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Citations for Millipore Sigma :
7101 - 7150 of 10000+ citations for Anti IL 4I1 Antibody since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2022Quote: ... The membrane was blocked with 5% milk in TBS-T (TBS with 1% Tween-20) and incubated over night at 4°C with the primary anti-FLAG antibody (Sigma F1804-200UG ...
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bioRxiv - Cancer Biology 2022Quote: ... The primary antibodies used were as follows: mouse anti-phospho-Histone H2A.X (Ser139; clone JBW301; Sigma, 05-636; 1/1,000), mouse tubulin (clone B-5-1-2 ...
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bioRxiv - Cancer Biology 2022Quote: ... diluted 1:1000 in casein and incubated overnight at 4 degrees Celsius with shaking OR Monoclonal Anti-Beta-Actin mouse antibody (Sigma) diluted 1:5000 in casein and incubated at room temperature for 1 hour ...
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bioRxiv - Cancer Biology 2022Quote: ... Samples were afterwards incubated with primary antibodies overnight hours at 4 °C at the following dilutions: anti-tubulin β3 (1:1000; Sigma), anti-GFAP (1:1000 ...
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bioRxiv - Biophysics 2022Quote: ... the acini were incubated with the primary antibody mouse anti-podocalyxin (EMD Millipore, clone 3F2:D8, working dilution 1:200), mouse β1 integrin (Invitrogen ...
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bioRxiv - Biochemistry 2022Quote: ... The elution was separated by 8% Nu-PAGE and analyzed by CBB staining or immunoblotting using an anti-Flag antibody (Sigma).
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bioRxiv - Biophysics 2022Quote: ... The pre-cleared lysates were then incubated with 100 µL of antibody/protein A complexed beads (anti-FLAG [mouse, Sigma, F1804] or anti-HA [mouse ...
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bioRxiv - Cell Biology 2022Quote: The following monoclonal antibodies were used for immunofluorescence: anti-acetylated tubulin (mouse; clone 6-11B-1; Sigma-Aldrich; 1:500), anti-ubiquitin clone FK2 (mouse ...
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bioRxiv - Genetics 2022Quote: ... Binding of the anti-FLAG M2 primary antibody (mouse, Sigma-Aldrich, F3165, diluted 1:3000 in PBS-T+1%BSA) was allowed in a humidity chamber (overnight ...
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bioRxiv - Microbiology 2022Quote: ... The membrane was washed three times with PBS + 0.05% Tween-20 and then incubated with PBS + 1% milk solution containing a 1:10.000 anti-rabbit horseradish peroxidase conjugated secondary antibody (A0545, Sigma-Aldrich). The membrane was washed three times with PBS + 0.05% Tween-20 and then incubated for 5 min with Pierce ECL Plus substrate (Thermo Scientific) ...
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bioRxiv - Neuroscience 2022Quote: ... Detection was carried out using anti-mouse IgG-HRP antibody (GE; 1:5000 in PBS + 2% BSA) followed by addition of TMB substrate (Sigma). Reaction was stopped by adding 0.5 M H2SO4 ...
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bioRxiv - Neuroscience 2022Quote: ... The following primary antibodies were used: (1) rabbit anti-gamma amino butyric acid (GABA; Sigma-Aldrich #A2052, 1:1000 dilution) (2 ...
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bioRxiv - Microbiology 2022Quote: ... The wells were then washed with ice-cold PBST and incubated with HRP-linked goat anti-chicken antibody (Sigma-Aldrich) for 2 h at 4°C ...
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bioRxiv - Microbiology 2022Quote: ... SY0917 (MA11010) SABC-Serum) (rabbit, 1:10,000) and a secondary anti-rabbit-HRP (goat) conjugate antibody (1:10,000, Sigma-Aldrich, #31460). The rest of the samples was prepared for mass spectrometric analysis as previously described (Hör et al. ...
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bioRxiv - Plant Biology 2022Quote: ... Membranes were then incubated overnight at 4 °C with 10 ml TBS-T containing either anti-c-myc-peroxidase antibody (1:10,000; Sigma, A5598), or anti-HA primary antibody (1:10,000 ...
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bioRxiv - Plant Biology 2022Quote: ... followed by incubation in a 1:10000 dilution of the fluorescein Dylight 405 labelled goat anti-rabbit secondary antibody (Sigma) in PBS-BSA for 2 h at 25°C ...
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bioRxiv - Plant Biology 2022Quote: ... SDS-PAGE/Western blot analysis was used to identify proteins in the sample with use of anti-FLAG M2 antibody (Sigma) and anti-MYC antibody (Santa Cruz Biotechnology ...
