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Citations for Millipore Sigma :
7201 - 7250 of 10000+ citations for Anti IL 4I1 Antibody since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2023Quote: ... Membranes were blocked with 10% skim milk (in 1x TBS-T) for 1 h and probed with anti-GFP antibody (1:1,000; Sigma-Aldrich) in 5 % skim milk (in 1x TBS-T ...
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bioRxiv - Biophysics 2023Quote: ... Samples were then transferred to a PVDF membrane and analyzed by Western blot using a mouse-anti-myc primary antibody (Sigma) and HRP-coupled goat-anti-mouse secondary antibody (Jackson ImmunoResearch) ...
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Neural mechanisms underlying uninstructed orofacial movements during reward-based learning behaviorsbioRxiv - Neuroscience 2023Quote: ... the slices were washed three times with a blocking buffer containing 1% bovine serum albumin and 0.25% Triton-X in phosphate buffer saline (PBS) and then incubated with primary antibodies (anti-tyrosine hydroxylase, rabbit polyclonal, 1:1000, Merck Millipore; anti-D1R ...
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bioRxiv - Molecular Biology 2023Quote: ... The process was the same as in fibroblasts and the primary antibodies were anti-human NANOG (1:100, Millipore #AB5731) and SSEA4 (1:25 ...
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bioRxiv - Neuroscience 2023Quote: ... Slides were then washed in PBS and incubated with rabbit anti-TH primary antibody (1:200, Millipore AB152, RRID: AB_390204) overnight at 4°C ...
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bioRxiv - Molecular Biology 2023Quote: ... The primary antibodies used for all western blot (WB) experiments and their dilutions were as follows: Anti-FLAG (Sigma, #F3165) at 1:5000 dilution ...
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bioRxiv - Neuroscience 2023Quote: ... the samples were incubated overnight at 4 °C with a rat monoclonal anti-poly(GR) antibody (clone 5A2, MABN778, Millipore), a mouse monoclonal anti-poly(GA ...
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bioRxiv - Cell Biology 2023Quote: ... cells were washed three times with PBS and stained with anti-rabbit IgG antibody conjugated to Alexa488 (Sigma- Aldrich, SAB4600045) and anti-mouse IgG antibody conjugated to Alexa647 (Thermo Fischer Scientific ...
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bioRxiv - Biochemistry 2023Quote: ... The proteins were resolved in SDS-PAGE gels and transferred to nitrocellulose membranes that were incubated overnight at 4 °C with one of the following antibodies: mouse monoclonal anti-hemagglutinin (1:2000, Sigma); rabbit anti-P-TrkB-Tyr674/675 (1:1000 ...
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ANGPTL8 R59W variant influences inflammation through modulating NF-κB pathway under TNFα stimulationbioRxiv - Biochemistry 2023Quote: ... GAPDH was used as internal control for protein loading and detected using anti-GAPDH antibody (ab2302; Millipore, Temecula, CA, USA).
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bioRxiv - Plant Biology 2023Quote: ... Flag-ELF7 and GFP-VIP3 ChIP-seqs were performed using 7-day-old seedlings grown under standard conditions and an anti-Flag M2 antibody (F1804, Sigma) and an anti-GFP antibody (ab290 ...
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bioRxiv - Molecular Biology 2023Quote: ... Immunoprecipitation of the crosslinked UNK-RNA or PABPC1-RNA complexes was carried out using anti-Flag antibody (Millipore Sigma, F1804) or anti-PABPC1 antibody (Abcam ...
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bioRxiv - Bioengineering 2023Quote: ... and incubated for 4 hours with an anti-rabbit secondary antibody coupled with Rhodamine (#SAB3700846 Sigma-Aldrich, dilution 1:200). Cells were incubated in AlexaFluor 488 phalloidin (#R37110 ...
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bioRxiv - Biochemistry 2023Quote: ... the plates were incubated with 100 μL/well horseradish peroxidase-conjugated anti-mouse immunoglobulin second antibody (Sigma, St. Louis, MO) in Buffer D of pCa 9 or 4 at room temperature for 1 h ...
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bioRxiv - Developmental Biology 2023Quote: ... Gradual conversion of each sample to 1XPBT was performed using a diluted series of SSC/PBT and samples were blocked in blocking buffer for 3 hours at room temperature before adding 1:10000 of anti-DIG antibody (Millipore/Sigma) overnight at 4°C ...
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bioRxiv - Neuroscience 2022Quote: ... Dig epitopes were detected with alkaline phosphatase-coupled anti-Dig fab fragments antibody at 1:2500 (Millipore Sigma, cat. #11093274910), and the signal revealed with Nitro Blue Tetrazolium chloride/5-Bromo-4-Chloro-3-Indolyl Phosphate ...
