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Citations for Millipore Sigma :
8801 - 8850 of 10000+ citations for Human Urocortin 3 UCN3 CLIA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2022Quote: ... The gelatin was subsequently cross-linked using N-(3-Dimethylaminopropyl)-N’-ethylcarbodiimide hydrochloride (EDC hydrochloride) (Sigma Aldrich Catalog No-03450) and N-Hydroxysuccinimide (NHS ...
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bioRxiv - Developmental Biology 2019Quote: ... 1-octen-3-ol and acetophenone) larvae were treated with respective odorants of the highest available purity (>99% from Sigma) Acetic acid (Sigma 2722) ...
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bioRxiv - Plant Biology 2019Quote: ... and 10 µg (except for Fig S12 (3 µg)) was loaded on SDS-PAGE before transferring to PVDF membrane (Merck Millipore). SlMai1-myc or synSlMai1-myc proteins were detected using anti-Myc antibodies (GenScript ...
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bioRxiv - Immunology 2019Quote: ... Fluorescein isothiocyanate (FITC) [2-(6-hydroxy-3-oxo-3h-xanthen-9-yl)-5-isothiocyanatobenzoic acid] was purchased from Sigma-Aldrich Korea ...
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bioRxiv - Biochemistry 2019Quote: The custom designed CompoZr® Zinc Finger Nuclease Plasmid targeted to exon 3 of the mouse ECH1 gene on chromosome 7 was obtained from Sigma. In-vitro transcription of the ZFN mRNA was performed following the manufacturer’s instructions ...
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bioRxiv - Bioengineering 2020Quote: ... This solution was subsequently mixed with 2.5 μL of 280 mM OAc and 1 μL of positive control DNA or 50 nM negative control (CTX-M ESBL: 5’-ATTGACGTGC TTTTCCGCAA TCGGATTATA GTTAACAAGG TCAGATTTTT TGATCTCAAC TCGCTGATTT AACAGATTCG GTTCGCTTTC ACTTTTCTTC-3’; Sigma) to create the final RPA mix ...
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bioRxiv - Plant Biology 2019Quote: ... The total proteins were extracted by using lysis buffer and then incubated for 3 h at 4°C with 20 ul of Anti-HA Affinity Gel (Millipore). The immunoprecipitates were washed five times with lysis buffer ...
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bioRxiv - Molecular Biology 2019Quote: ... Small RNAs were crosslinked to the membrane by chemical crosslink using N-(3-Dimethylaminopropyl)-N’-ethylcarbodiimide hydrochloride (EDC, Sigma-Aldrich). Membrane was pre-hybridized for 20 min with PerfectHyb(tm ...
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bioRxiv - Molecular Biology 2019Quote: ... cells were arrested in G1 as above and 1 h prior to release auxin (indole-3-acetic acid sodium salt; Sigma) was added to a final concentration of 1 mM ...
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bioRxiv - Cell Biology 2019Quote: ... cells were seeded at a density of ∼70,000 cells.ml−1 on 18 mm glass coverslips coated with poly-L-lysine (3 min application of 0.1 mM polylysine (Sigma P2636) in borate buffer ...
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bioRxiv - Cell Biology 2019Quote: ... Normalization of the data was performed using the set of phosphopeptide standards (added to the sample prior phosphoenrichment step; MS PhosphoMix 1, 2, 3 Light, Sigma) and by non-phosphorylated peptides identified in direct analyses.
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LIR-dependent LMX1A/LMX1B autophagy crosstalk shapes human midbrain dopaminergic neuronal resiliencebioRxiv - Cell Biology 2019Quote: ... and typically plated at 1:2-1:3 dilution ratio in neural differentiation media supplemented with RevitaCell on plates coated with poly-L-ornithine (Sigma) and laminin (1/1000 in PBS ...
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bioRxiv - Cell Biology 2019Quote: ... injected embryos were dechorionated and embedded in 2-mm or 3-mm glass capillaries (Hilgenberg GmbH) filled with 0.9% low melting temperature agarose (Type VII, Sigma Aldrich) prepared in zebrafish system water ...
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bioRxiv - Neuroscience 2019Quote: Astrocytes were plated on PO/Lam coated 96 well plates and treated with 3 ng/mL IL-1α (Sigma; 3901), 30 ng/mL tumor necrosis factor alpha (R&D Systems ...
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bioRxiv - Genetics 2019Quote: ... Lysates were diluted 3-fold in 20 mM HEPES pH 8.0 and digested overnight in 10 μg/mL trypsin-TPCK (Sigma-Aldrich) and 10 μM HCl ...
