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Citations for Millipore Sigma :
7051 - 7100 of 10000+ citations for Anti IL 4I1 Antibody since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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Significant control of Zika infection in macaques depends on the elapsing time after dengue exposurebioRxiv - Immunology 2019Quote: ... Plated were washed twice and incubated 1hr/37C/5%CO2/rocking with horseradish peroxidase (HRP)-conjugated goat anti-mouse antibody (Sigma), diluted 1:1,000 in blocking buffer ...
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bioRxiv - Microbiology 2020Quote: ... Cells were washed then sequentially incubated with anti-SARS-CoV-2 CR3022 antibody (Yuan et al., 2020) (1 μg/mL) and a HRP-conjugated goat anti-human IgG (Sigma) in PBS supplemented with 0.1% (w/v ...
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bioRxiv - Microbiology 2021Quote: ... The membrane was blocked (for 1 hr) and incubated for 1 hr with primary rabbit anti-FLAG antibody (Sigma-Aldrich). Following buffer wash the membrane was incubated for 1 hr with HRP-conjugated IgG mouse anti-rabbit antibodies (ThermoFisher Sci.) ...
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bioRxiv - Cell Biology 2020Quote: ... Cells were incubated with the following primary antibodies for 1 h at room temperature in Abdil: mouse anti-human lamin A/C (1:200; Millipore), rabbit anti-lamin A/C (1:200 ...
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bioRxiv - Cell Biology 2021Quote: ... 1 mg of extracted protein was transferred to Eppendorf tubes to incubate with anti-FLAG-antibody (1:1000; Millipore Sigma) or anti-HIS-antibody (1:300 ...
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bioRxiv - Physiology 2021Quote: ... Muscles were then incubated in blocking solution containing rabbit anti-heavy NF200 primary antibody (N4142 Sigma, Germany; 1:200 dilution) and anti-synaptophysin antibody (1:25 dilution as above) ...
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bioRxiv - Physiology 2020Quote: ... Immunofluorescence staining was performed using sheep anti-α-MSH antibody (diluted 1:1,000 in 0.1 M PB, ab5087, Millipore Sigma), and rabbit anti-AgRP antibody (diluted 1:1,000 in 0.1 M PB ...
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bioRxiv - Microbiology 2021Quote: Single-nucleotide EdU-labelled slides were incubated in blocking solution (1% BSA in PBS) for 30 mins and then 1:100 anti-centrin antibody (clone 20H5, Millipore) in blocking solution for 1 h at room temperature ...
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bioRxiv - Neuroscience 2021Quote: ... Immunoblotting was carried out with primary antibodies anti-BIN1 (BIN1 99D, 05-449, Millipore, 1/2500, ab27796, abcam, 1/1000), anti-α-tubuline (α-tubuline DM1A ...
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bioRxiv - Cancer Biology 2020Quote: ... Blocking solution (10% goat serum in PBS with 0.25% 100X triton) was added for 30 min followed by anti-vinculin antibody (Millipore, MAB3574) diluted in blocking solution at 1:300 ...
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bioRxiv - Biophysics 2020Quote: ... and 10 μg/ml anti-E-cadherin blocking antibody that targeted the extracellular domain of E-cadherin (Gumbiner, Stevenson and Grimaldi, 1988) (uvomorulin, monoclonal anti-E-cadherin antibody, Sigma); after incubation ...
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bioRxiv - Animal Behavior and Cognition 2020Quote: ... Sections were stained for NeuN immunohistochemistry using 1:10,000 polyclonal rabbit anti-NeuN antibody (ABN78; EMD Millipore; Temecula, CA, USA) and horse anti-rabbit secondary antibody via an HRP reagent (ImmPRESS MP-7401 ...
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bioRxiv - Biochemistry 2021Quote: ... the membranes were probed with a 1:10,000 dilution of alkaline-phosphatase-conjugated goat anti-mouse IgG secondary antibody (Sigma-Aldrich) in PBST ...
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bioRxiv - Biochemistry 2021Quote: Antibodies used for Western Blot include mouse monoclonal anti β-actin (clone AC-15, Sigma, St. Louis, MO, United States) at 0.13 μg/ml ...
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bioRxiv - Neuroscience 2021Quote: ... blocked in PBS containing 0.3% Triton X-100 and 10% normal donkey serum and incubated with primary antibody overnight at 4 °C overnight (Rockland, rabbit anti RFP, 1:500; Millipore, chicken anti-GFAP ...
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bioRxiv - Genetics 2021Quote: ... were washed with 1 ml PBS and incubated with 1 ml PBS and 0.1 μg monoclonal anti-FLAG antibody (Sigma F1804) per μL of dynabeads at 4 °C and constant rotation overnight ...