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bioRxiv - Genetics 2023Quote: ... Then the cells were incubated for 1 hour at room temperature with 1:500 diluted anti-Cas9 primary antibody (Sigma, SAB4200701-25UL ...
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bioRxiv - Developmental Biology 2023Quote: ... The samples were incubated overnight in a humid chamber at 4°C with the following primary antibodies: mouse anti-pan polyglycylated tubulin (1:1000, Axo-49; Sigma) and anti-cleaved caspase 3 (1:500 ...
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bioRxiv - Microbiology 2023Quote: Protein extract from leaf tissue was incubated for 2h at 4°C with an anti-flag mouse antibody at 1:4,000 dilution (Sigma, F1804), followed by a 1h-incubation at 4°C with PureProteomeProtein A/G Magnetic Beads Mix (Millipore) ...
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bioRxiv - Plant Biology 2022Quote: ... 3-6 µg total protein was derivatized with DNPH and subsequently detected with an anti-DNP antibody according to the manufacturer’s instruction of the OxyBlot Protein Oxidation Detection Kit (S7150, Sigma-Aldrich). Equal amounts of the derivatized protein samples were employed as loading control on a separate SDS-gel and stained with Coomassie or silver staining.
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bioRxiv - Biochemistry 2022Quote: ... FLAG-tagged L protein mutants were detected using horseradish peroxidase-conjugated anti-FLAG M2 antibody (1:9,000) (A8592; Sigma-Aldrich) and bands were visualised by chemiluminescence using Clarity Max Western ECL Substrate (Bio-Rad ...
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Phosphorylation controls spatial and temporal activities of motor-PRC1 complexes to complete mitosisbioRxiv - Biochemistry 2023Quote: ... Immunofluorescence in human cells was conducted as previously described using antibodies against tubulin (1:1000 anti-beta tubulin, mouse, T7816, Sigma OR 1:2000/1:1000 anti-alpha tubulin ...
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bioRxiv - Synthetic Biology 2022Quote: ... Grids were blocked in 2% BSA in TBST for 30 minutes and then moved into a 20 µl drop of anti-His tag primary antibody (Sigma) diluted 1:100 ...
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bioRxiv - Neuroscience 2023Quote: ... for 1 hr at room temperature (RT) and incubated with mouse monoclonal anti-aggrecan neoepitope antibody (10 µg/ml; clone AF-28, MAB19310-C, Millipore) and rabbit polyclonal anti-α-tubulin antibody (2 µg/ml ...
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bioRxiv - Plant Biology 2023Quote: ... Membranes were washed and further incubated with secondary antibody (goat anti-mouse AP, 1:15000; Sigma Aldrich, St. Louis, MO). The membrane was washed with TBS-Tween 20 (TBS-T ...
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bioRxiv - Microbiology 2023Quote: ... Ultrathin sections of 50 nm were blocked with 5% fetal bovine serum/5% normal goat serum for 30 min and subsequently incubated with rabbit anti-HA antibody (Sigma) for 60 min at room temperature ...
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bioRxiv - Neuroscience 2023Quote: The primary antibodies used were: a rabbit polyclonal anti-GFP in a 1:400 dilution (Millipore Sigma, AB3080, lot: 3717722), a mouse monoclonal anti-NeuN in a 1:500 dilution that is a marker for mature neurons (Millipore Sigma ...
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bioRxiv - Neuroscience 2024Quote: ... Sections were then incubated for 48 h in the primary antibodies: Monoclonal mouse anti-parvalbumin (1:5000; P3088; Sigma-Aldrich) and Wisteria floribunda lectin (1:500 ...
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bioRxiv - Cell Biology 2024Quote: ... Following secondary antibodies at the indicated dilution were used for IF: Anti-rabbit IgG (Fc) TRITC (Sigma #SAB3700846, 1:500), Anti-mouse IgG (Fc ...
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bioRxiv - Developmental Biology 2024Quote: ... Embryos were then washed briefly and incubated at 37°C for 30 min in 20% anti-mouse serum antibody (Sigma) or 20% anti-human serum antibody (Sigma) ...
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bioRxiv - Neuroscience 2024Quote: ... then incubated overnight with the following primary antibodies diluted in block: Anti-myelin basic protein (MBP, 1:100, Millipore #MAB386), anti-NeuN (1:500 ...
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bioRxiv - Cell Biology 2024Quote: The following antibodies were used for immunohistochemistry and western blots: mouse anti-tubulin clone B-5-1-2(Millipore Sigma), rabbit anti-CAPS (Abcam EPR15631) ...
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bioRxiv - Microbiology 2023Quote: ... incubated for 1 h at room temperature with an anti-mouse IgG secondary antibody conjugated with peroxidase (Sigma Aldrich, A9044) diluted 1:10,000 in blocking buffer ...
