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Citations for Millipore Sigma :
7401 - 7450 of 10000+ citations for Anti IL 4I1 Antibody since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2021Quote: ... and stained with sACE2-humanFc fusion protein (construct is a gift of Jason McLellan, University of Texas at Austin) or mouse anti-Flag antibody (Sigma, F3165). Cells were then stained with anti-human-IgG-FITC (Sigma ...
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bioRxiv - Neuroscience 2020Quote: ... in Triton PBS containing 1% v/v NDS and a mixture of between two and four of the following primary antibodies: goat anti-choline acetyltransferase (ChAT; 1:500 dilution; AB144P, Millipore, RRID:AB_2079751); goat anti-forkhead box protein P2 (FoxP2 ...
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bioRxiv - Microbiology 2021Quote: ... and extensively dialysed against HEPES-buffered saline and their level of expression determined by ELISA using a mouse monoclonal anti-His antibody (His-Tag mAb, EMD Millipore) as primary antibody and a goat anti-mouse alkaline phosphatase-conjugated secondary (Bethyl).
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bioRxiv - Neuroscience 2019Quote: ... brain slices were blocked in PBS-BTA (4% BSA+ 0.25% TritonX100+ 0.02% sodium azide prepared in 1XPBS) for 1 hr followed by incubation with primary antibody-anti-Parvalbumin (1:1000, P3088 Sigma-Aldrich) at RT for 4 hrs with gentle shaking ...
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bioRxiv - Physiology 2021Quote: ... Sections were subsequently incubated with the following primary antibodies for 1 h at room temperature: polyclonal rabbit anti-laminin IgG (L9393, 1:700; Sigma-Aldrich), monoclonal mouse anti-MHCI IgG2b (BA-F8 ...
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bioRxiv - Molecular Biology 2021Quote: ... samples were washed and incubated for 1 h at room temperature with primary antibody (anti-HA, mouse, 1:500, Sigma-Aldrich) diluted in PBS containing 1 % bovine serum albumin ...
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bioRxiv - Molecular Biology 2021Quote: ... Expression of the dCasRX-YTHDC1 fusion protein was confirmed by transfection of the plasmid followed by Western Blot with anti-FLAG M2 mouse monoclonal antibody (F1804, Sigma-Aldrich) and anti-YTHDC1 (14392-1-AP ...
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bioRxiv - Microbiology 2020Quote: ... Coverslips were next labeled as follows: HA-tagged proteins were detected by anti-HA rat monoclonal antibody 3F10 (Sigma, 1:200), parasite cyst wall and cyst matrix by in-house SalmonE anti-CST1 antibody (1:500 ...
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bioRxiv - Genomics 2021Quote: Specific antibodies recognizing human H1 variants used for ChIP/ChIP-seq were: anti-H1.0/H5 clone 3H9 (Millipore, 05-629-I), anti-H1.2 (Abcam ...
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bioRxiv - Cell Biology 2021Quote: ... and immunoblotting was performed using the following antibodies: anti-K63 ubiquitin (1:4,000; EMD Millipore, cat. no. 05-1308, clone apu3), anti-actin (1:6,000 ...
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Role of autophagy in sepsis-induced skeletal muscle dysfunction, whole-body metabolism, and survivalbioRxiv - Cell Biology 2021Quote: ... TA muscles were carefully removed and then frozen in liquid nitrogen and stored (−80 °C) for immunoblotting analysis using a mouse IgG2a monoclonal anti-puromycin antibody (clone 12D10, 1:2500; Millipore # MABE343).
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bioRxiv - Neuroscience 2020Quote: ... Every 9th free-floating section was selected and stained with mouse anti-NeuN antibody (EMD Millipore, Billerica, MA, USA; 1:1000), which is a neuronal marker ...
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bioRxiv - Neuroscience 2020Quote: ... Blocking buffer was removed and replaced with 500 μl of a solution containing a 1:500 dilution of anti-tyrosine hydroxylase antibody (Millipore Sigma). Slices were incubated overnight with side to side rotation at 4°C ...
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bioRxiv - Biophysics 2020Quote: ... permeabilized cells were blocked with 3 % BSA in PBS for 30 min at room temperature and then incubated with mouse monoclonal anti-alpha-tubulin antibody (T6199, clone DM1A, Sigma-Aldrich) diluted at 1:200 in 3 % (w/v ...
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bioRxiv - Biophysics 2020Quote: ... Primary antibody staining was carried out by placing coverslips upside down onto a drop of primary antibody staining mix (mouse-anti-beta-tubulin, catalog no. T5293, Sigma-Aldrich, diluted 1:300 in PBS containing 2% (w/v ...
