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Citations for Millipore Sigma :
7301 - 7350 of 10000+ citations for Anti IL 4I1 Antibody since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2019Quote: ... 1 μL of antibody (α-Flag-M2 antibody, Sigma-Aldrich / α-HA ...
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bioRxiv - Molecular Biology 2021Quote: ... V5 antibody (Thermo Pierce) and FLAG antibody (M2, Sigma-Aldrich) were diluted 1:5000 for use as the primary antibody ...
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bioRxiv - Developmental Biology 2020Quote: ... primary antibodies and HRP-conjugated secondary antibodies (Cat# A9044, Sigma).
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bioRxiv - Microbiology 2020Quote: ... cells were detached by 1mM EDTA in PBS and then stained by 4 ug/ml of anti-Flag M2 antibody (Sigma, F1804) and 2 ug/ml of Goat anti-mouse IgG (H+L ...
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bioRxiv - Biophysics 2021Quote: U2OS cell immunostained for microtubules for DNA-PAINT imaging were prepared as previously described6 using anti beta-tubulin antibody (T5293; Sigma-Aldrich).
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bioRxiv - Biophysics 2021Quote: ... the cells were collected and blocked with 5% BSA in PBS at RT for 15 min and incubated with primary anti-Flag antibody (1:300, Sigma-Aldrich) at RT for 1 h ...
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bioRxiv - Cancer Biology 2021Quote: ... and nascent puromycylated proteins was detected by Western blot analysis as above using a mouse anti-puromycin antibody 12D10 (1:3000)(MABE343, Sigma-Aldrich).
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bioRxiv - Neuroscience 2021Quote: ... filtered through a cell strainer and stained with a 1:1000 dilution of the anti-NeuN antibody (clone A60, Alexa Fluor 488 conjugated, Millipore MAB377X) for 45 minutes by rotation in the dark at 4°C after adding BSA to a final concentration of 0.1% ...
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bioRxiv - Cancer Biology 2021Quote: ... All ChIPs were performed using 500 μg of extract and 2 μg of antibody per sample (anti-KMT2D/MLL4, Sigma # HPA035977). Thirty microliters of Protein G Dynabeads was used per ChIP ...
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bioRxiv - Cell Biology 2020Quote: ... Cells were then incubated overnight at 4°C in a wet chamber with the following antibodies: anti-P2X7 (cat. n. P8232, Sigma-Aldrich) and anti-Tom 20 (cat n ...
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bioRxiv - Cell Biology 2019Quote: ... Protein translation was analyzed on the WES automated western blotting system (ProteinSimple) using anti-Puromycin antibody (1:50; Sigma-Aldrich, MABE343).
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bioRxiv - Cell Biology 2020Quote: ... The proteins in both soluble fraction and membrane fraction were separated in 12% SDS-PAGE gel and analysed by western blotting using anti-GFP polyclonal antibody (1:4000; Sigma-Aldrich).
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bioRxiv - Cell Biology 2020Quote: ... was then added to cells for 20 minutes and subsequently replaced with an anti-FLAG antibody (1:1000 in blocking solution) (Cat #F3165, Sigma-Aldrich) for one hour ...
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bioRxiv - Cell Biology 2019Quote: ... Samples were blocked for at least 2.5 hours at room temperature followed by the addition of poly-clonal rabbit anti-histone H4 (citrulline 3) antibody (EMD Millipore) used at 1:100 and incubated overnight at 4°C ...
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bioRxiv - Cell Biology 2019Quote: ... A polyclonal rabbit anti-AGPAT (SAB2700797) and a monoacylglycerol lipase An MAGL rabbit polyclonal antibody (Cat. 011994) were purchased from Sigma-Aldrich. A rabbit anti-ATGL (mAb ab207799 ...
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bioRxiv - Cell Biology 2020Quote: ... The membrane was washed four times (each for 10 min) in TBS-T before being incubated with an anti-mouse horse radish peroxidase (HRP)-conjugated secondary antibody (A9044, Sigma-Aldrich) or an anti-rabbit HRP-conjugated secondary antibody (31466 ...
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bioRxiv - Cell Biology 2020Quote: Lamin A proteins were detected by probing with mouse anti-Myc antibodies (clone 4A6, Millipore cat #05-724; 1:10,000 dilution) decorated with goat anti-mouse secondary IRDye 680RD antibodies (LI-COR ...
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bioRxiv - Developmental Biology 2021Quote: ... samples were incubated overnight at 4°C on a rocking platform with the primary antibody anti-H3K9ac (1:1,000; Sigma, cat. # H9286) or anti-H3K9bhb (1:1,000 ...
