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Citations for Millipore Sigma :
7951 - 8000 of 9891 citations for SUMO2 3 Blocking Peptide since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2023Quote: ... and microtubules (1 µM paclitaxel, T7402, Sigma Aldrich; 33 µM nocodazole, M1404, Sigma Aldrich, 3 different commercial lots tested). The effect of nocodazole during overnight/short-term culture was enhanced by 10 µM verapamil (V4629 ...
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bioRxiv - Genetics 2024Quote: ... Zygotes with visible pronuclei washed 3 times in HTF medium and transferred to KSOM medium (Millipore, MR-106-D) and cultured in low oxygen chamber filled with the mixture gas (5% O2/ 5% CO2) ...
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bioRxiv - Neuroscience 2023Quote: ... Germany) containing the steroid hormone RU486 (200 μg/ml, CAS No.: 84371-65-3, Sigma-Aldrich, St. Louis, MO) for two days.
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bioRxiv - Biochemistry 2024Quote: ... and 3 µg standards (manually mixed, Avanti Polar Lipids® and Sigma-Aldrich®; BVO from Spectrum Chemical®) onto a Merck HPTLC glass plate silica gel 60 (20×10cm ...
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bioRxiv - Neuroscience 2024Quote: ... microglia were treated with the respective concentrations of selected drugs (see Table 3) or DMSO (Sigma-Aldrich, Cat #:472301) as control and incubated for 6hrs and 24hrs before harvest for RNA extraction ...
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bioRxiv - Cancer Biology 2024Quote: ... 1402-9500) containing 3 μL of SMART-seq3 lysis buffer (0.5 μL PEG 8000 (40% solution, Sigma Aldrich, P1458), 0.03 μL Triton X-100 (10% solution) ...
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bioRxiv - Microbiology 2023Quote: Infected S10-3 cells seeded onto coverslips were fixed in 4% PFA and permeabilized in 0.1% Trion-X100 (Sigma). For co-detection of RNA and capsid ...
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bioRxiv - Microbiology 2023Quote: ... A Hybond N+ membrane (GE lifesciences, 45000854) and 6 sheets of 3 mm Whatman cellulose paper (Sigma Aldrich, WHA3030861) were pre- soaked for 5 minutes in room temperature 0.5X TBE ...
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bioRxiv - Immunology 2023Quote: ... A total of 500 μL of normalized culture was mixed with 100 μL of 1% Zwittergent 3-14 (Sigma) in triplicate in 100 mM citric acid and incubated at 50 °C for 20 min ...
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bioRxiv - Immunology 2023Quote: Bone marrow supernatants (0.5 mL per sample) were concentrated with 3 kDa MWCO Amicon Ultra Centrifugal filter devices (Merck Millipore) up to a final volume of 30 μL ...
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bioRxiv - Molecular Biology 2023Quote: ... in PBS for 10 min at room temperature in the dark and washed 3 times with PBS (#D8537, Sigma). PBS was completely removed and cells were left to dry for 5 min at room temperature ...
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bioRxiv - Biophysics 2023Quote: ... Samples were concentrated to an A260ml−1 of 3 (100 nM) using an Amicon Ultra 0.5 ml spin column with a 100 kDa cutoff (Merck Millipore) to an OD260 absorbance of 5.0.
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bioRxiv - Cancer Biology 2023Quote: Culture medium was replaced with 100 µL medium containing 5 mg/mL filtered 3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) (#M5655, Sigma-Aldrich) and incubated for 2 h at 37°C ...
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bioRxiv - Biophysics 2023Quote: ... Samples were measured in a 3 mm path-length cuvette (Hellma, 105.251-QS) in PBS (Sigma-Aldrich, D8537-500ML) containing 500 mM NaCl adjusted at pH 7.4.
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bioRxiv - Biophysics 2023Quote: ... the fluorescence spectra of mRaichu were measured in solutions containing 3 mg/mL BSA (SIGMA, Product No. 017-25771), 100 mM NaCl ...
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bioRxiv - Microbiology 2023Quote: ... and the metal-loaded polymer purified and washed in C-buffer using an Amicon Ultra-0.5 centrifugal filter unit with 3 kDa cutoff (Millipore-Sigma). At the same time ...
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bioRxiv - Molecular Biology 2023Quote: Articular cartilage explants from the non-OA donors were treated with 3 μM of Wnt agonist CHIR99021 (Sigma-Aldrich) or with 1 μL/mL of its vehicle (V ...
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bioRxiv - Microbiology 2023Quote: ... The coverslip was rinsed 3 times with PBS-T and incubated with Alexa Flour conjugated secondary antibodies (Sigma-Aldrich) and Hoechst 33342 (Sigma-Aldrich ...