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bioRxiv - Cell Biology 2021Quote: ... plates were washed 4 times with PBST and further incubated with 100 μL per well of goat anti-human IgG (Fc specific)-Peroxidase antibody (1: 5000 dilution, Sigma) for 1 h at 37°C ...
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bioRxiv - Neuroscience 2021Quote: ... Slices were transferred to a solution containing anti-Vesicular Glutamate Transporter 2 (VGlut2) polyclonal guinea-pig antibody (AB2251-I Sigma Millipore) in a 1:2000 dilution and kept overnight at 4 °C ...
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bioRxiv - Immunology 2021Quote: ... 1×106 yeast cells were incubated in staining buffer (phosphate buffered saline + 0.5% BSA + 2mM EDTA) containing anti-Flag fluorescein isothiocyanate antibody (2 μg/mL; clone M2, Sigma-Aldrich) and the probes for 30 minutes at RT in darkness prior to washing 2 times in ice cold staining buffer ...
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bioRxiv - Neuroscience 2021Quote: ... Cells were blocked with 5 % BSA/PBS for 1 h before overnight incubation with primary antibodies in 1 % BSA/PBS (RAB5 21435 1:200 Cell Signalling and anti-Integrin-β1 MAB1997 1:1000, Millipore) at 4°C prior to washing and incubation with secondary antibodies (anti-rabbit AlexaFluor-546 [A11-35] and anti-mouse AlexaFluor-633 [A21052] Thermofisher ...
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bioRxiv - Developmental Biology 2020Quote: ... The following antibodies at the indicated dilutions were employed in western blot analysis: rabbit polyclonal anti HA (SIGMA, 1:3000), mouse monoclonal anti HA (Cell Signalling ...
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bioRxiv - Biophysics 2020Quote: ... The 3F6H-fused ZTL variants were precipitated (at 4 °C for 10 minutes under dim light) with anti-FLAG antibody (Sigma), which was bound to Protein G-coupled magnetic beads (Dynabeads Protein G ...
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bioRxiv - Cell Biology 2020Quote: ... Membranes were blocked for 1 hour in 3% BSA-TBS and incubated overnight at 4°C with the following primary antibodies: anti-phosphotyrosine 4G10 (Millipore), anti-phosphorylated SYK (pY525/526 ...
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bioRxiv - Cell Biology 2020Quote: ... were washed with 100 μl worm protein lysis buffer and incubated with monoclonal M2 mouse anti-Flag antibody (Sigma, F3165) or monoclonal rabbit anti-c-Myc antibody (Sigma ...
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bioRxiv - Biochemistry 2020Quote: ... Samples corresponding to 1.4 OD600 of cells were resolved over SDS-Western blot was developed using anti-His specific antibody (Cat no. Sigma:H1029) at a dilution of 1:3000 and HRP conjugated anti-mouse secondary antibody (Anti mouse IgG-A4416 ...
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bioRxiv - Biophysics 2021Quote: ... A Western blot was performed on aliquots from the supernatant and pellet using monoclonal anti-polyHistidine−alkaline phosphatase antibody (Sigma). Blots were images on a Typhoon FLA 9500 and band intensities were quantified using ImageQuant (Cytiva) ...
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bioRxiv - Evolutionary Biology 2020Quote: ... followed by incubation with a secondary Cy3-labeled polyclonal goat anti-rabbit antibody (AP132C, 1:800, Merck Millipore, Burlington, USA). Cells were mounted on slides using Fluoroshield™ with DAPI mounting medium (Sigma-Aldrich ...
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bioRxiv - Microbiology 2020Quote: ... diluted 1:1000 in phosphate-buffered saline (PBS) containing 0.05% Tween 20 (PBS-T) followed by anti-rabbit horseradish peroxidase (HRP)-coupled antibody (Sigma-Aldrich) diluted 1:1000 in PBS-T ...
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bioRxiv - Molecular Biology 2021Quote: ... Single cells from GFP positive pool were cultured for three to four weeks and colonies were screened for knockouts by western blotting using the anti-p31comet antibody (Millipore-Sigma). The guide RNA sequences targeting CHAMP1 in this study were ...
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bioRxiv - Neuroscience 2020Quote: ... The mouse anti-GFP-20 antibody was used in GRASP and synaptotagmin experiments (1:100 in PBST-NGS; Sigma-Aldrich); the mouse nc82 antibody was used in synaptotagmin::GFP and DenMark experiments ...
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bioRxiv - Microbiology 2020Quote: rAkata EBV-infected cells was reactivated at 1×106 cells/mL with a goat polyclonal anti-human IgG Fc-specific antibody (Sigma) for 48 hours ...
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bioRxiv - Microbiology 2020Quote: ... The permeabilized cells were washed with PBS and sequentially incubated anti-DENV primary antibody (dilution 1:400 of Mab8705, Millipore) and anti-Mouse Alexa-488 conjugated secondary antibody (dilution 1:500 ...
