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Citations for Millipore Sigma :
7351 - 7400 of 10000+ citations for Anti CD152 Antibody since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2021Quote: ... Sections were incubated overnight at 4° C with the following primary antibodies in 1X PBS containing 2% Triton X-100 and 2% horse serum: mouse anti-NeuN (EMD Millipore), rat anti-Ki-67 (Invitrogen) ...
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bioRxiv - Neuroscience 2021Quote: ... Proteins were blocked in 5 % skimmed milk in PBS prior to incubation with primary antibody (anti-APP A8717 1:5000 Sigma Aldrich) at 4°C overnight prior to incubation with anti-rabbit HRP ...
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bioRxiv - Neuroscience 2020Quote: ... to preclear samples for 2 hours and subsequently incubated with Dynabeads protein G coated with an anti-Flag antibody (Sigma, F1804). The lysate-beads mixture was incubated at 4°C with rotation for 2 hours ...
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bioRxiv - Developmental Biology 2020Quote: CTCF was immunoprecipitated overnight at 4°C from 100 μg digested chromatin diluted in ChIP buffer (Jebeniani et al., 2016) using an anti-CTCF antibody (reference 07–729 Millipore France). The bound fraction was diluted and digested fragments ligated using T4-ligase (In vitrogen ...
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bioRxiv - Developmental Biology 2021Quote: ... then incubated with primary antibodies diluted in 5% milk overnight at 4°C (anti-ATG13 (SAB4200135, 1:100, Sigma, MI, USA); anti-GAPDH (10494-1-AP ...
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bioRxiv - Biophysics 2020Quote: ... The cells were then washed with 1× PBS 3 times and immunofluorescence staining was performed using mouse anti-alpha tubulin primary antibody (T5168, Sigma Aldrich) at 1:1000 dilution ...
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bioRxiv - Cell Biology 2020Quote: ... membranes were washed five times with TBS and incubated with secondary antibody (goat-anti-rabbit/mouse HRP-conjugated,1:2000 in TBS + 5% dry milk, Sigma Aldrich) for 45 minutes at RT ...
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bioRxiv - Microbiology 2021Quote: ... permeabilized with 0.2% Triton X-100 (in Phosphate Buffered Saline [PBS]) and subjected to an immunofluorescence assay (IFA) using a monoclonal antibody (MAb) anti-ACE2 (Sigma-Aldrich), and then incubated with a goat anti-rabbit IgG (goat anti-rabbit IgG ...
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bioRxiv - Molecular Biology 2021Quote: ... Single cells from GFP positive pool were cultured for three to four weeks and colonies were screened for knockouts by western blotting using the anti-p31comet antibody (Millipore-Sigma). The guide RNA sequences targeting CHAMP1 in this study were ...
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bioRxiv - Immunology 2021Quote: ... was used at a 1:5000 dilution (T84) or horseradish peroxidase-conjugated goat anti-rabbit IgG secondary antibody (A0545, Sigma-Aldrich) at 1:2000 dilution ...
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bioRxiv - Microbiology 2021Quote: ... 1% input and 100% of IP were used for SDS-PAGE and probed with anti-FLAG antibody (1:3000, Sigma F7425).
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bioRxiv - Immunology 2021Quote: ... and then monoclonal anti-6x(HIS)-tag antibody was probed followed by detection with anti-mouse IgG conjugated with alkaline phosphatase (A3562: 1ml Sigma, USA) was used for developing the blots.
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bioRxiv - Microbiology 2021Quote: ... was from Thermo Fisher Scientific (Massachusetts, USA). 3-MA (Cat no. M9281) and Anti-HA antibody (Cat no. A190-101) was from Sigma (Missouri, USA). Nontargeting siRNA (Cat no ...
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Targeting properdin - Structure and function of a novel family of tick-derived complement inhibitorsbioRxiv - Immunology 2021Quote: ... Assays for the classical and lectin pathways were performed with sheep red blood cells (TCS Biosciences) sensitized with anti-sheep red blood cell stroma antibody (cat. no. S1389, Sigma-Aldrich), alternative pathway assay was performed with rabbit red blood cells (TCS Biosciences) ...
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bioRxiv - Immunology 2020Quote: ... Mouse antibodies were detected by incubation with polyclonal goat anti-mouse IgG-horseradish peroxidase conjugate (Sigma-Aldrich, St. Louis, MO, USA). The plate was developed with TMB substrate (Thermo Fisher Scientific ...
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bioRxiv - Bioengineering 2021Quote: ... Equal amounts of protein were subject to SDS-PAGE and blotted with anti-Rho antibody (3 μg/mL, #05-778, EMD Millipore) or anti-GAPDH antibody (1:1000 dilution ...