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bioRxiv - Biophysics 2023Quote: Tricaine stock was prepared by dissolving 400 mg of Tricaine powder (ethyl 3-aminobenzoate methanesulfonate; Millipore Sigma, #E10521-50G) with 97.9 ml of deionized water and 2.1 ml of 1 M Tris ...
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bioRxiv - Cancer Biology 2023Quote: ... Tricaine stock was prepared by dissolving 400 mg of Tricaine powder (ethyl 3-aminobenzoate methanesulfonate; Millipore Sigma, #E10521-50G) with 97.9 ml of deionized water and 2.1 ml of 1 M Tris ...
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bioRxiv - Plant Biology 2023Quote: ... 100 mg glass wool were filled with 200 µl (Z)-3-hexenyl acetate (HAC; >98%; Sigma-Aldrich, Buchs, Switzerland) diluted 50 fold in EtOH and sealed with screw caps containing a rubber septum ...
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bioRxiv - Microbiology 2023Quote: ... 30 μL of MTT [(3- [4,5-dimethyl-2-thiazolyl] -2,5-diphenyl-2H-tetrazolium bromide; 5 mg/mL; (Sigma-Aldrich)] was added in each well and the plate incubated for 3 hours at 25°C ...
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bioRxiv - Plant Biology 2024Quote: ... They were then resuspended in 10 mM MES-KOH buffer (pH 5.7) with 10 mM MgCl2 and 100 mM acetosyringone (49-hydroxy-3′,5′-dimethoxyacetophenone; Sigma) to achieve a final OD600 of 0.5 ...
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bioRxiv - Cell Biology 2023Quote: ... Pooled bone marrow stromal cells (BMSCs) were washed with 3 times with PBS and cultured in αMEM (Sigma, M2279) supplemented with 20% FBS ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... 99.7% pure), Bisphenol TMC (Catalog #, 52746-49-3) and 2′,7′-Dichlorodihydrofluorescein diacetate (Catalog #, D6883) were purchased from Millipore Sig ma (MO ...
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bioRxiv - Cell Biology 2024Quote: ... The small interfering siRNA (siRNA) oligonucleotide targeting human FCHSD2 (5’-GCAUACUCCUGAGACCUCA[dT][dT]-3’) was obtained from Sigma Aldrich. FCHSD2 siRNA knockdown in all HeLa cell lines was performed for 48 h using the DharmaFECT transfection reagent (Dharmacon ...
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Genetic gradual reduction of OGT activity unveils the essential role of O-GlcNAc in the mouse embryobioRxiv - Developmental Biology 2024Quote: ... and then blocked for 3 hr at room temperature or overnight at 4 °C in BSA (Sigma-Aldrich A2153) 3% in PBS-T ...
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bioRxiv - Developmental Biology 2024Quote: ... they were first anesthetized using egg water containing 0.016% tricaine (3-amino benzoic acid ethyl ester) from Sigma-Aldrich. Subsequently ...
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bioRxiv - Developmental Biology 2024Quote: ... 50-60 µm cryosections were blocked for 3h at room temperature in PBS containing 3% BSA (A7906, Sigma-Aldrich) and 0.5%Triton X-100 (T9284 ...
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bioRxiv - Neuroscience 2024Quote: ... and the chocolate-flavored pellets were replaced with cotton pads infused with 1 ml of a 1% solution of 2,3,5-trimethyl-3-thiazoline (TMT, Sigma Aldrich) in water ...
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bioRxiv - Cell Biology 2024Quote: ... The chips were then washed with PBS 3 times before incubation with 0.5% (v/v) glutaraldehyde (Sigma-Aldrich, G6257) in PBS at RT for 10-15 min ...
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bioRxiv - Biophysics 2024Quote: ... Excised tissues are first allowed to fold into spheroids and seal in HM for 3-6 hours and are then embedded in 0.5% low gelling point agarose (Sigma) prepared in HM to reduce tissue movement during imaging ...
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bioRxiv - Systems Biology 2024Quote: ... UK) previously coated with calf- skin collagen (15 µg/cm2 and fibronectin 3 µg/cm2; both Sigma-Aldrich, UK), were transfected with one of three different commercial siRNA sequences designed to target the EGFR or an Allstars negative control siRNA sequence (final concentration 1 nM ...
