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Citations for Millipore Sigma :
7651 - 7700 of 10000+ citations for QuantiChrom β N Acetylglucosaminidase Assay Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2024Quote: ... the apical surface was washed and transwells were inverted into fresh 24 well culture plates containing HBSS alone or HBSS containing 100 nM N-formyl-methionyl-leucyl-phenylalanine (fMLP; Sigma; uninfected positive control). Neutrophils were applied to the basolateral compartment and transmigration was allowed to proceed for 2h (H292 cells ...
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bioRxiv - Neuroscience 2024Quote: ... mice were intra-amygdalally (i.a.) injected (ventral [V], −2.0 mm below the guide cannula) with either kainic acid (i.a.KA, Sigma-Aldrich, USA; 0.3 µg/2µL; n=30) to induce status epilepticus or sterile phosphate-buffered saline (i.a.PBS ...
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bioRxiv - Microbiology 2022Quote: ... or manual extraction kits (Invitrogen PureLink genomic DNA kit or the Sigma GenElute kit). Normalised DNA (at a concentration of 0.2 ng/ml ...
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bioRxiv - Cell Biology 2020Quote: ... and adapted to 2i condition by growing them in gelatin-coated flasks in N2B27 medium (50% neurobasal medium [Gibco], 50% DMEM/F12 [Gibco], 2 mM L-glutamine [Gibco], 0.1 mM β-mercaptoethanol, NDiff Neuro-2 supplement [Millipore], B27 serum-free supplement [Gibco]) supplemented with 1,000 units/mL LIF ...
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bioRxiv - Cell Biology 2020Quote: ... were subsequently washed three times in kinase buffer (25 mM Tris-HCl pH 7.4, 100 mM NaCl, 10 mM MgCl2, 50 μg/ml bovine serum albumin [Sigma], 0/1mM EGTA, 0.1% β-mercaptoethanol). Beads (10 μl ...
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bioRxiv - Genomics 2020Quote: ... RRID:AB_2798177) overnight at 4°C or 1:10,000 dilution of monoclonal mouse anti-human β-actin (clone AC-74, Sigma-Aldrich, Cat. No. A2228, RRID:AB_476997) for 1 hour at room temperature ...
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bioRxiv - Biochemistry 2020Quote: ... Protein expression was induced by adding 1 mM isopropyl β-D-1-thiogalactopyranoside (AppliChem GmbH, Germany) (and 135 μg/ml biotin (Sigma-Aldrich, St Louis, MI) for Sd expression) ...
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bioRxiv - Developmental Biology 2020Quote: ... Gentle pressure was applied to epididymis tissue using forceps and sperm were allowed to swim out during a 45-minute incubation in HTF + 0.75mM methyl-β-cyclodextrin (Millipore MR-070-D, Sigma C4555) at 37°C ...
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bioRxiv - Cell Biology 2019Quote: ... (Piperno and Fuller 1985)), mouse-anti-β-tubulin (E7; at 1:100; Developmental Hybridoma Studies Bank, University of Iowa) and rabbit-anti-Zwf1 (Sigma A9521; at 1:10,000), followed by goat-anti-mouse-680 (LI-COR 926-68070 ...
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bioRxiv - Immunology 2021Quote: ... [AppliChem, Cat. #A3452], 25 mM MgCl2 [Sigma, Cat. #M2670], 450 mM KCl [AppliChem, Cat. #A293], 0.8 mM β-NAD [Sigma, Cat. N1511], 60 mM (NH4)2SO4 [Fisher Scientific ...
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bioRxiv - Microbiology 2020Quote: ... 100,000 x g (membrane fraction) centrifugations were suspended in 100 μL of 2X loading buffer (4% SDS, 10% β-mercaptoethanol, Sigma-Aldrich, 20% (v/v) glycerol ...