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bioRxiv - Neuroscience 2022Quote: ... Binding of the FLAG-tagged DARPins to streptavidin-immobilized biotinylated gephyrin variants was measured using a mouse-anti-FLAG-M2 antibody (Sigma) as 1st and goat-anti-mouse-alkaline phosphatase conjugated antibody (Sigma ...
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bioRxiv - Neuroscience 2022Quote: ... HA-tag fluorescence HA-tagged SaCas9 was visualized using the following antibodies: rabbit anti-HA-tag (Sigma-Aldrich, #H6908, RRID:AB_260070) and secondary antibody anti-rabbit Alexa Fluor 488 (1:1000 ThermoFisher ...
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bioRxiv - Microbiology 2022Quote: ... probed with a 1:10,000 dilution of an anti-guinea pig-horseradish peroxidase-conjugated secondary antibody (Sigma; product no. A5545) for 30 min at ambient temperature ...
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bioRxiv - Microbiology 2022Quote: ... RBD or spike-bound serum antibodies were detected with horseradish peroxidase conjugated goat anti-mouse IgG (1:500 dilution, Milipore Sigma) incubating for 2 h at 25°C ...
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bioRxiv - Neuroscience 2023Quote: ... Afterwards the samples were incubated with primary antibodies (anti-β-tubulin III produced in rabbit (Sigma, Ireland, T2200, 1:400) and anti-GFAP produced in mouse (Sigma ...
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bioRxiv - Neuroscience 2022Quote: ... membranes were re-probed with the HRP-conjugated primary anti-β-actin antibody for 20 min (Millipore-Sigma, 1:50,000), and the β-actin immunoreactivity was visualized.
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bioRxiv - Molecular Biology 2023Quote: ... HRP-conjugated anti-rabbit (catalog no. 401315) and anti-mouse (catalog no. 401215) secondary antibodies and BSA (catalog no. 12659) were from Millipore. All antibodies were diluted in 1% BSA dissolved in PBS-T (1.5 mM Sodium Phosphate Monobasic ...
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bioRxiv - Immunology 2022Quote: ... primary antibodies were incubated overnight at 4°C: mouse monoclonal anti-C5aR1 (clone: S5/1; Millipore; cat. MABF1980; 1:50), rabbit polyclonal anti-IBA1 (FUJIFILM Wako Pure Chemical Corporation ...
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bioRxiv - Neuroscience 2023Quote: ... for 30 minutes and then incubated for 48 hours at room temperature in goat anti-ChAT primary antibody (1:1000, AB144P, Millipore). Following rinse ...
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bioRxiv - Neuroscience 2022Quote: ... To assess the NOTCH3 ECD deposits the tissues were incubated overnight at 4 °C with mouse monoclonal anti-human NOTCH3 ECD primary antibody (1:100, clone 1E4, Millipore) followed by detection with Alexa 594 conjugated anti-mouse secondary antibody (1:1000 ...
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bioRxiv - Neuroscience 2022Quote: ... Washing steps were performed using PBS-Tween buffer followed by horseradish peroxidase-conjugated goat anti-mouse IgG antibody (Sigma-Aldrich) for 30 minutes at room temperature ...
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bioRxiv - Molecular Biology 2022Quote: ... followed by probing with respective goat anti-mouse or rabbit secondary antibodies conjugated with alkaline phosphatase (dilution 1: 5,000; Sigma-Aldrich) or conjugated with HRP (dilution 1:2000 ...
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bioRxiv - Neuroscience 2022Quote: ... α-tubulin antibody was used as loading control (mouse anti-α-tubulin, 1:10000; Sigma-Aldrich cat#T6074, RRID: AB_477582). Antibodies were functionally validated by Cell Signalling Technology and Sigma-Aldrich companies ...
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bioRxiv - Molecular Biology 2022Quote: ... HRPA-1::AID*::TEV::FLAG was detected using Monoclonal Anti-FLAG M2-Peroxidase (HRP) antibody (A8592-2MG) from Sigma-Aldrich at a 1:1000 dilution ...
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bioRxiv - Molecular Biology 2022Quote: ... followed by donkey anti-rabbit secondary antibody conjugated to horseradish peroxidase (“HRP”, Sigma GENA934, 1:5,000 in 1% milk-PBST). Aldolase was detected with anti-aldolase-HRP (Abcam ab38905 ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... membranes were incubated with horseradish peroxidase-conjugated secondary antibodies in the blocking buffer for 1 h at room temperature (1:3,000 rabbit anti-goat, Sigma-Aldrich #A5420 or 1:3,000 goat anti-mouse ...