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bioRxiv - Immunology 2021Quote: ... All primary antibodies were purchased from Cell Signalling Technology (Singapore) except for rabbit anti-MLC phospho-Ser19 (Sigma Aldrich, Singapore), rabbit monoclonal anti-detyrosinated tubulin and phospho-tubulin Tyr272 (Abcam ...
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bioRxiv - Microbiology 2021Quote: ... milk powder in TBS-T (Tris-buffered saline-Tween-20) and then incubated overnight with primary antibody (monoclonal anti-FLAG, 1:1,000; Sigma-Aldrich, #F1804-1MG ...
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bioRxiv - Immunology 2020Quote: ... The sections were blocked in PBS containing 1% bovine serum albumin (BSA) for 1 h at RT and stained in blocking buffer containing primary antibody (anti-PP6C, Merck Millipore cat ...
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bioRxiv - Neuroscience 2020Quote: ... followed by 2h incubation with Alexa Fluor-488 goat anti-rabbit IgG secondary antibody (1:500, A11008, Merck Millipore, MA). Slices were transferred to glass slides and covered with Fluoromount (Sigma-Aldrich ...
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bioRxiv - Genetics 2020Quote: ... The expression of tagged MCM2 in HeLa and Patski cells was confirmed by Western blot using anti-MCM2 rabbit polyclonal antibody (HPA031495 Sigma) at 1:1,000 dilution.
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bioRxiv - Neuroscience 2020Quote: ... Slides were placed in a light-protected humidity-controlled tray and incubated in primary antibody (mouse-anti-Neurofilament 200; clone N52; Sigma) at 1:500 in blocking buffer overnight at 4°C ...
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bioRxiv - Biochemistry 2020Quote: ... the membrane was washed and incubated with primary antibody diluted in 5% BSA/TBST (1:10,000 anti-Flag (Sigma-Aldrich) or anti-Beta lactamase (Abcam) ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... washed and incubated with HRP-conjugated goat anti-rabbit secondary antibody and developed using enhanced chemiluminescence substrate (Millipore, ON, Canada). Membrane was stripped using 0.1 N NaOH and reprobed with rabbit anti-beta actin antibody (1:10,000 ...
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bioRxiv - Cell Biology 2021Quote: ... The following antibodies were used for immunoblotting: The HA epitope was recognised with a primary mouse monoclonal anti-HA (1:1000; Sigma); the Myc epitope was recognised with a primary mouse monoclonal anti-Myc (1:5000 ...
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bioRxiv - Neuroscience 2020Quote: ... Sections were then blocked in 5% NGS with 0.1% Triton X-100 and incubated with anti-NECAB1 rabbit antibody (Sigma Millipore #HPA023629) and anti-NeuN (Thermofisher Scientific #14H6L24 ...
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bioRxiv - Cell Biology 2020Quote: ... We incubated the membranes overnight at 4°C in constant agitation with anti-Hsp23 antibody (1:1000) (Sigma-Aldrich S0821), anti-Hsp26 (1:1000 ...
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bioRxiv - Cell Biology 2020Quote: ... We incubated the membranes overnight at 4°C in constant agitation with anti-Hsp23 antibody (1:1000) (Sigma-Aldrich S0821), anti-Hsp26 (1:1000 ...
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bioRxiv - Neuroscience 2022Quote: ... Protein were transferred to polyvinylidene fluoride membranes and immunoblotted with monoclonal antibodies at ratio of 1:1000 (anti-N1, Millipore, MAB1586 ...
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bioRxiv - Neuroscience 2022Quote: ... 1-2 mg protein lysate in 750 µl SLB buffer with protease and phosphatase inhibitors was incubated with 2.5-5 µg of anti-Flag antibody (Sigma, # F3165) for 2 hours at 4°C ...
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bioRxiv - Developmental Biology 2022Quote: ... Sections were washed and incubated overnight at 4°C with anti-Digoxigenin-AP antibody (45-11093274910 Merck-SIGMA, 1:1000). Staining was achieved by adding a solution of 4-Nitro blue tetrazolium chloride (NBT ...
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bioRxiv - Immunology 2022Quote: ... and the secondary antibody goat anti-mouse IgG (H+L) conjugated to Alexa Fluor 488 were obtained from Millipore Sigma. Monoclonal Rac1 antibodies (Cat ...
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bioRxiv - Neuroscience 2022Quote: ... 0.4% Triton and the rabbit anti-calbindin-D-28K primary antibody (C7354; Sigma-Aldrich St. Louis, MO, USA; 1:1000). Sections were subsequently washed in PBS ...
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bioRxiv - Molecular Biology 2022Quote: ... 250 μg of total protein samples were made up to 500 μl total volume with 1% NP-40 buffer and incubated overnight at 4°C with 1 μl of mouse anti-Flag primary antibody (#F1804; Sigma). Subsequently ...