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Citations for Millipore Sigma :
8351 - 8400 of 10000+ citations for Human Urocortin 3 UCN3 CLIA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2023Quote: ... for 1 hour followed by 3 hours of stimulation using 50 nM phorbol myristate acetate (PMA, Sigma-Aldrich, P1585), 25 μg/ml Lipopolysaccharide (LPS ...
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bioRxiv - Genomics 2023Quote: ... The aqueous layer was separated using 50 μL 1-bromo-3-chloropropane (BCP; Sigma-Aldrich #B9673, Burlington, MA, USA) per 500 μL of Trizol and centrifuged at 12000 x g for 15 minutes at 4°C ...
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bioRxiv - Biophysics 2023Quote: ... NMR samples were buffer exchanged into NMR buffer (pH 7.4 or pH 7.8) using Amicon Ultra-15 centrifugal concentrators (3-kDa cutoff, Millipore Sigma) to a final nucleic acid concentration of ∼1 mM ...
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bioRxiv - Neuroscience 2023Quote: ... by dispensing individual cells in 5 nL drops directly into 3 µL ice-cold single cell lysis buffer (scLB, 0.134% Triton X-100 [Sigma] ...
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bioRxiv - Neuroscience 2023Quote: ... Slides were then washed in 80% ethanol for 2-3 minutes and differentiated in 0.1% lithium carbonate (Sigma #62470) for 45-60 seconds followed quickly by washes in distilled water to stop the differentiation ...
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bioRxiv - Immunology 2023Quote: Mouse sera were diluted 1:100 in an assay buffer of 0.05% PBS-T supplemented with 3% (w/v) BSA (Sigma) and transferred into a 96-well plate ...
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bioRxiv - Bioengineering 2023Quote: ... Cells were then permeabilized with 0.1% Triton-X for 10 min and blocked with 3% goat serum (GS) (Sigma) for 30 mins at room temperature (unless stated otherwise) ...
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bioRxiv - Biochemistry 2023Quote: ... The eluted protein was concentrated and using using Amicon Ultra-15 centrifugal filters (3 kDa cut-off, Merck-Millipore) and then buffer was exchanged to buffer B (50 mM Tris-HCl pH 8.0 ...
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bioRxiv - Biophysics 2023Quote: ... Peak fractions were concentrated to ∼ 3-4 mg mL-1 using a 100 kDa MWCO centrifugal concentrator (Millipore Sigma) for cryo-EM sample preparation.
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bioRxiv - Neuroscience 2023Quote: ... 3-5 organoids per condition were dissociated by incubation with a solution of 0,5 mg/ml Collagenase/Dispase (Sigma) + 0,22 mg/ml EDTA (Euroclone ...
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bioRxiv - Neuroscience 2023Quote: Cells in the tectum were transfected using in vivo electroporation of stage 46 animals anesthetized in 0.01%MS-222 (3-aminobenzoic acid ethyl ester, Sigma) dissolved in Steinberg’s media ...
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bioRxiv - Microbiology 2023Quote: ... 5 mg/ml of 3-(4,5-Dimethyl-2-thiazolyl)-2,5-diphenyltetrazolium bromide (MTT, Sigma-Aldrich, St. Louis, MI, USA) solution was added to the cells and incubated for an additional 4 h ...
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bioRxiv - Immunology 2023Quote: ... Tumors were digested for 45 minutes with gentle shaking at 37°C in HBSS containing 3 mg/ml Collagenase IV and 2.8 mg/ml DNase-I (Sigma). Cells from BRAFi (n = 5 ...
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bioRxiv - Microbiology 2023Quote: The effect of drug treatments on cell viability was assessed using the MTT [3-(4,5- dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide] assay (the MTT powder was obtained from Sigma Aldrich). VTN or Huh-7 cells were seeded the day before treatment at a concentration of 7L×L103 cells per well in DMEM supplemented with 10% FBS and 1% Pen/Strep ...
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bioRxiv - Immunology 2023Quote: ... and fixed for 3 hr at room temperature (RT) in freshly prepared Carnoy’s fixative [60% anhydrous methanol (Sigma-Aldrich), 30% chloroform (Sigma-Aldrich) ...
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bioRxiv - Microbiology 2023Quote: ... wells were incubated with 200 µL of a blocking buffer (PBS - Bovine Serum Albumin 3% (BSA reference: A9418, Sigma)) during one hour at 35°C ...
