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Citations for Millipore Sigma :
7001 - 7050 of 10000+ citations for Anti CD152 Antibody since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2024Quote: ... The cells were incubated with mouse anti-FLAG primary antibody (1:1000 dilution in PBS + 0.1% BSA; Sigma– Aldrich, F3165) at room temperature for 30 min and washed three times with PBS + 0.1% BSA ...
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bioRxiv - Cancer Biology 2024Quote: ... Protein extracts of puromycin pulsed and non-puromycin pulsed control cells were subject to immunoblot (as above) using anti-puromycin antibody (clone 12D10, 1:1000, Sigma).
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bioRxiv - Cell Biology 2024Quote: ... cultures were incubated for 1 h at room temperature with primary antibodies as follows: mouse anti-laminA/C (Sigma SAB4200236), mouse anti-phospho-histone H2AX (Merck 05-636) ...
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bioRxiv - Molecular Biology 2024Quote: Primary antibodies used in this study were: mouse anti-FLAG M2 (Sigma-Aldrich #F3165, RRID: AB_259529, 1:1000-1:2500), mouse anti-α-tubulin (Cell Signaling Technologies #3873 ...
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bioRxiv - Molecular Biology 2024Quote: ... 0.1% Tween-20 (TBS-T) and incubated for one hour at RT with Mouse anti-FLAG (M2) antibody-HRP conjugated (Sigma) diluted 1:4000 in blocking milk ...
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bioRxiv - Molecular Biology 2024Quote: ... the BG4 antibody67 was incubated for 1 hour at 37°C and subsequently incubated with 1:800 mouse anti-FLAG antibody (F1804, Sigma) to BG4 and 1:500 rabbit anti-PARP1 (ab227244 ...
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bioRxiv - Immunology 2024Quote: ... the plates were washed three times and a solution of anti-nucleoprotein antibodies (Sigma, #MAB8257 and #MAB8258, 1:4000 each) in antibody diluent were added for 1 hour at room temperature ...
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bioRxiv - Neuroscience 2024Quote: ... Tissues were then transferred to a ChAT primary and TrkA primary antibody solution with blocking buffer (1:200 dilution goat anti-ChAT polyclonal AB143, Millipore EMD ...
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bioRxiv - Neuroscience 2024Quote: ... followed the same staining and mounting protocol described above but were incubated ON with a dilution of 1/2,000 anti-HA rabbit antibody (Sigma; H6908), and a 1/1000 dilution of AlexaFluor-488 donkey anti-chicken (Jackson InmunoResearch ...
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bioRxiv - Neuroscience 2024Quote: ... Sections were incubated overnight at 4 °C with antibodies that recognise specific proteins expressed by each cell type such as mouse anti-NeuN (1:250; Millipore) to label neurons ...
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bioRxiv - Cell Biology 2024Quote: Antibodies that were used in this study to detect viral and cellular proteins were the monoclonal antibody (MAb) recognizing the FLAG-tag epitope anti-Flag (clone M2, Sigma), the MAb against HCMV pp28 ...
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bioRxiv - Cell Biology 2024Quote: ... TRPM2-FLAG protein was probed with mouse anti-FLAG®M2 antibodies (F1804, Sigma; primary, 1:5000 in blocking buffer) in conjunction with HRP-conjugated goat anti-mouse IgG (A16078 ...
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bioRxiv - Immunology 2024Quote: ... Plates were washed three times with PBS and incubated 1hr/37°C/5%CO2/rocking with horseradish peroxidase (HRP)-conjugated goat anti-mouse antibody (Sigma), diluted 1:1,500 in blocking buffer ...
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bioRxiv - Immunology 2024Quote: ... membranes were repeatedly washed with TBST and then incubated for 2 h with the anti-Rabbit HRP-conjugated secondary antibody (1:30.000 dilution; Sigma-Aldrich). Immunoreactivity was detected with Clarity Max ECL Substrate (Biorad ...
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bioRxiv - Immunology 2024Quote: Mast cell cultures were stimulated with the following for the indicated time periods: 1 µg/ml IgE-anti DNP antibody (Sigma) and 0.2 µg/ml HSA-DNP (Sigma) ...
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bioRxiv - Cancer Biology 2024Quote: ... was used as the primary antibody and a goat polyclonal anti-rabbit IgG conjugated to horseradish peroxidase (Millipore, 12-348) for the secondary antibody ...
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bioRxiv - Microbiology 2024Quote: ... followed by donkey anti-rabbit secondary antibody conjugated to horseradish peroxidase (“HRP”, Sigma GENA934, 1:5,000 in 5% milk-PBST). Anti-ATS and anti-NapL were detected as previously described (Nacer et al ...