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bioRxiv - Developmental Biology 2021Quote: Innervation analysis was done on regenerating limb and flank sections to quantify innervation abundance using the Mouse Monoclonal Anti-Acetylated Tubulin antibody (1:200 dilution – Sigma Aldrich), followed by the Goat-Anti-Mouse IgG Alexa Fluor 488 (1:200 dilution – Abcam ...
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bioRxiv - Developmental Biology 2020Quote: ... the blot was washed in PBST and incubated in primary antibodies of rabbit polyclonal anti-TBX5 at a 1:400 dilution (Sigma #HPA008786) and mouse monoclonal anti-cTNT at 1:1000 dilution (ThermoFisher Scientific #MS-295-P) ...
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bioRxiv - Molecular Biology 2021Quote: ... 10 % lysate was saved as input and IP was performed using an anti-pan-AGO antibody (clone 2A8, MABE56 Sigma-Aldrich) and an anti-FLAG M2 antibody (F3165 ...
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bioRxiv - Developmental Biology 2021Quote: ... followed by immunocytochemistry with mouse monoclonal anti-vinculin antibody (1:200 dilution in 1% BSA–PBS) (Sigma; V9264, clone hVIN-1) and Alexa 555–labelled goat anti–mouse IgG (#A21422 ...
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bioRxiv - Developmental Biology 2021Quote: Analysis of nerve development in E10.5 embryos was performed using a monoclonal anti-neurofilament 160 (NF160) antibody (#N5264, Sigma Aldrich, USA) as previously described (Clouthier et al. ...
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bioRxiv - Microbiology 2020Quote: ... The cells were then incubated for 1hr with primary antibodies diluted as follows with Block buffer: mouse monoclonal anti-FLAG (Sigma Aldrich) at 1:500 ...
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bioRxiv - Molecular Biology 2022Quote: ... GPR126 and empty vector-transfected cells were stimulated with the indicated concentrations of anti-HA antibody (H3663, stock concentration 1 mg/ml, Sigma-Aldrich), anti-HA Fab fragment (stock concentration 1 mg/ml ...
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bioRxiv - Molecular Biology 2022Quote: Tipless silicon nitride AFM cantilevers (NanoWorld, PNP-TR-TL) were coated with monoclonal anti-HA antibodies produced in mouse (H3663, Sigma-Aldrich) or Fc-control for the antibody-based mechano-activation experiments using flexible PEG spacers as described before (Ebner et al. ...
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bioRxiv - Molecular Biology 2022Quote: ... followed by incubation with 3-isobutylmethyl-xanthine (1 mM)-containing medium or including the secondary antibody anti-mouse IgG (Fc specific, M2650, Sigma-Aldrich) for 30 min ...
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bioRxiv - Neuroscience 2022Quote: ... retinas were incubated again in blocking solution with primary antibody against choline acetyltransferase (ChAT; Millipore, AB144P, goat anti-ChAT, 1:1000) for 5 nights at 4°C ...
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bioRxiv - Neuroscience 2021Quote: ... We use a combination of antibodies (rabbit anti-parvalbumin, 1:5000, PV235, Swant + biotin-conjugated lectin from WFA, 1:250, L-1516, Sigma-Aldrich). On the day after ...
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bioRxiv - Neuroscience 2021Quote: ... for 20min and rescanned to ensure loss of signal before being incubated with an anti-GAFPH antibody (Sigma, 45-G9545-100UL) as a loading control ...
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bioRxiv - Neuroscience 2021Quote: ... washed as above described and re-blotted using mouse anti-β-actin antibody (Sigma-Aldrich, USA; 1:2500, 1 h, RT). Again ...
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bioRxiv - Evolutionary Biology 2022Quote: ... three technical replicates for each sample were analyzed by SDS-PAGE and Western blot using Anti-FLAG® antibody (Sigma-Aldrich Monoclonal ANTIFLAG® M2-Peroxidase (HRP ...
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bioRxiv - Genomics 2019Quote: ... GFP+ cells were FACS sorted and expression of the RRM-FLAG construct was confirmed by Western Blot using an anti-FLAG antibody (Sigma F3165). SpenRRM-FLAG expressing cells were treated with 10 mM HDAC1/2 inhibitor (Cayman Chemical Company ...
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bioRxiv - Neuroscience 2019Quote: ... blocked using 1% BSA in TBST and incubated with primary anti-TH antibody (dilution 1:500; AB152, Merck Millipore, Schaffhausen, Switzerland). 24h later ...