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bioRxiv - Cell Biology 2023Quote: The cell proliferation assay was determined by 3-(4, 5-dimethylthiazol-2-yl)-2,5-diphenyl-tetrazolium bromide (MTT) assay (Sigma). All cancer cell lines were seeded in 24-well plates (2×104 cells/well) ...
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bioRxiv - Bioengineering 2023Quote: ... or C43 cells in M9 minimal media supplemented with 0.5 g 15N NH4Cl2 (Cambridge isotopes) and/or 3 g 13C glucose (Sigma) per liter as well as 0.2 g/L of isotope- enriched ISOGROW supplements (Sigma) ...
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bioRxiv - Pathology 2023Quote: ... HUVECs were then exposed to shear stress for 24 hours with or without Tubastatin-A (3 µM; Sigma-SML0044). For experiments where autophagic flux was blocked ...
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bioRxiv - Bioengineering 2023Quote: ... the organosilane used was hexadecyltriethoxysilane (HDTES, Gelest Inc, cat no SIH5922.0) rather than (3-aminopropyl) trimethoxy silane (APTMS, Sigma).
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bioRxiv - Bioengineering 2023Quote: ... with the substitution of hexadecyltriethoxysilane (HDTES, Gelest Inc., cat no SIH5922.0) as the organosilane rather than (3-aminopropyl) trimethoxy silane (APTMS, Sigma).
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bioRxiv - Bioengineering 2022Quote: ... 3 mM aniline dimer solution was prepared by dissolving 5.5 mg of N-phenyl-p-phenylenediamine (Sigma-Aldrich, 241393) in 10 mL of Tyrode’s solution ~20 h at room temperature ...
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bioRxiv - Cancer Biology 2022Quote: ... Five thousand cNF-derived primary SCs from 3 independent patients (see Supplemental Table 2) were plated onto 0.1 mg/mL Poly-L-lysine (Sigma) and 4 μg/mL Laminin (Gibco)-coated opaque 96-well plates in SCM without IBMX with Forskolin and maintained at 37ºC under a 10% CO2 atmosphere for 24 hours ...
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bioRxiv - Cancer Biology 2022Quote: One hundred thousand primary SCs from 3 independent patients (see Supplemental Table 2) were plated onto 0.1 mg/mL Poly-L-lysine (Sigma) and 4 μg/mL Laminin (Gibco)-coated 12-well plates ...
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bioRxiv - Cancer Biology 2022Quote: ... One hundred thousand primary SCs from 3 independent patients (see Supplemental Table 2) were plated onto 0.1 mg/mL Poly-L-lysine (Sigma) and 4 μg/mL Laminin (Gibco)-coated 12-well plates in SCM without IBMX ...
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bioRxiv - Cancer Biology 2022Quote: Cells were collected by trypsinisation and resuspended in Phosphate-buffered saline and incubated with 200μM cyanide 3-chlorophenylhydrazone (CCCP, Sigma) for 15 min as TMRE negative control ...
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bioRxiv - Biophysics 2022Quote: ... Concentrated Cav1.2(ΔC)/Cavβ3/Cavα2δ-1 sample was immediately incubated with gabapentin (final concentration 2 mg ml-1, 11.7 mM) (Sigma-Aldrich) on ice for four hours prior to cryo-EM sample preparation ...
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bioRxiv - Genetics 2023Quote: ... 10-200 mg tissue was digested in 1 ml 3 N hydrochloric acid (Fisher)/10% trichloroacetic acid (Millipore Sigma) at 65 °C for two days ...
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bioRxiv - Developmental Biology 2023Quote: ... unlysed embryos were cultured during 2 to 3 hours in 40 µL micro drops of TCM199 (Sigma-Aldrich, M4530) + 10% foetal bovine serum (FBS ...
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bioRxiv - Immunology 2023Quote: ... juvenile or adult zebrafish were added to water containing 0.08 mg/mL Tricaine (MS222/ethyl 3-aminobenzoate; Sigma-Aldrich) until fish became non-responsive to touch (<5 minutes) ...
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bioRxiv - Cell Biology 2023Quote: ... Sections of 3 μm in the thickness were conducted for Safranin O and fast green and TRAP (Sigma-Aldrich) staining ...
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bioRxiv - Biophysics 2023Quote: ... Unbound dye was removed by sequential buffer exchange in centrifugal filters Amicon Ultra 0.5 ml 3 K (Merk Millipore). Lge11-80-Dylight labelled protein was stored at -80 °C.
