Labshake search
Citations for Millipore Sigma :
7701 - 7750 of 9891 citations for SUMO2 3 Blocking Peptide since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Pharmacology and Toxicology 2020Quote: ... and 12 mg RBD protein in 3 mL PBS was mixed with equal-volume Freund’s incomplete adjuvant (Sigma-Aldrich) for the second boosting ...
-
bioRxiv - Cell Biology 2020Quote: ... and gravity-sedimented for 3-5 min onto coverslips coated with poly-D lysine (Sigma-Aldrich, catalog number P1024), until cells became in contact with the glass ...
-
bioRxiv - Developmental Biology 2021Quote: ... and replated in neural maintenance media (NES) with 10μM Y27632 at 3×105 cells per cm2 on laminin (Sigma) coated surface ...
-
bioRxiv - Molecular Biology 2020Quote: ... Cells were broken by vortexing (3 cycles, 5 min each) using 0.2 ml of glass beads (425-600 μm; Sigma)) ...
-
bioRxiv - Molecular Biology 2020Quote: ... Embryos/larvae were kept in egg water (60 μg/ml Instant Ocean sea salts) at 28.5°C and treated with 0.02% ethyl 3-aminobenzoate methanesulfonate (Tricaine, Sigma-Aldrich) for anesthesia before imaging and fixation ...
-
bioRxiv - Microbiology 2021Quote: ... Wells with agarose plugs were fixed 3 days later with a solution of 2.5% formaldehyde (1635-4L, Sigma-Aldrich) in 3x PBS ...
-
bioRxiv - Neuroscience 2019Quote: ... aggregates were cultured in 3:1 medium supplemented with an additional 10% heat-inactivated FBS (Millipore, #es‒009‒b) and 100 µM taurine (Sigma ...
-
bioRxiv - Cell Biology 2020Quote: ... Cells were then cultured in adipogenic conditions (DMEM/10%FBS, 0.5 mM 3-isobutyl-1-methylxanthine, 1 μM dexamethasone (Sigma), and 10 μg/ml bovine insulin (Sigma) ...
-
bioRxiv - Pharmacology and Toxicology 2021Quote: ... 1.47 mM KH2PO4 supplemented with 5 mM DTT and concentrated to ∼10-20 mg/mL using 3 kDa Millipore concentrator and stored at -80 °C.
-
bioRxiv - Pharmacology and Toxicology 2021Quote: ... we carried out final desalting and buffer equilibration using ultrafiltration (Amicon centrifugal filters, 3-kDa molecular weight cutoff, Millipore). For oligonucleotides administered in PBS ...
-
bioRxiv - Neuroscience 2019Quote: ... Coronal sections were used for staining and washed 3 × 10 min in Triton PBS (PBS containing 0.3% v/v Triton X-100, Sigma). For visualization of STN borders we performed an immune-staining for the marker forkhead box protein P2 (FoxP2 ...
-
bioRxiv - Developmental Biology 2021Quote: ... EpiSCs were passaged 1:4–1:10 every 3 days by triturating the colonies into small clumps using 0.5 mg ml–1 collagenase IV (Sigma). MEK/WNT-inhibited RSCs were cultivated with 1,000 U ml–1 LIF ...
-
bioRxiv - Pharmacology and Toxicology 2020Quote: 36 hpf or 66 hpf embryos were anesthetized with 112 μg/mL 3-aminobenzoic acid ethyl ester (tricaine, Sigma), immobilized in 1% low melting-point agarose in the center of a 35 mm glass-bottomed dish (Corning) ...
-
bioRxiv - Synthetic Biology 2020Quote: ... Eluted proteins were exchanged into 20 mM sodium phosphate buffer containing 50 mM NaCl (pH 7.4) and concentrated using Amicon Ultra-15 centrifugal filters with a molecular weight cut-off of 3 kDa (Millipore) for STEPtag ...
-
bioRxiv - Microbiology 2020Quote: SARS-CoV-2 isolate nCoV-WA1-2020 (MN985325.1)18 (Vero passage 3) was kindly provided by CDC and propagated once in VeroE6 cells in DMEM (Sigma) supplemented with 2% fetal bovine serum (Gibco) ...
-
bioRxiv - Microbiology 2020Quote: ... 1 mM DTT) and treated with 3×45 s bead beating with glass beads (particle size≤106 μm Sigma) at maximum power and cooling on ice between the cycles (MP Biomedicals™ FastPrep-24™ ...
