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Citations for Millipore Sigma :
7751 - 7800 of 9891 citations for SUMO2 3 Blocking Peptide since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2021Quote: ... 3-trimethylsilyl propionic acid (TSP) and 2,2,2-trifluoroethanol (TFE-d3) of analytical grade were obtained from Sigma-Aldrich (Ireland).
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bioRxiv - Biochemistry 2021Quote: ... Elution fractions containing the protein of interest were concentrated using a centrifugal filter (Amicon Ultra, MWCO 3 kDa, Sigma) before loading onto a size-exclusion Sepharose 200 column (Cytiva ...
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bioRxiv - Biochemistry 2021Quote: ... CGI-99(2-101)-containing peak fractions were concentrated using a centrifugal filter (Amicon Ultra, MWCO 3 kDa, Sigma) to 24 mg ml−1 ...
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bioRxiv - Molecular Biology 2021Quote: ... gallinamide A-bound cruzain was concentrated to 10 mg/mL and the activation buffer was exchanged to 2 mM Bis-Tris pH 5.8 using a centrifugal filter with molecular weight cut-off of 3 kDa (Millipore). For cruzain-gallinamide A analog complexes ...
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bioRxiv - Neuroscience 2021Quote: ... Sections were positioned on gelatin-coated slides to allow further development with 3:1 ammonia solution (1054261000, Milipore Sigma) for 8 minutes ...
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bioRxiv - Developmental Biology 2021Quote: ... 293T or AL1 cells were incubated for 3 hours with anti-Myc mouse monoclonal antibody (Sigma-Aldrich; 1:500), and secondary staining was performed using anti-mouse AlexaFluor568 antibodies (Invitrogen ...
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bioRxiv - Developmental Biology 2021Quote: ... the media was replaced with 5mL of digestive enzyme mixture (3 mg/mL collagenase I (Cat.No. C0130; Sigma-Aldrich), 3 mg/mL collagenase II (Cat.No ...
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bioRxiv - Plant Biology 2021Quote: ... N-hexanoyl-L-homoserine lactone (C6-HSL) and N-3-oxotetradecanoyl-L-homoserine lactone (oxo-C14-HSL) (Sigma-Aldrich) at concentrations as indicated were used in this study.
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bioRxiv - Pathology 2021Quote: ... the lyophilized antibody panel was suspended in antibody diluent buffer (TBS-IHC tween, Donkey serum 3%) and filtered with a 0.1 µm filter (Millipore).
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bioRxiv - Developmental Biology 2020Quote: ... were post-fixed with 4% para-formaldehyde and blocked with PBS solution containing 0.3% Triton-X 100 and 3% normal Donkey serum (Sigma). Antibody dilution buffer consisted of PBS containing 1% BSA and 0.3% Triton-X 100 ...
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bioRxiv - Cancer Biology 2021Quote: ... The 24h-conditioned medium was then concentrated to 0.2 mg/ml through Amicon filters (3 kDa cut-off, Millipore), and incubated in cleavage buffer with or without pepstatin A (12.5 µM ...
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bioRxiv - Biophysics 2020Quote: ... the polymerization was catalysed by adding 3 % (v/v) N,N,N’,N’-tetramethylethylenediamine (TEMED, Sigma, St. Louis, USA) and the emulsion was degassed again for 6 min ...
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bioRxiv - Microbiology 2020Quote: ... 2 mL of 0.3% agarose in DMEM supplemented with 2% FBS or 3 mL of 0.6 or 1.2 percent microcrystalline cellulose (MCC; Sigma 435244) in serum-free DMEM was added ...
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bioRxiv - Immunology 2021Quote: ... in conjunction with pLKO.1 lentiviral vector targeting GFP or human c-Rel at a ratio of (1.5:3:4) using X-tremeGENE HP DNA transfection reagent (Sigma). 48h and 72h later viral particle containing media was harvested ...
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bioRxiv - Microbiology 2021Quote: ... The labeled phages were gently shaked at room temperature for 3 hours and washed with an Amicon ultra-0.5 centrifugal filter unit (100kD, Millipore) in phage storage buffer (100mM NaCl ...
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bioRxiv - Molecular Biology 2020Quote: ... or and 3) without 2i and LIF supplemented with 0.25 M all-trans-retinoic acid (Sigma-Aldrich, Cat #R2625), and cultured for 4 days.
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bioRxiv - Molecular Biology 2020Quote: ... Cell disruption was carried out by vortexing (3 cycles, 5 min each) at 4°C using 0.2 ml of glass beads (425-600 μm; Sigma). For the Rpb3 immunopurification ...
