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Citations for Millipore Sigma :
7151 - 7200 of 10000+ citations for Anti CD152 Antibody since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2020Quote: ... The proteins in both soluble fraction and membrane fraction were separated in 12% SDS-PAGE gel and analysed by western blotting using anti-GFP polyclonal antibody (1:4000; Sigma-Aldrich).
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bioRxiv - Cell Biology 2020Quote: ... was then added to cells for 20 minutes and subsequently replaced with an anti-FLAG antibody (1:1000 in blocking solution) (Cat #F3165, Sigma-Aldrich) for one hour ...
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bioRxiv - Cell Biology 2019Quote: ... Samples were blocked for at least 2.5 hours at room temperature followed by the addition of poly-clonal rabbit anti-histone H4 (citrulline 3) antibody (EMD Millipore) used at 1:100 and incubated overnight at 4°C ...
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bioRxiv - Cell Biology 2019Quote: ... A polyclonal rabbit anti-AGPAT (SAB2700797) and a monoacylglycerol lipase An MAGL rabbit polyclonal antibody (Cat. 011994) were purchased from Sigma-Aldrich. A rabbit anti-ATGL (mAb ab207799 ...
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bioRxiv - Cell Biology 2020Quote: ... The membrane was washed four times (each for 10 min) in TBS-T before being incubated with an anti-mouse horse radish peroxidase (HRP)-conjugated secondary antibody (A9044, Sigma-Aldrich) or an anti-rabbit HRP-conjugated secondary antibody (31466 ...
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bioRxiv - Cell Biology 2020Quote: Lamin A proteins were detected by probing with mouse anti-Myc antibodies (clone 4A6, Millipore cat #05-724; 1:10,000 dilution) decorated with goat anti-mouse secondary IRDye 680RD antibodies (LI-COR ...
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bioRxiv - Developmental Biology 2021Quote: ... samples were incubated overnight at 4°C on a rocking platform with the primary antibody anti-H3K9ac (1:1,000; Sigma, cat. # H9286) or anti-H3K9bhb (1:1,000 ...
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bioRxiv - Developmental Biology 2021Quote: Innervation analysis was done on regenerating limb and flank sections to quantify innervation abundance using the Mouse Monoclonal Anti-Acetylated Tubulin antibody (1:200 dilution – Sigma Aldrich), followed by the Goat-Anti-Mouse IgG Alexa Fluor 488 (1:200 dilution – Abcam ...
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bioRxiv - Developmental Biology 2020Quote: ... the blot was washed in PBST and incubated in primary antibodies of rabbit polyclonal anti-TBX5 at a 1:400 dilution (Sigma #HPA008786) and mouse monoclonal anti-cTNT at 1:1000 dilution (ThermoFisher Scientific #MS-295-P) ...
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bioRxiv - Molecular Biology 2021Quote: ... 10 % lysate was saved as input and IP was performed using an anti-pan-AGO antibody (clone 2A8, MABE56 Sigma-Aldrich) and an anti-FLAG M2 antibody (F3165 ...
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bioRxiv - Developmental Biology 2021Quote: ... followed by immunocytochemistry with mouse monoclonal anti-vinculin antibody (1:200 dilution in 1% BSA–PBS) (Sigma; V9264, clone hVIN-1) and Alexa 555–labelled goat anti–mouse IgG (#A21422 ...
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bioRxiv - Developmental Biology 2021Quote: Analysis of nerve development in E10.5 embryos was performed using a monoclonal anti-neurofilament 160 (NF160) antibody (#N5264, Sigma Aldrich, USA) as previously described (Clouthier et al. ...
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bioRxiv - Microbiology 2020Quote: ... The cells were then incubated for 1hr with primary antibodies diluted as follows with Block buffer: mouse monoclonal anti-FLAG (Sigma Aldrich) at 1:500 ...
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bioRxiv - Molecular Biology 2022Quote: ... GPR126 and empty vector-transfected cells were stimulated with the indicated concentrations of anti-HA antibody (H3663, stock concentration 1 mg/ml, Sigma-Aldrich), anti-HA Fab fragment (stock concentration 1 mg/ml ...
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bioRxiv - Molecular Biology 2022Quote: Tipless silicon nitride AFM cantilevers (NanoWorld, PNP-TR-TL) were coated with monoclonal anti-HA antibodies produced in mouse (H3663, Sigma-Aldrich) or Fc-control for the antibody-based mechano-activation experiments using flexible PEG spacers as described before (Ebner et al. ...
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bioRxiv - Molecular Biology 2022Quote: ... followed by incubation with 3-isobutylmethyl-xanthine (1 mM)-containing medium or including the secondary antibody anti-mouse IgG (Fc specific, M2650, Sigma-Aldrich) for 30 min ...