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bioRxiv - Microbiology 2021Quote: ... Plates were washed and bound human ACE2 was detected using HRP-conjugated anti-Flag antibody (Sigma-Aldrich, A8592, 1:5,000 dilution) and TMB substrate ...
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bioRxiv - Microbiology 2021Quote: ... Spore suspensions were then incubated for 1□h with 1:20 donkey anti-mouse IgG antibody coupled to 12-nm gold particles (Sigma G7777) in PBS–1% BSA for 1□hr at RT ...
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bioRxiv - Immunology 2020Quote: ... were washed twice with 1 mL of buffer and sensitised with a 1/1000 dilution of rabbit anti-sheep red blood cell stroma antibody (Sigma; S1389). After a 30 °C / 30 minutes incubation ...
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bioRxiv - Immunology 2020Quote: ... The membrane was initially blocked with 5% skin milk with PBS buffer for 2 hours and after few PBS rinse the membrane was incubated with monoclonal Anti-polyHistidine−Peroxidase antibody produced in mouse (Sigma-Aldrich).
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bioRxiv - Genomics 2021Quote: ... The membranes were incubated overnight at 4°C with a 1:5000 dilution of monoclonal rabbit anti-HA antibodies (Sigma-Aldrich) or 1:5000 dilution of monoclonal rabbit anti-FLAG antibodies (Sigma-Aldrich).
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bioRxiv - Cell Biology 2021Quote: ... followed by a goat anti-rabbit secondary antibody conjugated with Cy3 for 1hr at room temperature (1:500, Millipore, Cat. #AP132C).
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bioRxiv - Developmental Biology 2021Quote: ... Embryos were blocked in %5 BSA for 2 h at room temperature and incubated with anti-desmin antibody (1:20; Sigma, D8281) for two overnights ...
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bioRxiv - Developmental Biology 2021Quote: ... blocked for one hour at room temperature (%5 nonfat dried milk in %0,2 TBS-T) and probed with anti-desmin antibody (1:1000; Sigma-Aldrich, D8281) overnight at 4°C or anti-lamin B1 antibody (Abcam ...
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bioRxiv - Immunology 2021Quote: ... Wells were incubated with serial dilutions of the serum samples and the bound total IgG specific antibodies were detected by Peroxidase-conjugated Goat Anti-Mouse IgG (Sigma-Aldrich). Finally ...
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bioRxiv - Cell Biology 2021Quote: ... Membranes were washed in 1xTBS and then in BS before probing with anti-rabbit antibody conjugated to Horse Radish Peroxidase (HRP) (1:10,000 in BS, Sigma, goat, A9169) for 60 minutes at RT ...
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bioRxiv - Genomics 2021Quote: Localization of resulting plasmids was assessed with immunofluorescence staining using an anti-flag antibody (F7425, Sigma Aldrich, Merck, Kenilworth, NJ, USA) in combination with Alexa 546 secondary antibody (Invitrogen ...
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bioRxiv - Genomics 2020Quote: ... permeabilized with 0.2% Triton X-100 in PBS and then stained with primary antibody specific for Cy3-conjugated anti-αSMA (1:400, Sigma, #C6198), rat anti-PDGFRβ (1:200 ...
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bioRxiv - Genomics 2020Quote: ... Incubation with monoclonal anti-ATPIF1 (ATP synthase subunit IF1) antibody diluted in staining buffer (PBS with 1% bovine serum albumin (BSA) (Sigma-Aldrich)) was performed overnight at 4°C ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: The freshly isolated vessels were fixed with 3.7% PFA and incubated with mixed primary antibodies of anti-α-SMA (1: 300, A2547, Sigma-Aldrich) and anti-CYP4A (1 ...
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bioRxiv - Molecular Biology 2020Quote: ... Blocking with 5% BSA in PBS for 1 hour at 37°C and incubation with an anti single-stranded DNA antibody (Sigma, MAB3034) diluted 1:80 in 5% BSA in PBS ...
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bioRxiv - Cell Biology 2021Quote: ... 500 µg of lysate proteins were incubated with 2 µl of anti-Flag monoclonal antibody pre-adsorbed on protein A-Sepharose beads (cat. no. A2220, Sigma-Aldrich) overnight at 4°C.
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bioRxiv - Neuroscience 2020Quote: ... Tissues were then incubated overnight with the following cocktail of primary antibodies to presynaptic components diluted in blocking solution with rocking: rabbit anti-NF 200 (1:200; Sigma, N4142), mouse anti-synaptophysin (1:300 ...
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bioRxiv - Microbiology 2022Quote: ... and incubated with goat anti-mouse antibody conjugated to alkaline phosphatase (Sigma-Aldrich; A3688-1 MI; 1:10,000 1% BSA/PBS) for 2 hr at RT ...
