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Citations for Millipore Sigma :
7001 - 7050 of 10000+ citations for Anti IL 4I1 Antibody since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Synthetic Biology 2020Quote: ... and incubated for 1 h at room temperature or overnight at 4°C in primary antibody (Mouse-anti-FLAG M2 [Sigma #F1804 ...
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bioRxiv - Animal Behavior and Cognition 2020Quote: ... and incubated on an orbital shaker for 24h at room temperature + 72h at 4 °C in primary antibody (polyclonal rabbit anti-ERα, Merck-Millipore, cat ...
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bioRxiv - Molecular Biology 2020Quote: ... Antibodies and dilutions for western-blot were the following: rabbit polyclonal anti-RPAP3 at 1:2000 dilution (Sigma-Aldrich, SAB1411438); monoclonal mouse anti-TRBP at 1:500 dilution (Abcam ...
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bioRxiv - Genetics 2020Quote: ... Cells were incubated overnight at room temperature in blocking buffer containing primary antibody (mouse anti-UBE3A (Sigma SAB1404508 clone 3E5)) at 1:250) ...
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bioRxiv - Pathology 2020Quote: ... IP was performed by incubation of the resulting lysates overnight at 4 °C with mouse monoclonal anti-Flag antibody (Cat.# F3165, Sigma) or with normal mouse IgG (Cat.#A7028 ...
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bioRxiv - Molecular Biology 2020Quote: ... 500 μg of the whole cell extract was rotated overnight at + 4°C with 2.5 μg of the anti-FLAG antibody (Sigma F1804) and the protein-immunocomplexes were separated with the Dynabeads Protein G magnetic beads (ThermoFisher Scientific) ...
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bioRxiv - Genetics 2020Quote: ... and were then used for pull-downs with the respective antibody-conjugated beads: either the anti-FLAG M2-FLAG affinity gel (Sigma) (20 μl per IP ...
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bioRxiv - Molecular Biology 2020Quote: 100 μl nuclear extract diluted in lysis buffer to a final volume of 200 μl was incubated with 2μg of anti-CCDC84 antibody (SIGMA-HPA071715) overnight in the cold room with end-to-end rotation ...
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bioRxiv - Neuroscience 2021Quote: ... Slices were incubated free-floating overnight at RT with the mouse G10 anti-reelin primary antibody (1:3000, MAB5406; Millipore) diluted in the blocking solution ...
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bioRxiv - Neuroscience 2021Quote: Immunohistochemical staining was performed on free-floating brain sections using the following primary antibodies: mouse anti-rat TH (1:4000, MAB318, Merck Millipore), rabbit anti-Girk2 (1:500 ...
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bioRxiv - Plant Biology 2020Quote: ... The sheared chromatin was immunoprecipitated overnight using the following antibodies and dilutions: anti-LexA BD (Millipore 06-719, 1:300) Anti-H3K27me3 (Millipore ...
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bioRxiv - Neuroscience 2020Quote: ... 3% Triton-X in PBS) for 1 h before incubation with primary antibodies (1:1000 anti-tyrosine hydroxylase, #AB152, Millipore UK ...
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bioRxiv - Genetics 2020Quote: ... 150mM NaCl) including 3% milk powder for 1 hour and incubated overnight with anti-FLAG antibody (Sigma Aldrich, cat. # F1804) in TBS plus 3% milk ...
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bioRxiv - Microbiology 2019Quote: ... This was followed by four washes with 200 µl of incubation buffer and incubation with 50 µl of anti-His-HRP antibody (Sigma), diluted 1:2000 in incubation buffer for 1 hr at 24°C ...
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bioRxiv - Immunology 2021Quote: ... blocked in 5% BSA for 30 min and incubated ON with mouse anti-GAD67 monoclonal antibody (1:6000, MAB5406, Merck Millipore) or 1 h with rabbit polyclonal anti-GAPDH antibody (1:3000 ...
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bioRxiv - Biochemistry 2021Quote: ... Samples from the key steps of the purification were subjected to western blot analysis (anti-3x-FLAG antibody A8592, Sigma) (Supplementary Fig ...
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bioRxiv - Cancer Biology 2021Quote: ... and 0.05% (v/v) Tween-20 in PBS) and incubated with primary antibody anti-LIG3 (1:150, Sigma-Aldrich, #HPA006723) in staining buffer for 2hr at RT ...
