Labshake search
Citations for Millipore Sigma :
7301 - 7350 of 10000+ citations for Anti CD152 Antibody since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Molecular Biology 2019Quote: ... the membranes were washed with TBST buffer for three times and subsequently incubated in 1:10,000 dilution of secondary antibody (anti-mouse IgG whole molecule conjugated with alkaline phosphatase) (A3562, Sigma-Aldrich, USA). The membranes were then washed with TBST buffer for three times and detected using AP detection kit (Bio-Rad ...
-
bioRxiv - Bioengineering 2019Quote: ... Tween 20 with 3 % (w/v) skim milk powder and successively incubated with monoclonal mouse anti-T7 RNA polymerase antibodies (Novagen, Merck) and POD labelled goat anti-mouse antibodies (Sigma) ...
-
bioRxiv - Plant Biology 2020Quote: ... Proteins were fractionated on 10% SDS-PAGE gel after boiled in water for 5 min for use in immunoblot analysis with the anti-Flag antibody (Sigma-Aldrich).
-
bioRxiv - Cancer Biology 2019Quote: ... overnight at 4°C and followed by 1-h incubation at room temperature with HRP-conjugated anti-rabbit secondary antibody (1:2000, Sigma Aldrich). Chemiluminescence was detected using ECL Western Blotting substrate (Merck ...
-
bioRxiv - Plant Biology 2019Quote: ... before being rinsed twice for 15 min each in PBS/BSA and incubated for 15 min in a 1/100 dilution (in PBS/BSA) of biotinylated goat anti-rabbit secondary antibody (Sigma Aldrich). Slides were rinsed as before and incubated with a 1/100 dilution (in PBS/BSA ...
-
bioRxiv - Microbiology 2019Quote: ... 3×FLAG- and GST-tagged proteins were detected with a mouse monoclonal HRP-conjugated anti-3×FLAG antibody (M2; Sigma-Aldrich) and a rabbit polyclonal anti-GST antibody (Sigma-Aldrich) ...
-
bioRxiv - Immunology 2019Quote: ... and mouse monoclonal anti-DNA/Histona H1 antibody (1:100; catalog number 05-457/clone AE-4; Merck Millipore, MA, EUA). Alternatively ...
-
bioRxiv - Genomics 2019Quote: ... Expressed TUG1 protein was stained for 1 hour at room temperature using a monoclonal anti-FLAG mouse antibody (1:500, F1804, Sigma Aldrich) and co-stained with organelle markers for mitochondria (1:1000 ...
-
bioRxiv - Cell Biology 2020Quote: ... Detection was carried out by incubating with a 1:5000 dilution of a goat anti-rabbit antibody conjugated to peroxidase (Sigma Aldrich). For tagged proteins detection ...
-
bioRxiv - Cell Biology 2020Quote: ... Detection was carried out by incubating with a 1:4000 dilution of a rabbit anti-rat antibody conjugated to peroxidase (A5795, Sigma-Aldrich). The membranes were then developed with the ECL Plus™ Western blotting detection system (PerkinElmer Life Sciences ...
-
bioRxiv - Bioengineering 2019Quote: ... was incubated in 5% milk powder in TBS-t buffer with anti-tubulin primary antibody (1:500, Sigma #T9026, Buchs, Switzerland)) ...
-
bioRxiv - Pathology 2020Quote: ... and specific proteins were detected by immunostaining with the following antibodies: Cy3-coupled mouse anti-ACTA2 (C6198-100UL, 1:500, Sigma-Aldrich) or biotinylated mouse anti-ACTA2 (MS-113-B0 ...
-
bioRxiv - Developmental Biology 2020Quote: ... terminus or c.terminus anti-SPAG17 antibodies (previously described by Zhang and colleague, Zhang, Jones et al. 2005) and 1:1000 Mouse anti-Tubulin (Sigma #T6199) antibodies) ...
-
bioRxiv - Zoology 2020Quote: ... Each lysate was confirmed to express the appropriate recombinant protein at the expected size using an anti-V5 antibody produced in rabbit (Sigma V8137). Isoform specificity was tested via immunoblotting all cell lysates (empty vector control ...
-
bioRxiv - Pharmacology and Toxicology 2019Quote: ... directed against the N-terminal half of mouse AHR and the polyclonal goat anti-rabbit secondary antibody cross-linked to alkaline phosphatase (Sigma-Aldrich). The blot was developed using the AP Conjugate Substrate Kit (Bio-Rad).
-
bioRxiv - Bioengineering 2020Quote: ... and the membrane was incubated with the secondary antibody (anti-Mouse-HRP IgG, 1:10,000, Sigma-Aldrich, Saint Louis, MO, USA) diluted in TBS-T for 1 hour ...