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bioRxiv - Physiology 2020Quote: ... Proteins were detected after incubation of the membranes with secondary GP anti-rabbit alkaline phosphatase-conjugated antibody (1:30000, cat# A3687, Sigma Aldrich), GP anti-mouse alkaline phosphatase-conjugated antibody (1:20000 ...
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bioRxiv - Cell Biology 2019Quote: ... anti-GFP (in house made rabbit polyclonal antibody; 1:1,000-4,000) anti-Vinculin (mouse monoclonal; clone hVIN-1; Sigma-Aldrich; 1:10000), anti PMF1/NNF1 (in house made mouse affinity purified monoclonal ...
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bioRxiv - Immunology 2019Quote: ... cells were washed twice with PBS for 1 h at 37°C and then incubated with a FITC-conjugated rabbit anti-mouse IgG antibody (Sigma-Aldrich) at a 1:500 dilution ...
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bioRxiv - Molecular Biology 2019Quote: 90 µg of MRC5 chromatin extracts were incubated overnight at 4° C in 150 μl total volume of IP buffer (see above) with antibody (RNAP1: anti-RPA194, sc-48385, Santa Cruz; NMI: M3567 Sigma) (ChIP ...
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bioRxiv - Molecular Biology 2019Quote: ... solution and incubated for 1.5 h RT or overnight at 4° C with the primary antibodies in milk PBS-T (RNAP1: anti-RPA194, sc-48385, Santa Cruz; ACTβ: A5316, Sigma or ab8227, Abcam; NMI: M3567, Sigma; α-Tubulin ...
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bioRxiv - Molecular Biology 2019Quote: ... solution and incubated for 1.5 h RT or overnight at 4° C with the primary antibodies in milk PBS-T (RNAP1: anti-RPA194, sc-48385, Santa Cruz; ACTβ: A5316, Sigma or ab8227 ...
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bioRxiv - Genetics 2019Quote: ... cells on coverslips were fixed in 4% PFA for 20 min and blocked with 3% BSA in PBS for 10 min before applying Ret-P1 anti-rhodopsin antibody (Sigma #O4886) at a final concentration of 1:1000 in blocking buffer for 1 hr ...
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bioRxiv - Immunology 2020Quote: ... nitrocellulose membranes were probed with protein-specific primary antibodies diluted in PBS containing 5% Milk or 5% BSA as follows: anti-actin Cat #A5441 from Sigma Aldrich; anti-tubulin Cat #5286 from Santa Cruz ...
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bioRxiv - Cancer Biology 2019Quote: ... the TMA slide was subjected to Cell Conditioning 1 (CC1, Ventana) antigen retrieval and stained with a prediluted anti-MCT antibody (Mouse, Clone G3, Millipore #MAB1222) using an automated immunostainer ...
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bioRxiv - Genetics 2019Quote: Cell extracts were prepared by TCA precipitation and proteins were separated by 10% SDS-PAGE followed by immunoblotting with anti-HA antibody (Sigma-Aldrich, ref ...
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bioRxiv - Molecular Biology 2019Quote: ... NF-kB (CST, #3033), PCM1 (CST, #5213) and GABARAP (CST, #26632) and NDP52 (. Secondary antibodies: anti-Mouse IgG–Peroxidase (Sigma, A5906), anti-Rabbit IgG–Peroxidase (Sigma ...
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bioRxiv - Physiology 2020Quote: ... containing 5% w/v non-fat dry milk followed by anti-rabbit IgG HRP-conjugated goat secondary antibody (A0545, Sigma-Aldrich). Signal was detected using the SuperSignal West Dura Extended Duration Substrate (Thermo Fisher Scientific ...
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bioRxiv - Molecular Biology 2020Quote: ... 1% of pre-cleared chromatin was set aside for input and 5 µg Monoclonal ANTI-FLAG® M2 antibody (to capture FLAG-tagged dCas9) or IgG (Sigma) was added to the remaining volume and then incubated at 4°C on a nutator overnight ...
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bioRxiv - Cancer Biology 2019Quote: ... Immunoprecipitates were then washed three times with cold Triton lysis buffer and were analyzed by western blot using an anti-His antibody (Sigma Aldrich).
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bioRxiv - Microbiology 2019Quote: ... The plate was washed three times with wash buffer (PBS/0.1% Tween-20) and the primary antibody (anti-NP mouse monoclonal Ab, Millipore, cat. MAB2851) added in blocking buffer ...
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bioRxiv - Biophysics 2019Quote: ... the cells were incubated for 1 hour at 37°C with 1:300 rabbit anti-β-tubulin antibody (Sigma Aldrich, T5293) in PBS + 1% BSA ...