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Citations for Millipore Sigma :
7801 - 7850 of 9891 citations for SUMO2 3 Blocking Peptide since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2022Quote: ... or 300 mM (octamer-mix + APLFAD) ammonium acetate at pH 7.5 using 3 kDa MWCO Amicon Ultra Centrifugal Filter Units (Merck Millipore). After buffer exchange the volume of each sample was ∼40 µL ...
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bioRxiv - Microbiology 2022Quote: ... 5 mg/ml of 3-(4,5-Dimethyl-2-thiazolyl)-2,5-diphenyltetrazolium bromide (MTT, Sigma-Aldrich, St. Louis, MI, USA) solution was applied to the cells and incubated for an additional 4h ...
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bioRxiv - Synthetic Biology 2022Quote: ... The silanization solution was freshly prepared by mixing MilliQ water with 0.4% (v/v) 3-(Trimethoxysilyl)propylmethacrylat (TMSPMA, Sigma) and 0.4% (v/v ...
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bioRxiv - Plant Biology 2022Quote: ... The transformants were then spotted on SD (−Trp, −Leu, −His) selection media containing 1 mM 3-amino-1,2,4-triazole (Sigma, A8056). The positive interactors were then scored based on the stringency of the selection.
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bioRxiv - Molecular Biology 2023Quote: ... mice were intraperitoneally injected with tamoxifen (0.08mg/g body weight per day for 3 days; Sigma-Aldrich, T5648-1G). For chemical-treated animals’ assay ...
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bioRxiv - Neuroscience 2023Quote: ... cells were harvested and lysed by 3 cycles of thawing and freezing followed by treatment with benzoase nuclease (Millipore). After pelleting down cell debris (16.9 g/rcf ...
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bioRxiv - Neuroscience 2023Quote: ... and then received 0.1mL of either 1.5-3% Fluorescein isothiocyanate–dextran (FITC) or Texas Red dextran (70kDa, Sigma-Aldrich #46945 and Thermofisher D1830 ...
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bioRxiv - Neuroscience 2022Quote: ... Fish were then anesthetized by applying 0.2 mg/mL ethyl-3-aminobenzoic acid ethyl ester (MESAB, Sigma-Aldrich E10521) to the fish mounted in agarose ...
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bioRxiv - Microbiology 2023Quote: ... the supernatant from late-log cultures was concentrated to <100 μL and washed (with 400 μL of ddH2O) in 0.5 mL centrifugal filter units (3-kDa molecular weight cutoff, Millipore), using centrifugation at 14,000 g ...
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bioRxiv - Neuroscience 2023Quote: ... From day 3 to day 15 1µM retinoic acid (SigmaAldrich, R2625) and 1µM Sonic Hedgehog Signaling Agonist (Millipore, 566660) were added ...
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bioRxiv - Plant Biology 2023Quote: ... above the tips with the help of forceps and placed into a 35-mm-diameter dish containing a 70 µm strainer and 3 ml enzyme solution (1.25% [w/v] Cellulase [“ONOZUKA” R10, Yakult], 0.1% [w/v] Pectolyase [P-3026, Sigma-Aldrich] ...
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bioRxiv - Systems Biology 2023Quote: Buffy Coat was diluted with PBS at a 1:3 dilution and underlayed with Histopaque®-1077 (Sigma-Aldrich), centrifuged at 350g for 30 minutes at 24°C with no brake ...
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bioRxiv - Microbiology 2022Quote: ... the bottom channel of a polydimethylsiloxane (PDMS) device was precoated with fibronectin (3 μg/ml, Sigma-Aldrich, Cat# F1141). The microfluidic device was generated according to our previous report70 ...
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bioRxiv - Neuroscience 2023Quote: ... 100 μg/mL streptomycin and 1 mM N6,2′-O-Dibutyryladenosine 3′,5′-cyclic sodium monophosphate salt (dbcAMP) (Sigma®). Cells were maintained with the differentiation medium at 37 °C in 5% CO2 for 3 days.
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bioRxiv - Physiology 2022Quote: ... cells were treated with the medium supplemented with 0.5 mM 3’-Isobutyl-1-methylxanthine (IBMX) (Sigma, Cat. I7018-1G), 1 µM dexamethasone (Sigma ...