-
bioRxiv - Molecular Biology 2020Quote: ChIP experiments were performed in AtT-20 cells as previously described (29) using 5 μg of antibody (anti-FLAG-M2, mouse monoclonal, Sigma F3165). ATACseq was performed as previously described (4) ...
-
bioRxiv - Biochemistry 2020Quote: ... N-terminally His-tagged MpPA14 was incubated with the array and binding of the lectin to the polysaccharides was detected by an anti-His tag secondary antibody conjugated to alkaline phosphatase (Sigma-Aldrich). Microarray probing and quantification were performed as previously described(41).
-
bioRxiv - Animal Behavior and Cognition 2020Quote: ... then blocked with 4% NHS/TBST-0.2% and incubated overnight at 4°C in primary antibody solution (1:2500 anti-tyrosine hydroxylase, clone LNC1, Millipore, Billerica, MA) in 2% NHS/TBST-0.2% ...
-
bioRxiv - Biochemistry 2020Quote: ... The supernatant was removed entirely and cells were suspended in 100 μl of the same TBS solution containing mouse monoclonal ANTI-FLAG M1 antibodies (Sigma, F3040) at a 1:500 dilution ...
-
bioRxiv - Molecular Biology 2020Quote: Antibodies and dilutions for IF and Duolink were the following: mouse monoclonal anti-RPAP3 at 1:250 dilution (Sigma-Aldrich, SAB407956); polyclonal rabbit anti-TRBP at 1:100 dilution (Abcam ...
-
bioRxiv - Molecular Biology 2020Quote: ... anti-α-tubulin (T6074, batch number 023M4813. 1/40000), and anti-HA (H6908, batch number 115M4872v. 1/1000) antibodies were purchased from Sigma-Aldrich. Anti-aurora A (30925 ...
-
bioRxiv - Molecular Biology 2021Quote: ... Pre-cleared nuclear lysate was then added to 50 μL beads conjugated with 2-4 μg primary IgG antibody: rabbit anti-APOBEC2 (Sigma, HPA017957) or rabbit IgG isotype control ...
-
bioRxiv - Microbiology 2019Quote: ... Membranes were then washed with TBST buffer (5 x 1 min) and probed with secondary goat anti-rabbit antibodies conjugated with peroxidase (1:1000, Sigma #A6154). Blots were washed as described above and detection was carried out using Amersham ECL Western Blotting Select Detection Reagent (GE Lifescences #RPN2235 ...
-
bioRxiv - Microbiology 2020Quote: ... The secondary solution was prepared with 1% non-fat milk in PBS-T using anti-mouse IgG (whole molecule)–alkaline phosphatase (AP) antibody produced in goat (Sigma-Aldrich) at a dilution of 1:3,000 ...
-
bioRxiv - Bioengineering 2021Quote: ... the cultures were fixed with 4% paraformaldehyde and immunolabeled with anti-neurofilament 200 (NF200) antibodies (1:400, Sigma-Aldrich, catalog # N0142) followed by an Alexa 488 or Alexa 568 conjugated secondary antibody (1:800 ...
-
bioRxiv - Neuroscience 2020Quote: ... followed by incubation at 4°C in a 1:500 dilution of the anti-Calbindin-D-28K antibody (Sigma, CB-955) in blocking solution for 48 hours ...
-
bioRxiv - Pathology 2021Quote: ... The DNP signal associated with RyR was determined using a specific anti-DNP antibody according to the manufacturer’s instructions (Millipore, Billerica, MA). All immunoblots were developed and quantified using an Odyssey system (LI-COR Biosciences ...
-
bioRxiv - Microbiology 2019Quote: ... Detection of FLAG-tagged proteins transferred onto a PVDF membrane was performed with monoclonal anti-FLAG M2-peroxidase (HRP) antibodies (Sigma-Aldrich) and Lumi-Light Western Blotting Substrate Kit (Roche) ...
-
bioRxiv - Neuroscience 2019Quote: ... then labeled with a blend of three FITC-conjugated anti-nucleoprotein monoclonal antibodies (Light Diagnostics Rabies DFA Reagent, EMD Millipore 5100) diluted 1:100 in blocking buffer for 30 minutes ...
-
bioRxiv - Microbiology 2019Quote: For ChIP the following antibodies were used in this study: Rabbit polyclonal anti H3K9/K14-ac (Merck Millipore; Cat#06-599); Rabbit monoclonal anti H3K4-me3 (clone MC315 ...
-
bioRxiv - Immunology 2019Quote: ... Beads were harvested and boiled in 40 μL of Laemmli sample buffer prior to western blot analysis with a polyclonal rabbit anti-PTPN2 antibody (SAB4200249, Sigma-Aldrich).
-
bioRxiv - Microbiology 2019Quote: ... alkaline phosphatase-conjugated (1:10,000) or goat anti-mouse IgG horseradish peroxidase (HRP)-conjugated (1:10,000) secondary antibody (Sigma, St. Louis, MO). Immunoreactive bands were detected by the addition of NBT/BCIP (Sigma ...
