRxiv - Genomics 2022Quote: ... the mice were anesthetized again with 3% isoflurane and immediately administered 100 μl of 15 mM AkaLumine-HCL (Sigma Aldrich) for imaging ...
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bioRxiv - Microbiology 2022Quote: Mice were fasted for 4hrs before oral gavage with 200uL of fluorescein isothiocyanate (FITC)-dextran (3-5 kDa, Sigma-Aldrich) dissolved in sterile PBS (60mg/ml) ...
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bioRxiv - Cell Biology 2022Quote: ... mycelia from 3-day old liquid cultures were harvested by straining the culture through Miracloth (Millipore Sigma catalog number 475855). The mycelia were transferred to a 50 ml Falcon tube and washed twice in 0.7 M NaCl by pelleting mycelia at 8,000 rpm and 25°C for 5 minutes using a JA25.50 rotor and resuspending in sterile saline solution ...
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bioRxiv - Biophysics 2022Quote: ... 1-oleoyl-2-(12-biotinyl(aminododecanoyl))-sn-glycero-3-phosphoethanolamine (biotin-PE) and Tween-20 were purchased from Sigma Aldrich. Tween-20 was suspended in 50 mM Tris buffer (pH 8 ...
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bioRxiv - Cell Biology 2022Quote: ... Cell supernatant containing the virus was collected 3 days after transfection and 20-fold concentrated using Amicon Ultra 15 10K (Millipore) centrifugal filter ...