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bioRxiv - Neuroscience 2022Quote: ... and 0.3% Triton for one hour at room temperature prior to incubation with the primary antibody mouse anti-GAD65 (EMD Millipore, MAB3551) at a concentration of 1:100 overnight at 4°C ...
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bioRxiv - Neuroscience 2022Quote: ... The sections from the animals of different treatment groups were washed using PBS and permeabilized with 0.3% Triton X-100 and followed by incubation overnight at 4°C with the following primary antibodies:: rabbit anti-NeuN (1:100, Sigma-Aldrich), mouse anti-GFAP (1:500 ...
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bioRxiv - Molecular Biology 2022Quote: The following antibodies were used for ChIP against the indicated epitopes: anti-Rpb1 clone 8wG16 (mouse, monoclonal, Millipore # 05-952-I), anti-Rpb1-S2-P clone 3E10 (rat ...
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bioRxiv - Neuroscience 2022Quote: ... Sections were incubated overnight at 4°C in anti-NeuN primary antibody (1:5000; monoclonal-Mouse Cat# MAB377; Millipore, California USA) in PBSTx with 1% normal goat serum (NGS ...
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bioRxiv - Cancer Biology 2022Quote: ... and cells were then incubated for 90 minutes at RT with 0.1% Triton X-100 containing Alexa Fluor 647-conjugated anti-puromycin antibody (1:5000; Millipore, MABE343-AF647). Cells were washed thrice with PBST for 5 minutes each ...
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bioRxiv - Biophysics 2022Quote: ... FLAG- TOP3A-RMI1/2 or FLAG-DNA2 were labeled with QDs pre-incubated with a mouse anti-FLAG antibody (Sigma-Aldrich) on ice for 10 minutes prior to injection ...
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bioRxiv - Biophysics 2022Quote: ... the cells were incubated for 1 hour at 37°C with 1:300 mouse anti-α-tubulin antibody (Sigma Aldrich, T6199) in PBS + 1 % BSA ...
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bioRxiv - Biophysics 2022Quote: ... the cells were incubated for 1 hour at 37°C with 1:300 mouse anti-α-tubulin antibody (Sigma Aldrich, T6199) in PBS + 1 % BSA ...
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bioRxiv - Biophysics 2022Quote: ... cells were permeabilized with 0.3% Triton X-100 in PBS and then incubated with anti-α-actinin mouse monoclonal antibody (1:100, A7732, Sigma-Aldrich) in CAS-Block reagent (Life Technologies ...
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bioRxiv - Biophysics 2022Quote: GMPCPP-stabilized microtubule seeds polymerized from biotinylated and TAMRA-labeled bovine tubulin were attached to the surface of a microchannel using anti-biotin antibodies (Sigma-Aldrich). Microtubules were assembled using 12 μM unlabeled bovine GTP-tubulin for 10 min at 28 °C in polymerization buffer (80 mM K-PIPES ...
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bioRxiv - Cell Biology 2022Quote: ... The membrane was then incubated with primary antibodies overnight at 4°C: anti-Alpha Tubulin (1:200, DSHB 12G10) and β-catenin (1:10,000 dilution, Sigma ABE208). The membrane was washed three times using PBST ...
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bioRxiv - Cell Biology 2022Quote: ... Lysates collected were run in SDS-PAGE (30 μl loaded) and blotted with the following primary antibodies: mouse anti-Miro1 (WH0055288M1, Sigma-Aldrich) at 1:1,000 ...
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bioRxiv - Cell Biology 2022Quote: ... and then immunoblotted with the following primary antibodies in TBST with 5% milk at 4°C overnight: mouse anti-Miro1 (WH0055288M1, Sigma-Aldrich) at 1:1,000 ...
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bioRxiv - Genetics 2022Quote: ... TAP-tagged Rrp45 protein was detected with peroxidase anti-peroxidase (PAP) soluble complex antibody produced in rabbit (1:5000, Sigma-Aldrich). The 3-phosphoglycerate kinase (Pgk1 ...
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bioRxiv - Plant Biology 2022Quote: ... The membranes were washed with Tris buffered saline containing 0.1% Tween-20 and probed again for protein loading control using a 1:10000 dilution of mouse monoclonal anti-actin (plant) primary antibody (Sigma, A0480) and a 1:3000 dilution of goat anti-mouse IgG HRP conjugate (Biorad).
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bioRxiv - Microbiology 2022Quote: ... α-PilC 20 and α-RomY antibodies were used together with goat anti-rabbit immunoglobulin G conjugated with horseradish peroxidase (Sigma) as secondary antibody ...