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bioRxiv - Neuroscience 2022Quote: ... retinas were incubated again in blocking solution with primary antibody against choline acetyltransferase (ChAT; Millipore, AB144P, goat anti-ChAT, 1:1000) for 5 nights at 4°C ...
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bioRxiv - Neuroscience 2021Quote: ... We use a combination of antibodies (rabbit anti-parvalbumin, 1:5000, PV235, Swant + biotin-conjugated lectin from WFA, 1:250, L-1516, Sigma-Aldrich). On the day after ...
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bioRxiv - Neuroscience 2021Quote: ... for 20min and rescanned to ensure loss of signal before being incubated with an anti-GAFPH antibody (Sigma, 45-G9545-100UL) as a loading control ...
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bioRxiv - Neuroscience 2021Quote: ... washed as above described and re-blotted using mouse anti-β-actin antibody (Sigma-Aldrich, USA; 1:2500, 1 h, RT). Again ...
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bioRxiv - Evolutionary Biology 2022Quote: ... three technical replicates for each sample were analyzed by SDS-PAGE and Western blot using Anti-FLAG® antibody (Sigma-Aldrich Monoclonal ANTIFLAG® M2-Peroxidase (HRP ...
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bioRxiv - Genomics 2019Quote: ... GFP+ cells were FACS sorted and expression of the RRM-FLAG construct was confirmed by Western Blot using an anti-FLAG antibody (Sigma F3165). SpenRRM-FLAG expressing cells were treated with 10 mM HDAC1/2 inhibitor (Cayman Chemical Company ...
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bioRxiv - Neuroscience 2019Quote: ... blocked using 1% BSA in TBST and incubated with primary anti-TH antibody (dilution 1:500; AB152, Merck Millipore, Schaffhausen, Switzerland). 24h later ...
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bioRxiv - Physiology 2020Quote: ... Proteins were detected after incubation of the membranes with secondary GP anti-rabbit alkaline phosphatase-conjugated antibody (1:30000, cat# A3687, Sigma Aldrich), GP anti-mouse alkaline phosphatase-conjugated antibody (1:20000 ...
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bioRxiv - Cell Biology 2019Quote: ... anti-GFP (in house made rabbit polyclonal antibody; 1:1,000-4,000) anti-Vinculin (mouse monoclonal; clone hVIN-1; Sigma-Aldrich; 1:10000), anti PMF1/NNF1 (in house made mouse affinity purified monoclonal ...
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bioRxiv - Immunology 2019Quote: ... cells were washed twice with PBS for 1 h at 37°C and then incubated with a FITC-conjugated rabbit anti-mouse IgG antibody (Sigma-Aldrich) at a 1:500 dilution ...
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bioRxiv - Molecular Biology 2019Quote: 90 µg of MRC5 chromatin extracts were incubated overnight at 4° C in 150 μl total volume of IP buffer (see above) with antibody (RNAP1: anti-RPA194, sc-48385, Santa Cruz; NMI: M3567 Sigma) (ChIP ...
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bioRxiv - Molecular Biology 2019Quote: ... solution and incubated for 1.5 h RT or overnight at 4° C with the primary antibodies in milk PBS-T (RNAP1: anti-RPA194, sc-48385, Santa Cruz; ACTβ: A5316, Sigma or ab8227, Abcam; NMI: M3567, Sigma; α-Tubulin ...
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bioRxiv - Molecular Biology 2019Quote: ... solution and incubated for 1.5 h RT or overnight at 4° C with the primary antibodies in milk PBS-T (RNAP1: anti-RPA194, sc-48385, Santa Cruz; ACTβ: A5316, Sigma or ab8227 ...
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bioRxiv - Genetics 2019Quote: ... cells on coverslips were fixed in 4% PFA for 20 min and blocked with 3% BSA in PBS for 10 min before applying Ret-P1 anti-rhodopsin antibody (Sigma #O4886) at a final concentration of 1:1000 in blocking buffer for 1 hr ...
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bioRxiv - Immunology 2020Quote: ... nitrocellulose membranes were probed with protein-specific primary antibodies diluted in PBS containing 5% Milk or 5% BSA as follows: anti-actin Cat #A5441 from Sigma Aldrich; anti-tubulin Cat #5286 from Santa Cruz ...
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bioRxiv - Cancer Biology 2019Quote: ... the TMA slide was subjected to Cell Conditioning 1 (CC1, Ventana) antigen retrieval and stained with a prediluted anti-MCT antibody (Mouse, Clone G3, Millipore #MAB1222) using an automated immunostainer ...
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bioRxiv - Genetics 2019Quote: Cell extracts were prepared by TCA precipitation and proteins were separated by 10% SDS-PAGE followed by immunoblotting with anti-HA antibody (Sigma-Aldrich, ref ...
