Labshake search
Citations for Millipore Sigma :
6951 - 7000 of 10000+ citations for QuantiChrom β N Acetylglucosaminidase Assay Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2023Quote: ... male and female Il10−/− mice (n = 7 per sex) were switched to a diet containing 100 ppm of piroxicam (Sigma) (Envigo ...
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bioRxiv - Neuroscience 2023Quote: ... or vehicle comprised of 10% dimethyl sulfoxide (DMSO) in saline (n = 20) all acquired from Sigma Aldrich (St. Louis, Missouri). On the eighth day ...
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bioRxiv - Microbiology 2023Quote: ... 22 mm x 22 mm #1.5 borosilicate coverslips (DOT Scientific) were placed in a Wash-N-Dry™ coverslip rack (Sigma) and submerged in ∼80 mL of 1 M NaOH in a 100 mL glass beaker ...
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bioRxiv - Molecular Biology 2023Quote: Cys ox-PTMs were labeled using a bioswitch workflow (Fig. 1A and S1A) involving a thiol alkylation step using 2.3mM N-ethylmaleimide (NEM, Sigma Aldrich), followed by reduction of reversibly oxidized thiols using 4mM tris(2-carboxyethyl ...
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bioRxiv - Neuroscience 2023Quote: ... HEK cells were washed three times with ice-cold PBSx1 and lysed using Maltoside lysis buffer [1% N-dodecyl maltoside (cat no. 69227-93-6 – Sigma) dissolved in PBSx1 with freshly added 0.1 mg/ml PMSF and protease inhibitors] ...
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bioRxiv - Microbiology 2023Quote: ... Approximately 1 mL nitrate stock solution (10 g N L-1) containing ∼1% 15N-labelled sodium nitrate (98 atom % 15N, Sigma) was added to achieve a concentration of ∼20 mg N L-1 ...
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bioRxiv - Microbiology 2023Quote: ... followed by an introduction of 0.12 mL nitrate stock solution (40 g N L-1) which contained ∼1% 15N-labelled NO3- (98 atom % 15N, Sigma). The butane culture was flushed with argon gas (99.99% ...
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bioRxiv - Cell Biology 2023Quote: ... WM983B cells were infected by lentivirus in the presence of 5µg/mL polybrene (ref. n°TR-1003, Merck/Sigma-Aldrich) followed by antibiotic selection (puromycin 1µg/mL ...
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bioRxiv - Cell Biology 2023Quote: The C19orf43.WT plasmid is comprised of an N-terminal 3x Flag tag followed by human full length C19orf43 cloned into the p3XFlag-CMV-10 vector (Sigma). C19orf43.R7G was obtained by mutating the arginine at position 7 to glycine and C19orf43.R106G was obtained by mutating the arginine at position 106 to glycine ...
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bioRxiv - Biochemistry 2023Quote: ... was fused to a TEV-cleavable N-terminal His6-GCN4 tag and cloned into a pRSF-Duet1 vector (Merck Millipore). The KASH domain sequence of human JAW1/LRMP (amino-acids 515-555 and 531-555 for KASH6 and KASH6 core ...
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bioRxiv - Neuroscience 2023Quote: ... Tissues were dehydrated with methanol/PBS and incubated O/N at RT in 66% dichloromethane (DCM; cat. #: 270947; Sigma-Aldrich) and 33% methanol ...
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bioRxiv - Developmental Biology 2023Quote: The testes from 8-week-old C57BL/6J wild-type mice or 4-month-old Foxc2f/+ and Foxc2f/-;Ddx4Cre/+ mice (n=4) were minced and digested in the collagenase type IV (1 mg/mL, Sigma) and DNase I (500μg/mL ...
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bioRxiv - Bioengineering 2023Quote: ... Precusor nCys- mEpoR peptide was labeled at its free amino N-terminus as a protected peptide on resin with NHS-Biotin (Sigma) by swelling the resin with DMF having 10 equivalents N,N- Diisopropylethylamine ...
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bioRxiv - Biochemistry 2023Quote: ... Purified Mpro with authentic N- and C-termini was concentrated by a 10 kDa MWCO Amicon Ultra 50 centrifugal filters (Merck Millipore) at 4 °C to ∼ 10 mg/ml ...
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bioRxiv - Bioengineering 2022Quote: ... 3 mM aniline dimer solution was freshly prepared by dissolving 5.5 mg of N-phenyl-p-phenylenediamine (Sigma-Aldrich, 241393) in 10 mL of Tyrode’s solution ∼20 h at room temperature ...
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bioRxiv - Biochemistry 2022Quote: ... N-termini were labeled with light and heavy formaldehyde (12CH2O in H2O, and13CD2O in D2O (252549 and 596388, Sigma-Aldrich)) ...