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bioRxiv - Molecular Biology 2021Quote: ... cells expressing GIPR and GLP-1R were incubated with 1:300 anti-Flag primary antibody (diluted with PBS containing 5% BSA, Sigma), and those expressing GCGR were incubated with 1:50 anti-GCGR antibody (diluted with PBS containing 5% BSA ...
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bioRxiv - Microbiology 2019Quote: ... A template encoding an M2 tagged peptide was added to the reaction which was then resolved by SDS-PAGE and probed with an anti-M2 antibody (Sigma). Further details are described in supplemental material.
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bioRxiv - Microbiology 2020Quote: ... and the lysate/antibody/bead mix was incubated at 4°C overnight with continuous rotation Beads were then washed in HEPES buffer and boiled in Laemmli loading buffer prior to SDS PAGE and western blot analysis with an anti-HA antibody (HA-7; H9658, Sigma).
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bioRxiv - Microbiology 2020Quote: ... The grids were washed once with 1% BSA in DPBS and incubated with a 1:10 dilution of rabbit anti-c-Myc antibody (Sigma) for 1 h ...
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bioRxiv - Microbiology 2021Quote: ... FLAG-tagged protein detection was performed as previously reported [71] using an Anti-FLAG M2-Peroxidase (HRP) antibody (Sigma, A8592) and bands were visualized using an ImageQuant LAS 4000 imager.
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bioRxiv - Microbiology 2021Quote: ... Sera were incubated for one hour at room temperature followed by three washes with PBST before incubating with an anti-mouse IgG secondary antibody conjugated to alkaline phosphatase (Sigma) used as a 1:5000 dilution for one hour ...
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bioRxiv - Microbiology 2021Quote: ... Non-infected and Lm-infected HUVEC were incubated for 1 h with primary antibody rabbit anti-STAB-1 (1:100, Millipore), diluted in 0.2% saponin (Merck ...
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bioRxiv - Microbiology 2021Quote: ... ScAb or mAb samples were incubated with the antigen in doubling dilutions and the binding was detected using anti-Human C Kappa horseradish peroxidase (HRP) conjugated secondary antibody (Sigma) (for scAbs ...
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bioRxiv - Synthetic Biology 2019Quote: ... Blocking solution was discarded and replaced with 30 mL of 3% BSA TBST buffer then 7.8 µL of 3.85 mg/mL anti-FLAG antibody (Sigma-Aldrich, F3165) was added ...
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bioRxiv - Synthetic Biology 2020Quote: ... Expression of GAPDH was used as a loading control using a mouse anti-GAPDH antibody (1:10000 dilution, MAB374, Sigma) and IRDye 680CW goat anti-mouse IgG secondary antibody (1:5000 dilution ...
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bioRxiv - Genetics 2020Quote: ... 3xFLAG-EED expression and deletion of eed were confirmed by western blots with anti-FLAG antibody (Sigma Aldrich, cat. # F1804) and genotyping with eed deletion primers (see above) ...
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bioRxiv - Pathology 2021Quote: ... postmortem kidney was fixed in 20% formalin for 2 weeks and processed for free-?oating preparation as done for mouse tissue with one exception that anti-human CD31 antibody (CBL468, 1:20 dilution, Millipore) was used.
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bioRxiv - Systems Biology 2020Quote: ... Immunoprecipitation was carried out by incubating the lysate with the monoclonal anti-FLAG M2-mouse primary antibody (Sigma Aldrich, F1804) and protein G Dynabead (Invitrogen ...
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bioRxiv - Cell Biology 2020Quote: ... and the mouse monoclonal and rabbit polyclonal anti-phosphorylated histone H3 (Ser10) antibodies (ref. 06570, 1:1000) were obtained from Millipore. The anti-GFP mouse monoclonal antibody mix (ref ...
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bioRxiv - Synthetic Biology 2020Quote: ... The membrane was then washed several times with Tris-Buffered Saline with 0.1% Tween 20 (TBST) before a 30 in incubation with monoclonal Mouse Anti-FLAG M2-Peroxidase (HRP) antibodies (Sigma-Aldrich) diluted 1:5000 in TBST ...
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bioRxiv - Biochemistry 2020Quote: ... Tie2‐FLAG from IP was then subjected to immunoblotting analysis of phospho‐Tie2 measured by anti‐phosphotyrosine antibody blotting (4G10 Platinum, Millipore).
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bioRxiv - Neuroscience 2020Quote: ... Sections were then incubated overnight at 4°C in blocking solution containing one of the following primary antibodies: anti-GFAP (1:2000; Merck Millipore) and anti-Iba1 (1:500 ...