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bioRxiv - Molecular Biology 2019Quote: ... NF-kB (CST, #3033), PCM1 (CST, #5213) and GABARAP (CST, #26632) and NDP52 (. Secondary antibodies: anti-Mouse IgG–Peroxidase (Sigma, A5906), anti-Rabbit IgG–Peroxidase (Sigma ...
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bioRxiv - Physiology 2020Quote: ... containing 5% w/v non-fat dry milk followed by anti-rabbit IgG HRP-conjugated goat secondary antibody (A0545, Sigma-Aldrich). Signal was detected using the SuperSignal West Dura Extended Duration Substrate (Thermo Fisher Scientific ...
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bioRxiv - Molecular Biology 2020Quote: ... 1% of pre-cleared chromatin was set aside for input and 5 µg Monoclonal ANTI-FLAG® M2 antibody (to capture FLAG-tagged dCas9) or IgG (Sigma) was added to the remaining volume and then incubated at 4°C on a nutator overnight ...
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bioRxiv - Cancer Biology 2019Quote: ... Immunoprecipitates were then washed three times with cold Triton lysis buffer and were analyzed by western blot using an anti-His antibody (Sigma Aldrich).
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bioRxiv - Microbiology 2019Quote: ... The plate was washed three times with wash buffer (PBS/0.1% Tween-20) and the primary antibody (anti-NP mouse monoclonal Ab, Millipore, cat. MAB2851) added in blocking buffer ...
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bioRxiv - Biophysics 2019Quote: ... the cells were incubated for 1 hour at 37°C with 1:300 rabbit anti-β-tubulin antibody (Sigma Aldrich, T5293) in PBS + 1% BSA ...
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bioRxiv - Biophysics 2019Quote: ... the cells were incubated for 1 hour at 37°C with 1:300 mouse anti-α-tubulin antibody (Sigma Aldrich, T6199) in PBS + 1% BSA ...
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bioRxiv - Cell Biology 2019Quote: ... Proteins were analyzed on 5-5.5 % SDS-PAGE gels followed by Western blotting with the anti-Flag M2 antibody (Sigma-Aldrich, USA).
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bioRxiv - Immunology 2019Quote: ... haemolysis assay was performed with sheep red blood cells (TCS Biosciences) sensitized with Anti-Sheep Red Blood Cell Stroma antibody (cat. no. S1389, Sigma-Aldrich). 50 μl cells (5 x 108 cells/ml ...
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bioRxiv - Microbiology 2019Quote: ... For antigen-antibody detection membranes were incubated for 1h at room temperature with a 1:2500 dilution of anti-rabbit IgG (whole molecule)— alkaline phosphatase antibody produced in goat (Sigma A0545) in PBS pH7.4 ...
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bioRxiv - Cell Biology 2019Quote: ... The RBM proteins were examined with the primary antibodies and the following secondary anti-rabbit IgG conjugated with Horse radish peroxidase (A6154; Sigma-Aldrich) or conjugated with alkaline phasphatase (T2180;Applied Biosystems) ...
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bioRxiv - Microbiology 2020Quote: ... Membrane was further blocked with 5% milk in 1X PBS and Tween 20 for 30 min followed by incubation with mouse anti-gE monoclonal antibody (clone 8612, EMD Millipore) (1:3,000 ...
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bioRxiv - Biophysics 2020Quote: ... a solution of 20 µg ml-1 biotinylated anti Mouse IgG antibody (#115-065-003-JIR-2ml STRATECH SCIENTIFIC LIMITED) and 20 µg ml-1 anti-Dynein Intermediate chain antibody (#MAB16818 Sigma-Aldrich). Unreacted streptavidin binding sites were then blocked using biotin-BSA (#PN29130 ...
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bioRxiv - Pharmacology and Toxicology 2019Quote: ... the infected cells were fixed with cold acetone and tested using CPV-2-specific canine antibodies and rabbit anti-dog IgG conjugated with fluorescein isothiocyanate (Sigma Aldrich). Viral titre ...
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bioRxiv - Neuroscience 2019Quote: ... Protein samples of PSD95 and Kal7 or PSD and Kal8 were incubated at 4oC overnight with rabbit anti-Flag Antibody (#F7425 Sigma; RRID:AB_439687) in a final volume of 120μl 20mM TES ...
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bioRxiv - Neuroscience 2019Quote: ... Sections were incubated overnight at 4°C with primary antibodies: goat anti-choline acetyltransferase (ChAT; 1:500; Millipore Cat# AB144P, RRID:AB_2079751), mouse anti-parvalbumin (PV ...
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bioRxiv - Neuroscience 2020Quote: ... The specimens were incubated at room temperature for 1 h in blocking buffer (PBSTX with 10% normal sheep serum and 2% bovine serum albumin) and subsequently with the primary antibody (rabbit-anti-GABA, Sigma-Aldrich A2052 ...