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bioRxiv - Neuroscience 2019Quote: ... the samples containing the purified CNTNAP2-ecto and the vehicle control were dialyzed 3 times using 100 kDa spin filters (Millipore) to remove the glycine and substitute it with a TBS solution.
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bioRxiv - Microbiology 2019Quote: ... The cells were co-cultured for 3 days in FBS-free medium supplemented with trypsin (0.5 μg/mL) (Sigma Aldrich). After incubation ...
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bioRxiv - Bioengineering 2019Quote: ... each of which had a 3 m long polar SUPELCOWAX® 10 column (250 µm x 0.25 µm, Sigma Aldrich). Note that while polar columns have been used in the 2nd-dimensional column in 2D GC analysis of breath28 ...
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bioRxiv - Pathology 2019Quote: ... all drug treatments (1 μM Cysteamine; Sigma, 100 nM Everolimus; RAD001 Selleckchem, 30 mM 3-methyladenine; Sigma, 50 mM Sucrose; Sigma) were added to cell culture medium and incubated with the cells for 24 hrs.
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bioRxiv - Molecular Biology 2019Quote: ... degradation of V5-AID-fused Brn1 protein was induced by addition of 0.5 mM indole-3-acetic acid (Auxin, Sigma # 13750).
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bioRxiv - Physiology 2019Quote: ... Fixed tissues were blocked and permeabilized in calcium and magnesium free PBS supplemented with 3% BSA and 0.2% Triton X100 (both obtained from Sigma Aldrich) for 1 h at RT ...
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bioRxiv - Developmental Biology 2019Quote: ... the supernatant was transferred into a new tube (and the DNA concentration determined by NanoDrop 2000 Spectrophotometer .25 μg of chromatin were used to immunoprecipitate with 3 μg of the rabbit anti H3K27me3 antibody (Millipore) previously bound to dynabeads overnight at 4°C ...
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bioRxiv - Developmental Biology 2019Quote: ... animals were anesthetized by bath application of 0.02% w/v solution of Ethyl-3-aminobenzoate methanesulfonate (Sigma-Aldrich, St. Louis) in filtered fish system water for 1 min ...
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bioRxiv - Cell Biology 2019Quote: ... Bcl2/Adenovirus E1B 19 kDa and protein-interacting protein 3-like (BNIP3L/NIX) from Sigma-Aldrich (St. Louis, MO, USA), perilipin (PLIN ...
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bioRxiv - Neuroscience 2019Quote: ... the sections were washed (3 × 5 min) in 0.1 M PBS and incubated with goat anti-rabbit Alexa488 (1:500; Sigma-Aldrich) secondary antibody (to label collagen-I ...
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bioRxiv - Pharmacology and Toxicology 2019Quote: ... Protein samples from 24 mice of both strains (n = 3 per group) were separated by SDS-PAGE (8-20%) and transferred onto PVDF membranes (Millipore). Afterward ...
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bioRxiv - Plant Biology 2019Quote: ... was assessed by evaluating the cell viability by MTT assay using 3-(4,5-dim-ethylthiazol-2-yl)-2,5-diphenyl-tetrazolium bromide reagent or MTT (Sigma-Aldrich®) [36] ...
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bioRxiv - Microbiology 2019Quote: ... 3% bovine serum albumin was using to block samples before staining with primary (α-myc Alexa Fluor 488 conjugated (Millipore), 1:500 ...
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bioRxiv - Cancer Biology 2020Quote: ... 3 μg proteins were separated by SDS-PAGE and electrotransferred onto Immobilon®-P transfer membranes (Merck Millipore, MA, USA), blocked with 5% skim milk in Tris-buffered saline with 5% Tween 20 (TBST) ...
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bioRxiv - Developmental Biology 2020Quote: ... cells were exposed to 5 μM CHIR99021 (Axon) between days 2 and 3 and then supplemented with 100 nM RA (Sigma) until day 5 ...
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bioRxiv - Synthetic Biology 2020Quote: ... Single colonies were grown overnight at 37 °C in 3 ml of LB (Hy-Labs) supplemented with 100 μg/ml ampicillin (Sigma). Plasmid DNA was purified using QIAprep spin miniprep kit (Qiagen ...
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bioRxiv - Molecular Biology 2019Quote: ... Color was developed with freshly prepared 5-bromo-4-chloro-3-indolyl phosphate/nitro blue tetrazolium (BCIP/NBT; Sigma-Aldrich) and the reaction was stopped by addition of 2 mM ethylenediaminetetraacetic acid (EDTA).
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bioRxiv - Cell Biology 2019Quote: ... Six μm sections of formalin-fixed paraffin-embedded tissue from the cortex frontal (n=3) were used to stain NeuN (Millipore), IBA1 (Wako) ...