-
bioRxiv - Microbiology 2021Quote: ... The reaction was initiated at the spectrophotometer by adding anoxic xanthine oxidase (microbial; 1-3 µg/mL; Sigma-Aldrich) via syringe and needle through the septum lid ...
-
bioRxiv - Microbiology 2021Quote: ... and the resultant filtrates were concentrated using Amicon Ultra Centrifugal Filter Units with a 3 kDa cutoff (EMD Millipore). Protein concentration was quantified using the Bio-Rad protein assay (Bio-Rad Laboratories) ...
-
bioRxiv - Synthetic Biology 2019Quote: ... After rinsing 30 mL of 3% BSA TBST buffer with 0.0625 µL of anti-mouse antibody (Sigma-Aldrich, A9044) was added ...
-
bioRxiv - Developmental Biology 2020Quote: Cryosections (30-40 μm) were blocked with PBS/1-3% normal goat or donkey serum/0.3% Triton X-100 (Sigma) for up to 1 hour at room temperature (RT ...
-
bioRxiv - Genetics 2020Quote: Total RNA from 7 DAP spikes was extracted using TRI Reagent and 1-bromo-3-chloropropane (BCP) (Sigma-Aldrich) according to the manufacturer’s protocol ...
-
bioRxiv - Immunology 2019Quote: ... The T cells were placed above 500 μl conditioned media supplemented with 3-5 μg/ml ConA (Sigma Aldrich) in 24-well plates ...
-
bioRxiv - Biochemistry 2020Quote: ... The eluted fraction containing purified bacteriocin was concentrated to 4.6 mg/mL in an Amicon centrifugal filter concentrator with a 3 kDa cutoff membrane (Millipore) in buffer comprising 20 mM sodium phosphate (pH 6 ...
-
bioRxiv - Cell Biology 2020Quote: ... The samples were washed 3 times with 0.1 M phosphate buffer and incubated with filtered thiocarbohydrazide (TCH, Sigma, 223220) for 45 mins at 40 °C ...
-
bioRxiv - Neuroscience 2020Quote: ... Electrodes were coated with 1,1′-dioctadecyl-3,3,3′,3′-tetramethyl-indocarbocyanine perchlorate fluorescent dye (DiI, Sigma; (DiCarlo et al., 1996)) ...
-
bioRxiv - Neuroscience 2020Quote: ... Slides were washed in PBST 3 times for 10 minutes and mounted using MOWIOL-DABCO Mounting Media (Sigma, #10891) and then dried at room temperature overnight in a dark box ...
-
bioRxiv - Cancer Biology 2020Quote: ... the cells were cultured for 3 days in the presence of 3μg/ml Blasticidin S (Sigma, stock 2.5mg/ml) for selection and then serially diluted to generate single cell clones ...
-
bioRxiv - Immunology 2019Quote: ... Cell pellets were lysed in Qiagen “Phosphoprotein lysis buffer” containing 0.25% (v/v) CHAPS with 1% (v/v) phosphatase inhibitor cocktail 3 (Sigma), protease inhibitor tablet (Qiagen ...
-
bioRxiv - Cancer Biology 2020Quote: CM (without BSA) were concentrated using 3 kDa molecular weight cut-off (MWCO) Amicon ultracentrifugal units (Millipore, Burlington, MA) and lyophilized overnight ...
-
bioRxiv - Developmental Biology 2019Quote: ... short-hairpin RNA against PRKCI target sequence 5’-AGTACTGTTGGTTCGATTAAACTCGAGTTTAATCGAACCAACAGTACT-3’ was used to generate lentiviral particles (Sigma Mission-TRCN0000219727). Lentiviral particles were generated by transfecting HEK293T cells ...
-
bioRxiv - Developmental Biology 2019Quote: ... embryos in the chorion were embedded into 1% low-melting agarose with optional 0.016% Ethyl 3-aminobenzoate methanesulfonate salt (Tricaine, Cat#A5040; Sigma) in E3 embryo medium ...
-
bioRxiv - Developmental Biology 2019Quote: ... This was followed by 3 washes in PBS and a 10-minute permeabilisation in 0.25% Triton X-100 (Sigma) in PBS ...
-
bioRxiv - Biochemistry 2020Quote: ... Blots were washed 3 times for 5 min and incubated with anti-mouse-HRP antibody (0.2 μg/ml – Millipore) for 1 h in TBST ...
-
bioRxiv - Biophysics 2019Quote: ... Fractions containing the protein were pooled and concentrated by centrifugal concentrators with 3 kDa molecular weight cutoff (EMD Millipore). The sample was polished by gel filtration (120 ml Superdex200 ...
