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Citations for Millipore Sigma :
7651 - 7700 of 9891 citations for SUMO2 3 Blocking Peptide since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2021Quote: ... sections were washed twice for 10-m in 1x PBS and then incubated for 1-hr in a blocking buffer containing 3-5% Normal Goat Serum (Jackson Immuno Research, 005-000-121) and 0.01-0.03% Triton-X (Sigma, X100), made up in 1x PBS ...
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bioRxiv - Biochemistry 2021Quote: ... The following mobile phases were used for FIA-MS scouting: 20 mM formic acid pH 3 (Sigma-Aldrich F0507), 10 mM ammonium acetate pH 5 (Sigma-Aldrich 73594) ...
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bioRxiv - Neuroscience 2021Quote: ... frozen tissue (from 2–3 mice or 1 rat) was homogenized in Lysis Buffer (EZ Prep Nuclei Kit, Sigma) with Protector RNAase Inhibitor (Sigma ...
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bioRxiv - Neuroscience 2021Quote: ... Fish were deeply anesthetized with 0.2% 3-aminobenzoic ethyl ester (MS-222; Sigma-Aldrich, St. Louis, MO; RRID: SCR_008988) in water just before surgery or tissue preparation.
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bioRxiv - Neuroscience 2021Quote: ... and posteriorly permeabilized and blocked against nonspecific binding using a solution of 3% bovine serum albumin (BSA) (Sigma-Aldrich) and 0.1% Triton X-100 (Sigma-Aldrich ...
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bioRxiv - Cancer Biology 2021Quote: ... and then concentrated using 15 ml Amicon spin filter cartridges (cutoff: 3 kDa, 10705884-Merck Millipore, Burlington, MA, USA) by serial addition of 10 ml of CM and centrifugation at 4000 x g until 1.5 ml were left ...
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bioRxiv - Biochemistry 2021Quote: ... One milliliter of the N-oxide solution was added dropwise to 3 mL of sodium acetate (37.5 mM; pH 5.3; Sigma Aldrich), followed by incubation at room temperature for 24 hours ...
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bioRxiv - Molecular Biology 2021Quote: ... The unbroken cells were removed by centrifugation (3,000 x g; 4°C; 5 min; Sigma 3-16KL; rotor 11180). The membrane fraction was pelleted down by high-speed centrifugation (100,000 x g ...
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bioRxiv - Neuroscience 2020Quote: ... Band intensity was measured as the sum optical density by using ImageJ software (version1.4; NIH) and normalized to control labelling of glyceraldehyde 3-phosphate dehydrogenase (GAPDH; 1:5000, Millipore).
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bioRxiv - Microbiology 2021Quote: ... the lysates were incubated for 3 h with 50 μl anti-FLAG packed agarose affinity gel (A-2220; Sigma) at 4 °C with rotation ...
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bioRxiv - Biophysics 2019Quote: ... The eluate was incubated for 3 h at 4°C with 5 mL of FLAG M2 Agarose beads (Sigma), collected in a glass column ...
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bioRxiv - Neuroscience 2022Quote: ... N2a NM-HAagg (EP) donor cells were treated for 3 d with methyltransferase inhibitors 5-Azacytidine (Aza) (Sigma-Aldrich), Decitabine (Dec ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... 250 µL X-Gal (5-bromo-4-chloro-3-indolyl-β-D-galactoside) (20 mg/ml) (Sigma-Aldrich, UK) and Isopropyl ß-D-1-thiogalactopyranoside (IPTG ...
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bioRxiv - Molecular Biology 2022Quote: ... 1-3 million cells were treated for 24 hours with 1.2 uM aphidicolin in PBS (Millipore Sigma cat#: A0781). Cells were attached to Concanavalin A coated slides for one hour at room temperature ...
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bioRxiv - Biophysics 2022Quote: ... The primary antibody (1:100 Anti-Tau [3-repeat isoform RD3] Antibody, clone 8E6/C11, 05-803, Sigma-Aldrich and 1:100 Anti-Tau [4-repeat isoform RD4] Antibody ...
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bioRxiv - Microbiology 2022Quote: ... A total of 500 μL of normalized culture was mixed with 100 μL of 1% Zwittergent 3-14 (Sigma) in triplicate in 100 mM citric acid and incubated at 50 °C for 20 min ...
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bioRxiv - Systems Biology 2022Quote: ... Cultures were treated with the indicated amount of 1-(1,1-dimethylethyl)-3-(1-naphthalenyl)-1H-pyrazolo[3,4-d]pyrimidin-4-amine (1-NM-PP1; Millipore Sigma), taken from a 10,000x stock in DMSO ...