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bioRxiv - Microbiology 2020Quote: ... 1:2000 (SARS-1-S, MERS-S and SARS-2-S) or 1:5000 (229E-S)] and mouse anti-β-actin (Sigma-Aldrich, A5447, 1:5000). As secondary antibody ...
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bioRxiv - Developmental Biology 2020Quote: ... The blot was then stripped and re-probed with a 1:5000 dilution of anti-β-Actin antibody (Sigma catalogue #A2066, St. Louis, MO, USA) in TBST with 1% milk ...
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bioRxiv - Neuroscience 2019Quote: The following drugs were used for behavioral pharmacology experiments: the non-selective β-adrenergic receptor (βAR) antagonist DL-propranolol hydrochloride (Sigma-Aldrich, St. Louis, MO), the α1AR antagonist prazosin hydrochloride (Sigma-Aldrich) ...
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bioRxiv - Cell Biology 2021Quote: ... yeast cells were pre-cultured for 24h in SD–URA at 30°C and then shifted to SD-LEU medium supplemented with 1 µM β-Estradiol (Sigma- Aldrich, St. Louis, MO), incubated for 16-18h at room temperature ...
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bioRxiv - Immunology 2022Quote: ... membranes were incubated with PBS-T or TBS-T complemented with 5 % BSA and primary antibodies specific for β-actin (Sigma-Aldrich, A5441, 1:2000), calnexin (Enzo Life science ...
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bioRxiv - Immunology 2022Quote: The primary and secondary antibodies that were used throughout our studies include the following: mouse anti-β-tubulin (1:5000; catalog # T8328-200ul, Sigma-Aldrich, St. Louis, MO), mouse anti-GAPDH (1:20,000 ...
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bioRxiv - Plant Biology 2022Quote: ... homogenized in extraction buffer A (10mM Tris-HCl, 0.4M sucrose, 10mM MgCl2, 2mM PMSF, 5mM β-mercaptoethanol, 1µg/ml protease inhibitor (Sigma-Aldrich, catalog No. 200-664-3), incubated with gentle shaking for 20min ...
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bioRxiv - Immunology 2023Quote: ... we removed the head kidneys from RNAlater and placed them in a tube with ∼10 ceramic beads (1.4 mm) and 600 µL of RTL Buffer enriched with 6 µL β-mercaptoethanol (Sigma-Aldrich, St. Louis, MO, USA) to avoid sample degradation by RNases ...
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bioRxiv - Developmental Biology 2024Quote: ... For each IVF session one epididymis from 12-week-old WT and one from Trim66-null were transferred into 90 μL of capacitation medium, consisting of TYH (Takeo & Nakagata, 2011) with 0.75 mM methyl-β-cyclodextrin (MBCD, Sigma-Aldrich, Merck KGaA, Darmstadt, Germany). Spermatozoa were allowed to disperse from the tissue and incubated for 30 min in a 5% CO2 incubator at 37°C.
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bioRxiv - Plant Biology 2024Quote: ... Kit (Sigma). RNA was treated with TURBO DNAse (Thermo ...
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bioRxiv - Cell Biology 2020Quote: ... or cellulose solution in N-hydroxysuccinimide (NHS; Aldrich; 100 mM) and 1-ethyl-3-(3-dimethylaminopropyl)carbodiimide (EDC, Sigma; 100 mM, 90 min, RT)44 ...
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bioRxiv - Physiology 2022Quote: ... cells were washed two times with PBS+/+ and medium was replaced with glucose-free DMEM (catalog n°D5030, Sigma-Aldrich, Saint-Louis, MO, USA) without phenol red supplemented with 2 mM L-glutamine ...
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bioRxiv - Plant Biology 2019Quote: ... and axr3-3 were expressed as 6X His-tag (N-terminal) fusion proteins in Escherichia coli strain Rosetta™ DE3 competent cells (Supplementary Table 4; Novagen, product code: 70954). These proteins were expressed in minimal media with 13C D-glucose and 15N ammonium chloride ...