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bioRxiv - Plant Biology 2020Quote: ... were isolated from 2-week-old dPPRrbcL-1 or Col-0 plants and incubated with monoclonal anti-HA antibodies (Sigma). IgGs were captured with Protein A DynaBeads (Thermo Fisher Scientific ...
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bioRxiv - Immunology 2021Quote: ... plates were again washed thrice followed by incubation with 1:5,000 dilution of horse radish peroxidase (HRP) conjugated anti-guinea pig IgG secondary antibody (Sigma-Aldrich) or 1:2,000 dilution of HRP conjugated anti-mouse IgG secondary antibody (Sigma-Aldrich ...
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bioRxiv - Microbiology 2020Quote: ... Protein level of GTP-bound ARF6 and total ARF6 in cell lysates was detected by Western blot with an anti-ARF6 antibody (1:1000 in 5% milk) (Sigma).
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bioRxiv - Immunology 2019Quote: ... 0.1% [w/v] saponin in PBS) for 25 min at RT and then stained with mouse anti-flavivirus antibody (clone D1-4G2-4-15, Millipore) diluted 1:500 in FACS wash buffer for 90 min at RT ...
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bioRxiv - Plant Biology 2019Quote: ... the blot was washed with 1×TBS-T solution (4×5min) and incubated with anti-Mouse IgG-HRP secondary antibody solution (Sigma, 1:10,000 dilution in 1×TBS-T with 0.2% Blocking Reagent [Roche] ...
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bioRxiv - Developmental Biology 2020Quote: HRP-conjugated secondary antibodies for Western blotting: goat anti-mouse IgG (Cat# A 4416, Sigma-Aldrich, St. Louis, MO, USA) and goat anti-rabbit IgG (Cat# GtxRb-003-EHRPX ...
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bioRxiv - Biochemistry 2019Quote: ... Proteins were resolved in SDS-PAGE gels and transferred to nitrocellulose membranes that were incubated overnight at 4 °C with one of the following antibodies: mouse monoclonal anti-HA (1:2000, Sigma); rabbit polyclonal MBP-probe (1:1000 ...
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bioRxiv - Cell Biology 2020Quote: ... Microtubules were immobilized in the flow cell by means of an anti-tubulin antibody (Sigma Aldrich, T7816, 10 µg/ml). Subsequently ...
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bioRxiv - Neuroscience 2019Quote: ... Nuclei were then labeled in a solution of anti-NeuN antibody conjugated to Alexa Fluor 488 (A60, Millipore, 1/1000) and 0.1% BSA ...
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bioRxiv - Microbiology 2019Quote: ... Plates were washed twice with PBS and incubated in same conditions with horseradish peroxidase (HRP)-conjugated goat anti-mouse secondary antibody (Sigma), diluted 1:1000 in blocking buffer ...
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bioRxiv - Biochemistry 2020Quote: ... The Flag-tagged receptor was detected using mouse anti-Flag M2 primary antibody (1:10,000 dilution; Sigma Aldrich, catalog #F3165) and IRDye® 680RD-conjugated donkey anti-mouse IgG secondary antibody (1:20,000 dilution ...
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bioRxiv - Biochemistry 2020Quote: ... HA-tagged [P2G]CXCL12 was detected using a rabbit anti-HA primary antibody (1:10,000 dilution; Sigma Aldrich, catalog #SAB4300603) and IRDye® 800CW-conjugated goat anti-rabbit IgG (1:20,000 dilution ...
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bioRxiv - Physiology 2020Quote: ... and probed overnight (4 °C) with one of the following primary antibodies: Anti-TSG-6 mouse monoclonal (1 μg/ml; Millipore); rat monoclonal TNFα (1 μg/ml ...
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bioRxiv - Pharmacology and Toxicology 2019Quote: ... TN) and the secondary antibody HRP-conjugated anti-goat IgG from rabbit was purchased from Sigma-Aldrich (St. Louis, MO).
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bioRxiv - Bioengineering 2019Quote: For Flag-tag detection membranes were incubated for two hours with the monoclonal anti-Flag M2 antibody from mouse (F3165, Sigma) in a 1:5000 dilution (in TBS-T ...
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bioRxiv - Cancer Biology 2020Quote: ... The equivalent of 5 mg of protein was further enriched for phosphorylated tyrosine peptides by overnight immunoprecipitation (pY-IP) using a cocktail of anti-phosphotyrosine antibodies (4G10, Millipore; PT-66 ...