-
bioRxiv - Developmental Biology 2019Quote: ... Embryos were then incubated overnight at 4°C in PBST-NGS containing mouse monoclonal anti-α-Tubulin-FITC antibody (Sigma-Aldrich) at a dilution of 1:200 ...
-
bioRxiv - Genetics 2021Quote: ... for 1 hour at 4°C and then incubated overnight on a rotor with 3 μL anti-FLAG antibody (Sigma, F3165). Each reaction was incubated with Protein-G agarose beads (50 μL ...
-
bioRxiv - Genetics 2021Quote: ... was used for staining DNA and mouse anti-a tubulin monoclonal antibody DM1A (1:50) conjugated with FITC (Sigma, St. Louis) was used for staining microtubules ...
-
bioRxiv - Microbiology 2021Quote: ... Expression of both FLAG-tagged AaDNMT2 in mosquito cells was confirmed using qRT-PCR and Western Blots using an anti-FLAG monoclonal antibody (SAB4301135 - Sigma-Aldrich, 1:3000 dilution in 2% Milk in 1X TBS + 1% Tween-20 ...
-
bioRxiv - Physiology 2021Quote: ... Sections were then incubated for 45 minutes at 37°C with the primary antibody at 1:100 for anti-laminin (L9393, Sigma-Aldrich). Three washes were then performed with PBS ...
-
bioRxiv - Physiology 2021Quote: ... Membranes blocked with 5% non-fat milk in PBS-T or Tris-Buffered Saline TBS (TBS-T) (RT, 60min) and immunoblotted using the following primary antibodies (4°C, overnight): rabbit anti-pSmad1/5/8 (Merck Millipore, AB3848), rabbit anti-pSmad2 (Cell Signaling ...
-
bioRxiv - Molecular Biology 2020Quote: ... Unbound protein was washed off in PBST (3x 5 min) and bound protein was detected using HRP-conjugated anti-His antibody (1:2000 in 5% milk PBST; Novagen #71841). After 1 hour incubation at room temperature ...
-
bioRxiv - Molecular Biology 2022Quote: ... Protein samples were resolved using 10% SDS-PAGE and analyzed by Western blotting using anti-c-Myc antibody (Sigma-Aldrich, USA) and anti-PAX9 antibody (Cell signalling technology ...
-
bioRxiv - Developmental Biology 2021Quote: ... and unmodified control (C20) expanded clones were analyzed by Western blot for NCSTN expression with an anti-Nicastrin antibody (N1660, Sigma-Aldrich).
-
bioRxiv - Neuroscience 2021Quote: ... plus 0.1 % Triton X-100 and incubated overnight at 4 °C or for 2 h at room temperature with the following primary antibodies: anti-REST (1:500; 07-579 Millipore, MA), anti-Npas4 (1:500 ...
-
bioRxiv - Neuroscience 2020Quote: ... mouse anti-ubiquitin proteins antibody (clone FK2; Enzo, New York, USA, Cat#BML-PW8810 and Sigma, Gothenburg, Sweden, Cat#04-263), guinea pig polyclonal anti-VGLUT1 (1:5000 ...
-
bioRxiv - Physiology 2021Quote: ... and slides were also counter-stained with Cy3-conjugated anti-mouse alpha smooth muscle Actin SMA-α antibody (A2547 Sigma-Aldrich) at 1/500 ...
-
bioRxiv - Developmental Biology 2020Quote: ... blocked with 3% BSA/TBST for 30 min and incubated with rabbit anti-Sox9 polyclonal antibody (1:500, EMD-Millipore, AB5535), rat anti-endomucin (Emcn ...
-
bioRxiv - Cell Biology 2020Quote: ... pH 7.4) for 1 hour at room temperature and then incubated at 4°C overnight with primary antibodies (anti-multicillin, Sigma Aldrich, HPA058073 1:500 ...
-
bioRxiv - Biochemistry 2021Quote: ... the plates were then washed with PBST and incubated with the detecting antibody: alkaline phosphatase (AP)-conjugated goat anti rabbit for rabbit serum (Sigma-A8025), horseradish peroxidase (HRP)-conjugated anti-M13 antibody (GE healthcare ...
-
bioRxiv - Biochemistry 2021Quote: ... Rabbit anti-SNAP-25 polyclonal antibody raised against a synthetic peptide corresponding to the N-terminus of human SNAP-25 (Sigma S9684), was used as a positive control to detect both cleaved and intact SNAP-25 ...
-
bioRxiv - Neuroscience 2021Quote: ... Slices were transferred to a solution containing anti-Vesicular Glutamate Transporter 2 (VGlut2) polyclonal guinea-pig antibody (AB2251-I Sigma Millipore) in a 1:2000 dilution and kept overnight at 4 °C ...