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bioRxiv - Cell Biology 2020Quote: ... membranes were washed five times with TBS and incubated with secondary antibody (goat-anti-rabbit HRP-conjugated,1:2000 in TBS + 5% dry milk, Sigma Aldrich) for one hour at RT ...
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bioRxiv - Cell Biology 2021Quote: ... sections were washed, incubated with a goat anti-rabbit 488 secondary antibody (1:250, Laboratory) and Hoechst (1:500, Sigma-Aldrich) in PBS for 45 min at RT ...
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bioRxiv - Cell Biology 2022Quote: ... and incubated o/n at 4°C with the following primary antibodies at a dilution of 1/1000: anti-LC3 (Sigma, L7543), anti-Atg5-12 (Abnova ...
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bioRxiv - Cell Biology 2022Quote: ... Puromycin-labeled proteins were detected either by Western blotting or by indirect fluorescence microscopy using a monoclonal anti-puromycin antibody (12D10; Sigma Aldrich).
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bioRxiv - Neuroscience 2022Quote: ... Then the sections were incubated with the primary antibodies (12h at 4 °C): anti-ChAT (1:500, goat, Sigma-Aldrich, AB144P). Then the sections were washed in PBS five times at room temperature ...
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bioRxiv - Neuroscience 2022Quote: ... followed by an overnight incubation at 4°C with rabbit anti-HA antibody (1:1000, Cat no. H6908, Sigma-Aldrich, USA). For c-Fos ...
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bioRxiv - Neuroscience 2022Quote: ... the membrane was incubated for 12 h at 4°C with the primary antibody: anti-VGlut1(AB5905, Millipore, Burlington, 1:2000), anti-PSD95 (51-6900 ...
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bioRxiv - Immunology 2022Quote: ... Slides were then washed in dH2O and TBS-T before incubation overnight at room temperature with a rabbit anti-CD34 antibody (HPA036723, Millipore Sigma), a mouse anti-CD20 antibody (M0755 ...
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bioRxiv - Neuroscience 2022Quote: ... Sections were incubated in PBS containing 0.5% normal donkey serum and 0.03% Triton X-100 for 1 h at room temperature (RT, 23 ± 7□) and then stained with primary antibodies overnight at 4□ (rabbit anti-c-Fos, 1:5000, Sigma-Aldrich; rat anti-CTIP2 ...
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bioRxiv - Developmental Biology 2022Quote: ... The following primary antibodies and their respective dilutions were used in western blotting (WB) and CUT&RUN experiments: anti-Tubulin (Sigma, #T6199), 1:5000 (WB) ...
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bioRxiv - Molecular Biology 2022Quote: ... The membrane was blocked with 5% non-fat milk in TBST buffer and was incubated subsequently with monoclonal ANTI-FLAG® M2 antibody (Sigma) and goat anti-mouse IgG (H+L)-HRP conjugate (Bio-rad) ...
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bioRxiv - Molecular Biology 2022Quote: ... the rest of supernatant (250 μL, IP) was transferred into another tube and incubated with anti-Flag antibody (1:100, Sigma-Aldrich) or control immunoglobulin G (IgG ...
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bioRxiv - Molecular Biology 2022Quote: ... cells were washed three times with PBS for 10 min at room temperature and incubated in primary antibody (anti-αSMA, Sigma, C6198) or anti-HuR ...
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bioRxiv - Plant Biology 2022Quote: ... 10 μL per sample was analyzed by western blotting using monoclonal anti-GFP antibodies conjugated to horseradish peroxidase (HRP; Sigma-Aldrich) and separated by 8% SDS-PAGE ...
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bioRxiv - Physiology 2022Quote: ... Cells were then incubated ON at 4°C with the following primary antibodies diluted in blocking buffer: anti-FLAG (1:200, M2 clone, catalog no. F1804, Sigma-Aldrich); anti-FLAG (1:200 ...
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bioRxiv - Physiology 2022Quote: ... Membranes was incubated with the primary antibodies against target proteins: rabbit polyclonal anti-TSG 101 (T5701, Sigma-Aldrich Co., 1:200), rabbit polyclonal anti-CD63 (SAB4301607 ...
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bioRxiv - Molecular Biology 2022Quote: ... Western blots were probed with the following antibodies at the indicated concentrations: rabbit polyclonal anti-FLAG (Millipore Sigma, F7425; 1:1,000), mouse monoclonal anti-FLAG M2 (Millipore Sigma ...
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bioRxiv - Microbiology 2022Quote: ... FLAG-tagged proteins were probed with immunoblotting using monoclonal anti-Flag M2-Peroxidase (HRP) antibody (Sigma-Aldrich, Saint Louis, MO, USA) (1/50,000X dilution ...