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bioRxiv - Biochemistry 2023Quote: ... FLAG-tagged L protein mutants were detected using horseradish peroxidase-conjugated anti-FLAG M2 antibody (1:9,000) (A8592; Sigma-Aldrich) and bands were visualised by chemiluminescence using Clarity Max Western ECL Substrate (Bio-Rad ...
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bioRxiv - Bioengineering 2023Quote: ... Plates were washed again and anti-FLAG M2 antibody (Sigma-Aldrich, 1:2000 dilution in 5% milk/0.1% PBS-T) was added and incubated for 1hr at room temperature ...
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bioRxiv - Plant Biology 2023Quote: ... 5-day- old light- and dark-grown seedling protein extract was incubated with 1 µl anti- phosphoserine antibody (Sigma, USA) with gentle rotation for 2h at 4°C followed by centrifugation and subjecting the pellet to western blotting as described by Matsushita et al ...
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bioRxiv - Molecular Biology 2023Quote: ... the FLAG-AGO1 proteins were detected with anti-FLAG-HRP antibody (1:4000 [Sigma] in 5 % milk in PBS-T). The membrane was developed as described above with homemade ECL or SuperSignal™ West Femto Maximum Sensitivity Substrate (Thermo Fisher ...
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bioRxiv - Immunology 2022Quote: ... Cells were incubated with Fixable Viability Dye prior staining with fluochrome-conjugated anti-CD4 antibodies and DAPI and acquired on an ImageStreamX appartus from Millipore.
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bioRxiv - Neuroscience 2023Quote: ... the sections were incubated with 0.05M PB containing 0.3% triton X-100 and the primary antibody (rabbit anti-FG, AB153-I, 1:10000, Sigma-Aldrich) at 4°Covernight ...
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bioRxiv - Neuroscience 2023Quote: ... followed by 2 h incubation with HRP-conjugated goat anti-rabbit antibody (1:10,000, Sigma-Aldrich, St. Louis, MO, USA). The signal was visualized by chemiluminescent detection using ECL.
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bioRxiv - Neuroscience 2022Quote: ... membranes were incubated for 1h at room temperature in blocking solution (5% non-fat dried milk and 0.1% Tween 20 in PBS) and then overnight at 4°C with the primary antibody (mouse anti-β-ACTIN, 1:30,000, Sigma; rabbit anti-AKT1 ...
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bioRxiv - Genomics 2022Quote: ... milk powder in TBS-T (Tris-buffered saline-Tween-20) and incubated overnight with primary antibody in 3% BSA/TBS-T (monoclonal mouse anti-FLAG, 1:1,000; Sigma-Aldrich, #F1804-1MG ...
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bioRxiv - Biochemistry 2022Quote: ... Then each membrane was incubated with the corresponding primary antibody (mouse IgG anti-Lewis antigen, Sigma–Aldrich, 1:500 dilution) for 1h at 4°C ...
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bioRxiv - Molecular Biology 2023Quote: ... the elutions containing ORC (typically 2-5) were pooled and incubated with 40 µl of equilibrated Anti-V5-antibody-coupled Agarose Affinity Gel (Sigma) for 30 min at 4°C with rotation ...
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bioRxiv - Molecular Biology 2022Quote: ... and incubated overnight at 4°C with 10μL antibody (anti-SAFB; Bethyl 812-300A; FLAG, Sigma F1804; V5, Sigma V8012) or 10μg of mouse IgG (Invitrogen) ...
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bioRxiv - Molecular Biology 2023Quote: ... BSA for 15 min at room temperature before incubating with primary anti-Flag antibody (diluted with PBS containing 5% BSA at a ratio of 1:300, Sigma) for 1 h at room temperature ...
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bioRxiv - Molecular Biology 2023Quote: ... supplemented with 5% skim milk and incubated for 1 hour with primary antibodies diluted in PBS-Tween (1:1,000 monoclonal anti-Flag M2, F3165, Sigma-Aldrich; 1:1,000 monoclonal anti-GFP antibodies (B-2) ...
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bioRxiv - Microbiology 2023Quote: ... The pre-cleared lysates were then incubated overnight at 4°C with polyclonal rabbit anti-TipR antibodies (1:400 dilution) (Kirkpatrick and Viollier, 2014) or monoclonal rabbit anti-HA antibodies (1:250 dilution) (Clone 114-2C-7, Merck Millipore). ...
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bioRxiv - Neuroscience 2023Quote: ... the sections were blocked with 3% BSA in PBS with 0.3% Triton X-100 for 1h at room temperature and subsequently incubated with primary antibodies (rabbit anti-glutamate, 1:500, Sigma; rabbit anti-SOM ...