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bioRxiv - Developmental Biology 2023Quote: Right tibias from SMAD1/5WT and SMAD1/5iΔEC mice (n= 10 per group) were collected and fixed in 4% paraformaldehyde (PFA; Sigma Aldrich) in PBS overnight (12h ...
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bioRxiv - Developmental Biology 2022Quote: ... the dish was filled with embryo water supplemented with MS222 (final concentration 160ng/ml) and N-Phenylthiourea (PTU) (0.045% stock-Sigma Aldrich) which was circulated with a peristalsis pump (Harvard Apparatus) ...
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bioRxiv - Microbiology 2023Quote: ... Hemin (0.5 mL/L of a 10 mg/mL stock which was prepared in 0.1 N NaOH; Sigma, Cat. # 51280). All solutions were made in Milli-Q-water unless otherwise noted ...
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bioRxiv - Biochemistry 2023Quote: ... 100 ul of reaction was passed over a Dowex cation exchange resin column (N-methyl D-glucamine form) (217484; Millipore-Sigma) to remove any uninternalized 14C-lysine ...
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bioRxiv - Biochemistry 2023Quote: ... where Cue1(24-203) (pAS153) and Ubc7 (pAS159) were expressed with an N-terminal His6 tag in a pET28B vector (Novagen). Cue1 and Ubc7 were also expressed from a K27-His14-pSUMO vector allowing for the complete removal of affinity and solubility tags55 ...
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bioRxiv - Plant Biology 2023Quote: ... 8-day-old plants (n=15) were dipped for 8 minutes in Lugol staining solution (0.1% I, 1%KI, Sigma-Aldrich) and observed in an Eclipse E200 Microscope (Nikon ...
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bioRxiv - Developmental Biology 2023Quote: ... mTeSR1 media was supplemented with 100 nM SiR-actin (to maintain strong actin fluorescence) and 2.5 mM N-acetyl-L-cysteine (NAC, Sigma-Aldrich A9165 ...
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bioRxiv - Cell Biology 2023Quote: ... and rotated at 4°C for 45 minutes in Hank’s Buffered Saline Solution with 10 mM EDTA and 1 mM N-Acetyl-L-cysteine (Sigma A9165). Crypts were isolated by scraping with a glass coverslip followed by vortexing and filtering through a 70 uM (small intestine ...
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bioRxiv - Microbiology 2023Quote: ... either alone or in the presence of 22 μL-enzymes cocktail constituted by 1U Cellulase (product n° 22178, Millipore-Sigma), 1U Beta-glucosidase (product n° 49290 ...
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bioRxiv - Microbiology 2023Quote: ... either alone or in the presence of 22 μL-enzymes cocktail constituted by 1U Cellulase (product n° 22178, Millipore-Sigma), 1U Beta-glucosidase (product n° 49290 ...
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bioRxiv - Neuroscience 2023Quote: ... were placed in the middle of a 2% agar plate containing a container with 10 µl n-amylacetate (AM, diluted 1:50 in mineral oil; SAFC) or 3-Octanol (3-Oct, Sigma) on one side and a blank on the other side ...
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bioRxiv - Neuroscience 2023Quote: ... or acidic pH 6.5 media (26mM NaHCO3, 20mM 2-(N-morpholino) ethanesulfonic acid (MES)(Millipore Sigma, St. Louis, MO, USA), 5mM HEPES ...
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bioRxiv - Immunology 2023Quote: ... were plated one day prior to the coculture with γδ T cells at a density of 15.000 cells/well in a flat-bottom 96-well plate and treated O/N with 10 μM pamidronate (PAM, Sigma) in complete IMDM or DMEM ...
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bioRxiv - Biochemistry 2024Quote: ... cells were sorted into wells containing 1μL of master mix (0.2% n-dodecyl-beta-maltoside (D4641-500MG, Sigma Aldrich, Germany), 100mM tetraethylammonium bicarbonate (17902-500ML ...
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bioRxiv - Microbiology 2023Quote: ... Human neuroblastoma cell line SK-N-SH (ATCC HTB-11) was cultured in Dulbecco’s Modified Eagle’s Medium with 1g/L glucose (Sigma-Aldrich, FIN) supplied with 10 % FBS ...
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bioRxiv - Biophysics 2023Quote: ... N’-tetramethylethane-1,2-diamine (TEMED) and 1% (v/v) of solution of 10% (w/v) ammonium persulfate (APS) (Sigma-Aldrich) was added to the mixture and vortexed to initiate the polymerisation ...
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bioRxiv - Bioengineering 2023Quote: ... sulfo-succinimidyl-4-(N-maleimidomethyl)cyclohexane-l-carboxylate (sulfo-SMCC, Sigma, at a concentration of 2 mg/mL in PBS) were added to the emulsion and kept to react for 1 h at room temperature ...