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bioRxiv - Microbiology 2022Quote: ... Epitope-tagged 3xFLAG proteins were detected using primary antibody anti-FLAG M2 from mouse (diluted 1:4,000, catalogue number F3165; Sigma Aldrich, Germany) and Alexa Fluor 680 fluorescent dye-labeled secondary anti-mouse antibody from goat (diluted 1:10,000 ...
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bioRxiv - Biophysics 2022Quote: ... The tyrosinated tubulin level was detected by western blot using monoclonal anti-tyrosinated tubulin antibody (clone YL1/2; 1:2500 dilution; EMD Millipore) as primary antibody and a goat anti-rat secondary antibody (alkaline phosphatase-conjugated ...
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bioRxiv - Neuroscience 2021Quote: ... sections were incubated overnight at 4°C with the primary antibodies (Abs): 1:400 rabbit anti-GFAP (EMD Millipore catalog#ABS5804); 1:1000 rabbit anti-Iba1 (Wako catalog#019-19741) ...
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bioRxiv - Neuroscience 2021Quote: ... Neural dendrites were detected with the neurofilament marker anti-SMI 311 mouse monoclonal antibody (1:600, Millipore Bioscience Research Reagents, USA). The day after ...
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bioRxiv - Neuroscience 2021Quote: ... and 0.2% Triton X-100 and then incubated overnight at 4°C with a mouse anti-tyrosine hydroxylase antibody (TH, Sigma, T1299) at 1:200 dilution in PBS containing 1.5% BSA and 0.2% Triton X-100 ...
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bioRxiv - Neuroscience 2020Quote: ... Sections were then incubated with one or more of the following primary antibodies (rabbit anti-TH, 1:2000, AB152, EMD Millipore; guinea pig anti-VGLUT2 ...
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bioRxiv - Cell Biology 2021Quote: ... the supernatants obtained were incubated with gentle rotation for 1 h at 4°C with anti-FLAG tag-antibody-conjugated magnetic beads (Sigma-Aldrich). The beads were then washed three times with lysis buffer #1 and incubated with gentle rotation for 30 min at 4°C with 50 μL of an elution buffer (50 mM HEPES-KOH ...
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bioRxiv - Neuroscience 2020Quote: ... in TBS with 0.4 % Triton X-100 and incubated with a mouse anti-GFAP antibody (1:500, clone G-A-G, Sigma: G 3893) in blocking solution overnight at 4°C ...
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bioRxiv - Neuroscience 2020Quote: ... followed by overnight incubation at 4 °C with the following primary antibodies diluted in blocking solution: mouse anti-GFAP (1:500, Sigma G3893), guinea pig anti-NeuN (1:500 ...
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bioRxiv - Cell Biology 2021Quote: ... Membranes were washed with TBS-T (3 times for 10 min) and incubated with horseradish peroxidase-conjugated secondary antibodies anti-mouse (Millipore, #AP308P), or anti-rabbit (Thermo Scientific ...
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bioRxiv - Biochemistry 2020Quote: ... 15-fold dilution of the result sample was loaded and resolved in 12% SDS-PAGE followed by immunoblot analysis anti-polyHIS monoclonal antibodies (Sigma, H1020). Scale-up experiments were done in order to purify His:SAE2UFDCt and perform subsequent MS analysis ...
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bioRxiv - Neuroscience 2021Quote: ... washed 3 x 10 min in PBS-TX (0.2% Triton X-100 in 0.1M PBS) and incubated with the primary antibody rabbit anti-CTB overnight (1:3000) (Sigma-Aldrich, UK). Sections were washed for 3 x 10 min (PBS-TX ...
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bioRxiv - Neuroscience 2021Quote: ... the sections were incubated for 48 hours at 4°C with the following primary antibodies: anti-GBA (1:500; human 8E4; human and rodent G4171, Sigma-Aldrich), anti-human α-synuclein (syn211 ...
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bioRxiv - Neuroscience 2021Quote: ... Sections were then incubated in primary antibody diluted in blocking solution (PBST, 1% BSA) overnight at 4°C with the following primary antibodies: mouse anti-NeuN (1:500, Millipore, Mab377), rat anti-BCL11B (1:1,000 ...
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bioRxiv - Neuroscience 2021Quote: ... sections were incubated overnight at RT with a combination of two primary antibodies: a mouse monoclonal anti-PCNA (1:500; Sigma-Aldrich; catalogue number P8825 ...
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bioRxiv - Molecular Biology 2021Quote: ... The membranes were blocked with 5% non-fat milk and incubated with the following antibodies: anti-FLAG (1:1000, Cat. #F1804; Sigma-Aldrich), and anti-HA (1:1000 ...
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bioRxiv - Microbiology 2021Quote: ... Membrane was then washed 3x for 10 min with TBST and subsequently incubated with the alginate antibody (Sigma, monoclonal, anti-mouse) overnight at 4°C ...