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Nucleic acid sensing by STING induces an interferon-like antiviral response in a marine invertebratebioRxiv - Immunology 2022Quote: ... All the samples were analyzed with SDS-PAGE for CBB staining and Western blotting with antibodies of anti-actin (Sigma), anti-LvSTING (Genecreate) ...
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Nucleic acid sensing by STING induces an interferon-like antiviral response in a marine invertebratebioRxiv - Immunology 2022Quote: ... The agarose affinity gels were washed with PBS five times and subjected to SDS-PAGE assay and Western blotting using rabbit anti-GFP antibody (Sigma), rabbit anti-V5 antibody (Merck ...
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bioRxiv - Neuroscience 2023Quote: ... Sections were then incubated for 48–72 h at 4°C with PBS-T containing a combination of the following primary antibodies: rabbit polyclonal anti-CCK (1:1000, Sigma Millipore ...
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bioRxiv - Microbiology 2023Quote: ... protein (1:50 dilution) (BEI Resources, NR-619) and the secondary anti-mouse IgG antibody conjugated with FITC (1:100 dilution, Sigma). The cell nuclei were stained with DAPI ...
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bioRxiv - Molecular Biology 2023Quote: ... We should note that commercially available anti-RPL3L (uL3L) antibodies were also tested (Abcam, ab200646; Biorbyt, orb234958; Sigma-Aldrich, HPA049136) but none of them gave specific bands that were present in WT and absent in Rpl3l-/- samples ...
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bioRxiv - Molecular Biology 2023Quote: ... The expression of the cgs-3xFlag constructs in the resulting Atu strains was confirmed by Western blot using an anti-Flag antibody (Sigma).
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bioRxiv - Neuroscience 2023Quote: ... Retinae were incubated overnight on a rocker at 4 °C with appropriate combinations of the following antibodies: guinea pig anti-RBPMS (1:300, catalog no. ABN1376; Merck Millipore); goat anti-Brn3a (1:100 ...
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bioRxiv - Molecular Biology 2022Quote: ... were washed three times in blocking buffer (0.5% BSA in 1xPBS) and incubated overnight at 4°C with 20μL antibody (anti-FLAG; Sigma F1804-1MG). 30×10^6 of ESCs crosslinked with formaldehyde as described above were resuspended in 500μL RIPA Buffer (50mM Tris-HCl ...
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bioRxiv - Neuroscience 2022Quote: ... membranes were re-probed with the HRP-conjugated primary anti-β-actin antibody for 20 min (Millipore-Sigma, 1:50,000), and the β-actin immunoreactivity was visualized.
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bioRxiv - Plant Biology 2023Quote: ... The sections were blocked with 5% (w/v) bovine serum albumin (BSA) in PBS and incubated then with an anti-HA mouse monoclonal antibody (Sigma) for 2 h at room temperature ...
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bioRxiv - Cancer Biology 2023Quote: ... γ-H2AX foci were detected with mouse monoclonal antibody anti-phospho-Histone H2AX (Ser139) (1:1000 dilution; Cat #05-636, Millipore) and goat anti-mouse IgG secondary antibody ...
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bioRxiv - Cancer Biology 2023Quote: ... Slides were stained with hematoxylin and eosin (H&E) and for Tph1 with an anti-Tph1 primary antibody (Sigma; #T0678). Detection was performed using the polymer-based Envision FLEX+ detection system (Agilent).
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bioRxiv - Genetics 2023Quote: ... the isolated cells were characterized by flow cytometry using the SMC-specific FITC-labeled anti-α-SMA antibody (Sigma, F3777) and an isotype control (mouse IgG2a-FITC ...
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bioRxiv - Microbiology 2023Quote: ... at 1:1000 dilution at room temperature for 1 hour followed by washes x 3 then staining with goat anti-rabbit HRP-conjugated antibody (Catalog #204903, Sigma) at 1:5,000 dilution at room temperature for 1 hour ...
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bioRxiv - Neuroscience 2023Quote: ... Retinae were incubated overnight on a rocker at 4 °C with appropriate combinations of the following antibodies: guinea pig anti-RBPMS (1:300, catalog no. ABN1376; Merck Millipore); goat anti-Brn3a (1:100 ...
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bioRxiv - Neuroscience 2023Quote: ... Then the slides were incubated in the above blocking buffer overnight at room temperature with gentle rocking with the primary antibodies as indicated guinea pig anti-ASIC3 (1:2000, Millipore), rabbit anti-TRPV1 (1:2000 ...
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bioRxiv - Genetics 2023Quote: ... Then cells were resuspended in the BD-FACS staining buffer and labelled through anti-human TRA-1-60 antibody (Millipore) and anti-mouse IgM control ...