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bioRxiv - Systems Biology 2024Quote: ... UK) previously coated with calf- skin collagen (15 µg/cm2 and fibronectin 3 µg/cm2; both Sigma-Aldrich, UK). The permeability of hCMEC/D3 cell monolayers to 70 kDa FITC-dextran (2 mg/ml ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... media was aspirated from cells and replaced with 5 mg/mL 3-(4,5-Dimethyl-2-thiazolyl)-2,5-diphenyl-2H-tetrazolium bromide (MTT, #M5655, Sigma Aldrich) solution in base media ...
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bioRxiv - Molecular Biology 2024Quote: ... Samples were treated with RNase A (345 μL TE, 3 μL 10 mg/mL RNAse A (Sigma-Aldrich, R6513), 2 μL 20 mg/mL Glycogen (Roche ...
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Angiogenesis in the mature mouse cortex is governed in a region specific and Notch1 dependent mannerbioRxiv - Neuroscience 2024Quote: ... mice were intravenously injected with FITC-dextran (100µL of 3-5% w/v in 0.9% saline; 70kDa, Sigma #46945) or Texas Red dextran (100µL of 3% w/v in 0.9% saline ...
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bioRxiv - Neuroscience 2024Quote: Stock solutions (1mg/ml) of 1,1′-dioctadecyl-3,3,3′,3′-tetramethylindocarbocyanine perchlorate (DiI) in N,N-dimethylformamide (Sigma-Aldrich 468495) were stored at −20°C ...
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bioRxiv - Physiology 2024Quote: ... The PDMS scaffold surface was activated oxygen plasma and then treated with a 5% (3-aminopropyl) triethoxysilane (Sigma-Aldrich) solution in methanol for 45 min ...
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bioRxiv - Neuroscience 2024Quote: ... Free-floating organotypic slices or vibratome sections were incubated in permeabilization solution (0.2% Triton-X100, 3% BSA in PBS; Sigma) for 1 hour at room temperature ...
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bioRxiv - Cell Biology 2024Quote: ... All the ASO probes were synthesized with biotin-TEG added to the 3’ end of the sequences (Sigma-Aldrich). Briefly ...
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bioRxiv - Cancer Biology 2024Quote: ... washed with PBS (3 times x 5 minutes) and incubated with secondary antibodies and Hoescht 33342 (Sigma-Aldrich, #14533) diluted in 1x Antibody diluent/Block solution for 2 hours at room temperature ...
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bioRxiv - Cancer Biology 2024Quote: ... mitotically active cells were identified using a rabbit anti-phosphohistone-3 antibody (Ser10, Millipore 06–570, 1:500 dilution), with a Cy3-donkey anti-rabbit immunoglobulin G secondary antibody (the Jackson laboratories ...
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bioRxiv - Molecular Biology 2024Quote: ... the culture medium was changed to medium supplemented with 500 μM 3-Indoleacetic acid (IAA; Sigma-Aldrich, I2886-5G) and 1 μg/mL doxycycline (Sigma-Aldrich ...
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bioRxiv - Cancer Biology 2024Quote: ... We used 3 and 5wt% GelMA with a 1:16 mass ratio of photoiniator (Lithium phenyl-2,4,6-trimethylbenzoylphosphinate, LAP; Sigma Aldrich). LAP and GelMA were dissolved together in Dulbecco’s Phosphate Buffered Saline (DPBS ...
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bioRxiv - Microbiology 2024Quote: ... The cell nucleus was stained with DAPI (1:1000 dilution, Sigma-Aldrich 28718-90-3, St. Louis, MO, USA) at room temperature for 15 min ...
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bioRxiv - Molecular Biology 2024Quote: ... Cells were washed 3 x in TBS-Tween (0.1%) + 2% BSA before incubation in TRITC conjugated anti-Mouse secondary antibody (Sigma) at 1:1000 concentration for 1 hr in the dark ...
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Evolution of polyamine resistance in Staphylococcus aureus through modulation of potassium transportbioRxiv - Microbiology 2024Quote: ... Four of the cultures were diluted 1/100 into fresh TSB containing 2mM of spermine (Sigma, 71-44-3), and two of the cultures were diluted 1/100 into plain TSB as a control ...
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bioRxiv - Molecular Biology 2024Quote: ... Cells were seeded at 1.0E5 cells/ml with 10 ng/ml IL-3 and 8μg/ml polybrene (Sigma-Aldrich). Virus was added to wells at 0 ...
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bioRxiv - Cell Biology 2024Quote: ... Dried lipids were resuspended in 15µL chloroform and 65µL of click mix containing 400µM 3-Azido-7-hydroxycoumarin (Baseclick #BCFA-047-1) and 900µM tetrakis(acetonitrile)copper(I) tetrafluoroborate (Sigma #677892) in acetonitrile/ethanol (7:3 ...