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bioRxiv - Animal Behavior and Cognition 2020Quote: ... the mothers of the experimental animals returned to their home cages and were treated daily with a stainless steel feeding needle per oral gavage with either fluoxetine (10 mg/kg, n= 6 dams) or vehicle (Methylcellulose 1%, (Sigma, St. Louis, MO, USA), n=4 dams ...
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bioRxiv - Microbiology 2020Quote: ... and kept for 60 s at pH 7.4.The mixture was digested with N-tosyl-L-phenylalanyl chloromethyl ketone (TPCK)-treated trypsin (Sigma-Aldrich, St. Louis, MO, USA) at a concentration of 200 μg/mL in the presence of 1% Triton X-100 ...
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bioRxiv - Microbiology 2019Quote: ... C57BL/6 and Trem-2 KO male mice with 10 weeks of age were fasted for 15 hours prior to intra-peritoneal injection with 300mg/Kg of acetaminophen (N-acetyl-p-aminophenol (APAP)) (Sigma, St. Louis, MO, USA) in PBS or PBS only ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... a sub-set of benzene and air-exposed mice (n=24/group) were treated with 0.5 mg/kg lipopolysaccharides (LPS, Sigma Cat# 2630, Lot# 028M4022V; i.p). At the end of the exposure protocol ...
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bioRxiv - Neuroscience 2020Quote: ... The cleared hemispheres were then transferred to CUBIC-RA solution (45% w/w antipyrine, 30%w/w N-methylnicotinamide, in H2O, Sigma Aldrich, refractive index=1.5) and again kept at 37°C under continuous shaking ...
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bioRxiv - Biochemistry 2021Quote: ... All standard amino acids were purchased from AAPPTec except for: N-α-Fmoc-O-t-butyl-L-trans-4-hydroxyproline (Hyp) was purchased from EMD Millipore (Billerica, MA), Fmoc-γ-carboxy-glutamic acid γ,γ-di-t-butyl ester (Gla ...
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bioRxiv - Developmental Biology 2021Quote: 30 embryos laid within a day were transferred to a standard food supplemented with 0.1 mg/ml N-acetyl cysteine(NAC) (Sigma-Aldrich(Saint Louis, MO, USA),#A7250 ...
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bioRxiv - Microbiology 2020Quote: ... thirty mice with colitis were obtained and randomly assigned into three groups (n =8) including the control group (Control) without EA (EA, Sigma-Aldrich,CAS141-43-5) supplements and two treatment groups that were supplemented with 500μM EA (LowEA ...
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bioRxiv - Neuroscience 2022Quote: ... and cervical spinal cord tissues (C3-C6) were isolated (n=5-8/treatment; 1 male and 1 female per litter) and sonicated in Tri-Reagent (Sigma, St. Louis, MO, USA) and stored at −80°C ...
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bioRxiv - Cancer Biology 2022Quote: ... Two weeks following infection, the drinking water provided to hamsters was supplemented with dimethylnitrosamine (DMN) (synonym, N-nitrosodimethylamine) (Sigma-Aldrich, Inc., St. Louis, MO) at 12.5 ppm until 10 weeks following infection (7 ...
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bioRxiv - Cancer Biology 2024Quote: ... six-to eight-week-old ΔSPC and wild type FVB/N mice were intraperitoneally (i.p.) injected with urethane (1 mg/g body weight, Sigma-Aldrich, St. Louis, MO, USA) once a week for six consecutive weeks [6] ...
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bioRxiv - Molecular Biology 2023Quote: ... The anti-AGO1-N-coil antibody was detected with anti-Rabbit antibody conjugated to HRP (1:2,000 [Sigma] in 5% milk in PBS-T). The intensity of the signal from retained anti-AGO1-N-coil antibody was quantified using Image J and fitted using Nonlinear Least Square (NLS ...