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bioRxiv - Neuroscience 2023Quote: ... USA). 3- sulfopropionic acid (cat. #BD01044758) was purchased from BLD pharma. Tramiprosate (cat. # A76109) was purchased from Sigma Aldrich. GM1 and GM3 ...
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bioRxiv - Neuroscience 2023Quote: ... The cell viability was measured by quantifying the activity of cellular dehydrogenases that reduce MTT (3-4,5-dimethyl thiazolyl-2,5-diphenyl-2H-tetrazolium bromide, Sigma Inc.) to a purple formazan salt [35] ...
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bioRxiv - Neuroscience 2023Quote: 17β-Estradiol (E2, E8875), β-Estradiol-3-Benzoate (EB, E8515) and Progesterone (P, P0130) were purchased from Sigma-Aldrich and dissolved in sesame oil ...
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bioRxiv - Bioengineering 2023Quote: ... Nuclei were stained at room temperature for 3 minutes with DAPI (Sigma Aldrich, D9542, 1:500, 1 mg/ml). All samples were washed with PBS and covered with aluminium foil until imaging.
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bioRxiv - Bioengineering 2023Quote: ... Nuclei were stained at room temperature for 3 minutes with DAPI (Sigma Aldrich, D9542, 1:500, 1 mg/ml).
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bioRxiv - Evolutionary Biology 2023Quote: ... medaka and zebrafish were deeply anesthetized with 0.02% ethyl 3-aminobenzoate methanesulfonate (MS-222) (Sigma-Aldrich, St. Louis, MO) and fixed by perfusion with 4% paraformaldehyde (PFA ...
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bioRxiv - Microbiology 2023Quote: ... The supernatant was transferred to an Amicon Ultra-0.5 Centrifugal Filter Unit 3 kDa (Merck Millipore, Catalogue no. UFC500396) and centrifuged at 10,000 g for 20 min at 4°C ...
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bioRxiv - Bioengineering 2023Quote: ... KTB21 human mammary basal epithelial cell line was cultured in epithelial cell growth medium (DMEM [low glucose]: Ham’s F12 [1:3] medium supplemented with 5% FBS [Thermo Fisher Scientific], 0.4 μL/mL hydrocortisone [Sigma] ...
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bioRxiv - Developmental Biology 2023Quote: ... Fixed embryos were washed 3 times (5 minutes each) in wash buffer DPBS Tween containing 2% BSA (Sigma, A3311), and permeabilised at room temperature for 20 minutes in permeabilisation buffer 0.5% Triton-X in DPBS ...
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bioRxiv - Cancer Biology 2023Quote: ... 0.5mg/ml of 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) (Sigma-Aldrich®, St Louis, MO, USA) was added and incubated at 37°C for 3h ...
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bioRxiv - Cancer Biology 2023Quote: ... Membranes were again washed 3 × 5 min with PBS + 0.1% Tween 20 and incubated with Classico/Crescendo Western HRP substrate (Millipore-Sigma) for 2 min ...
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bioRxiv - Microbiology 2023Quote: ... These lysates were then additionally treated for protein precipitation with 3 volumes of ultra-pure methanol (Sigma-Aldrich, USA). Ultimately ...
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bioRxiv - Plant Biology 2023Quote: ... spy-3 and spy-4 were sprayed with either 50 µM or 100 µM BA (6-benzylaminoadenine, Sigma B3408) or mock [200 mM NaOH] three times ...
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bioRxiv - Microbiology 2023Quote: ... The membrane was probed with an α-FLAG antibody (Sigma; 1:1,000 in 1x TBS-T containing 3% BSA) at 4 °C overnight ...
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bioRxiv - Microbiology 2023Quote: ... For the serial passaging MDCK cell were infected at a multiplicity of infection (MOI) of 0.0001 and incubated for 3 days at 37°C in DMEM containing TPCK-Trypsin (Sigma) at a final concentration of 1 µg/mL ...
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bioRxiv - Microbiology 2023Quote: ... Cell viability was determined by the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) to MTT formazan conversion assay (Sigma-Aldrich), as described (Pizzato Scomazzon et al. ...
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bioRxiv - Microbiology 2023Quote: ... Wells were washed with sterile PBS x 3 and cells were lysed with 0.25 mL of 1% (weight/volume) saponin (Sigma) for 10 minutes ...
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bioRxiv - Microbiology 2023Quote: ... Calu-3 cells (ATCC, HTB-55) were maintained in Eagle’s minimum essential medium (EMEM) (Sigma-Aldrich, Cat# M4655-500ML) containing 10% FBS and 1% PS ...