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bioRxiv - Neuroscience 2022Quote: ... Passive dextran permeability was measured by culturing iBECs on Ø 0.4 μm pore polyester or polycarbonate Transwell inserts and cells were exposed to 0.5 mg/mL fluorescein isothiocyanate (FITC)-conjugated 3 – 5 kDa dextran (Sigma) for 24h ...
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bioRxiv - Cell Biology 2022Quote: ... 6- to 8-week-old mice of the indicated genotypes were intraperitoneally injected with 2.5 ml 3% thioglycollate (T9032, Sigma). On day 4 after the thioglycollate injection ...
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bioRxiv - Cell Biology 2022Quote: ... cells were washed 3 times with DPBS for 5 min and blocked in 1% BSA (Sigma-Aldrich, cat# A4503) in PBS-T (DPBS with 0.05% Tween 20 ...
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bioRxiv - Microbiology 2022Quote: ... 2 mL aliquots were taken from the main culture for chemical fixation with 3% paraformaldehyde (PFA, Sigma-Aldrich, #F8775) at 20 min at RT ...
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bioRxiv - Neuroscience 2022Quote: ... The PDMS microstructures were then rinsed with 2:3 HEPES1/ethanol and with ultrapure water (Milli-Q, Merck-MilliPore). PVP (1.3 MDa ...
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bioRxiv - Neuroscience 2022Quote: ... then selected after 24-48 hrs for 3 to 14 days with 8 µg/mL of puromycin (Sigma-Aldrich).
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bioRxiv - Plant Biology 2022Quote: Analytical reference standards were used for the following analytes: indole-3-acetic acid (IAA; Sigma-Aldrich St. Louis, MO), N-(3-indolylacetyl)-DL-aspartic acid (IAA-Asp ...
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bioRxiv - Developmental Biology 2022Quote: ... BP310-1], 10 mM NaCl [Fisher, Cat. M-11624], 3 mM MgCl2 [Sigma, Cat. M8226], 0.1% IGEPAL [v/v; Sigma, I8896] ...
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bioRxiv - Developmental Biology 2022Quote: ... 10 mM HEPES-KOH, pH 7.2 [Fisher, Cat. BP310-1], 10 mM NaCl [Fisher, Cat. M-11624], 3 mM MgCl2 [Sigma, Cat ...
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bioRxiv - Bioengineering 2022Quote: ... MSCs were frozen at passage 3 with 1.25*106 cells/ml in freezing medium containing FBS (BCBV7611, Sigma-Aldrich) with 10% dimethylsulfoxide (DMSO ...
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bioRxiv - Plant Biology 2024Quote: ... After immunolabeling the coverslips were incubated in Acryloyl-X-SE 0.1 mg/mL in PBS (ThermoFischer) overnight at RT and then in the monomer solution (11.7% NaCl, 3 % acrylamide (Sigma), 0.15% N,N′-methylenebisacrylamide (Sigma) ...
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bioRxiv - Neuroscience 2024Quote: ... microglia were treated with the respective concentrations of selected drugs (see Table 3) or DMSO (Sigma-Aldrich, Cat #:472301) as control and incubated for 6hrs and 24hrs before harvest for RNA extraction ...
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bioRxiv - Genetics 2024Quote: ... Zygotes with visible pronuclei washed 3 times in HTF medium and transferred to KSOM medium (Millipore, MR-106-D) and cultured in low oxygen chamber filled with the mixture gas (5% O2/ 5% CO2) ...
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bioRxiv - Plant Biology 2024Quote: ... They were then resuspended in 10 mM MES-KOH buffer (pH 5.7) with 10 mM MgCl2 and 100 mM acetosyringone (49-hydroxy-3′,5′-dimethoxyacetophenone; Sigma) to achieve a final OD600 of 0.5 ...
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bioRxiv - Cancer Biology 2024Quote: ... 1402-9500) containing 3 μL of SMART-seq3 lysis buffer (0.5 μL PEG 8000 (40% solution, Sigma Aldrich, P1458), 0.03 μL Triton X-100 (10% solution) ...
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bioRxiv - Neuroscience 2023Quote: ... Germany) containing the steroid hormone RU486 (200 μg/ml, CAS No.: 84371-65-3, Sigma-Aldrich, St. Louis, MO) for two days.
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bioRxiv - Microbiology 2023Quote: ... 30 μL of MTT [(3- [4,5-dimethyl-2-thiazolyl] -2,5-diphenyl-2H-tetrazolium bromide; 5 mg/mL; (Sigma-Aldrich)] was added in each well and the plate incubated for 3 hours at 25°C ...