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bioRxiv - Microbiology 2021Quote: ... Fluids from a hydrothermal plume above Anemone and background seawater were collected using a Seabird SBE911 CTD and 10 L Niskin bottles and 3 L of the plume and seawater fluid was filtered through 0.22 μm Sterivex filters (Millipore). DNA was extracted from the filters using a phenol-chloroform method adapted from Crump et al ...
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bioRxiv - Immunology 2019Quote: ... Blisters were pierced on their lateral border using a 26·5G needle and exudates collected into tubes containing 50μl 3% sodium citrate (Sigma) in PBS (Gibco ...
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bioRxiv - Immunology 2020Quote: ... cells were fixed in 1% PFA (10 min, 37°C) and permeabilized in permeabilization buffer (PBS 3% BSA, 0.1% saponin, Sigma) for 5 min ...
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bioRxiv - Microbiology 2021Quote: ... 200 μL of lysis buffer (8M urea in 50mM Tris-Cl pH 7.5, supplemented with phosphatase inhibitor cocktail 3 [Millipore-Sigma]) was added to each of the pellets and pipetted up and down several time to lyse a portion of the pellet ...
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bioRxiv - Molecular Biology 2020Quote: ... The target protein was eluted with 20 ml elution buffer 3 (600 mM NaCl, 20 mM Tris-HCl, pH 7.4, 2.5 mM desthiobiotin (Sigma-Aldrich), and 1 mM DTT).
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bioRxiv - Cell Biology 2021Quote: ... cells were washed 3 times in PBS and blocked in PBS supplemented with 0.5 % fish skin gelatin (Sigma Aldrich) for 20 min ...
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bioRxiv - Genetics 2020Quote: ... incubated for 3 hours at 25 °C with the secondary antibody (anti-rabbit IgG peroxidase 1:10000, Sigma-Aldrich; anti-mouse IgG peroxidase 1:10000 ...
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bioRxiv - Immunology 2021Quote: ... Wells were washed and spots were detected by adding a filtered solution of 0.2mg/mL AEC (3-amino-9-ethylcarbazole; Sigma) in 0.1M sodium acetate buffer [pH 5.0] and 0.05% hydrogen peroxide (Sigma) ...
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bioRxiv - Bioengineering 2021Quote: ... and 1H-NMR spectra were recorded on a Varian Unity-300 (300MHz) NMR spectrometer (Varian Inc.) using 3-(trimethylsilyl)propionic acid-d4 sodium salt (0.05 w/v%, Sigma) as an internal standard (Figure S20 and Table S1).
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bioRxiv - Neuroscience 2021Quote: ... animals received daily intraperitoneal (I.P) injections of L3,4-dihydroxyphenylalanine methyl (L-DOPA, 3 day at 3mg/kg, then 6mg/kg, Sigma-Aldrich) and the peripheral DOPA decarboxylase inhibitor bensezaride hydrochloride (12mg/kg ...
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bioRxiv - Cancer Biology 2021Quote: ... migrating/invading cells were incubated with 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT; 5 mg/ml, 1/10 volume; Sigma-Aldrich) added to the culture medium at 37°C for 4h ...
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Disparate regulation of imd drives sex differences in infection pathology in Drosophila melanogasterbioRxiv - Immunology 2020Quote: Each sample consisted of 3 flies that were homogenised in 75μl of TE + 0.1% Triton X-100 (Sigma Aldrich). Samples were incubated for 20 min at 75°C and stored at −80°C ...
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bioRxiv - Neuroscience 2022Quote: ... On selected sections, immunoreactivities were tested: SATB1 (mouse, 1:500, SantaCruz) and GluR 2/3 (rabbit, 1:500, Millipore) were diluted in 0.1 M PB and incubated overnight at room temperature ...
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bioRxiv - Neuroscience 2022Quote: ... The larvae anesthetized with tricaine methanesulfonate were embedded in 3% agarose (low gelling temperature Type VII-A, Sigma-Aldrich) in 1/10 Evans solution ...
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bioRxiv - Immunology 2022Quote: ... and blocked for 1 hour at room temperature with 100 μL of 3% globulin free BSA (Sigma-Aldrich, A7030). After blocking ...
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bioRxiv - Molecular Biology 2022Quote: ... 3 μL of each sample was used as input for the NAD/NADH Quantitation Kit (Sigma-Aldrich, cat# MAK037) according to the instructions provided by the manufacturer ...
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bioRxiv - Developmental Biology 2022Quote: ... fused gastruloids were induced by administration of 3 μM CHIR and if indicated with doxycycline (1 μg/μl, Sigma) for 24 h ...