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bioRxiv - Microbiology 2023Quote: ... Protein samples resolved by polyacrylamide gel electrophoresis were transferred to 0.2 μm nitrocellulose membranes and probed with either anti-FLAG M2 monoclonal antibodies (Sigma-Aldrich), used at a dilution of 1:10,000) ...
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bioRxiv - Molecular Biology 2023Quote: ... isolated macular retinal nuclei were stained with a mouse anti-NeuN monoclonal antibody (1:5000, Alexa Flour 488 Conjugate MAB377X, Millipore, Billerica ...
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bioRxiv - Molecular Biology 2023Quote: ... the localization was made visible by immunofluorescence staining using an anti-flag antibody (F7425, Sigma Aldrich, Merck, Kenilworth, NJ, USA) in combination with Alexa 546 secondary antibody (Invitrogen ...
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bioRxiv - Microbiology 2023Quote: ... This was followed by four washes with 200 μl of incubation buffer and incubation with 50 μl of anti-His-HRP antibody (Sigma), diluted 1:2000 in incubation buffer for 1 hr at room temperature ...
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bioRxiv - Molecular Biology 2023Quote: ... CRHR2α expression levels in the stably transfected cell lines was assessed by Western blot using a mouse monoclonal anti FLAG -antibody (1:5000 dilution; SIGMA). All the experiments were performed at least 3 times with 3 individual clones (clones 4 ...
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bioRxiv - Molecular Biology 2023Quote: ... The presence of purified Dr20/22 was confirmed by SDS-PAGE and Western blotting using the Anti-polyHistidine Peroxidase antibody (Sigma). The occurrence of potential glycosylation sites was assessed using the Glycoprotein Staining Kit (Pierce).
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bioRxiv - Bioengineering 2023Quote: ... The membrane was blocked for 1 h in 5% milk in PBS-T then incubated overnight with an anti-flag antibody (Sigma) or actin antibody (Sigma ...
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bioRxiv - Cell Biology 2023Quote: ... 20 μl of clear lysates and debris were loaded onto the same 10% Bis-Tris gel for the comparison of total protein (Coomassie staining) and ubiquitinated protein levels (anti-ubiquitin antibody, Sigma, 05-944 ...
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bioRxiv - Cell Biology 2023Quote: ... cells were incubated for 1h at room temperature with primary antibodies diluted in 2% fatty acid free BSA: anti-FLAG (1:1,000; Clone M2, Sigma, F1804), anti-Rubicon (1:500 ...
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bioRxiv - Immunology 2023Quote: ... were isolated from PBMCs using anti-CD14-antibody-coated magnetic beads (Miltenyi) and cultured in RPMI1640 supplemented with 10% human AB serum (Sigma), pen/strep ...
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bioRxiv - Genomics 2023Quote: ... then stained with anti-NeuN antibody (directly conjugated to Alexa Fluor 488; Millipore cat. No. MAB377X, clone A60; 1:1,250) and an anti-rabbit IgG Alexa Fluor 647 antibody as a negative control for 30min ...
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bioRxiv - Neuroscience 2023Quote: ... diluted in PBS for 1 h before immunoreaction with the following antibodies: mouse anti-GFAP (1:1,000; Millipore, Cat. MAB360), rabbit anti-GFAP (1:1,000 ...
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bioRxiv - Bioengineering 2023Quote: ... sections were incubated at 4 °C overnight with a 1:100 diluted mouse monoclonal anti-type I collagen antibody (C2456; Sigma). For Col-II staining ...
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bioRxiv - Cell Biology 2023Quote: ... Hsp60 (1:1000, QED-BIOSCIENCE 11101) or glyceraldehyde-6-phosphate dehydrogenase (rabbit polyclonal anti-G6PDH antibody, 1:10000, Sigma A9521). Bound antibodies were detected using the appropriate horseradish peroxidase-conjugated secondary antibodies (1:10000 ...
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bioRxiv - Cancer Biology 2023Quote: ... 2 µg of the fragmented total RNA per sample was used for RNA immunoprecipitation (IP) with 5 µg of the anti-m6A antibody (ABE572, Millipore). RNA-seq libraries from input and IP samples were prepared using the SMARTer Pico Input Mammalian v2 RNA-seq kit (Takara ...
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bioRxiv - Cell Biology 2023Quote: ... the cells were incubated with a 1:200 dilution of the following antibodies overnight at 4°C: anti-trimethyl-Histone H3 (Lys27) (07-449, Millipore), anti-trimethyl-Histone H3 (Lys9 ...
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bioRxiv - Plant Biology 2023Quote: ... Membranes were blocked using 5% nonfat milk and probed with anti-poly-His primary antibody (cat. no. H1029; Sigma-Aldrich) at 1:3,000 dilution or anti- GST primary antibody (cat ...