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bioRxiv - Microbiology 2023Quote: ... #M-3258), TMAO (Trimethylamine N-oxide, #317594), NaClO (Sodium hypochlorite #425044), and BV (Benzyl Viologen, #B8133) were all purchased from Sigma-Aldrich (Saint Louis, MO).
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bioRxiv - Bioengineering 2023Quote: ... poly[di(carboxyphenoxy)phosphazene] disodium salt (PCPP, 1 MDa), spermine tetrahydrochloride, calcium chloride dihydrate (CaCl2), and sodium hydroxide solution (NaOH, 1 N) were purchased from Sigma-Aldrich (St. Louis, MO). Anhydrous sodium sulfide (Na2S ...
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bioRxiv - Bioengineering 2023Quote: ... 850365C) and 1,2-distearoyl-sn-glycero-3-phosphoethanolamine-N-[methoxy(polyethylene glycol)-2000] (ammonium salt) (DSPE-PEG2K; 880129C) (Sigma Aldrich, St Louis, MO, USA) lipids were combined in a molar ratio of 90:10 and prepared using the thin film hydration method ...
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bioRxiv - Cell Biology 2023Quote: RNA was individually extracted from 10 larvae each group (control and hypergravity) for one biological replicate (N = 20 total) with TRIzol (T9424, Sigma-Aldrich, St. Louis, Missouri) according to manufactured procedures ...
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bioRxiv - Cell Biology 2024Quote: ... Phase separation of lipids from the serum was performed using an organic phase of diisopropyl ether: n-butanol (3:2; Sigma Aldrich, 296856, B7906, respectively). Organic and aqueous phases were allowed to mix at room temperature with continuous stirring in the dark for 1 h ...
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bioRxiv - Bioengineering 2024Quote: ... 1,2-dipalmitoyl-sn-glycero-3-phosphoethanolamine-N-[methoxy(polyethylene glycol)-5000] (DPPE-PEG5K) and chloroform were purchased from Sigma-Aldrich (St. Louis, MO, USA). Phosphate-buffered saline was procured from Fisher Scientific (Pittsburg ...
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bioRxiv - Molecular Biology 2024Quote: ... cells were pipetted onto a pad of 2% agarose prepared in YES media with 0.1 mM N-propyl gallate (Sigma-Aldrich, catalog no. P-3130) and 1% gelatin (Millipore ...
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Cellular sialoglycans are differentially required for endosomal and cell-surface entry of SARS-CoV-2bioRxiv - Microbiology 2024Quote: ... Cells were then fixed using 10% formalin and processed for immunofluorescence using an antibody against the HCoV-OC43 N protein (Millipore #MAB9013; diluted 1:500) and a secondary Alexa Fluor 555 conjugated anti-mouse IgG antibody (NEB #4413S ...
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bioRxiv - Cell Biology 2020Quote: Proximity ligation assay was performed according to manufacturer’s protocol (Duolink™ PLA Technology, Sigma). Briefly ...
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bioRxiv - Cancer Biology 2021Quote: Transwell assays were performed in 24-well PET inserts (Millipore, 8.0-μm pore size) for cell migration ...
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bioRxiv - Molecular Biology 2020Quote: Proximity ligation assay (PLA) was performed using the Duolink in situ-fluorescence (Sigma DUO92101). For G9a and Sp1 interaction ...
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bioRxiv - Cell Biology 2020Quote: ... Protein lysates were prepared using radioimmunoprecipitation assay (RIPA) buffer (Sigma Aldrich, St Louis, MO) as per manufacturer’s guidelines ...
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bioRxiv - Microbiology 2021Quote: ... Assays were carried out in black Nunc flat-bottomed microtitre plates (Sigma-Aldrich, #P8741), and technical triplicates were included for each sample ...
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bioRxiv - Evolutionary Biology 2021Quote: The assays were performed in 96 well microplates in M9 minimal media (Sigma-Aldrich) supplemented with MgSO4 ...