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bioRxiv - Molecular Biology 2022Quote: ... washed in PBS and incubated in buffer A (10 mM Tris pH 7.5, 2 mM MgCl2, 3 mM CaCl2, Sigma cOmplete EDTA-free Protease Inhibitor Cocktail ...
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bioRxiv - Neuroscience 2022Quote: ... Cells were washed 3 x in PBS and mounted on a glass coverslip in DAPI antifade solution (Sigma-Aldrich). Confocal microscopy was performed using the LSM800 (Zeiss ...
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bioRxiv - Biochemistry 2022Quote: ... The incubated beads were washed 3 times with HAC buffer followed by eluted in 1 M imidazole (Sigma, #IX0005) in HAC buffer.
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bioRxiv - Biochemistry 2022Quote: ... Yeast pellets were resuspended in 400 μL FLAG lysis buffer (20 mM HEPES, pH 7.4, 100 mM NaCl, 10% [w/v] glycerol, 3% [w/v] digitonin [Sigma: D141] ...
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bioRxiv - Animal Behavior and Cognition 2022Quote: ... as for the “Group two-choice assay” except using a single 3 cm diameter Petri dish containing 0.67% agarose and 150 mM sucrose (Sigma) onto which a non-accessible container covered with a fabric gauze and a perforated cap of a 15 ml Falcon tube (diameter 1 cm ...
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bioRxiv - Bioengineering 2022Quote: ... Collected samples of conditioned medium were filtered (0.2 μm) and then concentrated ten-fold (Amicon Ultra centrifugal filters; 3 kDa cut off; Millipore).
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bioRxiv - Bioengineering 2022Quote: ... sections were rinsed with PBS 3 times and then mounted on microscope glass slides with Mowiol (Sigma-Aldrich, 81381). The expression of proteins was visualized with a Leica TCS SP5X microscope and images were processed with ImageJ (version 1.53f51) ...
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bioRxiv - Developmental Biology 2022Quote: ... with 3 mg/mL of bovine serum albumin (BSA) and 100 µM of IBMX (Sigma-Aldrich, Merck, Darmstadt, Germany), and collected ...
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bioRxiv - Cell Biology 2022Quote: ... the pellet was resuspended in 3 mL Nuclear Permeabilization Buffer (1X PBS, 5% Bovine Serum Albumin, 0.2% IGEPAL CA-630 (Sigma), 1 mM DTT ...
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bioRxiv - Cell Biology 2022Quote: ... was revealed with 5-bromo-4-chloro-3-indolyl β-D galactoside (X-gal) (Sigma-Aldrich, Carlsbad, CA, USA). Diploid cells were grown on SC medium supplemented with 20 mg/L adenine hemisulfate ...
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bioRxiv - Cell Biology 2022Quote: ... activated eggs were cultured for 3 additional hours in KSOM + amino acids media (Sigma Aldrich, Cat# MR-106-D). For parthenogenetic activation of eggs ...
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bioRxiv - Cancer Biology 2022Quote: ... Cells were incubated with 50 µL of a 2 mg/mL solution of (3-(4,5-dimethyl-thiazole-2-yl)-2,5-biphenyl tetrazolium (MTT, Sigma-Aldrich) in PBS for 4 h and then exposed to 100 µL dimethyl sulfoxide (DMSO ...
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bioRxiv - Plant Biology 2022Quote: ... After addition of 10μl of 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide (MTT) solution (5mg/ml, Sigma-Aldrich, USA) the cells were incubated in darkness for another 4 hrs ...
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bioRxiv - Microbiology 2022Quote: Cells were then pelleted at 7000 × g for 3 min and resulting supernatants filtered through a 0.2 μm PVDF filter (Millipore). The no bacteria “fresh” 5-FU controls were also filtered in this way to account for any potential loss of 5-FU by filtration ...
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bioRxiv - Microbiology 2022Quote: The effect of CAPG depletion on viability of KO and siRNA-treated cells was assessed at several timepoints using a 3-[4,5-dimethylthiazol-2-yl]-2,5 diphenyl tetrazolium bromide (MTT) assay (11465007001; Sigma-Aldrich) per manufacturer directions ...
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bioRxiv - Neuroscience 2022Quote: Recordings were then performed in oxygenated aCSF superfused at a rate of 2-3 ml/min and supplemented with 100 µM picrotoxin (Alomone Labs) and 0.5-1 µM Strychnine (Sigma). The internal solution was the same as above with 2-4 mM QX314 to block Na+ channels from driving AP firing ...