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bioRxiv - Neuroscience 2022Quote: A model of hyperalgesic priming was used to induce chronic widespread pain in mice with consecutive injections of carrageenan into the right gastrocnemius muscle 4 days apart (i.m. 3% 30μl, Sigma Aldrich). Control mice only received one injection on D0 (30).
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bioRxiv - Immunology 2022Quote: ... and concentrated until ~1.5 mg/ml using Amicon Ultra-15 Centrifugal Filter Unit with a 3 kDa cut-off (Millipore). Protein concentration was determined by BCA assay (Pierce/Thermo Scientific).
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bioRxiv - Immunology 2022Quote: ... MSCs were frozen at passage 3 with 1.25*106 cells/ml in freezing medium containing FBS (BCBV7611, Sigma-Aldrich) with 10% DMSO and stored in liquid nitrogen until further use ...
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bioRxiv - Immunology 2022Quote: ... and either plate-bound 3 μg/mL mouse IgG1 isotype control or anti-FLAG mAb (clone M2, Sigma-Aldrich) in complete RPMI-1640 culture medium supplemented with 10 μM BrdU ...
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bioRxiv - Immunology 2022Quote: ... and either plate-bound 3 μg/mL mouse IgG1 isotype control or anti-FLAG mAb (clone M2, Sigma-Aldrich) in complete RPMI-1640 culture medium ...
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bioRxiv - Cell Biology 2022Quote: ... The remaining pellet was resuspended in 500 μL bovine serum albumin (BSA, 3% solution in PBS, Sigma-Aldrich, A3294) and centrifuged again at 1000xg at 4°C for 10 minutes ...
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bioRxiv - Cell Biology 2022Quote: ... – to synchronise cells at the G2/M boundary - or with a 3-hour treatment with STLC (10μM, Sigma-Aldrich) - to arrest cells in mitosis with monopolar spindles ...
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bioRxiv - Neuroscience 2022Quote: ... then selected after 24-48 hours for 3 to 14 days with 8 µg/mL of puromycin (Sigma-Aldrich). The iPSCs with a stably integrated human NIL under a tetracycline-inducible promoter were exposed to 2 µg/mL doxycycline in neuronal induction medium (NIM) ...
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bioRxiv - Bioengineering 2022Quote: ... a differentiation medium (reduced serum + additives) adapted from Messmer et al.3 – reduced serum with insulin (Sigma Aldrich #I0516), 1-oleoyl lysophosphatidic acid (LPA ...
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bioRxiv - Developmental Biology 2022Quote: ... 50,000 of cells for each experimental replicate and/or condition were resuspended in lysis buffer (10 mM Tris-HCl, pH 7.4, 10 mM NaCl, 3 mM MgCl2 and 0.1% IGEPAL CA-630, Sigma aldrich) to perform crude nuclei isolation ...
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bioRxiv - Cell Biology 2019Quote: ... 3% goat serum) and then incubated with mouse anti-tropomyosin-5 antibody (1:200; clone 2G10.2, Millipore, Burlington, MA) which recognizes Tpm3.1 and Tpm3.2 ...
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bioRxiv - Microbiology 2019Quote: ... we filtered the protein suspension through a 0.22 mm syringe filter and transferred into a 3 kDa cutoff Amicon Ultra-15 filter unit (Millipore) for protein concentration ...
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bioRxiv - Genetics 2019Quote: ... Conditional AID degron strains were grown in YPD or YPG and auxin (3-indoleacetic acid; Sigma Aldrich Cat. I3750) at a final concentration of 500µM to deplete AID-tagged proteins ...
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bioRxiv - Biochemistry 2020Quote: ... The empty liposomes were prepared from a mix of DOPC (1,2-Dioleoyl-sn-glycero-3-phosphocholine) (Avanti lipids) and CHS (Sigma). DOPC and CHS were dissolved in chloroform at a 10:1 (wt:wt ...
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bioRxiv - Microbiology 2019Quote: ... about 12 liters of seawater were prefiltered through a 200 µm nylon mesh to remove large plankton and then sequentially filtered using a peristaltic pump through a 20 µm nylon mesh and then through 3 µm and 0.2 µm pore-size polycarbonate filters of 142 mm diameter (Isopore, Millipore). Filtration time was about 15-20 minutes ...
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bioRxiv - Biophysics 2019Quote: All zebrafish were sacrificed by immersion in ethyl 3-aminobenzoate methanesulfonate (MS-222, 0.1% in PBS, Sigma-Aldrich, A5040) until five minutes after the respiratory movement of the opercula stopped ...