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bioRxiv - Microbiology 2023Quote: ... The coverslip was rinsed 3 times with PBS-T and incubated with Alexa Flour conjugated secondary antibodies (Sigma-Aldrich) and Hoechst 33342 (Sigma-Aldrich ...
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bioRxiv - Molecular Biology 2023Quote: Articular cartilage explants from the non-OA donors were treated with 3 μM of Wnt agonist CHIR99021 (Sigma-Aldrich) or with 1 μL/mL of its vehicle (V ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... and MTT reagent (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) were purchased from Sigma-Aldrich (St Louis, MO). Phalloidin-iFluor 594 was purchased from Abcam ...
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bioRxiv - Cell Biology 2023Quote: ... The membrane was incubated with blocking buffer (PBS containing 0.1% Triton X-100 and 3% fatty-acid-and globulin-free BSA [A7030, Lot No. SLCH7959, SIGMA]) for 1 h at room temperature (approximately 26-28°C ...
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bioRxiv - Microbiology 2024Quote: ... Coloring reactions were performed with nitro blue tetrazolium/5-Bromo-4-chloro-3-indolyl phosphate (NBT/BCIP) solution (Sigma), and then sections were washed in PBS ...
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bioRxiv - Microbiology 2024Quote: ... after which the remaining supernatant (2 x 45ml) was collected and concentrated over a 3 kDa Amicon filter (Millipore). The residue was suspended in a volume of 900 µl ...
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bioRxiv - Molecular Biology 2024Quote: ... were incubated with 3 µg of D-Val-Leu-Lys 4-nitroanilide dihydrochloride chromogenic substrate (S-2251) (Sigma-Aldrich) in the presence of 1 µg of rFhENO or 1% BSA as negative control in a test volume of 100 µL ...
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bioRxiv - Microbiology 2024Quote: ... Liver and spleen samples from experimentally infected fish for 3 days were fixed in 4% paraformaldehyde (PFA, Sigma, USA), embedded in paraffin and sectioned (4 μm ...
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bioRxiv - Neuroscience 2024Quote: ... before being cut into 250μm parasagittal slices using a McIlwain tissue chopper and placed on Millicell membrane (3 to 4 slices each per animal, 0.4 μm membranes, Merck Millipore) in 50% BME (41010026 ...
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bioRxiv - Molecular Biology 2023Quote: ... pregnant females were ovariectomized on E2.5 and afterwards injected with 3 mg medroxyprogesterone 17-acetate subcutaneously (Sigma, M-1629) on E3.5 and E5.5 ...
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bioRxiv - Neuroscience 2024Quote: ... cells grown in 96-well plates were treated with 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT, Sigma, Hungary) in a final concentration of 200 μg/ml ...
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bioRxiv - Microbiology 2024Quote: ... The female individuals were anesthetized with 0.02% (w/v) ethyl 3-aminobenzoate methanesulfonate (Sigma-Aldrich, St. Louis, MO, USA), euthanized by decapitation ...
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bioRxiv - Molecular Biology 2024Quote: ... were grown for 2 hours on 3 ml of LB with 0.2% arabinose before placing them on a patch consisting of 1% agarose (Sigma) in water ...
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bioRxiv - Biochemistry 2024Quote: ... and 3 µg standards (manually mixed, Avanti Polar Lipids® and Sigma-Aldrich®; BVO from Spectrum Chemical®) onto a Merck HPTLC glass plate silica gel 60 (20×10cm ...
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bioRxiv - Microbiology 2024Quote: ... Oat seedlings for purification and amplification were treated with 3 mM maleic hydrazide (Sigma-Aldrich, St. Louis, MO, USA) at emergence ...
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bioRxiv - Cell Biology 2024Quote: ... permeabilized using 0.2% Triton-X100 in 1x phosphate buffered saline (PBS) for 15 min and blocked using 3% BSA (Sigma) in 1x PBS for 30 min ...
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bioRxiv - Biophysics 2023Quote: Tricaine stock was prepared by dissolving 400 mg of Tricaine powder (ethyl 3-aminobenzoate methanesulfonate; Millipore Sigma, #E10521-50G) with 97.9 ml of deionized water and 2.1 ml of 1 M Tris ...
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bioRxiv - Neuroscience 2024Quote: ... The device was then pulsed with 3 bolus each of 4 different concentrations of dopamine hydrochloride (Sigma, H8502-10G), 0.5 μM ...