-
bioRxiv - Bioengineering 2019Quote: ... the protein solution was sterilized by passage through 0.22 μm filter and concentrated to 3 mg/mL by ultrafiltration (Amicon Ultra, Millipore) before preparation of foam matrices according to Widhe et al [14] ...
-
bioRxiv - Developmental Biology 2019Quote: ... all skate embryos and adults were euthanized with an overdose of ethyl 3-aminobenzoate methanesulfonate salt (MS-222 - Sigma) in seawater (1g/L MS-222 buffered with 2g/L sodium bicarbonate) ...
-
bioRxiv - Bioengineering 2019Quote: ... AGMS-3 cells were cultured in alpha-MEM (Thermo Fischer Scientific) containing 15% fetal bovine serum (F7524, Sigma-Aldrich), 100 U mL−1 penicillin ...
-
bioRxiv - Cell Biology 2019Quote: ... Cells were cultured as a monolayer in Dulbecco’s modified Eagle medium (DMEM, Sigma-Aldrich, USA; for SKOV-3 cells) and Ham’s F-10 Nutrient Mix (F-10 ...
-
bioRxiv - Genetics 2021Quote: ... a mouse anti-glyceraldehyde-3-phosphate dehydrogenase monoclonal antibody (#MAB374, used at 1:10,000 for the Western blot analyses, Merck Millipore); a rabbit anti-LC3B polyclonal antibody (#2775 ...
-
bioRxiv - Evolutionary Biology 2021Quote: ... Samples were derivatized in RT for 3 min with 10 μl of 60% p-toluenesulfonyl isocyanate (PTSI) (Sigma Aldrich) in ACN (Hu et al. ...
-
bioRxiv - Genetics 2021Quote: ... 2-3 eyes from different zebrafish were sonicated in RIPA lysis buffer containing protease inhibitor cocktail (Sigma, Cat# P2714). Protein samples were mixed with loading buffer and boiled for 5 minutes at 95-100°C ...
-
bioRxiv - Physiology 2021Quote: ... 100 µl homogenate were added to 400 µl of ice-cold methanol/water 4:1 (v/v) / 0.2% formic acid and isotope-labeled internal standards (10 µl 2 µM 13C3,15N-3-hydroxykynurenine (Sigma-Aldrich) and 10 µl 2 µM D5-kynurenic acid (Eurisotop)) ...
-
bioRxiv - Molecular Biology 2020Quote: ... blocked with 5% non-fat dry milk in TBS-0.1% Tween-20 for 1 h and incubated overnight with mouse anti-ataxin-3 (1:1000; Millipore), mouse anti-HA (1:1000 ...
-
bioRxiv - Plant Biology 2020Quote: ... The bottom of the tilted plates was filled with 13 ml of 0.5 M glucose solution for glucose supplementation experiments or 100 μM 3-(3,4-dichlorophenyl)-1,1-dimethylurea (DCMU, Sigma®) for sugar starvation experiments ...
-
bioRxiv - Animal Behavior and Cognition 2021Quote: ... Cre reverse primer 5’-GGT TTC CCG CAG AAC CTG AA-3’ (both primers were purchased from Sigma-Aldrich).
-
bioRxiv - Microbiology 2021Quote: ... The strain was cultured for 6 hours in BHI with 0.2% Tween 80 and washed 3 times with M9 minimal medium (11.28 g/L M9 salts (Sigma-Aldrich), 0.1 mM CaCl2 ...
-
bioRxiv - Cell Biology 2020Quote: ... permeabilized with 0.1% Triton X-100 in PBS for 3 min and blocked in PBG (PBS, 0.045% cold fish gelatin (Sigma), and 0.5% BSA) ...
-
bioRxiv - Developmental Biology 2021Quote: Embryos for imaging were anesthetized with egg water containing 0.016% tricaine (3-amino benzoic acid ethyl ester, Sigma-Aldrich), and then embedded in 1% low melting point agarose (Invitrogen 16520050) ...
-
bioRxiv - Biochemistry 2021Quote: ... adjusted to pH 5.8 using 3 M HCl and filtered with 0.2 μm nylon membrane filters (GNWP04700, 0.2 μm pore size, Merck Millipore Ltd.), while mobile phase B consisted of 100% methanol ...
-
bioRxiv - Biochemistry 2021Quote: ... adjusted to pH 8 using 3 M HCl and filtered with 0.2 μm nylon membrane filters (GNWP04700, 0.2 μm pore size, Merck Millipore Ltd.), while mobile phase B consisted of 100% methanol (method described in Table 2) ...