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bioRxiv - Cell Biology 2019Quote: ... zebrafish were anesthetized in tank system water with 0.1mg/mL tricaine (ethyl 3-aminobenzoate methanesulfonate, Sigma Aldrich, Hamburg, Germany) and subsequently placed onto a foam surgical stand for surgery ...
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bioRxiv - Microbiology 2019Quote: ... 3 wells of parasites and 1 well of the RBC control were treated with 700nM dihydroartemisinin (DHA) (Sigma-Aldrich); an additional 3 wells of parasites and 1 well of RBC control was treated with an equivalent amount of dimethyl sulfoxide (DMSO ...
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bioRxiv - Bioengineering 2020Quote: ... This assay relies on the reduction and conversion of yellow 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium-bromide (MTT) reagent (#M2128, Sigma-Aldrich) into purple formazan salt ...
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bioRxiv - Biochemistry 2020Quote: ... Cells were incubated with 5 mg/mL of 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT, Sigma Aldrich) in PBS for 15 min at 37 °C ...
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bioRxiv - Biochemistry 2019Quote: ... cells were grown at 25°C and treated with either 0.5 mM indol-3-acetic acid (IAA, I2886, Sigma) or ethanol ...
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bioRxiv - Pharmacology and Toxicology 2019Quote: Plasma and liver samples were prepared as previously described (18) with 3:1 ice-cold acetonitrile and 2.5µM chlorpropamide internal standard (Sigma Aldrich) then run on both the Waters ACQUITY UPLC HSS T3 (1.8µm particle size ...
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bioRxiv - Genetics 2019Quote: ... The total cleared lysate for each sample was pooled from 3 tubes (3,825 µl total) and added to 80 µl pre-equilibrated anti-V5 agarose beads (Sigma), and incubated in loBind tubes (Eppendorf ...
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bioRxiv - Developmental Biology 2019Quote: ... expression of Flag-tagged UNC-3 in the total cell lysate was detected using mouse anti-Flag (Sigma, #F3165), expression of Flag-tagged UNC-3 in the IP was detected using rabbit anti-Flag (Sigma ...
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bioRxiv - Biochemistry 2019Quote: ... They were then washed with TNC buffer containing 0.05 % Tween 20 (TNC-T) and blocked with 3% bovine serum albumin (Sigma) in TNC-T buffer ...
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bioRxiv - Cell Biology 2019Quote: ... appropriate amounts (calculated by approximating the average abundance of every fatty acid within the sample and adding matching amounts of standard) of 1,2-diundecanoyl-sn-glycero-3-phosphocholine (phospholipid standard, 850330C, Sigma) were included in extraction mixture as internal standard ...
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bioRxiv - Cell Biology 2019Quote: ... Layer 2/3 pyramidal neurons were loaded iontophoretically with a 0.4 % Lucifer yellow solution (Sigma-Aldrich, St. Louis, MO) using sharp micropipettes and a negative current of 70 nA until the dendrites were fluorescing brightly ...
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bioRxiv - Microbiology 2019Quote: ... Cells were harvested by centrifugation and lysed by sonication in Buffer A (25 mM HEPES pH 7.2, 500 mM NaCl, 3 mM DTT) with cOmplete mini protease inhibitor (Sigma) added ...
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bioRxiv - Pharmacology and Toxicology 2019Quote: ... The larvae were then anesthetised with 0.04% MS-222 (Ethyl 3-aminobenzoate methane sulfonic acid salt 98%, Sigma Aldrich) and fixed in 4% PFA (Sigma Aldrich ...
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bioRxiv - Genetics 2019Quote: ... For the inhibition of global transcription (Supplementary Figure 3) 1,10-Phenanthroline (100 μg/mL in 20% ethanol, Sigma-Aldrich) [30] was added to cultures 3 hours after induction into the meiotic program ...
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bioRxiv - Bioengineering 2019Quote: ... Samples were then washed 3 times with TBS containing 1% BSA and 0.1% w/v sodium azide (Sigma Aldrich). After staining ...
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bioRxiv - Immunology 2020Quote: ... 50 μl of antibody exposed to hemin or hemin in PBS only were mixed with 150 μl of reaction buffer (0.15 M citrate-phosphate buffer pH 5) containing 0.9 mM of 2,2-Azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) diammonium salt (ABTS, Sigma-Aldrich) and 6 mM H2O2 ...
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bioRxiv - Molecular Biology 2020Quote: ... was obtained by the transfection of shRNA directed against the 3’ UTR region of Aurora-A (clone NM_003600.x-985s1c1, Sigma) followed by clonal dilution and selection of Aurora-A depleted cells with 2 μg/ml of puromycin.