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bioRxiv - Cell Biology 2023Quote: ... cells were pelleted after 6 h or 12 h and resuspended in SD-N containing 30 mg/l L-lysine-13C6,15N2 hydrochloride (Lys8, Sigma, 608041) and incubated for indicated time ...
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bioRxiv - Bioengineering 2023Quote: ... diametrically-halved constructs (n=3) were stained over the exposed cross section with a speckled pattern of 1% hematoxylin (Sigma) via airbrush ...
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bioRxiv - Biochemistry 2023Quote: Expression of myrArf1 proteins with C-terminal 6x His tags was accomplished via co-expression with yeast N-myristoyltransferase (yNMT) using the pETDuet-1 vector (Novagen) in E ...
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bioRxiv - Cell Biology 2023Quote: ... N-terminal peptide enrichment was performed on the digested sample by removing the internal tryptic peptides with undecanal (Sigma, U2202). Enriched N-terminal peptides were then desalted with a C18 spin column (Thermo Fisher Scientific ...
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bioRxiv - Microbiology 2024Quote: ... In the reciprocal experiment 1 mg of N-terminally his-tagged Saci_1104 was loaded onto a Nickel-NTA (Sigma, Germany) column (column volume of 250 µl) ...
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bioRxiv - Biochemistry 2024Quote: ... coli including a polyhistidine tag and thrombin cleavage site at its N-terminus was amplified via PCR and subcloned into pET-30b (Novagen) between the NdeI/ BamHI sites (Vermot et al. ...
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bioRxiv - Cell Biology 2024Quote: Tubulin was purified from porcine brain using established protocols as described previously [39] Polarity-marked microtubules were prepared by preferably extending the plus-ends of long GMPCPP seeds in the presence of N-ethylmaleimide (Sigma) modified tubulin (NEM-tubulin) ...
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bioRxiv - Cell Biology 2024Quote: ... Two µl of colorless hemolymph was aspirated from each pile and separately transferred to 96-well plates (Thermo-Scientific) containing 0.1% N-Phenylthiourea (Sigma-Aldrich) in 50Lµl PBS ...
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bioRxiv - Neuroscience 2024Quote: Infant (n=1) and adult (n=1) hippocampal sections (10μm) were fixed in 10% neutral buffered formalin (catalog no. HT501128, Sigma-Aldrich) for 30 minutes at RT ...
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bioRxiv - Microbiology 2024Quote: ... The resultant protein with an N-terminal His6-tag was concentrated using a 10 kDa Amicon Ultra-15 concentrator (Millipore). Protein concentrations were determined from the absorbance at 280 nm using a molar absorptivity constant (ε280 = 22,920 M-1 cm-1 ...
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bioRxiv - Bioengineering 2024Quote: ... The mixture was neutralized with 1 N acetic acid until gas evolution ceased and filtered through Celite 545 (EMD Millipore). The solution was concentrated by rotary evaporation at 50 °C and precipitated in cold diethyl ether (Fisher Scientific ...
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bioRxiv - Bioengineering 2024Quote: ... A 1 wt% alginate solution was prepared in 0.1 M 2-(N-morpholino)ethanesulfonic acid buffer (MES, Sigma Aldrich, M8250). Sulfonated N-hydroxysuccinimide (NHS ...
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bioRxiv - Biochemistry 2024Quote: ... as a Tobacco Etch Virus (TEV)-cleavable N-terminal His-tagged fusion protein using a pET46 Ek/LIC vector (Novagen) and purified using Ni-NTA affinity chromatography and gel filtration chromatography ...
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bioRxiv - Biochemistry 2024Quote: N-Lactoyl Phenylalanine (catalogue # 318943) was purchased from Novo Pro Labs and 1-Carboxyethylphenylalanine (ENAH95E73EBB) was purchased from Sigma Aldrich.
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β-1,6-glucan plays a central role in the structure and remodeling of the bilaminate fungal cell wallbioRxiv - Microbiology 2024Quote: ... were identified and quantified by gas liquid chromatography (Perichrom) as their alditol acetates obtained after hydrolysis (4 N trifluoroacetic acid (TFA, Sigma), 100 °C ...
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bioRxiv - Immunology 2024Quote: ... followed by activation with 50 mM 3-Dimethylamino-propyl)-ethyl-carbodiimide Hydrochloride (EDC)/100 mM N-Hydroxysuccinimide (NHS) (Sigma-Aldrich) for 12 min at 10 ml/min ...
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bioRxiv - Immunology 2024Quote: HAP1 target cell lines were plated one day prior to the coculture with γδ T cells at a density of 15,000 cells/well in a flat-bottom 96-well plate and treated O/N or not with 10 µM pamidronate (PAM, Sigma) in complete IMDM (includes 10% FCS ...