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bioRxiv - Plant Biology 2022Quote: ... The proteins were separated by 10 % SDS-PAGE gels and detected by immunoblotting with the first anti-GFP or HA-tag antibodies (Sigma) and the second anti-rabbit IgG-peroxidase antibody (Sigma) ...
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bioRxiv - Molecular Biology 2022Quote: ... 0.1% triton-X 100 in PBS at room temperature for one hour and incubated overnight in 5% FBS and 0.1% triton-X 100 in PBS at 4°C with the following antibodies: anti-LIMCH1 (1:200, catalog number HPA063840, Sigma), anti-mLIMCH1 (1:200 ...
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bioRxiv - Molecular Biology 2022Quote: ... for one hour and incubated overnight at 4°C in 5% milk/PBS-T with the following antibodies: anti-LIMCH1 (1:400, catalog number HPA063840, Sigma), anti-MBNL1 (1:1,000 ...
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bioRxiv - Molecular Biology 2022Quote: ... Golgi apparatus was detected with mouse anti-Golgi 58K protein /formiminotransferase cyclodeaminase (FTCD) antibody (G2404, SIGMA-Aldrich, Prague, Czech Republic). The following secondary antibodies were used for detection ...
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bioRxiv - Cell Biology 2022Quote: ... Primary antibodies for all stainings were as follows: rabbit anti-TGFA (HPA042297, Sigma-Aldrich, dilution 1:50 and 1:100), rabbit polyclonal anti-pEGFR clone Tyr845 (07-820 ...
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bioRxiv - Molecular Biology 2024Quote: The following monoclonal and polyclonal antibodies were used: anti-β-actin (WB: 1 µg/ml, AC-15/A1978, Sigma-Aldrich), anti-Miro1/2 (WB ...
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The conserved membrane-proximal domain of Sbh1/ Sec61β guides signal peptides into the Sec61 channelbioRxiv - Biochemistry 2024Quote: ... both the excised control spots and the peptide membranes were incubated with HRP-tagged anti-Biotin antibody (1:10,000, Sigma-Aldrich) and washed thrice again with Binding Buffer ...
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bioRxiv - Biochemistry 2024Quote: ... The cells were incubated with mouse anti-FLAG primary antibody (1:1000 dilution in PBS + 0.1% BSA; Sigma– Aldrich, F3165) at room temperature for 30 min and washed three times with PBS + 0.1% BSA ...
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bioRxiv - Cancer Biology 2024Quote: ... Protein extracts of puromycin pulsed and non-puromycin pulsed control cells were subject to immunoblot (as above) using anti-puromycin antibody (clone 12D10, 1:1000, Sigma).
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bioRxiv - Cell Biology 2024Quote: ... cultures were incubated for 1 h at room temperature with primary antibodies as follows: mouse anti-laminA/C (Sigma SAB4200236), mouse anti-phospho-histone H2AX (Merck 05-636) ...
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bioRxiv - Molecular Biology 2024Quote: Primary antibodies used in this study were: mouse anti-FLAG M2 (Sigma-Aldrich #F3165, RRID: AB_259529, 1:1000-1:2500), mouse anti-α-tubulin (Cell Signaling Technologies #3873 ...
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bioRxiv - Molecular Biology 2024Quote: ... 0.1% Tween-20 (TBS-T) and incubated for one hour at RT with Mouse anti-FLAG (M2) antibody-HRP conjugated (Sigma) diluted 1:4000 in blocking milk ...
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bioRxiv - Molecular Biology 2024Quote: ... the BG4 antibody67 was incubated for 1 hour at 37°C and subsequently incubated with 1:800 mouse anti-FLAG antibody (F1804, Sigma) to BG4 and 1:500 rabbit anti-PARP1 (ab227244 ...
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bioRxiv - Immunology 2024Quote: ... the plates were washed three times and a solution of anti-nucleoprotein antibodies (Sigma, #MAB8257 and #MAB8258, 1:4000 each) in antibody diluent were added for 1 hour at room temperature ...
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bioRxiv - Neuroscience 2024Quote: ... Tissues were then transferred to a ChAT primary and TrkA primary antibody solution with blocking buffer (1:200 dilution goat anti-ChAT polyclonal AB143, Millipore EMD ...
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bioRxiv - Neuroscience 2024Quote: ... followed the same staining and mounting protocol described above but were incubated ON with a dilution of 1/2,000 anti-HA rabbit antibody (Sigma; H6908), and a 1/1000 dilution of AlexaFluor-488 donkey anti-chicken (Jackson InmunoResearch ...
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bioRxiv - Neuroscience 2024Quote: ... Sections were incubated overnight at 4 °C with antibodies that recognise specific proteins expressed by each cell type such as mouse anti-NeuN (1:250; Millipore) to label neurons ...