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bioRxiv - Cell Biology 2020Quote: ... Progeny were collected at eclosion and allowed to mate and develop for 3-5 days before being transferred to food mixed with 50ug/mL mifepristone (RU486, Sigma) or ethanol (control ...
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bioRxiv - Developmental Biology 2019Quote: ... Dechorionated live embryos were incubated for 6, 12 and 24 hours in 40μM Nifedipine or 20mM BDM (2, 3-butanedione-2-monoxime) (Sigma-Aldrich) in Danieau’s solution ...
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bioRxiv - Molecular Biology 2020Quote: 25 mm round cover glass (Fisher Brand, #12-545-102 25CIR-1) was coated with 3-Triethoxysilypropylamine APES (Millipore, #A3648) following manufacturer’s instructions ...
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bioRxiv - Physiology 2019Quote: ... heat-polished patch pipettes with tip resistances between 3 and 5 MΩ were used and contained amphotericin B (Sigma-Aldrich) as the perforating agent ...
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bioRxiv - Cell Biology 2019Quote: ... Normalization of the data was performed using a set of phosphopeptide standards (added to the sample prior phosphoenrichment step; MS PhosphoMix 1, 2, 3 Light, Sigma) in combination with summed peak area of non-phosphorylated peptides identified in analyses of samples without phosphoenrichment ...
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bioRxiv - Developmental Biology 2020Quote: ... membranes were washed 3 times with TBS-T and subjected to chemiluminescence detection with Immobilon Western Chemiluminescent HRP (Horseradish Peroxidase) Substrate (Merck Millipore) using a Gel-Doc XR+ System (BioRad) ...
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bioRxiv - Microbiology 2020Quote: ... and the resulting adhered bacteria on the cover slip were stained with 200 μL of the membrane stain N-(3-Triethylammoniumpropyl)-4-(6-(4-(Diethylamino) Phenyl) Hexatrienyl) Pyridinium Dibromide (FM4-64) (Sigma) at a final concentration of 20 μg/mL for 5 minutes ...
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bioRxiv - Cell Biology 2019Quote: ... and then washed twice with PBS following incubation in blocking solution containing 10% goat serum or 3% Bovine Serum Albumin (Sigma) and 0.1% Triton X-100 (Sigma ...
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bioRxiv - Cell Biology 2019Quote: ... supplemented with Small molecule inhibitors PD0325901 (PD, 1 μM, Selleck) and CHIRON99021 (CH, 3 μM, Selleck) and 1000 U/ml LIF (ESGRO, Millipore). Cells were routinely propagated on 0.1% gelatin-coated plastic and replated every 3 days at a split ratio of 1 in 10 following dissociation with Accutase (Gibco ...
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bioRxiv - Immunology 2020Quote: ... Peptides were separated from the HLA molecule remnants by ultrafiltration employing 3 kDa and 10 kDa Amicon filter units (Merck Millipore) for HLA-I and HLA-II ...
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bioRxiv - Genetics 2021Quote: In vitro treatments were carried out with different compounds: 1 μM MG-132 24 hours or 3 hours (Merck Millipore), 20 μM Chloroquine (CQ ...
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bioRxiv - Biochemistry 2020Quote: The pure protein recovered after HPLC purification in 1% acetic acid was concentrated in Amicon Ultra-15 (cut-off 3 kDa) centrifugal filter units (Merck Millipore) to reach the final protein concentration of 1 mM ...
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bioRxiv - Neuroscience 2020Quote: ... The pellet was resuspended in HB and loaded onto a three-step Percoll gradient (3%, 10%, and 23%; Sigma-Aldrich) in HB without protease inhibitor cocktail ...
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bioRxiv - Molecular Biology 2019Quote: ... Proteins were eluted using 500 μl of IP Buffer containing 0.25 mg/ml of the 3×Flag peptide (Sigma-Aldrich) and 1× cOmplete EDTA-free for 15 min at 4°C ...
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bioRxiv - Neuroscience 2020Quote: ... was injected into the heart chamber of the fish along with a high molecular weight fluorescent dye (2000 kDa dextran-conjugated FITC (3 mg/ml, Sigma). We estimate that 1 nl of a 10 nM Aβ injection into a ∼3.01 (±0.16 ...
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bioRxiv - Neuroscience 2020Quote: ... 3% Triton-X in PBS) for 1 h before incubation with primary antibodies (1:1000 anti-tyrosine hydroxylase, #AB152, Millipore UK ...
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bioRxiv - Genetics 2020Quote: ... 150mM NaCl) including 3% milk powder for 1 hour and incubated overnight with anti-FLAG antibody (Sigma Aldrich, cat. # F1804) in TBS plus 3% milk ...